FvH4_5g27090

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008415
Physical Location & Seq
Reverse (-)
18384591 .. 18386092
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27090.t1

Sequence Viewer

Length: 1044 bp
ATGAACGCAATTGATGGTTGCTGGAGATGGAACCCAAACTGGAGAAGCAATCGCCAGCAACTCGCTATGTGTTCGGTAGGATTTGCTGGAAAAATGAGCAACAACATTGGAAAAGATGTTGTAAACTATGAAGTAACAGACCCTAGTGACAATGCGTTAAATCCCCAACCAGGTACTTTGAGATATGGAGCTACTATGATCAAGGACAAAAAATGGATCACTTTCCAGAGGGACATGAAAATCAAACTAGAGAAACCATTGCTAATTAGTAGCTTCACTGCGATTGATGGAAGAGGTGTCAATGTCCACATAGCAGGTAACGCGTGCTTGAGAGTGTTCAAAGCAACCAATGTGATCATCCATGGTCTTCGAATCCACCACTGCAAGTCGCAACCACCAAGTACCGTTATGGGTCCGGACGGAATAGTTTCGCTAGGCGGGGTTGATGGAGACGCAATACGGTTGGTCACTGCATCTAAGGTTTGGATAGACCACAATACACTGTACGAGTGTGAAGATGGTCTTCTTGATGTCACTCGTGGATCAACCAATGTGACAATCTCCAACAACTGGTTCAGAGACCAAGACAAGGTAATGCTTCTCGGCCACGATGATGGCTTCTTTCGGGACAAGAATATGAAGGTGACTGTAGTATATAACCATTTTGGACCACATTGCAACCAAAGAATGCCAAGGATTCGCCATGGATACGCACACGTCGTAAACAACTTGTACAAGGAGTGGACACTGTATGCCATTGGCGGAAGCATGAACCCTAGTGTTAAAAGCGAAGCCAACCTCTTCATTGCATCCCAGCAAAACAAAGAGGTGACTTGGAGAAAGGACATTGTTAGTGACTCCTGGAAATTTTACTCCAAAGGCGATATTTTTGAAAACGGGGCGTCTTTCGTTCAAACAGGTCAAGGTGGAGCCGAGCCGCTCTATAATAAGGAACAGAATTTCCAAGTTGTTGATGCCAAATCTGTCCGGCCATTGACAAGGGCATCAGGTGCTCTAACATGCACCAAACAATCCAGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

348

Amino Acids

38.87

Weight (kDa)

