Rroxscaffold_3G00243310

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010739
Physical Location & Seq
Reverse (-)
35846091 .. 35850133
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243310.1

Sequence Viewer

Length: 1344 bp
ATGGCCGGTGGTGGTGGCGCAGGCAGCAGAGAGCTTGATCAGACGCCGACGTGGGCTGTCGCCGGTGTCTGCTTCCTCATCATTCTTATTTCCATAATTTTGGAAAAGGTTCTTCACCTGGTCGGACATGTACAGCTTATGGTTTTGGGATTCATTTCTTTGCTACTGACATTTGGGCAAGCGTACATCGCAAAAAGGTGCATTCCTGAACACTATGCAGATACAATGTTGCCCTGTCCTTACAGAAACGGCCCTGATGATCACGGTGGTGGTGACAAAGGTGGTGACAAAGGTGGTGGAGGAGGAGGTCATCGCAGGCTTTTATGGTATGAGCAGGAGCGCAGAATTTTAGCTGCTGGTAGCTCTGGCAACGCCTGTGCAGCGGGAGAAGTGCCGCTTATCTCTATAAATGGATTACATCAGTTGCACATCTTCATCTTCTTCCTAGCAGTATTTCACGTTATATATGGTGCCATAACCATGACACTAGGAAGATTAAAGATTCGTAGGTGGAAGCAGTGGGAACGAGAAGCTGTAAATGATAGTGAATTCAATGATCCCACGAAATTCAGGCTTACTCACGAGACATCATTTGTGAGAGACCACACAAGATGTTGGACAAAAACACGATTCACCTTCTACTTGGTATGCTTTTTCCGTCAATTCTTTAGGTCTGTTCGTAGGGCTGATTTCTTGACCATGCGGCATGGATTCGTCACTTCCACTGTTATGGACTACGATGACGCTCTATTTGCTTCTCAATGTTCATGGATGGGAGGCTATGTTTGTCCTATAATCTTAGCTGTTGGAACAAAGCTGCAAGGAATTATAACACAAATGGCACTTGAAATCCAAGAAAGACATGCTGTGGTGCAAGGGATACCTCTGGTGCAAGTCACTGATAAGCATTTTTGGTTTAGCTGGCCGGAGCTAGTGCTTTATTTGATTCATTTTGTCCTGTTTCAGTATGAGTTCGGTATCAGGTCTTGTTTCCATGAGAACTTCAATCTTACATTGGTCAGAGTTGGTCTTGGGGTTGCCGTCCAGTTTATGTGCAGCTACATCACACTTCCGCTATATGCATTGGTTACACAGATGGGTTCAACAATGAAGAAGTCAATCTTTGATGAACAAACTAACAAGGCCTTAAAGAAATGGCAAAAGAATGCTCAAAAGAAGAATAAGCATGAGGGCTCGCATAAAACAACTAAACAAAAACTGGGTGGACAGACAGCAAATTCACAGAGTGAAATGGAAACAGAGGGATCAAATCCACGACAATCGGCAAACATCATGGCCAGTGTCGACGTTCAAGGCAAGAATGACCTACTAACAGGACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

447

Amino Acids

50.28

Weight (kDa)

