MD06G1026300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009689
Physical Location & Seq
Reverse (-)
3243598 .. 3248596
4999 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1026300.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGGGAAGCAGGATGGTAACTTGTGGGACGATTCCGCTCTTATCCATGCTTTTGACGATGCCATGTCCAGCTACAAGAAAATTCATAGCAAGAAAACTAATGAGGGTTCAACCAAGGAAGAAAAGGTTGAGATCAGCACAGGAGAACCAGTCATTACAAAGGTTGACGAACATGACGAGGCTAAGAGACAAGGGGATGCAGATGGCCATAGCAGTGATACATTAAAGACTACCGCAGAACTTGGACAGACCACTAATCATTCAGAAGTGAAAGAAAATGATCATGTAGATTTGCATGTACCGGAGCCCTATTTACAAGCTGCACAGGATGCGCAAAATGACTATTCATGTTCACAAGTTACAGAAGATTATAACCAGTTACTCAATCAGTATTATGAGGTTGAGGAGAAAAGACAGAAGCTTCTAGTTCAGCTTCAACAATTCGGTGGTTGGAACTACCAATATTCTGGTGAAGGTTCTGGTTCCGTCATACAAGGCTATACTGATTTTAGCTCTCAGCAGCATGCTCTATATACATGCAATCCTTCTAATCCAGCTGTTGTCTGTTCATGTTGTCCATACCTGTGTCAGTGCTTGATGACTCCATGCACTTCAGTTCCTTCTAGTTTACAGGGGGGATCATGTGCCGGTAAAACTTGTACTGATGCTTGTTTACAAACAAGCTTTGGGAGATCAGTTTCTCTTGAAGATGATAGAATGGTCAAGACAGCAATGGGGACTGCAGAAAGAGCATTATCATCAATGAAAACGAAAATTTCTGGTGATTCTGACATAAGTGAAGATAAAAAGGATGAGAATAGGGAAGGGGAAATGGCTGAAAGTACTAGCTCAGAGACAGATCTTACTGCTGTATTAAATGCATGGTATTCTGCAGGTTTCTATACTGGCAAGTATCTCATGGAGCAATCAGTTGCAAAGAAGCGCCATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SMN

Protein Analysis

318

Amino Acids

34.89

Weight (kDa)