9.18

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 89 - 281 2.1e-27 Pectate lyase
Beta_helix PF13229 110 - 223 3.3e-06 Right handed beta helix region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 305
AccB7I CCANNNNNTGG 1 cut(s) 570
AccBSI CCGCTC 1 cut(s) 940
AccII CGCG 1 cut(s) 323
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 3 cut(s) 438, 762, 938
AclWI GGATC 2 cut(s) 224, 550
AcoI YGGCCR 2 cut(s) 604, 989
AcsI RAATTY 2 cut(s) 866, 958
AcyI GRCGYC 1 cut(s) 902
AfaI GTAC 4 cut(s) 175, 403, 506, 734
AfiI CCNNNNNNNGG 3 cut(s) 170, 570, 589
AflIII ACRYGT 2 cut(s) 321, 715
AgsI TTSAA 3 cut(s) 340, 893, 914
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 2 cut(s) 169, 860
AjuI GAANNNNNNNTTGG 2 cut(s) 557, 589
AloI GAACNNNNNNTCC 2 cut(s) 945, 977
AluBI AGCT 2 cut(s) 191, 273
AluI AGCT 2 cut(s) 191, 273
Alw21I GWGCWC 1 cut(s) 1015
Alw26I GTCTC 2 cut(s) 444, 573
AlwI GGATC 2 cut(s) 224, 550
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 2 cut(s) 604, 989
ApoI RAATTY 2 cut(s) 866, 958
Asp700I GAANNNNTTC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 413, 668
AsuHPI GGTGA 2 cut(s) 655, 841
AsuII TTCGAA 1 cut(s) 370
AvaII GGWCC 2 cut(s) 413, 668
BauI CACGAG 1 cut(s) 537
BbsI GAAGAC 2 cut(s) 359, 515
Bbv12I GWGCWC 1 cut(s) 1015
BccI CCATC 6 cut(s) 8, 21, 281, 440, 512, 608
BciT130I CCWGG 2 cut(s) 171, 862
BciVI GTATCC 1 cut(s) 701
BclI TGATCA 2 cut(s) 198, 354
BcoDI GTCTC 2 cut(s) 444, 573
BfaI CTAG 5 cut(s) 144, 248, 434, 777, 1042
BfmI CTRYAG 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 305
BfuI GTATCC 1 cut(s) 701
BisI GCNGC 1 cut(s) 938
BlsI GCNGC 1 cut(s) 939
Bme1390I CCNGG 2 cut(s) 171, 862
Bme18I GGWCC 2 cut(s) 413, 668
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 413, 668
BmiI GGNNCC 3 cut(s) 32, 414, 931
BmrFI CCNGG 2 cut(s) 171, 862
BmsI GCATC 5 cut(s) 482, 818, 964, 1013, 1028
BpiI GAAGAC 2 cut(s) 359, 515
BpmI CTGGAG 2 cut(s) 43, 61
Bpu14I TTCGAA 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 902
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 361, 692, 703
BsaWI WCCGGW 1 cut(s) 415
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 570, 589
Bse1I ACTGG 2 cut(s) 44, 575
Bse3DI GCAATG 3 cut(s) 257, 673, 804
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 2 cut(s) 171, 862
BseDI CCNNGG 3 cut(s) 361, 692, 703
BseGI GGATG 2 cut(s) 357, 809
BseLI CCNNNNNNNGG 3 cut(s) 170, 570, 589
BseMI GCAATG 3 cut(s) 257, 673, 804
BseNI ACTGG 2 cut(s) 44, 575
BseYI CCCAGC 1 cut(s) 813
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 2 cut(s) 606, 991
BsiHKAI GWGCWC 1 cut(s) 1015
BsiSI CCGG 2 cut(s) 416, 988
BslFI GGGAC 2 cut(s) 245, 641
BslI CCNNNNNNNGG 3 cut(s) 170, 570, 589
BsmAI GTCTC 2 cut(s) 444, 573
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 245, 641
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 2 cut(s) 606, 991
Bso31I GGTCTC 1 cut(s) 573
Bsp119I TTCGAA 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 1015
Bsp13I TCCGGA 1 cut(s) 415
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 198, 216, 354, 542
Bsp19I CCATGG 2 cut(s) 361, 703
BspACI CCGC 3 cut(s) 438, 762, 938
BspANI GGCC 2 cut(s) 606, 991
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 3 cut(s) 32, 414, 931
BspMI ACCTGC 1 cut(s) 305
BspPI GGATC 2 cut(s) 224, 550
BspT104I TTCGAA 1 cut(s) 370
BspTNI GGTCTC 1 cut(s) 573