9.06

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 10 - 244 9.3e-78 Mlo family
Mlo PF03094 264 - 418 2.3e-60 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010739)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G42560 AT1G42560 AT2G33670
fragaria_vesca FvH4_5g25860 FvH4_5g25860
malus_domestica MD06G1005800.v1.1 MD10G1233000.v1.1 MD11G1034900.v1.1 MD17G1274600.v1.1
prunus_persica Prupe.5G007100_v2.0.a1
pyrus_communis pycom04g09600 pycom05g07420
rosa_chinensis RchiOBHm_Chr7g0216341
rosa_laevigata RLG00000002580
rosa_roxburghii Rroxscaffold_3G00243310
rosa_rugosa Rorug07G0160000
rosa_samantha Rh7AG297500 Rh7CG317700 Rh7DG299900
rosa_wichuraiana Rw7G025310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AccB1I GGYRCC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 1305
AciI CCGC 4 cut(s) 383, 395, 703, 1073
AclWI GGATC 2 cut(s) 551, 1273
AcoI YGGCCR 3 cut(s) 3, 923, 1296
AcsI RAATTY 4 cut(s) 345, 548, 566, 1237
AcyI GRCGYC 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 1247
AfaI GTAC 2 cut(s) 132, 185
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 5 cut(s) 553, 848, 1006, 1104, 1313
AjiI CACGTC 1 cut(s) 51
AjnI CCWGG 1 cut(s) 117
AleI CACNNNNGTG 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 578, 594
AlwI GGATC 2 cut(s) 551, 1273
AoxI GGCC 5 cut(s) 3, 250, 923, 1143, 1296
ApeKI GCWGC 5 cut(s) 24, 353, 380, 817, 1056
ApoI RAATTY 4 cut(s) 345, 548, 566, 1237
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 2 cut(s) 20, 342
AspS9I GGNCC 2 cut(s) 251, 1337
AsuHPI GGTGA 4 cut(s) 107, 284, 296, 625
AvaII GGWCC 1 cut(s) 1337
BalI TGGCCA 1 cut(s) 1298
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 1196
BauI CACGAG 1 cut(s) 581
BbvI GCAGC 5 cut(s) 36, 340, 392, 804, 1068
BccI CCATC 2 cut(s) 766, 1090
BceAI ACGGC 2 cut(s) 265, 1025
BciT130I CCWGG 1 cut(s) 119
BciVI GTATCC 1 cut(s) 873
BclI TGATCA 2 cut(s) 37, 259
BcoDI GTCTC 2 cut(s) 578, 594
BfaI CTAG 3 cut(s) 446, 488, 932
BfuI GTATCC 1 cut(s) 873
BisI GCNGC 7 cut(s) 25, 354, 381, 395, 704, 818, 1057
BlsI GCNGC 7 cut(s) 26, 355, 382, 396, 705, 819, 1058
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 1337
BmgBI CACGTC 1 cut(s) 51
BmgT120I GGNCC 2 cut(s) 251, 1337
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 119
BmrI ACTGGG 1 cut(s) 1229
BmuI ACTGGG 1 cut(s) 1229
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 594
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bse118I RCCGGY 2 cut(s) 5, 62
Bse1I ACTGG 3 cut(s) 1045, 1224, 1299
BseBI CCWGG 1 cut(s) 119
BseGI GGATG 1 cut(s) 777
BseNI ACTGG 3 cut(s) 1045, 1224, 1299
BseRI GAGGAG 2 cut(s) 315, 318
BseXI GCAGC 5 cut(s) 36, 340, 392, 804, 1068
BsgI GTGCAG 2 cut(s) 399, 1075
BshFI GGCC 5 cut(s) 5, 252, 925, 1145, 1298
BshNI GGYRCC 1 cut(s) 470
BsiSI CCGG 3 cut(s) 6, 63, 926
BsmAI GTCTC 2 cut(s) 578, 594
BsmI GAATGC 2 cut(s) 201, 1171
BsnI GGCC 5 cut(s) 5, 252, 925, 1145, 1298
Bso31I GGTCTC 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 1196
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 4 cut(s) 37, 259, 556, 1265
BspACI CCGC 4 cut(s) 383, 395, 703, 1073
BspANI GGCC 5 cut(s) 5, 252, 925, 1145, 1298
BspLI GGNNCC 1 cut(s) 472
BspPI GGATC 2 cut(s) 551, 1273
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 594
BsrFI RCCGGY 2 cut(s) 5, 62
