MD06G1005800.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010739
Physical Location & Seq
Reverse (-)
610672 .. 616230
5559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1005800.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGGCGGGTGGAGGAGGGGGTAATGCCGGCAGAGAGCTCGATCAGACGCCAACATGGGCTCTGGCCGCCGTCTGCTTCATTATCATTATTATTTCTATTGTTTTGGAAAAGGTTCTTCACATGGTTGGAGAGTTTTTTCATCATAAAAAGAAGGTGGGTTTGCTTGAAGCTCTTGAGAAAGTGAAAGGGGAGCTTATGGTTTTGGGATTTATATCTCTGTTATTGACATTTGGGCAAAAGTATATATCTCAAGTGTGTATTCCCATAGAGGCTGCAGACACAATGTTGCCTTGCCCGTACAGAGACCCTGACGAAGAAGCAGGTGGTGGTGGGGGTGGGGGTGACCATCGTCGTAGGCTTTTATGGTATGAACGCAGATATTTAGCTGGTGGTAGCGCAGGTCCTGGCTGCAAAGCGGGAAAAGTGCCATTGATATCTTTAAATGGATTACACCAACTGCACATCTTCATATTCTTTTTGGCAATATTTCACGTTGTTTACGGTGCGTTAACAATGACCCTTGGAAGATTAAAGATTCGTGCTTGGAAGGTGTGGGAACGAGAGATGGAAAATGATAATGAATTCAATGATCCTACAAAATTCAGGCTCACTCACGAGACATCGTTTGTGAGAGACCACACCAAGTGTTGGACTAAAACACCATTCACCTTCTATCTTGTAAGCTTTTTCAGACAATTCTTTAGGTCTGTTCGTAGGGCTGACTACTTGACCATGCGACATGGGTTCATCGCTGTTCATTTAGCACCTGGAAGTAAGTTTGACTTTCAAAAATATATCAAAAGGTCATTAGAAGATGACTTTAAGGTGGTTGTGGGAATCACTCCACTCTTATGGACTACAATGACTCTCTACTTGCTTCTCAATGTTCATGGATGGGAAGCTATGTTTGTGCTATCCATACTTCCTCTAATTATAATCTTAGCTGTTGGAACAAAGCTGCAAGCAATTATAACACAAATGGCACTTGAAATCCAAGAAAGGCATGCTGTAGTCCAAGGAATACCTCTGGTGCAAGTCTCAGACGAGCATTTTTGGTTCGGCTGGCCTCAGTTAGTTCTTCATCTGATCCATTTTGTCCTCTTTCAGAATGCATTTGAGATTACATATTTTTTCTGGATATGGTACGAGTTTGGGCTGAGATCTTGTTTCCATGATAATTTCGTTCTCACAGCACTTAGAGTTGCACTTGGGGTAGCAGTTCAGTTTATGTGCAGCTACATCACACTTCCCCTATATGCATTGGTTACGCAGATGGGGTCAACAATGAAGAAGTCGATCTTTGATGAACAAACTAACAAGGCTTTAAAGATATGGCAAAAAAATGCTGCTGCAAAGAAGGCTGGTGGGAAGTCGGTAGGTAAAGCATCTTCGGGTGGCGACTCAGTAACAAAACCACCACCAAAGGCTAAGATGGACAAAAACGCATCAACTCCTGAACAGACAGCAAACATCACGGCCAGCGTAGATATTCAAGGCAACAACACTGACCTCCTATCAGGACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

509

Amino Acids

57.1

Weight (kDa)

9.1

Isoelectric Point (pI)