4.79

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMN_G2-BD PF20636 5 - 30 2.8e-12 Survival Motor Neuron, Gemin2-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
Acc16I TGCGCA 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 884
AccBSI CCGCTC 1 cut(s) 38
AciI CCGC 2 cut(s) 36, 234
AclWI GGATC 1 cut(s) 646
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 2 cut(s) 81, 774
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 3 cut(s) 300, 661, 844
AgsI TTSAA 3 cut(s) 111, 437, 707
AluBI AGCT 8 cut(s) 72, 320, 421, 433, 513, 557, 684, 849
AluI AGCT 8 cut(s) 72, 320, 421, 433, 513, 557, 684, 849
Alw26I GTCTC 2 cut(s) 181, 848
AlwI GGATC 1 cut(s) 646
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 2 cut(s) 320, 520
ApoI RAATTY 2 cut(s) 81, 774
AspLEI GCGC 2 cut(s) 334, 945
AsuHPI GGTGA 2 cut(s) 482, 794
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 307, 532
BccI CCATC 2 cut(s) 8, 197
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 181, 848
BfaI CTAG 3 cut(s) 425, 624, 846
BfmI CTRYAG 2 cut(s) 741, 891
BfoI RGCGCY 1 cut(s) 946
BfuAI ACCTGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 859
BisI GCNGC 2 cut(s) 321, 521
BlsI GCNGC 2 cut(s) 322, 522
BmcAI AGTACT 1 cut(s) 844
BmiI GGNNCC 2 cut(s) 306, 484
BmsI GCATC 4 cut(s) 49, 187, 319, 655
BsaJI CCNNGG 2 cut(s) 114, 946
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 3 cut(s) 149, 376, 910
Bse3DI GCAATG 1 cut(s) 738
BseDI CCNNGG 2 cut(s) 114, 946
BseGI GGATG 4 cut(s) 19, 202, 334, 817
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 2 cut(s) 530, 864
BseNI ACTGG 3 cut(s) 149, 376, 910
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 307, 532
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 1 cut(s) 207
BsiSI CCGG 2 cut(s) 302, 648
BslFI GGGAC 2 cut(s) 41, 751
BsmAI GTCTC 2 cut(s) 181, 848
BsmFI GGGAC 2 cut(s) 41, 751
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 5 cut(s) 132, 280, 638, 692, 859
Bsp19I CCATGG 1 cut(s) 946
BspACI CCGC 2 cut(s) 36, 234
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 529, 863
BspLI GGNNCC 2 cut(s) 306, 484
BspMAI CTGCAG 2 cut(s) 745, 895
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 1 cut(s) 646
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 1 cut(s) 738
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 3 cut(s) 149, 376, 910
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 2 cut(s) 114, 946
BssMI GATC 5 cut(s) 132, 280, 638, 692, 859
BssT1I CCWWGG 2 cut(s) 114, 946
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 3 cut(s) 183, 516, 850
BstDSI CCRYGG 1 cut(s) 946
BstF5I GGATG 4 cut(s) 19, 202, 334, 817
BstH2I RGCGCY 1 cut(s) 946
BstHHI GCGC 2 cut(s) 334, 945
BstKTI GATC 5 cut(s) 135, 283, 641, 695, 862
BstMAI GTCTC 2 cut(s) 181, 848
BstMBI GATC 5 cut(s) 132, 280, 638, 692, 859
BstMWI GCNNNNNNNGC 2 cut(s) 329, 749
BstNSI RCATGY 3 cut(s) 299, 527, 540
BstSFI CTRYAG 2 cut(s) 741, 891
BstV1I GCAGC 2 cut(s) 307, 532
BstX2I RGATCY 1 cut(s) 859
BstXI CCANNNNNNTGG 1 cut(s) 467
BstYI RGATCY 1 cut(s) 859
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 946
BtsCI GGATG 4 cut(s) 19, 202, 334, 817
BtsI GCAGTG 1 cut(s) 220
BtsIMutI CAGTG 2 cut(s) 220, 596
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 1 cut(s) 525
CfoI GCGC 2 cut(s) 334, 945
Cfr10I RCCGGY 1 cut(s) 647
Csp6I GTAC 3 cut(s) 299, 660, 843
CviQI GTAC 3 cut(s) 299, 660, 843
DdeI CTNAG 3 cut(s) 183, 516, 850
DpnI GATC 5 cut(s) 134, 282, 640, 694, 861
DpnII GATC 5 cut(s) 132, 280, 638, 692, 859
EaeI YGGCCR 1 cut(s) 205
Eco130I CCWWGG 2 cut(s) 114, 946
