FvH4_5g25860

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010739
Physical Location & Seq
Forward (+)
17346115 .. 17350543
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25860.t1

Sequence Viewer

Length: 1539 bp
ATGGCTGGCGGTGGTGGTGGCCCTGACAGGGAGCTCGATCAGACTCCGACGTGGGCTGTCGCCGGTGTCTGTTTCACTATCATTGTTATTTCTATAGCTTTAGAGAAGATTATTCACCTGGTCGGACATTGGTTTCAACGTAGAAAAAAAGCTGGTTTGCTTGAAGCTCTTGAGATAGTTAAAGCGGAGCTTATGGTTTTGGGATTCATTTCTTTGATACTAACATTTGCACAACAGCACATCGCTCAGTTGTGCATTCCTGTAAGCTATGCCAATACGATGTTGCCTTGTCCTTACCATAAGTCTGAGGATCACAGTGGTGGTGGTGGTAAAGGTGGTGGAGGAGGAGGAGGTCATCGCAGGCTCTTATGGTATGAGCAGGAGCGCAGAATTTTAGCTGCTGGTAGCATGGGCGCAGGCTGTTCGAAGGGAAAAGTGCCGCTTATCTCTATAAATGGATTACACCAGTTGCACATCTTCATCTTCTTCTTAGCAGTATTTCACGTTGTATATGGTGCCATAACCATGACACTGGGAAGATTAAAGATTCGTGAGTGGAAGAAGTGGGAACGAGAAGCTGTAAATGATCAGGACTTCAATGATCCTACGAAATTTAGGCTTACTCACGAGACATCATTTGTGAGAGACCACACAAAATCTTGGACTAAAACAAAATTCACCTTCTACTTTGTAAGCTTTTTTCGACAATTCTTTAGGTCTGTTCGTAGGGCTGATTTCTTGACCATGCGACACGGATTCGTCTCTGTTCATTTAGCACCCGGAAGCAAGTTTGATTTTCAAAAATATATCAAAAGATCGTTAGAAGATGACTTTAAGGTGGTTGTGGGAATCAGTCCACTGTTATGGACTACGATGACGCTATTTTTGCTTCTCAATGTTCATGGATGGGAGGCTATGTTTGTCTTGTCCATGCTTCCTCTAGTTATAATCTTAGCTGTTGGAACAAAGCTGCAAGGAATTATAACTCAAATGGCAATTGAAATCCAAGAAAGACATGCTGTGGTGCAAGGGATACCTCTGGTGCAAGTCACTGATAAGCATTTTTGGTTCAGCTGGCCGGAGCTGGTGCTTTATTTGATCCATTTTGTCCTGTTCCAGAATGCCTTTGAGATAACATATTTCTTTTGGATATGGTACGAGTTTGGGGTGAAATCTTGTTTTCATGAAAACTTCACTCTTACGATTATTAGAGTTGCTCTTGGGGTCCTCGCCCAGATTTTGTGTAGCTACATCACACTTCCGCTATATGCATTGGTTACACAGATGGGTTCAACAATGAAGAAGTCGATCTTCGATGAACAAACTAACAAGGCCTTGAAGAAATGGCAAAAGAATGCTCAAAAGAAGAATAAGAATGAGACTGGCTCACACAATATAACAACTAAACCTGTGCTGGGTGGGAATTCAACCAATGGACAAACTGACATGGAGAAAGATGGAGCAGATCCACATGAAATGGAAAACCCTTTTGCCAGTGTCAATGTTGAAGGCAATAAAGACCTCCTAACAGGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

513

Amino Acids

58.0

Weight (kDa)