28.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 10 - 471 5.5e-206 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010739)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42560 AT1G42560 AT2G33670
fragaria_vesca FvH4_5g25860 FvH4_5g25860
malus_domestica MD06G1005800.v1.1 MD10G1233000.v1.1 MD11G1034900.v1.1 MD17G1274600.v1.1
prunus_persica Prupe.5G007100_v2.0.a1
pyrus_communis pycom04g09600 pycom05g07420
rosa_chinensis RchiOBHm_Chr7g0216341
rosa_laevigata RLG00000002580
rosa_roxburghii Rroxscaffold_3G00243310
rosa_rugosa Rorug07G0160000
rosa_samantha Rh7AG297500 Rh7CG317700 Rh7DG299900
rosa_wichuraiana Rw7G025310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 935, 971
AarI CACCTGC 1 cut(s) 311
Acc36I ACCTGC 2 cut(s) 311, 389
AccB7I CCANNNNNTGG 1 cut(s) 648
AciI CCGC 3 cut(s) 5, 66, 416
AclWI GGATC 2 cut(s) 584, 1081
AcoI YGGCCR 2 cut(s) 63, 1476
AcsI RAATTY 2 cut(s) 581, 599
AcyI GRCGYC 1 cut(s) 47
AdeI CACNNNGTG 1 cut(s) 645
AfaI GTAC 2 cut(s) 299, 1145
AfiI CCNNNNNNNGG 1 cut(s) 648
AgsI TTSAA 5 cut(s) 167, 586, 788, 989, 1493
AjnI CCWGG 2 cut(s) 403, 766
AluBI AGCT 9 cut(s) 37, 170, 193, 386, 684, 902, 944, 958, 1236
AluI AGCT 9 cut(s) 37, 170, 193, 386, 684, 902, 944, 958, 1236
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 4 cut(s) 297, 611, 627, 1042
AlwI GGATC 2 cut(s) 584, 1081
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 3 cut(s) 63, 1064, 1476
ApeKI GCWGC 6 cut(s) 272, 408, 958, 1233, 1346, 1349
ApoI RAATTY 2 cut(s) 581, 599
Asp700I GAANNNNTTC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 401, 1520
AsuHPI GGTGA 2 cut(s) 353, 658
AvaII GGWCC 2 cut(s) 401, 1520
BanII GRGCYC 2 cut(s) 39, 61
BarI GAAGNNNNNNTAC 2 cut(s) 1372, 1404
BauI CACGAG 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 6 cut(s) 259, 395, 945, 1245, 1333, 1336
BccI CCATC 5 cut(s) 354, 559, 888, 1267, 1426
BceAI ACGGC 2 cut(s) 53, 1491
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 2 cut(s) 405, 768
BcoDI GTCTC 4 cut(s) 297, 611, 627, 1042
BfmI CTRYAG 2 cut(s) 273, 1008
BfuAI ACCTGC 2 cut(s) 311, 389
BglII AGATCT 1 cut(s) 1160
BisI GCNGC 7 cut(s) 66, 273, 409, 959, 1234, 1347, 1350
BlsI GCNGC 7 cut(s) 67, 274, 410, 960, 1235, 1348, 1351
Bme1390I CCNGG 2 cut(s) 405, 768
Bme18I GGWCC 2 cut(s) 401, 1520
BmgT120I GGNCC 2 cut(s) 401, 1520
BmrFI CCNGG 2 cut(s) 405, 768
BmsI GCATC 2 cut(s) 1394, 1454
BoxI GACNNNNGTC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 194, 234
BsaBI GATNNNNATC 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 47
BsaI GGTCTC 2 cut(s) 297, 627
BsaJI CCNNGG 2 cut(s) 520, 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 648
Bse118I RCCGGY 1 cut(s) 26
Bse8I GATNNNNATC 1 cut(s) 211
BseBI CCWGG 2 cut(s) 405, 768
BseDI CCNNGG 2 cut(s) 520, 1015
BseGI GGATG 1 cut(s) 899
BseJI GATNNNNATC 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 648
BseMII CTCAG 4 cut(s) 1053, 1082, 1148, 1416
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 6 cut(s) 259, 395, 945, 1245, 1333, 1336
BsgI GTGCAG 2 cut(s) 443, 1252
BshFI GGCC 3 cut(s) 65, 1066, 1478
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 648
BsmAI GTCTC 4 cut(s) 297, 611, 627, 1042
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 3 cut(s) 65, 1066, 1478
Bso31I GGTCTC 2 cut(s) 297, 627
Bsp1286I GDGCHC 2 cut(s) 39, 61
Bsp143I GATC 5 cut(s) 40, 589, 1086, 1160, 1296
BspACI CCGC 3 cut(s) 5, 66, 416
BspANI GGCC 3 cut(s) 65, 1066, 1478
BspCNI CTCAG 4 cut(s) 1052, 1081, 1149, 1415
BspMAI CTGCAG 1 cut(s) 277
BspMI ACCTGC 2 cut(s) 311, 389
BspPI GGATC 2 cut(s) 584, 1081
BspTNI GGTCTC 2 cut(s) 297, 627
