FvH4_5g23080

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009689
Physical Location & Seq
Forward (+)
14432590 .. 14437139
4550 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g23080.t6

Sequence Viewer

Length: 918 bp
ATGGGGAAGAAAGAAGGAGACTTGTGGGACGATTCGGCTCTCATCAATGCTTTCGACAACGCCATGACTACCTACAAGAAAATGCATAGCAAGAAACCCAGTCAAGGTTCAACCATGGAAGAGACACTTGTGAGCAGTCCTGGAGAACAAGTCATTGCTGCGGCTGACGAAAGCGAGGAGGCGAAAAGAATACAAGGGGATATAGACGACCTGACAAAGGATATGCAGAAGACTACTAAAGAAATGGTAGAGACCAGTAATCTTTCAGATTTACCCGACCCAAATTCACAAGATTATAAGCACATGCAAAACAACTATTCATATTCACAAGGTGGAGAAGATTATACAAAGTTACTCAACGAATATTATGAAATTGAGGAGAAAAGACAGAAGATTCTAGAGCAACTTCATCAATTTGGGGGTGGGAATTACCAGTACTCTGGCGAAGGTTCAGGGTCTGGTGTACAGTGGGGTACTGTTTCTAGTTATGAACAAAATGTAGTCCCTGCTAGCCAACCTTCTAATCCTGCTGTTGTGGGTTCATGTTGCCCGTACATGTGTCCGTGCTTGACGACTTCATGCACTGCATGTCCTGCTTGTTCATCGGATTGGACATGTGCTGGTATAACTTGTACGGAAGGTTGTTTGGCAACAAGTAATGGGAAACCGTTATCTCTTGAAGATGGAAAAATTGTCAAGACAGCAATGGGGACTGCAGAAAGAGCACTATCATCCATGAAAATAAAAGTTACTGGTGATTCTAGTATAACTGAAGAGAAAAAGGAGGAGAAAGATGTAGCGGAAATGGCTGAAACTACTAGCTCAGAGACAGATATCAGTGCGGTACTGAATGCATGGTATTCCGCAGGTTTTTACACTGGCAAGTTTTGCACTGGTCGTATAGAGCCAGGAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMN

Protein Analysis

306

Amino Acids

33.08

Weight (kDa)

