Rroxscaffold_3G00247420

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009689
Physical Location & Seq
Reverse (-)
40747264 .. 40753986
6723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247420.1

Sequence Viewer

Length: 1083 bp
ATGGGGAAGAAGGAAGGAGACTTGTGGGACGATTCGGCTCTCATCAATGCTTTCGACAACGCCATGACTACCTACAAGAAAATGCATAGCAAGAAACCCACTCAAGGTCCAACCCAGGAAGACAAACTTGTGAGCAGTCCTGAAGAACAAGTCATTGTTGAGGCTGGTGAAACCCAGGAGGCTAAAAGAGTACAAAGGGATGCAGATGACCTGACAAAAGATATGCAGAAGACTACTATAGAAATGGGAGAGACCAGTAATCTTTCAGAAGTTAAGGAAAATCATTGTTTAGATTCATATGTACCTGACCCCTATTCACAAGATGCTCAGGACAGGCAAAACAACTATTCATATTCAGAAGGTGGAGAAGATTATACAAAGTTACTCGACCAGTATTATGAGATTGAGGAGAAAAGACAGAAGATTCTAGAGAAACTTCATCAATTTGGTGGTGGGAATTACCAGTACTCTGGCGAAGGTTCGGGTTCAGGTTCTGGTGTACAGTGGGGTACTGTTTCTAGTTACGAACAAAATGCAGTCCCTGCTAGCCAACCTTCTAATCCGGCTGTTGTGGGTTCATGTTGCCCGTACATGTGTCCGTGCTTGATGACTTCATGCACTGCATTTCCTGCTTGTCCGTTGGATTCGACATGTGCTGGTATAACTTGTACGGAAGCTTGTTTGGCAACAAGTAATGGCAAACCATTTTCTCTTGAAGATAGAAAAATTGTCAAGACAGCATTTGGGACTGCAGAAAGAGCATTATCATCCATGAAAACAAATATTACTGTTGATTCTAGTATAAGTGAAGAGAAAAAGGATGAGAAAGATGAATCAGAAATGGCTGAAACTACTGGCTCAGAGACAGATATCAGTGTGGTACTGAATGCATGGTATTCCGCAGGTTTCTACACTGGCAAGTATGTATCTTGTGGAGCAATCCATTGCAAAGAAGCGACAAAGTTAAATATTCTCTCGCAACATCAAGCTGCGTGGGACATGTGTCGCAACTTGAACGGGAGACAGGGAAGGAGACACAAGTTTTGGGACAGAGGATTTGATGCCGCCCAAACGGGGCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMN

Protein Analysis

360

Amino Acids

39.67

Weight (kDa)

