Prupe.5G245600_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0008415
Physical Location & Seq
Reverse (-)
18381205 .. 18382420
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G245600.1

Sequence Viewer

Length: 735 bp
GGGGCTACCATCATCAGAGGGAAAAAGTGGATCACCTTCCAGAGGGACATGCGCATTAGGCTTGAGAAACCACTTCTCATTAGTAGCTTCACTGCCATTGACGGAAGAGGTCCTAGCGTTCACATTGCTGGTAATGCATGCTTGGTGGTCTTTAAGGCAAGCAATATAATCATCCATGGCCTTCGAATCCACCACTGCCGTTCCCAAGCACCAAGCTTGGTTATGGGTCCAGATGGAAAGACAATGCCACTGGGGCAGGTTGATGGAGATGCAATCAGGTTGGTTACTGCATCAAAAGATGGTCTTCTCGATGTTACTCGTGGATCTACCGATATCACCGTCTCCAACAATTGGTTCAGAGACCAAGACAAGGTCATGCTTCTGGGCCACGATGATGGCTATTTTCGGGATAAGAACATGAGGGTGACCGTAGTGTACAACCATTTTGGACCTAACTGCAACCAAAGAATGCCAAGGATTCGTTACGGATATGCACATGTCGTGAACAATCTCTACAGAGAATGGTCTCAGTATGCCATTGGGGGAGGAAGCATGAACCCTAGCGTTAAGAGTGAAGCCAACCTCTTCATTGCACCAAAATCGAGAAACAATAAAGAGATCACTTGGAGGAAGGACAGCATTGGAAACAAAGAATCCTGGAAGTTTTACTCGGTGGGAGATATCTTTGAAAATAGGGCTTCTTTCATCAGAGCAACTCCACCCATTTGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

245

Amino Acids

27.44

Weight (kDa)

9.82

Isoelectric Point (pI)