BsrBI CCGCTC 1 cut(s) 940
BsrDI GCAATG 3 cut(s) 257, 673, 804
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 2 cut(s) 44, 575
BssECI CCNNGG 3 cut(s) 361, 692, 703
BssMI GATC 4 cut(s) 198, 216, 354, 542
BssNI GRCGYC 1 cut(s) 902
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 3 cut(s) 361, 692, 703
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 2 cut(s) 171, 862
Bst4CI ACNGT 5 cut(s) 406, 462, 504, 649, 750
Bst6I CTCTTC 2 cut(s) 286, 806
BstACI GRCGYC 1 cut(s) 902
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstAUI TGTACA 1 cut(s) 732
BstBI TTCGAA 1 cut(s) 370
BstC8I GCNNGC 2 cut(s) 56, 325
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 2 cut(s) 361, 703
BstF5I GGATG 2 cut(s) 357, 809
BstFNI CGCG 1 cut(s) 323
BstKTI GATC 4 cut(s) 201, 219, 357, 545
BstMAI GTCTC 2 cut(s) 444, 573
BstMBI GATC 4 cut(s) 198, 216, 354, 542
BstMWI GCNNNNNNNGC 2 cut(s) 320, 1010
BstNI CCWGG 2 cut(s) 171, 862
BstNSI RCATGY 1 cut(s) 1023
BstSCI CCNGG 2 cut(s) 169, 860
BstSFI CTRYAG 1 cut(s) 648
BstUI CGCG 1 cut(s) 323
BstV2I GAAGAC 2 cut(s) 359, 515
BstXI CCANNNNNNTGG 1 cut(s) 614
BsuI GTATCC 1 cut(s) 701
BsuRI GGCC 2 cut(s) 606, 991
BtgI CCRYGG 2 cut(s) 361, 703
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 2 cut(s) 357, 809
BtsI GCAGTG 3 cut(s) 276, 379, 468
BtsIMutI CAGTG 5 cut(s) 276, 379, 468, 500, 746
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 2 cut(s) 56, 325
Cfr13I GGNCC 2 cut(s) 413, 668
CseI GACGC 2 cut(s) 461, 891
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 4 cut(s) 174, 402, 505, 733
CviAII CATG 5 cut(s) 235, 362, 704, 769, 1020
CviJI RGCY 8 cut(s) 191, 273, 606, 618, 794, 932, 937, 991
CviKI_1 RGCY 8 cut(s) 191, 273, 606, 618, 794, 932, 937, 991
CviQI GTAC 4 cut(s) 174, 402, 505, 733
DdeI CTNAG 1 cut(s) 477
DpnI GATC 4 cut(s) 200, 218, 356, 544
DpnII GATC 4 cut(s) 198, 216, 354, 542
EaeI YGGCCR 2 cut(s) 604, 989
Eam1104I CTCTTC 2 cut(s) 286, 806
EarI CTCTTC 2 cut(s) 286, 806
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 3 cut(s) 361, 692, 703
Eco31I GGTCTC 1 cut(s) 573
Eco47I GGWCC 2 cut(s) 413, 668
EcoRII CCWGG 2 cut(s) 169, 860
EcoT14I CCWWGG 3 cut(s) 361, 692, 703
ErhI CCWWGG 3 cut(s) 361, 692, 703
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 5 cut(s) 238, 365, 707, 772, 1023
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 245, 641
FatI CATG 5 cut(s) 234, 361, 703, 768, 1019
FauI CCCGC 1 cut(s) 431
FbaI TGATCA 2 cut(s) 198, 354
Fnu4HI GCNGC 1 cut(s) 938
FokI GGATG 2 cut(s) 344, 796
Fsp4HI GCNGC 1 cut(s) 938
FspBI CTAG 5 cut(s) 144, 248, 434, 777, 1042
GluI GCNGC 1 cut(s) 938
GsaI CCCAGC 1 cut(s) 817
GsuI CTGGAG 2 cut(s) 43, 61
HaeIII GGCC 2 cut(s) 606, 991
HapII CCGG 2 cut(s) 416, 988
HgaI GACGC 2 cut(s) 461, 891
Hin1I GRCGYC 1 cut(s) 902
Hin1II CATG 5 cut(s) 238, 365, 707, 772, 1023
HinfI GANTC 3 cut(s) 372, 697, 857
HpaII CCGG 2 cut(s) 416, 988
HphI GGTGA 2 cut(s) 655, 841
Hpy166II GTNNAC 4 cut(s) 124, 307, 724, 744
Hpy188I TCNGA 1 cut(s) 578
Hpy188III TCNNGA 5 cut(s) 226, 416, 527, 626, 1035
Hpy8I GTNNAC 4 cut(s) 124, 307, 724, 744
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 1 cut(s) 634
HpyCH4III ACNGT 5 cut(s) 406, 462, 504, 649, 750
HpyCH4IV ACGT 1 cut(s) 717
HpyCH4V TGCA 5 cut(s) 384, 473, 678, 809, 1023
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 1010
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 1 cut(s) 717