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 520, 1015
BssMI GATC 5 cut(s) 40, 589, 1086, 1160, 1296
BssNI GRCGYC 1 cut(s) 47
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 2 cut(s) 520, 1015
Bst2BI CACGAG 1 cut(s) 614
Bst2UI CCWGG 2 cut(s) 405, 768
Bst4CI ACNGT 2 cut(s) 503, 1524
BstACI GRCGYC 1 cut(s) 47
BstC8I GCNNGC 5 cut(s) 28, 963, 1005, 1064, 1480
BstDEI CTNAG 7 cut(s) 940, 1039, 1068, 1157, 1196, 1402, 1428
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 899
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 5 cut(s) 43, 592, 1089, 1163, 1299
BstMAI GTCTC 4 cut(s) 297, 611, 627, 1042
BstMBI GATC 5 cut(s) 40, 589, 1086, 1160, 1296
BstMWI GCNNNNNNNGC 2 cut(s) 65, 1358
BstNI CCWGG 2 cut(s) 405, 768
BstNSI RCATGY 1 cut(s) 1007
BstPAI GACNNNNGTC 1 cut(s) 348
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 403, 766
BstSFI CTRYAG 2 cut(s) 273, 1008
BstV1I GCAGC 6 cut(s) 259, 395, 945, 1245, 1333, 1336
BstX2I RGATCY 1 cut(s) 1160
BstXI CCANNNNNNTGG 1 cut(s) 852
BstYI RGATCY 1 cut(s) 1160
BsuRI GGCC 3 cut(s) 65, 1066, 1478
BtgZI GCGATG 1 cut(s) 733
BtsCI GGATG 1 cut(s) 899
BtsIMutI CAGTG 1 cut(s) 1503
BveI ACCTGC 2 cut(s) 311, 389
Cac8I GCNNGC 5 cut(s) 28, 963, 1005, 1064, 1480
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 398
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 401, 1520
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 298, 1144
CviAII CATG 7 cut(s) 54, 121, 733, 740, 890, 1004, 1172
CviQI GTAC 2 cut(s) 298, 1144
DdeI CTNAG 7 cut(s) 940, 1039, 1068, 1157, 1196, 1402, 1428
DpnI GATC 5 cut(s) 42, 591, 1088, 1162, 1298
DpnII GATC 5 cut(s) 40, 589, 1086, 1160, 1296
DraI TTTAAA 2 cut(s) 441, 1326
DraIII CACNNNGTG 1 cut(s) 645
EaeI YGGCCR 2 cut(s) 63, 1476
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 520, 1015
Eco24I GRGCYC 2 cut(s) 39, 61
Eco31I GGTCTC 2 cut(s) 297, 627
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 401, 1520
Eco53kI GAGCTC 1 cut(s) 37
Eco91I GGTNACC 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 403, 766
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 520, 1015
EcoT22I ATGCAT 2 cut(s) 1114, 1261
EcoT38I GRGCYC 2 cut(s) 39, 61
ErhI CCWWGG 2 cut(s) 520, 1015
FaeI CATG 7 cut(s) 57, 124, 736, 743, 893, 1007, 1175
FalI AAGNNNNNCTT 6 cut(s) 177, 209, 767, 799, 1283, 1315
FatI CATG 7 cut(s) 53, 120, 732, 739, 889, 1003, 1171
FauI CCCGC 1 cut(s) 409
Fnu4HI GCNGC 7 cut(s) 66, 273, 409, 959, 1234, 1347, 1350
FokI GGATG 1 cut(s) 906
FriOI GRGCYC 2 cut(s) 39, 61
Fsp4HI GCNGC 7 cut(s) 66, 273, 409, 959, 1234, 1347, 1350
GlaI GCGC 1 cut(s) 397
GluI GCNGC 7 cut(s) 66, 273, 409, 959, 1234, 1347, 1350
HaeIII GGCC 3 cut(s) 65, 1066, 1478
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 7 cut(s) 57, 124, 736, 743, 893, 1007, 1175
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 2 cut(s) 510, 1281
HindII GTYRAC 2 cut(s) 510, 1281
HindIII AAGCTT 1 cut(s) 682
HinfI GANTC 4 cut(s) 535, 837, 865, 1400
HpaI GTTAAC 1 cut(s) 510
HpaII CCGG 1 cut(s) 27
HphI GGTGA 2 cut(s) 353, 658
Hpy166II GTNNAC 3 cut(s) 499, 510, 1281
Hpy188I TCNGA 5 cut(s) 45, 692, 1042, 1086, 1107
Hpy188III TCNNGA 5 cut(s) 173, 614, 1135, 1454, 1518
Hpy8I GTNNAC 3 cut(s) 499, 510, 1281
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 4 cut(s) 145, 541, 679, 1351
HpyCH4III ACNGT 2 cut(s) 503, 1524
HpyCH4IV ACGT 1 cut(s) 492
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 1358
HpyF3I CTNAG 7 cut(s) 940, 1039, 1068, 1157, 1196, 1402, 1428
HpySE526I ACGT 1 cut(s) 492
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 7 cut(s) 57, 124, 736, 743, 893, 1007, 1175