9.21

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 468 2.1e-209 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010739)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42560 AT1G42560 AT2G33670
fragaria_vesca FvH4_5g25860 FvH4_5g25860
malus_domestica MD06G1005800.v1.1 MD10G1233000.v1.1 MD11G1034900.v1.1 MD17G1274600.v1.1
prunus_persica Prupe.5G007100_v2.0.a1
pyrus_communis pycom04g09600 pycom05g07420
rosa_chinensis RchiOBHm_Chr7g0216341
rosa_laevigata RLG00000002580
rosa_roxburghii Rroxscaffold_3G00243310
rosa_rugosa Rorug07G0160000
rosa_samantha Rh7AG297500 Rh7CG317700 Rh7DG299900
rosa_wichuraiana Rw7G025310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 947, 983
AccB1I GGYRCC 1 cut(s) 515
AciI CCGC 4 cut(s) 9, 185, 440, 1262
AclWI GGATC 4 cut(s) 318, 596, 1093, 1460
AcoI YGGCCR 1 cut(s) 1076
AcsI RAATTY 4 cut(s) 390, 611, 674, 1423
AfaI GTAC 1 cut(s) 1157
AfiI CCNNNNNNNGG 2 cut(s) 28, 1415
AgsI TTSAA 9 cut(s) 137, 164, 598, 800, 1001, 1293, 1339, 1428, 1508
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 117
AleI CACNNNNGTG 1 cut(s) 318
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 4 cut(s) 623, 639, 766, 1373
AlwI GGATC 4 cut(s) 318, 596, 1093, 1460
AoxI GGCC 3 cut(s) 19, 1076, 1332
ApeKI GCWGC 2 cut(s) 398, 970
ApoI RAATTY 4 cut(s) 390, 611, 674, 1423
ArsI GACNNNNNNTTYG 2 cut(s) 868, 900
AspLEI GCGC 2 cut(s) 387, 416
AspS9I GGNCC 2 cut(s) 20, 1225
AsuC2I CCSGG 1 cut(s) 780
AsuHPI GGTGA 3 cut(s) 107, 670, 1180
AsuII TTCGAA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 1225
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 36
BauI CACGAG 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 385, 957
BccI CCATC 3 cut(s) 900, 1279, 1451
BcgI CGANNNNNNTGC 2 cut(s) 405, 439
BciT130I CCWGG 1 cut(s) 119
BciVI GTATCC 1 cut(s) 1026
BclI TGATCA 1 cut(s) 586
BcnI CCSGG 1 cut(s) 780
BcoDI GTCTC 4 cut(s) 623, 639, 766, 1373
BfaI CTAG 1 cut(s) 941
BfmI CTRYAG 1 cut(s) 93
BfuI GTATCC 1 cut(s) 1026
BisI GCNGC 3 cut(s) 399, 440, 971
BlsI GCNGC 3 cut(s) 400, 441, 972
Bme1390I CCNGG 2 cut(s) 119, 780
Bme18I GGWCC 1 cut(s) 1225
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 20, 1225
BmiI GGNNCC 2 cut(s) 517, 1226
BmrFI CCNGG 2 cut(s) 119, 780
BmrI ACTGGG 1 cut(s) 542
BmuI ACTGGG 1 cut(s) 542
BplI GAGNNNNNCTC 2 cut(s) 1370, 1402
Bpu14I TTCGAA 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 780
BsaI GGTCTC 1 cut(s) 639
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 1415
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 4 cut(s) 466, 537, 1387, 1494
BseBI CCWGG 1 cut(s) 119
BseGI GGATG 1 cut(s) 911
BseLI CCNNNNNNNGG 2 cut(s) 28, 1415
BseMII CTCAG 2 cut(s) 260, 297
BseNI ACTGG 4 cut(s) 466, 537, 1387, 1494
BseRI GAGGAG 3 cut(s) 357, 360, 363
BseXI GCAGC 2 cut(s) 385, 957
BseYI CCCAGC 1 cut(s) 1414
BshFI GGCC 3 cut(s) 21, 1078, 1334
BshNI GGYRCC 1 cut(s) 515
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 3 cut(s) 63, 780, 1079
BslI CCNNNNNNNGG 2 cut(s) 28, 1415
BsmAI GTCTC 4 cut(s) 623, 639, 766, 1373
BsmBI CGTCTC 1 cut(s) 766
BsmI GAATGC 3 cut(s) 255, 1126, 1360
BsnI GGCC 3 cut(s) 21, 1078, 1334
Bso31I GGTCTC 1 cut(s) 639
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 36
Bsp143I GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
BspACI CCGC 4 cut(s) 9, 185, 440, 1262
BspANI GGCC 3 cut(s) 21, 1078, 1334
BspCNI CTCAG 2 cut(s) 259, 298
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 2 cut(s) 517, 1226
BspPI GGATC 4 cut(s) 318, 596, 1093, 1460