27.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 53
Acc36I ACCTGC 1 cut(s) 247
AccB7I CCANNNNNTGG 1 cut(s) 351
AclWI GGATC 2 cut(s) 38, 331
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 3 cut(s) 42, 351, 370
AflIII ACRYGT 1 cut(s) 496
AgsI TTSAA 1 cut(s) 689
AjnI CCWGG 1 cut(s) 656
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 2 cut(s) 87, 216
AluI AGCT 2 cut(s) 87, 216
Alw26I GTCTC 3 cut(s) 346, 354, 531
AlwI GGATC 2 cut(s) 38, 331
AoxI GGCC 2 cut(s) 178, 385
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 4 cut(s) 110, 227, 385, 449
AsuHPI GGTGA 3 cut(s) 25, 328, 436
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 3 cut(s) 110, 227, 449
BauI CACGAG 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 296
BccI CCATC 5 cut(s) 17, 227, 257, 293, 389
BceAI ACGGC 1 cut(s) 183
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciT130I CCWGG 1 cut(s) 658
BcoDI GTCTC 3 cut(s) 346, 354, 531
BfaI CTAG 2 cut(s) 114, 561
BfmI CTRYAG 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 247
BglI GCCNNNNNGGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 658
Bme18I GGWCC 3 cut(s) 110, 227, 449
BmgT120I GGNCC 4 cut(s) 110, 227, 385, 449
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 658
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 259, 299
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 83
BsaI GGTCTC 2 cut(s) 354, 531
BsaJI CCNNGG 2 cut(s) 175, 473
Bsc4I CCNNNNNNNGG 3 cut(s) 42, 351, 370
Bse1I ACTGG 1 cut(s) 255
Bse3DI GCAATG 2 cut(s) 123, 588
BseBI CCWGG 1 cut(s) 658
BseDI CCNNGG 2 cut(s) 175, 473
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 42, 351, 370
BseMI GCAATG 2 cut(s) 123, 588
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 1 cut(s) 255
BshFI GGCC 2 cut(s) 180, 387
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 3 cut(s) 42, 351, 370
BsmAI GTCTC 3 cut(s) 346, 354, 531
BsmBI CGTCTC 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 474
BsnI GGCC 2 cut(s) 180, 387
Bso31I GGTCTC 2 cut(s) 354, 531
Bsp119I TTCGAA 1 cut(s) 184
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 30, 323, 618
Bsp19I CCATGG 1 cut(s) 175
BspANI GGCC 2 cut(s) 180, 387
BspCNI CTCAG 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 2 cut(s) 38, 331
BspT104I TTCGAA 1 cut(s) 184
BspTNI GGTCTC 2 cut(s) 354, 531
BsrDI GCAATG 2 cut(s) 123, 588
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 175, 473
BssMI GATC 3 cut(s) 30, 323, 618
BssSI CACGAG 1 cut(s) 318
BssT1I CCWWGG 2 cut(s) 175, 473
Bst2BI CACGAG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 658
Bst4CI ACNGT 2 cut(s) 340, 430
Bst6I CTCTTC 2 cut(s) 100, 590
BstAUI TGTACA 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 139, 160
BstDEI CTNAG 2 cut(s) 528, 732
BstDSI CCRYGG 1 cut(s) 175
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 40
BstF5I GGATG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 3 cut(s) 33, 326, 621
BstMAI GTCTC 3 cut(s) 346, 354, 531
BstMBI GATC 3 cut(s) 30, 323, 618
BstMWI GCNNNNNNNGC 3 cut(s) 58, 134, 253
BstNI CCWGG 1 cut(s) 658
BstNSI RCATGY 3 cut(s) 52, 141, 500
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 656
BstSFI CTRYAG 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 323
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 1 cut(s) 323
BsuRI GGCC 2 cut(s) 180, 387
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 1 cut(s) 171
BtsI GCAGTG 2 cut(s) 90, 193
BtsIMutI CAGTG 3 cut(s) 90, 193, 248
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 139, 160
CfoI GCGC 1 cut(s) 54
Cfr13I GGNCC 4 cut(s) 110, 227, 385, 449
Csp6I GTAC 1 cut(s) 436
CviAII CATG 7 cut(s) 49, 138, 176, 376, 418, 497, 553
CviJI RGCY 9 cut(s) 5, 61, 87, 180, 216, 387, 399, 578, 698
CviKI_1 RGCY 9 cut(s) 5, 61, 87, 180, 216, 387, 399, 578, 698
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 2 cut(s) 528, 732
DpnI GATC 3 cut(s) 32, 325, 620
DpnII GATC 3 cut(s) 30, 323, 618
Eam1104I CTCTTC 2 cut(s) 100, 590
EarI CTCTTC 2 cut(s) 100, 590
Eco130I CCWWGG 2 cut(s) 175, 473
Eco31I GGTCTC 2 cut(s) 354, 531
Eco32I GATATC 2 cut(s) 334, 682
Eco47I GGWCC 3 cut(s) 110, 227, 449