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 3 cut(s) 1045, 1224, 1299
BssAI RCCGGY 2 cut(s) 5, 62
BssMI GATC 4 cut(s) 37, 259, 556, 1265
BssNI GRCGYC 1 cut(s) 44
BssSI CACGAG 1 cut(s) 581
Bst2BI CACGAG 1 cut(s) 581
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 266, 727, 1341
BstACI GRCGYC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 5 cut(s) 22, 180, 317, 923, 1196
BstDEI CTNAG 1 cut(s) 799
BstF5I GGATG 1 cut(s) 777
BstHHI GCGC 2 cut(s) 20, 342
BstKTI GATC 4 cut(s) 40, 262, 559, 1268
BstMAI GTCTC 2 cut(s) 578, 594
BstMBI GATC 4 cut(s) 37, 259, 556, 1265
BstMWI GCNNNNNNNGC 4 cut(s) 24, 188, 380, 752
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 131, 866
BstSCI CCNGG 1 cut(s) 117
BstV1I GCAGC 5 cut(s) 36, 340, 392, 804, 1068
BstXI CCANNNNNNTGG 2 cut(s) 100, 730
BsuI GTATCC 1 cut(s) 873
BsuRI GGCC 5 cut(s) 5, 252, 925, 1145, 1298
BtgZI GCGATG 2 cut(s) 172, 296
BtrI CACGTC 1 cut(s) 51
BtsCI GGATG 1 cut(s) 777
BtsI GCAGTG 1 cut(s) 524
BtsIMutI CAGTG 4 cut(s) 524, 723, 897, 1306
Cac8I GCNNGC 5 cut(s) 22, 180, 317, 923, 1196
CfoI GCGC 2 cut(s) 20, 342
Cfr10I RCCGGY 2 cut(s) 5, 62
Cfr13I GGNCC 2 cut(s) 251, 1337
CseI GACGC 2 cut(s) 52, 752
CsiI ACCWGGT 1 cut(s) 117
Csp6I GTAC 2 cut(s) 131, 184
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 9 cut(s) 128, 481, 700, 707, 768, 863, 995, 1187, 1294
CviQI GTAC 2 cut(s) 131, 184
DdeI CTNAG 1 cut(s) 799
DpnI GATC 4 cut(s) 39, 261, 558, 1267
DpnII GATC 4 cut(s) 37, 259, 556, 1265
DraIII CACNNNGTG 1 cut(s) 1247
EaeI YGGCCR 3 cut(s) 3, 923, 1296
Eco147I AGGCCT 1 cut(s) 1145
Eco24I GRGCYC 1 cut(s) 1196
Eco31I GGTCTC 1 cut(s) 594
Eco47I GGWCC 1 cut(s) 1337
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 1 cut(s) 1196
FaeI CATG 9 cut(s) 131, 484, 703, 710, 771, 866, 998, 1190, 1297
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 1106, 1138
FatI CATG 9 cut(s) 127, 480, 699, 706, 767, 862, 994, 1186, 1293
FauI CCCGC 1 cut(s) 376
FbaI TGATCA 2 cut(s) 37, 259
FblI GTMKAC 1 cut(s) 1305
Fnu4HI GCNGC 7 cut(s) 25, 354, 381, 395, 704, 818, 1057
FokI GGATG 1 cut(s) 784
FriOI GRGCYC 1 cut(s) 1196
Fsp4HI GCNGC 7 cut(s) 25, 354, 381, 395, 704, 818, 1057
FspBI CTAG 3 cut(s) 446, 488, 932
GlaI GCGC 2 cut(s) 19, 341
GluI GCNGC 7 cut(s) 25, 354, 381, 395, 704, 818, 1057
HaeIII GGCC 5 cut(s) 5, 252, 925, 1145, 1298
HapII CCGG 3 cut(s) 6, 63, 926
HgaI GACGC 2 cut(s) 52, 752
HhaI GCGC 2 cut(s) 20, 342
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 9 cut(s) 131, 484, 703, 710, 771, 866, 998, 1190, 1297
Hin6I GCGC 2 cut(s) 18, 340
HinP1I GCGC 2 cut(s) 18, 340
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HinfI GANTC 5 cut(s) 150, 502, 630, 711, 946
HpaII CCGG 3 cut(s) 6, 63, 926
HphI GGTGA 4 cut(s) 107, 284, 296, 625
Hpy166II GTNNAC 2 cut(s) 1226, 1306
Hpy188I TCNGA 3 cut(s) 42, 125, 1022
Hpy188III TCNNGA 3 cut(s) 206, 581, 694
Hpy8I GTNNAC 2 cut(s) 1226, 1306
Hpy99I CGWCG 2 cut(s) 52, 1310
HpyAV CCTTC 1 cut(s) 646
HpyCH4III ACNGT 3 cut(s) 266, 727, 1341
HpyCH4IV ACGT 3 cut(s) 50, 459, 1308
HpyCH4V TGCA 9 cut(s) 201, 218, 380, 427, 820, 874, 892, 1056, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 188, 380, 752
HpyF3I CTNAG 1 cut(s) 799
HpySE526I ACGT 3 cut(s) 50, 459, 1308
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 9 cut(s) 131, 484, 703, 710, 771, 866, 998, 1190, 1297
