RLG00000002968

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009689
Physical Location & Seq
Reverse (-)
42412331 .. 42416706
4376 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002968

Sequence Viewer

Length: 957 bp
ATGGGGAAGAAAGAAGGAGACTTGTGGGACGATTCGGCTCTCGTCAATGCTTTCGACAACGCCATGACTACCTACAAGAAAATGCATAGCAAGAAACCCACTCAAGGTCCAACCCAGGAAGACAAACTTGTGAGCAGTCCTGAAGAACAAGTCATTGTTGAGGCTGGTGAAACCCAGGAGGCTAAACGAATACAAGGTGATGCAGACGACCTGACAAAAGATATGCAGAAGACTACTATAGAAATGGGAGAGACCAGTGATCTCTCAGAAGTTAAAGAAAATCATTGTTTAGATTCATATGTACCCGACTCCTATTCACAAGATGCTCAGGACATGCAAAACAACTATTCGTATACAGAAGGTGGAGAAGATTATACAAAGCTACTCAACCAGTATTACAAGATTGAGGAGAAAAGACAGAAGATTCTAGAGAGACTTCATCAATTTGGTGGTGGGAATTACCAGTATTCTGGCGAAGGTTCAGGTTCTAGTATACAGTGTGGTACTTTTTCTAGTTACGAACAAAATGCAGTCCCTGCAAGCCAACCTTCTAATCCGGCTGTTGTGGGTTCATGTTGCCCGTACATGTGTCCCTGCTTGATGACTTCATGCCCTGCATTTCCTGCTTGTTCATCGGATTGGACATGTGCTGGTACAACTTGTATGGAAGCTTGTTTGACAACAAGTAATGAGAAACCTTTATCTCTTGAAGATGGAAAAATTGTCAAGACAGGAATTGGGACTGCAGAAAGAGCATTATCATCCTTGAAAACAAAAATTACTGGTGATTCTAGTAGAAGTGAAGAGAAAAAGGATGAGAAAGATGAAGCGGAAATGGCTGAAACGACTAGCTCAGAGACAGATATCAGTGTGGTACTGAATGCATGGTATTCCGCAGGTTTCTACACTGGCAAGTATCTTGTGGAGCAATCCATTGCAAAGAAGAGACAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMN

Protein Analysis

319

Amino Acids

34.96

Weight (kDa)

