Rh7DG315200

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008415
Physical Location & Seq
Reverse (-)
37022700 .. 37023679
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG315200.1

Sequence Viewer

Length: 846 bp
ATGGCTTCTCAATACTGCTTTGTTTTGGTTAATGTCATCATTCTCCTCTGCAGCTTGGGTCCGAACCTGAGTTTGGCAAAGGAAACAAAAGTCCATGGCTTGAAAATGAACGCAATTGACTGTTGCTGGAGATGGAATCCAAATTGGAGAAGCAACCGCCAGAACCTCGCAATGTGTTCGGTAGGATTTGCCGGAAAAATGAGCAACAACATAGGAAAAGATGTCATAAACTATGAAGTAACAGATCCCAGTGACAATGCATTAAATCCCCAACCAGGCACTTTGAGATATGGAGCTACTATGATCAAAGGCAAAAAATGGATCACATTCCAGAGGGACATGAAAATTAAACTCGAGAAACCATTGCTCGTTAGTAGCTTCACTGCAATTGATGGAAGAGGTGCTAATGTCCACATAAAAGGAAACGCGTGCTTGAGAGTGTTCAAAGCAACCAATGTGATAATACATGGCCTTCGAATCCACCACTGCAAGTCGCAGCCACCGACCACGGTTATGGGTCCGAATGGAATAGTTTCACTGGGAGCGGTTGATGGAGACGCAATAAGGTTGGTTACTGCATCAAAGGTTTGGATAGACCACAATACACTATACGAGTGCGAGGATGGTCTTCTCGACGTCACTCGTGGATCAACCGATGTCACCGTCTCCAACAACTGGTTCAGAAACCAGGACAAGGTAATGCTTCTCGGCCATGATGATGGCTTCCTTCGGGACAAGAACATGAAGGTGACTGTAGTGTACAATCATTTTGGACCACTCTGCAATCAGAGAATGCCAAGGTACGTAACCACTGCTTTGCTCAGTTATCCAGACTATTTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

281

Amino Acids

31.33

Weight (kDa)

9.19

Isoelectric Point (pI)