HspAI GCGC 2 cut(s) 18, 340
Ksp22I TGATCA 2 cut(s) 37, 259
Kzo9I GATC 4 cut(s) 37, 259, 556, 1265
LmnI GCTCC 2 cut(s) 337, 928
Lsp1109I GCAGC 5 cut(s) 36, 340, 392, 804, 1068
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 3 cut(s) 446, 488, 932
MaeII ACGT 3 cut(s) 50, 459, 1308
MaeIII GTNAC 5 cut(s) 272, 284, 715, 895, 1087
MalI GATC 4 cut(s) 39, 261, 558, 1267
MboI GATC 4 cut(s) 37, 259, 556, 1265
MboII GAAGA 7 cut(s) 104, 424, 430, 433, 504, 1123, 1189
MhlI GDGCHC 1 cut(s) 1196
MlsI TGGCCA 1 cut(s) 1298
MluCI AATT 7 cut(s) 96, 345, 548, 566, 662, 825, 1237
MluNI TGGCCA 1 cut(s) 1298
MmeI TCCRAC 3 cut(s) 103, 596, 787
MnlI CCTC 8 cut(s) 86, 293, 296, 299, 770, 894, 1183, 1255
Mox20I TGGCCA 1 cut(s) 1298
Mph1103I ATGCAT 1 cut(s) 1084
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 1298
MseI TTAA 2 cut(s) 497, 1148
MslI CAYNNNNRTG 3 cut(s) 267, 479, 728
Msp20I TGGCCA 1 cut(s) 1298
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 3 cut(s) 6, 63, 926
MspR9I CCNGG 1 cut(s) 119
Mva1269I GAATGC 2 cut(s) 201, 1171
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 4 cut(s) 24, 188, 380, 752
NdeII GATC 4 cut(s) 37, 259, 556, 1265
NlaIII CATG 9 cut(s) 131, 484, 703, 710, 771, 866, 998, 1190, 1297
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 4 cut(s) 272, 284, 715, 895
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 2 cut(s) 131, 866
OliI CACNNNNGTG 1 cut(s) 267
PceI AGGCCT 1 cut(s) 1145
PciI ACATGT 1 cut(s) 127
PctI GAATGC 2 cut(s) 201, 1171
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 5 cut(s) 150, 502, 630, 711, 946
PkrI GCNGC 7 cut(s) 26, 355, 382, 396, 705, 819, 1058
PscI ACATGT 1 cut(s) 127
PsiI TTATAA 1 cut(s) 830
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 2 cut(s) 251, 1337
RsaI GTAC 2 cut(s) 132, 185
RsaNI GTAC 2 cut(s) 131, 184
RseI CAYNNNNRTG 3 cut(s) 267, 479, 728
SalI GTCGAC 1 cut(s) 1304
SaqAI TTAA 2 cut(s) 497, 1148
SatI GCNGC 7 cut(s) 25, 354, 381, 395, 704, 818, 1057
Sau3AI GATC 4 cut(s) 37, 259, 556, 1265
Sau96I GGNCC 2 cut(s) 251, 1337
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 1196
SexAI ACCWGGT 1 cut(s) 117
SgrAI CRCCGGYG 1 cut(s) 62
SinI GGWCC 1 cut(s) 1337
SmiMI CAYNNNNRTG 3 cut(s) 267, 479, 728
Sse9I AATT 7 cut(s) 96, 345, 548, 566, 662, 825, 1237
SseBI AGGCCT 1 cut(s) 1145
SsiI CCGC 4 cut(s) 383, 395, 703, 1073
SspMI CTAG 3 cut(s) 446, 488, 932
StuI AGGCCT 1 cut(s) 1145
StyD4I CCNGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 266, 727, 1341
TaiI ACGT 3 cut(s) 53, 462, 1311
TaqI TCGA 1 cut(s) 1305
TasI AATT 7 cut(s) 96, 345, 548, 566, 662, 825, 1237
TatI WGTACW 1 cut(s) 130
TauI GCSGC 2 cut(s) 397, 706
TfiI GAWTC 5 cut(s) 150, 502, 630, 711, 946
Tru1I TTAA 2 cut(s) 497, 1148
Tru9I TTAA 2 cut(s) 497, 1148
TscAI CASTG 4 cut(s) 524, 730, 904, 1306
TseFI GTSAC 4 cut(s) 272, 284, 715, 895
TseI GCWGC 5 cut(s) 24, 353, 380, 817, 1056
Tsp45I GTSAC 4 cut(s) 272, 284, 715, 895
TspDTI ATGAA 6 cut(s) 142, 424, 756, 938, 1124, 1143
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 4 cut(s) 524, 730, 904, 1306
VpaK11BI GGWCC 1 cut(s) 1337
XapI RAATTY 4 cut(s) 345, 548, 566, 1237
XceI RCATGY 2 cut(s) 131, 866
XmiI GTMKAC 1 cut(s) 1305
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 3 cut(s) 446, 488, 932
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.