4.64

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_G2-BD PF20636 4 - 30 1.2e-12 Survival Motor Neuron, Gemin2-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 887
AccI GTMKAC 2 cut(s) 353, 493
AciI CCGC 2 cut(s) 830, 894
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 5 cut(s) 303, 505, 584, 655, 876
AfiI CCNNNNNNNGG 1 cut(s) 104
AflIII ACRYGT 2 cut(s) 585, 644
AgsI TTSAA 2 cut(s) 710, 769
AjnI CCWGG 2 cut(s) 114, 174
AluBI AGCT 3 cut(s) 382, 671, 852
AluI AGCT 3 cut(s) 382, 671, 852
Alw26I GTCTC 5 cut(s) 12, 245, 427, 851, 940
AlwNI CAGNNNCTG 1 cut(s) 536
AspS9I GGNCC 1 cut(s) 107
AsuHPI GGTGA 3 cut(s) 179, 209, 797
AvaII GGWCC 1 cut(s) 107
BbsI GAAGAC 2 cut(s) 126, 236
BccI CCATC 1 cut(s) 707
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 2 cut(s) 116, 176
BcoDI GTCTC 5 cut(s) 12, 245, 427, 851, 940
BfaI CTAG 5 cut(s) 428, 489, 513, 792, 849
BfmI CTRYAG 2 cut(s) 237, 744
BfuAI ACCTGC 1 cut(s) 887
Bme1390I CCNGG 2 cut(s) 116, 176
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmrFI CCNGG 2 cut(s) 116, 176
BmsI GCATC 2 cut(s) 190, 313
BpiI GAAGAC 2 cut(s) 126, 236
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 114, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 5 cut(s) 255, 391, 463, 787, 913
Bse3DI GCAATG 1 cut(s) 933
BseBI CCWGG 2 cut(s) 116, 176
BseDI CCNNGG 2 cut(s) 114, 174
BseGI GGATG 2 cut(s) 761, 820
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMI GCAATG 1 cut(s) 933
BseMII CTCAG 3 cut(s) 279, 341, 867
BseNI ACTGG 5 cut(s) 255, 391, 463, 787, 913
BseRI GAGGAG 1 cut(s) 422
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 4 cut(s) 41, 518, 576, 754
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 5 cut(s) 12, 245, 427, 851, 940
BsmFI GGGAC 4 cut(s) 41, 518, 576, 754
BsmI GAATGC 1 cut(s) 886
Bso31I GGTCTC 1 cut(s) 245
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 2 cut(s) 830, 894
BspCNI CTCAG 3 cut(s) 278, 340, 866
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 1 cut(s) 887
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 933
BsrI ACTGG 5 cut(s) 255, 391, 463, 787, 913
BssECI CCNNGG 2 cut(s) 114, 174
BssMI GATC 1 cut(s) 259
BssNAI GTATAC 2 cut(s) 354, 494
Bst1107I GTATAC 2 cut(s) 354, 494
Bst2UI CCWGG 2 cut(s) 116, 176
Bst4CI ACNGT 1 cut(s) 498
Bst6I CTCTTC 2 cut(s) 798, 938
BstAPI GCANNNNNTGC 2 cut(s) 536, 623
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 3 cut(s) 265, 327, 853
BstF5I GGATG 2 cut(s) 761, 820
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 5 cut(s) 12, 245, 427, 851, 940
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 4 cut(s) 536, 623, 752, 836
BstNI CCWGG 2 cut(s) 116, 176
BstNSI RCATGY 3 cut(s) 337, 589, 648
BstSCI CCNGG 2 cut(s) 114, 174
BstSFI CTRYAG 2 cut(s) 237, 744
BstV2I GAAGAC 2 cut(s) 126, 236
BstXI CCANNNNNNTGG 1 cut(s) 470
BstZ17I GTATAC 2 cut(s) 354, 494
BtsCI GGATG 2 cut(s) 761, 820
BtsIMutI CAGTG 4 cut(s) 262, 503, 874, 906
BveI ACCTGC 1 cut(s) 887
Cac8I GCNNGC 1 cut(s) 541
CaiI CAGNNNCTG 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 5 cut(s) 302, 504, 583, 654, 875
CviAII CATG 7 cut(s) 64, 334, 573, 586, 609, 645, 885
CviJI RGCY 9 cut(s) 38, 164, 182, 382, 543, 560, 671, 839, 852
CviKI_1 RGCY 9 cut(s) 38, 164, 182, 382, 543, 560, 671, 839, 852
CviQI GTAC 5 cut(s) 302, 504, 583, 654, 875
DdeI CTNAG 3 cut(s) 265, 327, 853