23.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 123 - 269 3.4e-15 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 639
AccB7I CCANNNNNTGG 1 cut(s) 675
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 157, 545
AclWI GGATC 3 cut(s) 239, 329, 655
AcoI YGGCCR 1 cut(s) 709
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 2 cut(s) 761, 803
AfiI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
AflIII ACRYGT 1 cut(s) 426
AgsI TTSAA 2 cut(s) 103, 445
AjnI CCWGG 2 cut(s) 274, 687
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 662, 694
AluBI AGCT 3 cut(s) 54, 296, 378
AluI AGCT 3 cut(s) 54, 296, 378
Alw26I GTCTC 2 cut(s) 549, 670
AlwI GGATC 3 cut(s) 239, 329, 655
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 2 cut(s) 469, 709
ApeKI GCWGC 2 cut(s) 51, 496
Asp700I GAANNNNTTC 1 cut(s) 532
AspS9I GGNCC 3 cut(s) 59, 518, 773
AsuHPI GGTGA 2 cut(s) 652, 760
AsuII TTCGAA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 3 cut(s) 59, 518, 773
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 620
BbvI GCAGC 2 cut(s) 63, 508
BccI CCATC 5 cut(s) 126, 386, 545, 617, 713
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BciT130I CCWGG 2 cut(s) 276, 689
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 2 cut(s) 549, 670
BfaI CTAG 1 cut(s) 844
BfmI CTRYAG 2 cut(s) 49, 753
BisI GCNGC 2 cut(s) 52, 497
BlsI GCNGC 2 cut(s) 53, 498
Bme1390I CCNGG 2 cut(s) 276, 689
Bme18I GGWCC 3 cut(s) 59, 518, 773
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 3 cut(s) 59, 518, 773
BmiI GGNNCC 2 cut(s) 60, 519
BmrFI CCNGG 2 cut(s) 276, 689
BmrI ACTGGG 2 cut(s) 243, 548
BmsI GCATC 1 cut(s) 587
BmuI ACTGGG 2 cut(s) 243, 548
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 805
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 94, 507, 797
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
Bse1I ACTGG 3 cut(s) 249, 543, 680
Bse3DI GCAATG 2 cut(s) 177, 362
BseBI CCWGG 2 cut(s) 276, 689
BseDI CCNNGG 3 cut(s) 94, 507, 797
BseGI GGATG 1 cut(s) 628
BseLI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
BseMI GCAATG 2 cut(s) 177, 362
BseMII CTCAG 2 cut(s) 59, 835
BseNI ACTGG 3 cut(s) 249, 543, 680
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 63, 508
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 2 cut(s) 471, 711
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 350, 746
BslI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
BsmAI GTCTC 2 cut(s) 549, 670
BsmBI CGTCTC 2 cut(s) 549, 670
BsmFI GGGAC 2 cut(s) 350, 746
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 2 cut(s) 471, 711
BsoBI CYCGRG 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 759
Bsp143I GATC 4 cut(s) 244, 303, 321, 647
Bsp19I CCATGG 1 cut(s) 94
BspACI CCGC 2 cut(s) 157, 545
BspANI GGCC 2 cut(s) 471, 711
BspCNI CTCAG 2 cut(s) 60, 834
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 2 cut(s) 60, 519
BspMAI CTGCAG 1 cut(s) 53
BspPI GGATC 3 cut(s) 239, 329, 655
BspT104I TTCGAA 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 545
BsrDI GCAATG 2 cut(s) 177, 362
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 3 cut(s) 249, 543, 680
BssECI CCNNGG 3 cut(s) 94, 507, 797
BssMI GATC 4 cut(s) 244, 303, 321, 647
BssNI GRCGYC 1 cut(s) 636
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 2 cut(s) 94, 797
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 2 cut(s) 276, 689
Bst4CI ACNGT 4 cut(s) 122, 511, 664, 754
Bst6I CTCTTC 1 cut(s) 391
BstACI GRCGYC 1 cut(s) 636
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 805
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 2 cut(s) 68, 821
BstDSI CCRYGG 2 cut(s) 94, 507
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 4 cut(s) 247, 306, 324, 650
BstMAI GTCTC 2 cut(s) 549, 670
BstMBI GATC 4 cut(s) 244, 303, 321, 647
BstNI CCWGG 2 cut(s) 276, 689
BstSCI CCNGG 2 cut(s) 274, 687
BstSFI CTRYAG 2 cut(s) 49, 753
BstSNI TACGTA 1 cut(s) 805
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 63, 508
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 244
BstXI CCANNNNNNTGG 2 cut(s) 514, 719
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 471, 711
BtgI CCRYGG 2 cut(s) 94, 507
BtsCI GGATG 1 cut(s) 628
BtsI GCAGTG 3 cut(s) 381, 484, 810
BtsIMutI CAGTG 5 cut(s) 256, 381, 484, 536, 810
Cac8I GCNNGC 1 cut(s) 430
Cfr13I GGNCC 3 cut(s) 59, 518, 773
CseI GACGC 1 cut(s) 566
Csp6I GTAC 2 cut(s) 760, 802
CviAII CATG 5 cut(s) 95, 340, 467, 713, 742
CviQI GTAC 2 cut(s) 760, 802
DdeI CTNAG 2 cut(s) 68, 821
DpnI GATC 4 cut(s) 246, 305, 323, 649
DpnII GATC 4 cut(s) 244, 303, 321, 647