4.87

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_G2-BD PF20636 4 - 30 2.4e-13 Survival Motor Neuron, Gemin2-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 893
AciI CCGC 2 cut(s) 900, 1065
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 8 cut(s) 192, 303, 467, 501, 511, 590, 670, 882
AfiI CCNNNNNNNGG 2 cut(s) 104, 1074
AflIII ACRYGT 3 cut(s) 591, 650, 999
AgsI TTSAA 2 cut(s) 716, 1015
AjnI CCWGG 2 cut(s) 114, 174
AluBI AGCT 2 cut(s) 677, 989
AluI AGCT 2 cut(s) 677, 989
Alw26I GTCTC 5 cut(s) 12, 245, 857, 1015, 1027
AlwNI CAGNNNCTG 2 cut(s) 494, 542
AoxI GGCC 1 cut(s) 1076
ApeKI GCWGC 1 cut(s) 989
ArsI GACNNNNNNTTYG 4 cut(s) 1026, 1040, 1058, 1072
AspS9I GGNCC 2 cut(s) 107, 1076
AsuHPI GGTGA 1 cut(s) 179
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 1 cut(s) 107
BbsI GAAGAC 2 cut(s) 126, 236
BbvI GCAGC 1 cut(s) 976
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 2 cut(s) 116, 176
BcoDI GTCTC 5 cut(s) 12, 245, 857, 1015, 1027
BfaI CTAG 4 cut(s) 428, 519, 546, 798
BfmI CTRYAG 2 cut(s) 237, 750
BfuAI ACCTGC 1 cut(s) 893
BisI GCNGC 2 cut(s) 990, 1065
BlsI GCNGC 2 cut(s) 991, 1066
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 2 cut(s) 116, 176
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 2 cut(s) 107, 1076
BmiI GGNNCC 1 cut(s) 1077
BmrFI CCNGG 2 cut(s) 116, 176
BmsI GCATC 3 cut(s) 190, 313, 1051
BmtI GCTAGC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 126, 236
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 114, 174
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 1074
Bse1I ACTGG 5 cut(s) 255, 391, 463, 859, 919
Bse3DI GCAATG 1 cut(s) 943
BseBI CCWGG 2 cut(s) 116, 176
BseDI CCNNGG 2 cut(s) 114, 174
BseGI GGATG 3 cut(s) 205, 767, 826
BseLI CCNNNNNNNGG 2 cut(s) 104, 1074
BseMI GCAATG 1 cut(s) 943
BseMII CTCAG 2 cut(s) 341, 873
BseNI ACTGG 5 cut(s) 255, 391, 463, 859, 919
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 1 cut(s) 976
BshFI GGCC 1 cut(s) 1078
BsiSI CCGG 1 cut(s) 563
BslFI GGGAC 5 cut(s) 41, 524, 760, 1010, 1061
BslI CCNNNNNNNGG 2 cut(s) 104, 1074
BsmAI GTCTC 5 cut(s) 12, 245, 857, 1015, 1027
BsmFI GGGAC 5 cut(s) 41, 524, 760, 1010, 1061
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 1 cut(s) 1078
Bso31I GGTCTC 1 cut(s) 245
Bsp1407I TGTACA 1 cut(s) 499
BspACI CCGC 2 cut(s) 900, 1065
BspANI GGCC 1 cut(s) 1078
BspCNI CTCAG 2 cut(s) 340, 872
BspLI GGNNCC 1 cut(s) 1077
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 893
BspOI GCTAGC 1 cut(s) 549
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 943
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 5 cut(s) 255, 391, 463, 859, 919
BssECI CCNNGG 2 cut(s) 114, 174
Bst2UI CCWGG 2 cut(s) 116, 176
Bst4CI ACNGT 3 cut(s) 504, 514, 790
Bst6I CTCTTC 1 cut(s) 804
BstAPI GCANNNNNTGC 2 cut(s) 542, 629
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 547
BstDEI CTNAG 2 cut(s) 327, 859
BstF5I GGATG 3 cut(s) 205, 767, 826
BstMAI GTCTC 5 cut(s) 12, 245, 857, 1015, 1027
BstMWI GCNNNNNNNGC 4 cut(s) 542, 629, 683, 758
BstNI CCWGG 2 cut(s) 116, 176
BstNSI RCATGY 3 cut(s) 595, 654, 1003
BstPAI GACNNNNGTC 1 cut(s) 1002
BstSCI CCNGG 2 cut(s) 114, 174
BstSFI CTRYAG 2 cut(s) 237, 750
BstV1I GCAGC 1 cut(s) 976
BstV2I GAAGAC 2 cut(s) 126, 236
BstXI CCANNNNNNTGG 1 cut(s) 470
BsuRI GGCC 1 cut(s) 1078
BtsCI GGATG 3 cut(s) 205, 767, 826
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 509, 618, 880, 912
BveI ACCTGC 1 cut(s) 893
Cac8I GCNNGC 1 cut(s) 547
CaiI CAGNNNCTG 2 cut(s) 494, 542
Cfr13I GGNCC 2 cut(s) 107, 1076
Csp6I GTAC 8 cut(s) 191, 302, 466, 500, 510, 589, 669, 881
CspCI CAANNNNNGTGG 2 cut(s) 974, 1009
CviAII CATG 8 cut(s) 64, 579, 592, 615, 651, 772, 891, 1000
CviQI GTAC 8 cut(s) 191, 302, 466, 500, 510, 589, 669, 881