BspT104I TTCGAA 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 639
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 4 cut(s) 466, 537, 1387, 1494
BssAI RCCGGY 1 cut(s) 62
BssMI GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
BssSI CACGAG 1 cut(s) 626
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 317, 861, 1536
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 4 cut(s) 7, 362, 418, 1076
BstDEI CTNAG 4 cut(s) 246, 306, 490, 952
BstF5I GGATG 1 cut(s) 911
BstHHI GCGC 2 cut(s) 387, 416
BstKTI GATC 8 cut(s) 40, 313, 589, 604, 818, 1101, 1311, 1468
BstMAI GTCTC 4 cut(s) 623, 639, 766, 1373
BstMBI GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
BstMWI GCNNNNNNNGC 1 cut(s) 886
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 1 cut(s) 1019
BstSCI CCNGG 2 cut(s) 117, 778
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 385, 957
BstX2I RGATCY 1 cut(s) 1465
BstXI CCANNNNNNTGG 2 cut(s) 532, 864
BstYI RGATCY 1 cut(s) 1465
BsuI GTATCC 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 21, 1078, 1334
BtgZI GCGATG 2 cut(s) 226, 341
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 911
BtsIMutI CAGTG 5 cut(s) 322, 530, 857, 1050, 1501
Cac8I GCNNGC 4 cut(s) 7, 362, 418, 1076
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 2 cut(s) 387, 416
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 20, 1225
CseI GACGC 1 cut(s) 886
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 1 cut(s) 1156
CviAII CATG 9 cut(s) 409, 526, 745, 902, 931, 1016, 1184, 1447, 1472
CviQI GTAC 1 cut(s) 1156
DdeI CTNAG 4 cut(s) 246, 306, 490, 952
DpnI GATC 8 cut(s) 39, 312, 588, 603, 817, 1100, 1310, 1467
DpnII GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
EaeI YGGCCR 1 cut(s) 1076
Ecl136II GAGCTC 1 cut(s) 34
Eco147I AGGCCT 1 cut(s) 1334
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 639
Eco47I GGWCC 1 cut(s) 1225
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 1225
EcoRI GAATTC 1 cut(s) 1423
EcoRII CCWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 1273
EcoT38I GRGCYC 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 766
FaeI CATG 9 cut(s) 412, 529, 748, 905, 934, 1019, 1187, 1450, 1475
FalI AAGNNNNNCTT 6 cut(s) 174, 206, 426, 458, 1295, 1327
FatI CATG 9 cut(s) 408, 525, 744, 901, 930, 1015, 1183, 1446, 1471
FbaI TGATCA 1 cut(s) 586
Fnu4HI GCNGC 3 cut(s) 399, 440, 971
FokI GGATG 1 cut(s) 918
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 3 cut(s) 399, 440, 971
FspBI CTAG 1 cut(s) 941
GlaI GCGC 2 cut(s) 386, 415
GluI GCNGC 3 cut(s) 399, 440, 971
GsaI CCCAGC 1 cut(s) 1418
HaeIII GGCC 3 cut(s) 21, 1078, 1334
HapII CCGG 3 cut(s) 63, 780, 1079
HgaI GACGC 1 cut(s) 886
HhaI GCGC 2 cut(s) 387, 416
Hin1II CATG 9 cut(s) 412, 529, 748, 905, 934, 1019, 1187, 1450, 1475
Hin6I GCGC 2 cut(s) 385, 414
HinP1I GCGC 2 cut(s) 385, 414
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 5 cut(s) 43, 204, 547, 756, 849
HpaII CCGG 3 cut(s) 63, 780, 1079
HphI GGTGA 3 cut(s) 107, 670, 1180
Hpy166II GTNNAC 1 cut(s) 857
Hpy188I TCNGA 4 cut(s) 42, 48, 125, 307
Hpy188III TCNNGA 7 cut(s) 170, 551, 590, 626, 739, 1117, 1184
Hpy8I GTNNAC 1 cut(s) 857
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 3 cut(s) 421, 691, 1502
HpyCH4III ACNGT 3 cut(s) 317, 861, 1536
HpyCH4IV ACGT 3 cut(s) 50, 139, 504
HpyCH4V TGCA 7 cut(s) 230, 255, 472, 973, 1027, 1045, 1271
HpyF10VI GCNNNNNNNGC 1 cut(s) 886
HpyF3I CTNAG 4 cut(s) 246, 306, 490, 952
HpySE526I ACGT 3 cut(s) 50, 139, 504
