Rh7DG268100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009689
Physical Location & Seq
Forward (+)
28634468 .. 28640020
5553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG268100.1

Sequence Viewer

Length: 981 bp
ATGGGGAAGAAAGAAGGAGACTTGTGGGACGATTCGGCTCTCATCAATGCTTTCGACAACGCCATGACTACCTACAAGAAAATGCATAGCAAGAAACCCACTCAAGGTCCAACCCAGGAACACAAACTTGTGAGCAGTCCTGAAGAACAAGTCATTGTTGAGGCTGGTGAAACCCAGGAGGCTAAAAGAATACAAGGGGATGCAGATGACCCAACAAAAGATGTGCAGAAGACTACTATGGAAATGGGAGAGACCAGTAATCTTTCAGAAGCTAAAGAAAATCATTGTTTAGATTCATATGTACCTGACCCCTATTCACAAGATGCTCAGGACATGCAAAACAACTATTCATATTCAGAAGGTGGAGAAGATTATACAAAGTTACTCAACCAGTATTATGAGATTGAGGAGAATAGACAGAAGATTCTAGAGAAACTTCATCAATTTGGTGGTGGGAATTACCAGTATTCTGGCGAAGGTTCAGGTTCAGGTTCTGGTGTACAGTGGGGTACTGTTTCTAGTTACGAACAAAATGCAGTCCCTGCTAGCCAACCTTCTGATCCGGCTGTTGTGGGTTCATGTTGCCCGTACATGTGTCCGTGCTTGATGACTGCATGCACTGCATTTCCTGCTTGTCCGTTGGATTCGACATGTGCTGGTATAACTTGTACGGAAGCTTGTTTTGCAACAAGTAATGGGAAACCATTATCTCTTGAAGATAGAAAAATTGTCAAGACAGCATTTGGGACTGCAGAAAGAGCATTATCATCCATGAAAACAAATATTACTGTTGATTCTAGTAAAAGTGAAGAGAAAGATGAATCAGAAATGGCTGAAACTACTGGCTCAGAGACAGATATCAGTGTGGTACTGAATGCATGGTATTCCGCAGGTTTCTACACTGGCAAGTATGTAATCATTTCCTTACTAAATGATTTCAATCAAATGACTTGTTTCTTCATGCGATTAGCTGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMN

Protein Analysis

326

Amino Acids

35.72

Weight (kDa)