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Eco105I TACGTA 1 cut(s) 805
Eco130I CCWWGG 2 cut(s) 94, 797
Eco47I GGWCC 3 cut(s) 59, 518, 773
Eco88I CYCGRG 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 274, 687
EcoT14I CCWWGG 2 cut(s) 94, 797
EcoT22I ATGCAT 1 cut(s) 262
ErhI CCWWGG 2 cut(s) 94, 797
Esp3I CGTCTC 2 cut(s) 549, 670
FaeI CATG 5 cut(s) 98, 343, 470, 716, 745
FaqI GGGAC 2 cut(s) 350, 746
FatI CATG 5 cut(s) 94, 339, 466, 712, 741
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 2 cut(s) 52, 497
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 2 cut(s) 52, 497
FspBI CTAG 1 cut(s) 844
GluI GCNGC 2 cut(s) 52, 497
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 2 cut(s) 471, 711
HapII CCGG 1 cut(s) 192
HgaI GACGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 5 cut(s) 98, 343, 470, 716, 745
HinfI GANTC 2 cut(s) 136, 477
HpaII CCGG 1 cut(s) 192
HphI GGTGA 2 cut(s) 652, 760
Hpy166II GTNNAC 2 cut(s) 412, 760
Hpy188I TCNGA 4 cut(s) 63, 522, 683, 789
Hpy188III TCNNGA 5 cut(s) 331, 355, 632, 731, 830
Hpy8I GTNNAC 2 cut(s) 412, 760
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 482, 737, 739
HpyCH4III ACNGT 4 cut(s) 122, 511, 664, 754
HpyCH4IV ACGT 2 cut(s) 636, 804
HpyCH4V TGCA 6 cut(s) 51, 260, 386, 489, 578, 783
HpyF3I CTNAG 2 cut(s) 68, 821
HpySE526I ACGT 2 cut(s) 636, 804
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 5 cut(s) 98, 343, 470, 716, 745
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 4 cut(s) 244, 303, 321, 647
LmnI GCTCC 2 cut(s) 293, 542
Lsp1109I GCAGC 2 cut(s) 63, 508
LweI GCATC 1 cut(s) 587
MaeI CTAG 1 cut(s) 844
MaeII ACGT 2 cut(s) 636, 804
MaeIII GTNAC 7 cut(s) 238, 251, 571, 637, 658, 748, 805
MalI GATC 4 cut(s) 246, 305, 323, 649
MbiI CCGCTC 1 cut(s) 545
MboI GATC 4 cut(s) 244, 303, 321, 647
MboII GAAGA 2 cut(s) 408, 620
MfeI CAATTG 2 cut(s) 114, 387
MflI RGATCY 1 cut(s) 244
MluCI AATT 4 cut(s) 114, 142, 345, 387
MluI ACGCGT 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 693
MnlI CCTC 5 cut(s) 56, 176, 327, 392, 613
Mph1103I ATGCAT 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 532
MseI TTAA 3 cut(s) 30, 263, 348
MslI CAYNNNNRTG 3 cut(s) 512, 717, 746
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 276, 689
MunI CAATTG 2 cut(s) 114, 387
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 2 cut(s) 276, 689
MvnI CGCG 1 cut(s) 428
NcoI CCATGG 1 cut(s) 94
NdeII GATC 4 cut(s) 244, 303, 321, 647
NlaIII CATG 5 cut(s) 98, 343, 470, 716, 745
NlaIV GGNNCC 2 cut(s) 60, 519
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 4 cut(s) 251, 637, 658, 748
NsiI ATGCAT 1 cut(s) 262
NspV TTCGAA 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PctI GAATGC 1 cut(s) 798
PdmI GAANNNNTTC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 136, 477
PflMI CCANNNNNTGG 1 cut(s) 675
PkrI GCNGC 2 cut(s) 53, 498
Ppu21I YACGTR 1 cut(s) 805
Psp6I CCWGG 2 cut(s) 274, 687
PspGI CCWGG 2 cut(s) 274, 687
PspN4I GGNNCC 2 cut(s) 60, 519
PspPI GGNCC 3 cut(s) 59, 518, 773
PstI CTGCAG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 2 cut(s) 761, 803
RsaNI GTAC 2 cut(s) 760, 802
RseI CAYNNNNRTG 3 cut(s) 512, 717, 746
SaqAI TTAA 3 cut(s) 30, 263, 348
SatI GCNGC 2 cut(s) 52, 497
Sau3AI GATC 4 cut(s) 244, 303, 321, 647
Sau96I GGNCC 3 cut(s) 59, 518, 773
ScrFI CCNGG 2 cut(s) 276, 689
SfaNI GCATC 1 cut(s) 587
SfcI CTRYAG 2 cut(s) 49, 753
Sfr274I CTCGAG 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 3 cut(s) 59, 518, 773
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 3 cut(s) 512, 717, 746
SmlI CTYRAG 2 cut(s) 353, 433
SmoI CTYRAG 2 cut(s) 353, 433
SnaBI TACGTA 1 cut(s) 805
Sse9I AATT 4 cut(s) 114, 142, 345, 387
SsiI CCGC 2 cut(s) 157, 545
SspMI CTAG 1 cut(s) 844
StyD4I CCNGG 2 cut(s) 274, 687
StyI CCWWGG 2 cut(s) 94, 797
TaaI ACNGT 4 cut(s) 122, 511, 664, 754
TaiI ACGT 2 cut(s) 639, 807
TaqI TCGA 3 cut(s) 354, 475, 633
TasI AATT 4 cut(s) 114, 142, 345, 387
TatI WGTACW 1 cut(s) 759
TfiI GAWTC 2 cut(s) 136, 477
Tru1I TTAA 3 cut(s) 30, 263, 348
Tru9I TTAA 3 cut(s) 30, 263, 348
TscAI CASTG 5 cut(s) 256, 388, 491, 543, 817
TseFI GTSAC 4 cut(s) 251, 637, 658, 748
TseI GCWGC 2 cut(s) 51, 496
Tsp45I GTSAC 4 cut(s) 251, 637, 658, 748
TspDTI ATGAA 4 cut(s) 122, 249, 356, 758
TspRI CASTG 5 cut(s) 256, 388, 491, 543, 817
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 3 cut(s) 59, 518, 773
XhoI CTCGAG 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 532
XspI CTAG 1 cut(s) 844
ZraI GACGTC 1 cut(s) 637
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.