Eco24I GRGCYC 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 597
EcoT14I CCWWGG 2 cut(s) 114, 946
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 2 cut(s) 114, 946
FaqI GGGAC 2 cut(s) 41, 751
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 2 cut(s) 321, 521
FokI GGATG 4 cut(s) 26, 209, 341, 824
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 321, 521
FspBI CTAG 3 cut(s) 425, 624, 846
FspI TGCGCA 1 cut(s) 333
GlaI GCGC 2 cut(s) 333, 944
GluI GCNGC 2 cut(s) 321, 521
HaeII RGCGCY 1 cut(s) 946
HaeIII GGCC 1 cut(s) 207
HapII CCGG 2 cut(s) 302, 648
HhaI GCGC 2 cut(s) 334, 945
Hin6I GCGC 2 cut(s) 332, 943
HinP1I GCGC 2 cut(s) 332, 943
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 2 cut(s) 419, 682
HinfI GANTC 3 cut(s) 32, 601, 785
HpaII CCGG 2 cut(s) 302, 648
HphI GGTGA 2 cut(s) 482, 794
Hpy166II GTNNAC 4 cut(s) 166, 353, 629, 674
Hpy188I TCNGA 3 cut(s) 265, 790, 853
Hpy188III TCNNGA 2 cut(s) 704, 724
Hpy8I GTNNAC 4 cut(s) 166, 353, 629, 674
HpyAV CCTTC 4 cut(s) 467, 555, 630, 818
HpyCH4V TGCA 9 cut(s) 200, 295, 323, 540, 609, 743, 881, 893, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 749
HpyF3I CTNAG 3 cut(s) 183, 516, 850
HspAI GCGC 2 cut(s) 332, 943
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 5 cut(s) 132, 280, 638, 692, 859
LmnI GCTCC 2 cut(s) 304, 922
Lsp1109I GCAGC 2 cut(s) 307, 532
LweI GCATC 4 cut(s) 49, 187, 319, 655
MaeI CTAG 3 cut(s) 425, 624, 846
MaeIII GTNAC 3 cut(s) 17, 358, 378
MalI GATC 5 cut(s) 134, 282, 640, 694, 861
MbiI CCGCTC 1 cut(s) 38
MboI GATC 5 cut(s) 132, 280, 638, 692, 859
MboII GAAGA 4 cut(s) 131, 377, 719, 812
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 3 cut(s) 81, 440, 774
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 4 cut(s) 97, 172, 391, 397
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 883
MscI TGGCCA 1 cut(s) 207
MseI TTAA 3 cut(s) 224, 875, 952
MslI CAYNNNNRTG 2 cut(s) 213, 583
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 557
MspI CCGG 2 cut(s) 302, 648
MwoI GCNNNNNNNGC 2 cut(s) 329, 749
NcoI CCATGG 1 cut(s) 946
NdeII GATC 5 cut(s) 132, 280, 638, 692, 859
NlaIV GGNNCC 2 cut(s) 306, 484
NsbI TGCGCA 1 cut(s) 333
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 3 cut(s) 299, 527, 540
PaeI GCATGC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 2 cut(s) 32, 785
PkrI GCNGC 2 cut(s) 322, 522
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PsiI TTATAA 1 cut(s) 372
PspN4I GGNNCC 2 cut(s) 306, 484
PstI CTGCAG 2 cut(s) 745, 895
PsuI RGATCY 1 cut(s) 859
PvuII CAGCTG 1 cut(s) 557
RsaI GTAC 3 cut(s) 300, 661, 844
RsaNI GTAC 3 cut(s) 299, 660, 843
RseI CAYNNNNRTG 2 cut(s) 213, 583
SaqAI TTAA 3 cut(s) 224, 875, 952
SatI GCNGC 2 cut(s) 321, 521
Sau3AI GATC 5 cut(s) 132, 280, 638, 692, 859
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 1 cut(s) 595
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 4 cut(s) 49, 187, 319, 655
SfcI CTRYAG 2 cut(s) 741, 891
SmiMI CAYNNNNRTG 2 cut(s) 213, 583
SphI GCATGC 1 cut(s) 527
Sse9I AATT 3 cut(s) 81, 440, 774
SsiI CCGC 2 cut(s) 36, 234
SspI AATATT 1 cut(s) 464
SspMI CTAG 3 cut(s) 425, 624, 846
StyI CCWWGG 2 cut(s) 114, 946
TasI AATT 3 cut(s) 81, 440, 774
TatI WGTACW 2 cut(s) 659, 842
TfiI GAWTC 2 cut(s) 32, 785
Tru1I TTAA 3 cut(s) 224, 875, 952
Tru9I TTAA 3 cut(s) 224, 875, 952
TscAI CASTG 2 cut(s) 220, 596
TseI GCWGC 2 cut(s) 320, 520
TspDTI ATGAA 4 cut(s) 74, 336, 558, 779
TspGWI ACGGA 1 cut(s) 475
TspRI CASTG 2 cut(s) 220, 596
XapI RAATTY 2 cut(s) 81, 774
XceI RCATGY 3 cut(s) 299, 527, 540
XspI CTAG 3 cut(s) 425, 624, 846
ZrmI AGTACT 1 cut(s) 844
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.