4.52

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_G2-BD PF20636 4 - 30 2.1e-13 Survival Motor Neuron, Gemin2-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 881
AciI CCGC 1 cut(s) 888
AclWI GGATC 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 6 cut(s) 303, 501, 511, 590, 670, 870
AfiI CCNNNNNNNGG 1 cut(s) 104
AflIII ACRYGT 2 cut(s) 591, 650
AgsI TTSAA 2 cut(s) 716, 940
AjnI CCWGG 2 cut(s) 114, 174
AluBI AGCT 3 cut(s) 272, 677, 971
AluI AGCT 3 cut(s) 272, 677, 971
Alw26I GTCTC 3 cut(s) 12, 245, 845
AlwI GGATC 1 cut(s) 554
AlwNI CAGNNNCTG 2 cut(s) 494, 542
AspS9I GGNCC 1 cut(s) 107
AsuHPI GGTGA 1 cut(s) 179
AsuNHI GCTAGC 1 cut(s) 545
AvaII GGWCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 236
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 2 cut(s) 116, 176
BcoDI GTCTC 3 cut(s) 12, 245, 845
BfaI CTAG 4 cut(s) 428, 519, 546, 798
BfmI CTRYAG 1 cut(s) 750
BfuAI ACCTGC 1 cut(s) 881
Bme1390I CCNGG 2 cut(s) 116, 176
Bme18I GGWCC 1 cut(s) 107
BmgT120I GGNCC 1 cut(s) 107
BmrFI CCNGG 2 cut(s) 116, 176
BmsI GCATC 2 cut(s) 190, 313
BmtI GCTAGC 1 cut(s) 549
BpiI GAAGAC 1 cut(s) 236
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 87
BsaBI GATNNNNATC 1 cut(s) 939
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 114, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 5 cut(s) 255, 391, 463, 847, 907
Bse8I GATNNNNATC 1 cut(s) 939
BseBI CCWGG 2 cut(s) 116, 176
BseDI CCNNGG 2 cut(s) 114, 174
BseGI GGATG 2 cut(s) 205, 767
BseJI GATNNNNATC 1 cut(s) 939
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 2 cut(s) 341, 861
BseNI ACTGG 5 cut(s) 255, 391, 463, 847, 907
BseRI GAGGAG 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 245
BsiSI CCGG 1 cut(s) 563
BslFI GGGAC 3 cut(s) 41, 524, 760
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 3 cut(s) 12, 245, 845
BsmFI GGGAC 3 cut(s) 41, 524, 760
BsmI GAATGC 1 cut(s) 880
Bso31I GGTCTC 1 cut(s) 245
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 1 cut(s) 888
BspCNI CTCAG 2 cut(s) 340, 860
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 881
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 5 cut(s) 255, 391, 463, 847, 907
BssECI CCNNGG 2 cut(s) 114, 174
BssMI GATC 1 cut(s) 559
Bst2UI CCWGG 2 cut(s) 116, 176
Bst4CI ACNGT 3 cut(s) 504, 514, 790
Bst6I CTCTTC 1 cut(s) 804
BstAPI GCANNNNNTGC 3 cut(s) 542, 620, 629
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 2 cut(s) 547, 616
BstDEI CTNAG 2 cut(s) 327, 847
BstF5I GGATG 2 cut(s) 205, 767
BstKTI GATC 1 cut(s) 562
BstMAI GTCTC 3 cut(s) 12, 245, 845
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 5 cut(s) 542, 620, 629, 683, 758
BstNI CCWGG 2 cut(s) 116, 176
BstNSI RCATGY 4 cut(s) 337, 595, 618, 654
BstSCI CCNGG 2 cut(s) 114, 174
BstSFI CTRYAG 1 cut(s) 750
BstV2I GAAGAC 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 470
BtsCI GGATG 2 cut(s) 205, 767
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 509, 618, 868, 900
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 2 cut(s) 547, 616
CaiI CAGNNNCTG 2 cut(s) 494, 542
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 6 cut(s) 302, 500, 510, 589, 669, 869
CviAII CATG 9 cut(s) 64, 334, 579, 592, 615, 651, 772, 879, 961
CviQI GTAC 6 cut(s) 302, 500, 510, 589, 669, 869
DdeI CTNAG 2 cut(s) 327, 847
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 859
Eco47I GGWCC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 162
EcoRII CCWGG 2 cut(s) 114, 174
EcoRV GATATC 1 cut(s) 859
EcoT22I ATGCAT 2 cut(s) 87, 880
FaeI CATG 9 cut(s) 67, 337, 582, 595, 618, 654, 775, 882, 964
FaqI GGGAC 3 cut(s) 41, 524, 760
FatI CATG 9 cut(s) 63, 333, 578, 591, 614, 650, 771, 878, 960
FauNDI CATATG 1 cut(s) 298
FokI GGATG 2 cut(s) 212, 754
FspBI CTAG 4 cut(s) 428, 519, 546, 798
HapII CCGG 1 cut(s) 563
Hin1II CATG 9 cut(s) 67, 337, 582, 595, 618, 654, 775, 882, 964
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 6 cut(s) 32, 293, 424, 644, 794, 821
HpaII CCGG 1 cut(s) 563
HphI GGTGA 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 5 cut(s) 268, 358, 559, 826, 850
Hpy188III TCNNGA 5 cut(s) 140, 329, 428, 713, 733
Hpy8I GTNNAC 1 cut(s) 500
HpyAV CCTTC 4 cut(s) 8, 353, 470, 564
HpyCH4III ACNGT 3 cut(s) 504, 514, 790
HpyF10VI GCNNNNNNNGC 5 cut(s) 542, 620, 629, 683, 758
HpyF3I CTNAG 2 cut(s) 327, 847
Hsp92II CATG 9 cut(s) 67, 337, 582, 595, 618, 654, 775, 882, 964
Kzo9I GATC 1 cut(s) 559
LweI GCATC 2 cut(s) 190, 313
MaeI CTAG 4 cut(s) 428, 519, 546, 798
MaeIII GTNAC 2 cut(s) 381, 521
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MboII GAAGA 8 cut(s) 19, 155, 241, 380, 433, 728, 821, 949
MluCI AATT 3 cut(s) 443, 457, 726
MmeI TCCRAC 2 cut(s) 134, 621
MnlI CCTC 3 cut(s) 154, 172, 400
Mph1103I ATGCAT 2 cut(s) 87, 880
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 2 cut(s) 116, 176
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 2 cut(s) 116, 176
MwoI GCNNNNNNNGC 5 cut(s) 542, 620, 629, 683, 758
NdeI CATATG 1 cut(s) 298
NdeII GATC 1 cut(s) 559
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 9 cut(s) 67, 337, 582, 595, 618, 654, 775, 882, 964
NsiI ATGCAT 2 cut(s) 87, 880
NspI RCATGY 4 cut(s) 337, 595, 618, 654
PaeI GCATGC 1 cut(s) 618
PciI ACATGT 2 cut(s) 591, 650
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 6 cut(s) 32, 293, 424, 644, 794, 821
PscI ACATGT 2 cut(s) 591, 650
Psp6I CCWGG 2 cut(s) 114, 174
PspGI CCWGG 2 cut(s) 114, 174
PspPI GGNCC 1 cut(s) 107
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 494, 542
RsaI GTAC 6 cut(s) 303, 501, 511, 590, 670, 870
RsaNI GTAC 6 cut(s) 302, 500, 510, 589, 669, 869
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 1 cut(s) 107
ScrFI CCNGG 2 cut(s) 116, 176
SfaNI GCATC 2 cut(s) 190, 313
SfcI CTRYAG 1 cut(s) 750
SinI GGWCC 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
SphI GCATGC 1 cut(s) 618
Sse9I AATT 3 cut(s) 443, 457, 726
SsiI CCGC 1 cut(s) 888
SspI AATATT 1 cut(s) 784
SspMI CTAG 4 cut(s) 428, 519, 546, 798
StyD4I CCNGG 2 cut(s) 114, 174
TaaI ACNGT 3 cut(s) 504, 514, 790
TaqI TCGA 2 cut(s) 54, 647
TasI AATT 3 cut(s) 443, 457, 726
TatI WGTACW 1 cut(s) 499
TfiI GAWTC 6 cut(s) 32, 293, 424, 644, 794, 821
TscAI CASTG 4 cut(s) 509, 625, 868, 907
TspDTI ATGAA 7 cut(s) 285, 339, 428, 567, 788, 834, 949
TspGWI ACGGA 3 cut(s) 588, 627, 686
TspRI CASTG 4 cut(s) 509, 625, 868, 907
VpaK11BI GGWCC 1 cut(s) 107
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 4 cut(s) 337, 595, 618, 654
XspI CTAG 4 cut(s) 428, 519, 546, 798
Zsp2I ATGCAT 2 cut(s) 87, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.