Hsp92II CATG 9 cut(s) 412, 529, 748, 905, 934, 1019, 1187, 1450, 1475
HspAI GCGC 2 cut(s) 385, 414
Ksp22I TGATCA 1 cut(s) 586
Kzo9I GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
LmnI GCTCC 5 cut(s) 31, 187, 382, 1081, 1460
Lsp1109I GCAGC 2 cut(s) 385, 957
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 1 cut(s) 941
MaeII ACGT 3 cut(s) 50, 139, 504
MaeIII GTNAC 2 cut(s) 1048, 1276
MalI GATC 8 cut(s) 39, 312, 588, 603, 817, 1100, 1310, 1467
MboI GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 1 cut(s) 1465
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 7 cut(s) 390, 611, 674, 707, 978, 996, 1423
MlyI GAGTC 1 cut(s) 37
MmeI TCCRAC 3 cut(s) 71, 103, 940
Mph1103I ATGCAT 1 cut(s) 1273
MseI TTAA 3 cut(s) 180, 542, 834
MslI CAYNNNNRTG 3 cut(s) 318, 524, 862
MspA1I CMGCKG 1 cut(s) 1074
MspI CCGG 3 cut(s) 63, 780, 1079
MspR9I CCNGG 2 cut(s) 119, 780
MunI CAATTG 1 cut(s) 996
Mva1269I GAATGC 3 cut(s) 255, 1126, 1360
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 886
NciI CCSGG 1 cut(s) 780
NdeII GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
NlaIII CATG 9 cut(s) 412, 529, 748, 905, 934, 1019, 1187, 1450, 1475
NlaIV GGNNCC 2 cut(s) 517, 1226
NmuCI GTSAC 1 cut(s) 1048
NsiI ATGCAT 1 cut(s) 1273
NspI RCATGY 1 cut(s) 1019
NspV TTCGAA 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 318
PagI TCATGA 1 cut(s) 1183
PceI AGGCCT 1 cut(s) 1334
PctI GAATGC 3 cut(s) 255, 1126, 1360
PfeI GAWTC 4 cut(s) 204, 547, 756, 849
PkrI GCNGC 3 cut(s) 400, 441, 972
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 1225
PsiI TTATAA 2 cut(s) 947, 983
Psp124BI GAGCTC 1 cut(s) 36
Psp5II RGGWCCY 1 cut(s) 1225
Psp6I CCWGG 1 cut(s) 117
PspFI CCCAGC 1 cut(s) 1414
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 517, 1226
PspPI GGNCC 2 cut(s) 20, 1225
PspPPI RGGWCCY 1 cut(s) 1225
PsuI RGATCY 1 cut(s) 1465
PvuII CAGCTG 1 cut(s) 1074
RsaI GTAC 1 cut(s) 1157
RsaNI GTAC 1 cut(s) 1156
RseI CAYNNNNRTG 3 cut(s) 318, 524, 862
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 180, 542, 834
SatI GCNGC 3 cut(s) 399, 440, 971
Sau3AI GATC 8 cut(s) 37, 310, 586, 601, 815, 1098, 1308, 1465
Sau96I GGNCC 2 cut(s) 20, 1225
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 2 cut(s) 119, 780
SduI GDGCHC 1 cut(s) 36
SexAI ACCWGGT 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 425
SgrAI CRCCGGYG 1 cut(s) 62
SinI GGWCC 1 cut(s) 1225
SmiMI CAYNNNNRTG 3 cut(s) 318, 524, 862
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 7 cut(s) 390, 611, 674, 707, 978, 996, 1423
SseBI AGGCCT 1 cut(s) 1334
SsiI CCGC 4 cut(s) 9, 185, 440, 1262
SspMI CTAG 1 cut(s) 941
SstI GAGCTC 1 cut(s) 36
StuI AGGCCT 1 cut(s) 1334
StyD4I CCNGG 2 cut(s) 117, 778
TaaI ACNGT 3 cut(s) 317, 861, 1536
TaiI ACGT 3 cut(s) 53, 142, 507
TaqI TCGA 5 cut(s) 36, 425, 703, 1307, 1314
TasI AATT 7 cut(s) 390, 611, 674, 707, 978, 996, 1423
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 4 cut(s) 204, 547, 756, 849
Tru1I TTAA 3 cut(s) 180, 542, 834
Tru9I TTAA 3 cut(s) 180, 542, 834
TscAI CASTG 5 cut(s) 322, 537, 864, 1057, 1501
TseFI GTSAC 1 cut(s) 1048
TseI GCWGC 2 cut(s) 398, 970
Tsp45I GTSAC 1 cut(s) 1048
TspDTI ATGAA 9 cut(s) 196, 469, 758, 890, 1172, 1200, 1313, 1332, 1488
TspGWI ACGGA 1 cut(s) 768
TspRI CASTG 5 cut(s) 322, 537, 864, 1057, 1501
VpaK11BI GGWCC 1 cut(s) 1225
XapI RAATTY 4 cut(s) 390, 611, 674, 1423
XceI RCATGY 1 cut(s) 1019
XspI CTAG 1 cut(s) 941
Zsp2I ATGCAT 1 cut(s) 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.