DdeI CTNAG 2 cut(s) 327, 859
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 871
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 114, 174
EcoRV GATATC 1 cut(s) 871
EcoT22I ATGCAT 2 cut(s) 87, 892
FaeI CATG 8 cut(s) 67, 582, 595, 618, 654, 775, 894, 1003
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 5 cut(s) 41, 524, 760, 1010, 1061
FatI CATG 8 cut(s) 63, 578, 591, 614, 650, 771, 890, 999
FauNDI CATATG 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 990, 1065
FokI GGATG 3 cut(s) 212, 754, 833
Fsp4HI GCNGC 2 cut(s) 990, 1065
FspBI CTAG 4 cut(s) 428, 519, 546, 798
GluI GCNGC 2 cut(s) 990, 1065
HaeIII GGCC 1 cut(s) 1078
HapII CCGG 1 cut(s) 563
Hin1II CATG 8 cut(s) 67, 582, 595, 618, 654, 775, 894, 1003
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 6 cut(s) 32, 293, 424, 644, 794, 833
HpaII CCGG 1 cut(s) 563
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 4 cut(s) 268, 358, 838, 862
Hpy188III TCNNGA 5 cut(s) 140, 329, 428, 713, 733
Hpy8I GTNNAC 1 cut(s) 500
HpyAV CCTTC 6 cut(s) 4, 8, 353, 470, 564, 1023
HpyCH4III ACNGT 3 cut(s) 504, 514, 790
HpyCH4V TGCA 9 cut(s) 85, 203, 226, 536, 618, 623, 752, 890, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 542, 629, 683, 758
HpyF3I CTNAG 2 cut(s) 327, 859
Hsp92II CATG 8 cut(s) 67, 582, 595, 618, 654, 775, 894, 1003
LmnI GCTCC 1 cut(s) 935
Lsp1109I GCAGC 1 cut(s) 976
LweI GCATC 3 cut(s) 190, 313, 1051
MaeI CTAG 4 cut(s) 428, 519, 546, 798
MaeIII GTNAC 2 cut(s) 381, 521
MboII GAAGA 8 cut(s) 19, 131, 155, 241, 380, 433, 728, 821
MluCI AATT 3 cut(s) 443, 457, 726
MmeI TCCRAC 2 cut(s) 134, 621
MnlI CCTC 4 cut(s) 154, 172, 400, 1046
Mph1103I ATGCAT 2 cut(s) 87, 892
MseI TTAA 2 cut(s) 273, 965
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 2 cut(s) 116, 176
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 2 cut(s) 116, 176
MwoI GCNNNNNNNGC 4 cut(s) 542, 629, 683, 758
NdeI CATATG 1 cut(s) 298
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 8 cut(s) 67, 582, 595, 618, 654, 775, 894, 1003
NlaIV GGNNCC 1 cut(s) 1077
NsiI ATGCAT 2 cut(s) 87, 892
NspI RCATGY 3 cut(s) 595, 654, 1003
PciI ACATGT 3 cut(s) 591, 650, 999
PctI GAATGC 1 cut(s) 892
PfeI GAWTC 6 cut(s) 32, 293, 424, 644, 794, 833
PkrI GCNGC 2 cut(s) 991, 1066
PscI ACATGT 3 cut(s) 591, 650, 999
PshAI GACNNNNGTC 1 cut(s) 1002
Psp6I CCWGG 2 cut(s) 114, 174
PspGI CCWGG 2 cut(s) 114, 174
PspN4I GGNNCC 1 cut(s) 1077
PspPI GGNCC 2 cut(s) 107, 1076
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 494, 542
RsaI GTAC 8 cut(s) 192, 303, 467, 501, 511, 590, 670, 882
RsaNI GTAC 8 cut(s) 191, 302, 466, 500, 510, 589, 669, 881
SaqAI TTAA 2 cut(s) 273, 965
SatI GCNGC 2 cut(s) 990, 1065
Sau96I GGNCC 2 cut(s) 107, 1076
ScaI AGTACT 1 cut(s) 467
ScrFI CCNGG 2 cut(s) 116, 176
SfaNI GCATC 3 cut(s) 190, 313, 1051
SfcI CTRYAG 2 cut(s) 237, 750
SinI GGWCC 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
Sse9I AATT 3 cut(s) 443, 457, 726
SsiI CCGC 2 cut(s) 900, 1065
SspI AATATT 2 cut(s) 784, 970
SspMI CTAG 4 cut(s) 428, 519, 546, 798
StyD4I CCNGG 2 cut(s) 114, 174
TaaI ACNGT 3 cut(s) 504, 514, 790
TaqI TCGA 3 cut(s) 54, 387, 647
TasI AATT 3 cut(s) 443, 457, 726
TatI WGTACW 3 cut(s) 190, 465, 499
TauI GCSGC 1 cut(s) 1067
TfiI GAWTC 6 cut(s) 32, 293, 424, 644, 794, 833
Tru1I TTAA 2 cut(s) 273, 965
Tru9I TTAA 2 cut(s) 273, 965
TscAI CASTG 4 cut(s) 509, 625, 880, 919
TseI GCWGC 1 cut(s) 989
TspDTI ATGAA 7 cut(s) 285, 339, 428, 567, 603, 788, 846
TspGWI ACGGA 3 cut(s) 588, 627, 686
TspRI CASTG 4 cut(s) 509, 625, 880, 919
VpaK11BI GGWCC 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 3 cut(s) 595, 654, 1003
XspI CTAG 4 cut(s) 428, 519, 546, 798
ZrmI AGTACT 1 cut(s) 467
Zsp2I ATGCAT 2 cut(s) 87, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.