Eco91I GGTNACC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 110
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 656
EcoRV GATATC 2 cut(s) 334, 682
EcoT14I CCWWGG 2 cut(s) 175, 473
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 2 cut(s) 175, 473
Esp3I CGTCTC 1 cut(s) 346
FaeI CATG 7 cut(s) 52, 141, 179, 379, 421, 500, 556
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 1 cut(s) 59
FatI CATG 7 cut(s) 48, 137, 175, 375, 417, 496, 552
FokI GGATG 1 cut(s) 158
FspAI RTGCGCAY 1 cut(s) 53
FspBI CTAG 2 cut(s) 114, 561
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 1 cut(s) 53
HaeIII GGCC 2 cut(s) 180, 387
HhaI GCGC 1 cut(s) 54
Hin1II CATG 7 cut(s) 52, 141, 179, 379, 421, 500, 556
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 3 cut(s) 186, 478, 653
HphI GGTGA 3 cut(s) 25, 328, 436
Hpy166II GTNNAC 3 cut(s) 121, 436, 505
Hpy188I TCNGA 3 cut(s) 17, 359, 710
Hpy188III TCNNGA 6 cut(s) 40, 230, 308, 407, 502, 603
Hpy8I GTNNAC 3 cut(s) 121, 436, 505
HpyAV CCTTC 3 cut(s) 46, 191, 625
HpyCH4III ACNGT 2 cut(s) 340, 430
HpyCH4V TGCA 6 cut(s) 137, 272, 290, 459, 494, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 134, 253
HpyF3I CTNAG 2 cut(s) 528, 732
Hsp92II CATG 7 cut(s) 52, 141, 179, 379, 421, 500, 556
HspAI GCGC 1 cut(s) 52
Kzo9I GATC 3 cut(s) 30, 323, 618
LpnPI CCDG 9 cut(s) 53, 114, 236, 242, 243, 262, 368, 643, 670
LweI GCATC 2 cut(s) 259, 299
MaeI CTAG 2 cut(s) 114, 561
MaeIII GTNAC 4 cut(s) 283, 313, 424, 482
MalI GATC 3 cut(s) 32, 325, 620
MboI GATC 3 cut(s) 30, 323, 618
MboII GAAGA 3 cut(s) 117, 296, 577
MfeI CAATTG 1 cut(s) 349
MflI RGATCY 1 cut(s) 323
MluCI AATT 1 cut(s) 349
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 7 cut(s) 11, 36, 101, 414, 539, 593, 621
Mph1103I ATGCAT 1 cut(s) 139
MseI TTAA 2 cut(s) 153, 567
MslI CAYNNNNRTG 2 cut(s) 393, 422
MspR9I CCNGG 1 cut(s) 658
MunI CAATTG 1 cut(s) 349
Mva1269I GAATGC 1 cut(s) 474
MvaI CCWGG 1 cut(s) 658
MwoI GCNNNNNNNGC 3 cut(s) 58, 134, 253
NcoI CCATGG 1 cut(s) 175
NdeII GATC 3 cut(s) 30, 323, 618
NlaIII CATG 7 cut(s) 52, 141, 179, 379, 421, 500, 556
NlaIV GGNNCC 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 424
NsbI TGCGCA 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 3 cut(s) 52, 141, 500
NspV TTCGAA 1 cut(s) 184
PaeI GCATGC 1 cut(s) 141
PciI ACATGT 1 cut(s) 496
PctI GAATGC 1 cut(s) 474
PfeI GAWTC 3 cut(s) 186, 478, 653
PflFI GACNNNGTC 1 cut(s) 371
PflMI CCANNNNNTGG 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 656
PpuMI RGGWCCY 1 cut(s) 110
PscI ACATGT 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 656
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 1 cut(s) 656
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 4 cut(s) 110, 227, 385, 449
PspPPI RGGWCCY 1 cut(s) 110
PsrI GAACNNNNNNTAC 2 cut(s) 497, 529
PsuI RGATCY 1 cut(s) 323
PsyI GACNNNGTC 1 cut(s) 371
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 2 cut(s) 393, 422
SaqAI TTAA 2 cut(s) 153, 567
Sau3AI GATC 3 cut(s) 30, 323, 618
Sau96I GGNCC 4 cut(s) 110, 227, 385, 449
ScrFI CCNGG 1 cut(s) 658
SetI ASST 9 cut(s) 38, 89, 112, 218, 261, 281, 375, 454, 585
SfaNI GCATC 2 cut(s) 259, 299
SfcI CTRYAG 1 cut(s) 514
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 3 cut(s) 110, 227, 449
SmiMI CAYNNNNRTG 2 cut(s) 393, 422
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SphI GCATGC 1 cut(s) 141
Sse9I AATT 1 cut(s) 349
SspMI CTAG 2 cut(s) 114, 561
StyD4I CCNGG 1 cut(s) 656
StyI CCWWGG 2 cut(s) 175, 473
TaaI ACNGT 2 cut(s) 340, 430
TaqI TCGA 3 cut(s) 184, 309, 602
TasI AATT 1 cut(s) 349
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 3 cut(s) 186, 478, 653
Tru1I TTAA 2 cut(s) 153, 567
Tru9I TTAA 2 cut(s) 153, 567
TscAI CASTG 3 cut(s) 97, 200, 255
TseFI GTSAC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 569, 577, 694
TspGWI ACGGA 2 cut(s) 117, 501
TspRI CASTG 3 cut(s) 97, 200, 255
Tth111I GACNNNGTC 1 cut(s) 371
Van91I CCANNNNNTGG 1 cut(s) 351
VpaK11BI GGWCC 3 cut(s) 110, 227, 449
XagI CCTNNNNNAGG 1 cut(s) 40
XceI RCATGY 3 cut(s) 52, 141, 500
XspI CTAG 2 cut(s) 114, 561
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.