HspAI GCGC 1 cut(s) 396
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 510
Kzo9I GATC 5 cut(s) 40, 589, 1086, 1160, 1296
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 6 cut(s) 259, 395, 945, 1245, 1333, 1336
LweI GCATC 2 cut(s) 1394, 1454
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 3 cut(s) 341, 1264, 1405
MalI GATC 5 cut(s) 42, 591, 1088, 1162, 1298
MboI GATC 5 cut(s) 40, 589, 1086, 1160, 1296
MboII GAAGA 8 cut(s) 107, 326, 457, 537, 824, 1070, 1300, 1380
MflI RGATCY 1 cut(s) 1160
MhlI GDGCHC 2 cut(s) 39, 61
MluCI AATT 6 cut(s) 581, 599, 695, 930, 966, 1177
MlyI GAGTC 2 cut(s) 859, 1394
MmeI TCCRAC 3 cut(s) 106, 629, 928
MnlI CCTC 8 cut(s) 5, 8, 262, 936, 1035, 1077, 1109, 1520
Mph1103I ATGCAT 2 cut(s) 1114, 1261
MroNI GCCGGC 1 cut(s) 26
MroXI GAANNNNTTC 1 cut(s) 111
MseI TTAA 5 cut(s) 440, 509, 530, 822, 1325
MslI CAYNNNNRTG 1 cut(s) 850
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 2 cut(s) 405, 768
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 2 cut(s) 405, 768
MwoI GCNNNNNNNGC 2 cut(s) 65, 1358
NaeI GCCGGC 1 cut(s) 28
NdeII GATC 5 cut(s) 40, 589, 1086, 1160, 1296
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 7 cut(s) 57, 124, 736, 743, 893, 1007, 1175
NmuCI GTSAC 1 cut(s) 341
NsiI ATGCAT 2 cut(s) 1114, 1261
NspI RCATGY 1 cut(s) 1007
PaeI GCATGC 1 cut(s) 1007
PaqCI CACCTGC 1 cut(s) 311
PctI GAATGC 1 cut(s) 1114
PdiI GCCGGC 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 111
PfeI GAWTC 2 cut(s) 535, 837
PflMI CCANNNNNTGG 1 cut(s) 648
PkrI GCNGC 7 cut(s) 67, 274, 410, 960, 1235, 1348, 1351
PleI GAGTC 2 cut(s) 859, 1394
PpsI GAGTC 2 cut(s) 859, 1394
PpuMI RGGWCCY 1 cut(s) 401
PshAI GACNNNNGTC 1 cut(s) 348
PsiI TTATAA 2 cut(s) 935, 971
Psp124BI GAGCTC 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 403, 766
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 403, 766
PspPI GGNCC 2 cut(s) 401, 1520
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 1 cut(s) 277
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 1160
RsaI GTAC 2 cut(s) 299, 1145
RsaNI GTAC 2 cut(s) 298, 1144
RseI CAYNNNNRTG 1 cut(s) 850
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 5 cut(s) 440, 509, 530, 822, 1325
SatI GCNGC 7 cut(s) 66, 273, 409, 959, 1234, 1347, 1350
Sau3AI GATC 5 cut(s) 40, 589, 1086, 1160, 1296
Sau96I GGNCC 2 cut(s) 401, 1520
SchI GAGTC 2 cut(s) 859, 1394
ScrFI CCNGG 2 cut(s) 405, 768
SduI GDGCHC 2 cut(s) 39, 61
SfaNI GCATC 2 cut(s) 1394, 1454
SfcI CTRYAG 2 cut(s) 273, 1008
SinI GGWCC 2 cut(s) 401, 1520
SmiMI CAYNNNNRTG 1 cut(s) 850
SmlI CTYRAG 2 cut(s) 173, 249
SmoI CTYRAG 2 cut(s) 173, 249
SphI GCATGC 1 cut(s) 1007
Sse9I AATT 6 cut(s) 581, 599, 695, 930, 966, 1177
SsiI CCGC 3 cut(s) 5, 66, 416
SspI AATATT 1 cut(s) 486
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 403, 766
StyI CCWWGG 2 cut(s) 520, 1015
TaaI ACNGT 2 cut(s) 503, 1524
TaiI ACGT 1 cut(s) 495
TaqI TCGA 2 cut(s) 39, 1295
TasI AATT 6 cut(s) 581, 599, 695, 930, 966, 1177
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 2 cut(s) 535, 837
Tru1I TTAA 5 cut(s) 440, 509, 530, 822, 1325
Tru9I TTAA 5 cut(s) 440, 509, 530, 822, 1325
TscAI CASTG 1 cut(s) 1510
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 6 cut(s) 272, 408, 958, 1233, 1346, 1349
Tsp45I GTSAC 1 cut(s) 341
TspRI CASTG 1 cut(s) 1510
Van91I CCANNNNNTGG 1 cut(s) 648
VpaK11BI GGWCC 2 cut(s) 401, 1520
XapI RAATTY 2 cut(s) 581, 599
XceI RCATGY 1 cut(s) 1007
XmnI GAANNNNTTC 1 cut(s) 111
Zsp2I ATGCAT 2 cut(s) 1114, 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.