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
Eam1104I CTCTTC 2 cut(s) 798, 938
EarI CTCTTC 2 cut(s) 798, 938
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 865
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 114, 174
EcoRV GATATC 1 cut(s) 865
EcoT22I ATGCAT 2 cut(s) 87, 886
FaeI CATG 7 cut(s) 67, 337, 576, 589, 612, 648, 888
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 532, 564
FaqI GGGAC 4 cut(s) 41, 518, 576, 754
FatI CATG 7 cut(s) 63, 333, 572, 585, 608, 644, 884
FauNDI CATATG 1 cut(s) 298
FblI GTMKAC 2 cut(s) 353, 493
FokI GGATG 2 cut(s) 748, 827
FspBI CTAG 5 cut(s) 428, 489, 513, 792, 849
HapII CCGG 1 cut(s) 557
Hin1II CATG 7 cut(s) 67, 337, 576, 589, 612, 648, 888
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 5 cut(s) 32, 293, 308, 424, 788
HpaII CCGG 1 cut(s) 557
HphI GGTGA 3 cut(s) 179, 209, 797
Hpy166II GTNNAC 2 cut(s) 354, 494
Hpy188I TCNGA 3 cut(s) 268, 637, 856
Hpy188III TCNNGA 5 cut(s) 140, 329, 428, 707, 727
Hpy8I GTNNAC 2 cut(s) 354, 494
HpyAV CCTTC 4 cut(s) 8, 353, 470, 558
HpyCH4III ACNGT 1 cut(s) 498
HpyF10VI GCNNNNNNNGC 4 cut(s) 536, 623, 752, 836
HpyF3I CTNAG 3 cut(s) 265, 327, 853
Hsp92II CATG 7 cut(s) 67, 337, 576, 589, 612, 648, 888
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 1 cut(s) 925
LweI GCATC 2 cut(s) 190, 313
MaeI CTAG 5 cut(s) 428, 489, 513, 792, 849
MaeIII GTNAC 1 cut(s) 515
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 9 cut(s) 19, 131, 155, 241, 380, 433, 722, 815, 955
MluCI AATT 5 cut(s) 443, 457, 720, 735, 777
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 134
MnlI CCTC 3 cut(s) 154, 172, 400
Mph1103I ATGCAT 2 cut(s) 87, 886
MseI TTAA 2 cut(s) 273, 955
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 2 cut(s) 116, 176
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 116, 176
MwoI GCNNNNNNNGC 4 cut(s) 536, 623, 752, 836
NdeI CATATG 1 cut(s) 298
NdeII GATC 1 cut(s) 259
NlaIII CATG 7 cut(s) 67, 337, 576, 589, 612, 648, 888
NsiI ATGCAT 2 cut(s) 87, 886
NspI RCATGY 3 cut(s) 337, 589, 648
PciI ACATGT 2 cut(s) 585, 644
PctI GAATGC 1 cut(s) 886
PfeI GAWTC 4 cut(s) 32, 293, 424, 788
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PscI ACATGT 2 cut(s) 585, 644
Psp6I CCWGG 2 cut(s) 114, 174
PspGI CCWGG 2 cut(s) 114, 174
PspPI GGNCC 1 cut(s) 107
PstI CTGCAG 1 cut(s) 748
PstNI CAGNNNCTG 1 cut(s) 536
RsaI GTAC 5 cut(s) 303, 505, 584, 655, 876
RsaNI GTAC 5 cut(s) 302, 504, 583, 654, 875
SaqAI TTAA 2 cut(s) 273, 955
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 116, 176
SfaNI GCATC 2 cut(s) 190, 313
SfcI CTRYAG 2 cut(s) 237, 744
SinI GGWCC 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 5 cut(s) 443, 457, 720, 735, 777
SsiI CCGC 2 cut(s) 830, 894
SspMI CTAG 5 cut(s) 428, 489, 513, 792, 849
StyD4I CCNGG 2 cut(s) 114, 174
TaaI ACNGT 1 cut(s) 498
TaqI TCGA 1 cut(s) 54
TasI AATT 5 cut(s) 443, 457, 720, 735, 777
TfiI GAWTC 4 cut(s) 32, 293, 424, 788
Tru1I TTAA 2 cut(s) 273, 955
Tru9I TTAA 2 cut(s) 273, 955
TscAI CASTG 4 cut(s) 262, 503, 874, 913
TspDTI ATGAA 6 cut(s) 285, 428, 561, 597, 621, 840
TspRI CASTG 4 cut(s) 262, 503, 874, 913
VpaK11BI GGWCC 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 3 cut(s) 337, 589, 648
XmiI GTMKAC 2 cut(s) 353, 493
XspI CTAG 5 cut(s) 428, 489, 513, 792, 849
Zsp2I ATGCAT 2 cut(s) 87, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.