Hsp92I GRCGYC 1 cut(s) 902
Hsp92II CATG 5 cut(s) 238, 365, 707, 772, 1023
Kpn2I TCCGGA 1 cut(s) 415
Ksp22I TGATCA 2 cut(s) 198, 354
Kzo9I GATC 4 cut(s) 198, 216, 354, 542
LmnI GCTCC 2 cut(s) 188, 929
LweI GCATC 5 cut(s) 482, 818, 964, 1013, 1028
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 5 cut(s) 144, 248, 434, 777, 1042
MaeII ACGT 1 cut(s) 717
MaeIII GTNAC 9 cut(s) 133, 146, 317, 466, 532, 553, 643, 829, 854
MalI GATC 4 cut(s) 200, 218, 356, 544
MbiI CCGCTC 1 cut(s) 940
MboI GATC 4 cut(s) 198, 216, 354, 542
MboII GAAGA 5 cut(s) 303, 359, 515, 527, 793
MfeI CAATTG 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 1015
MluCI AATT 4 cut(s) 9, 264, 866, 958
MluI ACGCGT 1 cut(s) 321
MlyI GAGTC 1 cut(s) 851
MmeI TCCRAC 1 cut(s) 588
MnlI CCTC 4 cut(s) 222, 287, 809, 820
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 2 cut(s) 158, 783
MslI CAYNNNNRTG 1 cut(s) 612
MspI CCGG 2 cut(s) 416, 988
MspR9I CCNGG 2 cut(s) 171, 862
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 2 cut(s) 171, 862
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 320, 1010
NcoI CCATGG 2 cut(s) 361, 703
NdeII GATC 4 cut(s) 198, 216, 354, 542
NlaIII CATG 5 cut(s) 238, 365, 707, 772, 1023
NlaIV GGNNCC 3 cut(s) 32, 414, 931
NmeAIII GCCGAG 2 cut(s) 582, 958
NmuCI GTSAC 7 cut(s) 146, 466, 532, 553, 643, 829, 854
NspI RCATGY 1 cut(s) 1023
NspV TTCGAA 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PctI GAATGC 1 cut(s) 693
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 2 cut(s) 372, 697
PflMI CCANNNNNTGG 1 cut(s) 570
PfoI TCCNGGA 1 cut(s) 860
PkrI GCNGC 1 cut(s) 939
PleI GAGTC 1 cut(s) 851
PpsI GAGTC 1 cut(s) 851
Psp6I CCWGG 2 cut(s) 169, 860
PspFI CCCAGC 1 cut(s) 813
PspGI CCWGG 2 cut(s) 169, 860
PspN4I GGNNCC 3 cut(s) 32, 414, 931
PspPI GGNCC 2 cut(s) 413, 668
RsaI GTAC 4 cut(s) 175, 403, 506, 734
RsaNI GTAC 4 cut(s) 174, 402, 505, 733
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 2 cut(s) 158, 783
SatI GCNGC 1 cut(s) 938
Sau3AI GATC 4 cut(s) 198, 216, 354, 542
Sau96I GGNCC 2 cut(s) 413, 668
SchI GAGTC 1 cut(s) 851
ScrFI CCNGG 2 cut(s) 171, 862
SduI GDGCHC 1 cut(s) 1015
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 5 cut(s) 482, 818, 964, 1013, 1028
SfcI CTRYAG 1 cut(s) 648
SfuI TTCGAA 1 cut(s) 370
SinI GGWCC 2 cut(s) 413, 668
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
Sse9I AATT 4 cut(s) 9, 264, 866, 958
SsiI CCGC 3 cut(s) 438, 762, 938
SspMI CTAG 5 cut(s) 144, 248, 434, 777, 1042
StyD4I CCNGG 2 cut(s) 169, 860
StyI CCWWGG 3 cut(s) 361, 692, 703
TaaI ACNGT 5 cut(s) 406, 462, 504, 649, 750
TaiI ACGT 1 cut(s) 720
TaqI TCGA 1 cut(s) 370
TasI AATT 4 cut(s) 9, 264, 866, 958
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 2 cut(s) 372, 697
Tru1I TTAA 2 cut(s) 158, 783
Tru9I TTAA 2 cut(s) 158, 783
TscAI CASTG 5 cut(s) 283, 386, 475, 507, 753
TseFI GTSAC 7 cut(s) 146, 466, 532, 553, 643, 829, 854
Tsp45I GTSAC 7 cut(s) 146, 466, 532, 553, 643, 829, 854
TspDTI ATGAA 6 cut(s) 17, 144, 251, 653, 785, 793
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 5 cut(s) 283, 386, 475, 507, 753
Van91I CCANNNNNTGG 1 cut(s) 570
VpaK11BI GGWCC 2 cut(s) 413, 668
XapI RAATTY 2 cut(s) 866, 958
XceI RCATGY 1 cut(s) 1023
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 5 cut(s) 144, 248, 434, 777, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.