4.58

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_G2-BD PF20636 4 - 30 2.1e-13 Survival Motor Neuron, Gemin2-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 857
AciI CCGC 4 cut(s) 161, 800, 842, 864
AcsI RAATTY 1 cut(s) 283
AcuI CTGAAG 1 cut(s) 792
AdeI CACNNNGTG 1 cut(s) 332
AfaI GTAC 6 cut(s) 437, 465, 475, 554, 634, 846
AfiI CCNNNNNNNGG 2 cut(s) 104, 217
AflIII ACRYGT 2 cut(s) 555, 614
AgsI TTSAA 2 cut(s) 111, 680
AjnI CCWGG 2 cut(s) 139, 907
AluBI AGCT 1 cut(s) 822
AluI AGCT 1 cut(s) 822
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 4 cut(s) 12, 116, 245, 821
AlwNI CAGNNNCTG 1 cut(s) 458
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 767
AsuNHI GCTAGC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 145
BccI CCATC 1 cut(s) 677
BciT130I CCWGG 2 cut(s) 141, 909
BcoDI GTCTC 4 cut(s) 12, 116, 245, 821
BfaI CTAG 5 cut(s) 398, 483, 510, 762, 819
BfmI CTRYAG 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 857
BisI GCNGC 2 cut(s) 159, 162
BlsI GCNGC 2 cut(s) 160, 163
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 2 cut(s) 141, 909
BmrFI CCNGG 2 cut(s) 141, 909
BmrI ACTGGG 1 cut(s) 93
BmtI GCTAGC 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 217
Bse1I ACTGG 6 cut(s) 99, 255, 433, 757, 883, 898
Bse3DI GCAATG 2 cut(s) 153, 711
BseBI CCWGG 2 cut(s) 141, 909
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 731
BseLI CCNNNNNNNGG 2 cut(s) 104, 217
BseMI GCAATG 2 cut(s) 153, 711
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 6 cut(s) 99, 255, 433, 757, 883, 898
BseRI GAGGAG 3 cut(s) 191, 392, 800
BseXI GCAGC 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 727
BslFI GGGAC 3 cut(s) 41, 488, 724
BslI CCNNNNNNNGG 2 cut(s) 104, 217
BsmAI GTCTC 4 cut(s) 12, 116, 245, 821
BsmFI GGGAC 3 cut(s) 41, 488, 724
BsmI GAATGC 1 cut(s) 856
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 727
Bsp1407I TGTACA 1 cut(s) 463
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 4 cut(s) 161, 800, 842, 864
BspCNI CTCAG 1 cut(s) 836
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 857
BspOI GCTAGC 1 cut(s) 513
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 153, 711
BsrGI TGTACA 1 cut(s) 463
BsrI ACTGG 6 cut(s) 99, 255, 433, 757, 883, 898
BssECI CCNNGG 1 cut(s) 114
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 141, 909
Bst4CI ACNGT 3 cut(s) 468, 478, 669
Bst6I CTCTTC 2 cut(s) 114, 768
BstAPI GCANNNNNTGC 2 cut(s) 593, 888
BstAUI TGTACA 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 511
BstDEI CTNAG 1 cut(s) 823
BstDSI CCRYGG 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 731
BstMAI GTCTC 4 cut(s) 12, 116, 245, 821
BstMWI GCNNNNNNNGC 4 cut(s) 593, 722, 806, 888
BstNI CCWGG 2 cut(s) 141, 909
BstNSI RCATGY 4 cut(s) 307, 559, 591, 618
BstSCI CCNGG 2 cut(s) 139, 907
BstSFI CTRYAG 1 cut(s) 714
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 440
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 1 cut(s) 731
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 5 cut(s) 473, 582, 844, 876, 891
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 1 cut(s) 511
CaiI CAGNNNCTG 1 cut(s) 458
Csp6I GTAC 6 cut(s) 436, 464, 474, 553, 633, 845
CviJI RGCY 6 cut(s) 38, 164, 513, 809, 822, 907
CviKI_1 RGCY 6 cut(s) 38, 164, 513, 809, 822, 907
CviQI GTAC 6 cut(s) 436, 464, 474, 553, 633, 845
DdeI CTNAG 1 cut(s) 823
DraIII CACNNNGTG 1 cut(s) 332
Eam1104I CTCTTC 2 cut(s) 114, 768
EarI CTCTTC 2 cut(s) 114, 768
Eco130I CCWWGG 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 835
Eco57I CTGAAG 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 139, 907
EcoRV GATATC 1 cut(s) 835
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 2 cut(s) 87, 856
ErhI CCWWGG 1 cut(s) 114
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 3 cut(s) 41, 488, 724
Fnu4HI GCNGC 2 cut(s) 159, 162
FokI GGATG 1 cut(s) 718
Fsp4HI GCNGC 2 cut(s) 159, 162
FspBI CTAG 5 cut(s) 398, 483, 510, 762, 819
GluI GCNGC 2 cut(s) 159, 162
GsuI CTGGAG 1 cut(s) 162
HinfI GANTC 3 cut(s) 32, 394, 758
HphI GGTGA 1 cut(s) 767
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 3 cut(s) 268, 607, 826
Hpy188III TCNNGA 3 cut(s) 398, 677, 697
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 4 cut(s) 8, 440, 528, 632
HpyCH4III ACNGT 3 cut(s) 468, 478, 669
HpyCH4V TGCA 8 cut(s) 85, 226, 307, 582, 587, 716, 854, 891
HpyF10VI GCNNNNNNNGC 4 cut(s) 593, 722, 806, 888
HpyF3I CTNAG 1 cut(s) 823
Lsp1109I GCAGC 1 cut(s) 145
MaeI CTAG 5 cut(s) 398, 483, 510, 762, 819
MaeIII GTNAC 2 cut(s) 351, 748
MboII GAAGA 7 cut(s) 19, 131, 241, 350, 403, 692, 785
MhlI GDGCHC 1 cut(s) 727
MluCI AATT 5 cut(s) 283, 372, 413, 427, 690
MnlI CCTC 4 cut(s) 169, 172, 370, 778
Mph1103I ATGCAT 2 cut(s) 87, 856
MspR9I CCNGG 2 cut(s) 141, 909
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 2 cut(s) 141, 909
MwoI GCNNNNNNNGC 4 cut(s) 593, 722, 806, 888
NcoI CCATGG 1 cut(s) 114
NheI GCTAGC 1 cut(s) 509
NsiI ATGCAT 2 cut(s) 87, 856
NspI RCATGY 4 cut(s) 307, 559, 591, 618
PciI ACATGT 2 cut(s) 555, 614
PctI GAATGC 1 cut(s) 856
PfeI GAWTC 3 cut(s) 32, 394, 758
PfoI TCCNGGA 1 cut(s) 139
PkrI GCNGC 2 cut(s) 160, 163
PscI ACATGT 2 cut(s) 555, 614
PsiI TTATAA 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 139, 907
PspGI CCWGG 2 cut(s) 139, 907
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 458
RsaI GTAC 6 cut(s) 437, 465, 475, 554, 634, 846
RsaNI GTAC 6 cut(s) 436, 464, 474, 553, 633, 845
SatI GCNGC 2 cut(s) 159, 162
ScaI AGTACT 1 cut(s) 437
ScrFI CCNGG 2 cut(s) 141, 909
SduI GDGCHC 1 cut(s) 727
SetI ASST 9 cut(s) 74, 109, 213, 334, 451, 520, 643, 824, 871
SfcI CTRYAG 1 cut(s) 714
Sse9I AATT 5 cut(s) 283, 372, 413, 427, 690
SsiI CCGC 4 cut(s) 161, 800, 842, 864
SspI AATATT 1 cut(s) 365
SspMI CTAG 5 cut(s) 398, 483, 510, 762, 819
StyD4I CCNGG 2 cut(s) 139, 907
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 468, 478, 669
TaqI TCGA 1 cut(s) 54
TasI AATT 5 cut(s) 283, 372, 413, 427, 690
TatI WGTACW 2 cut(s) 435, 463
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 3 cut(s) 32, 394, 758
TscAI CASTG 5 cut(s) 473, 589, 844, 883, 898
TseI GCWGC 1 cut(s) 158
TspDTI ATGAA 8 cut(s) 309, 384, 398, 504, 531, 567, 591, 752
TspGWI ACGGA 2 cut(s) 552, 650
TspRI CASTG 5 cut(s) 473, 589, 844, 883, 898
XagI CCTNNNNNAGG 1 cut(s) 215
XapI RAATTY 1 cut(s) 283
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 4 cut(s) 307, 559, 591, 618
XspI CTAG 5 cut(s) 398, 483, 510, 762, 819
ZrmI AGTACT 1 cut(s) 437
Zsp2I ATGCAT 2 cut(s) 87, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.