Prupe.1G565800_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008415
Physical Location & Seq
Reverse (-)
46179241 .. 46181639
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G565800.1

Sequence Viewer

Length: 1188 bp
ATGGCTTGTCATGGATGCCCCAATGGCAATGCCAATATCTGGTGCTTTGTTCTGGCCCTTTTGATCACCATTGCCAGCTTCGCTCCAAACCCAAGTTTTGCTAAGAAAACAAAAGTTGATGGCTTGAAATTGAACGTGATCGATGGTTGCTGGAGATGGAACTCGGATTGGAGAAGGAACCGGCAGGAACTTGCATTATGCTCAGTGGGATTTTCTGGGAAGATGAGCAACAACATAGGAAGAGATGTCATATACTATCAAGTCACGGAGCCAAGTGACAGTGCGTTGGACCCCAAACCGGGAACTTTGAGATATGCGGTTACCATGATCAAAGGGAAAAAGTGGATCACCTTCCGGAGGGACATGCACATTAGGCTTGACAAACCACTTCTCATTAGTAGCTTCACTGCCATTGATGGAAGAGGTGCTAGTGTTCACATTGCTGGTAATGCATGCTTGCTGGTCTTTCAGGCAAGCAATATAATCATCCATGGCCTTCGAATCCATCATTGCCGACCCCAACCACCAAGCTCAGTTATGGGTCCCGAAGGAAAGATAATGCCAATAGGGCAGGTTGATGGAGATGCAATCAGGTTGGTAACTGCATCAAAGGTTTGGATAGACCACAATACACTCTACGAGTGCCAGGACGGTCTTCTCGATGTTACTCGCGGATCTACCCATATCACCATCTCCAACAATTGGTTCAGAGACCAGGACAAGGTTATGCTTCTCGGCCATGATGATGGCTACTTTCGGGACAAGAACATGAGGGTGACTGTTGTGTACAACCATTTTGGACCCAACTGCAACCAAAGAATGCCAAGGATTCGCTATGGATATGCACATGTCGTGAACAACCTTTACAGAGAATGGTCGCAGTATGCTATTGGGGGAAGCATGAACCCTAGTGTTAAGAGTGAAGCCAACCTCTTCATTGCACCAAAATCGGGAAACAAGAAAGAGATCACTTGGAGGAAGGACAGCATTGGAGACAAAGAATCCTGGAAGTTTTACTCTGTAGGAGATATCTTTGAAAATGGGGCTTCTTTCGTGGAAACAGGTGCCGGCAGAGCAAAGCCAAACTATAACAGAGAACAAACTTTCCCAGTTGTTAATGCCAAATCTGTCCGCTCATTGACAAGGTCATCTGGTGCTTTGATATGCATCAAAAGATCCAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

396

Amino Acids

44.33

Weight (kDa)

9.55

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 1064
AccB7I CCANNNNNTGG 2 cut(s) 39, 702
AccBSI CCGCTC 1 cut(s) 1134
AccII CGCG 1 cut(s) 672
AccIII TCCGGA 1 cut(s) 354
AciI CCGC 3 cut(s) 317, 672, 1132
AclWI GGATC 3 cut(s) 353, 682, 1170
AcoI YGGCCR 1 cut(s) 736
AfaI GTAC 1 cut(s) 788
AfiI CCNNNNNNNGG 7 cut(s) 39, 298, 299, 357, 702, 721, 950
AflIII ACRYGT 1 cut(s) 847
AgsI TTSAA 3 cut(s) 127, 133, 1037
AjnI CCWGG 3 cut(s) 645, 714, 1004
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AloI GAACNNNNNNTCC 2 cut(s) 1089, 1121
AluBI AGCT 3 cut(s) 78, 402, 531
AluI AGCT 3 cut(s) 78, 402, 531
Alw26I GTCTC 2 cut(s) 705, 987
AlwI GGATC 3 cut(s) 353, 682, 1170
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 3 cut(s) 54, 493, 736
AspS9I GGNCC 4 cut(s) 55, 289, 542, 800
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 4 cut(s) 58, 340, 679, 787
AsuII TTCGAA 1 cut(s) 499
AvaII GGWCC 3 cut(s) 289, 542, 800
BanI GGYRCC 1 cut(s) 1064
BbsI GAAGAC 1 cut(s) 647
BccI CCATC 8 cut(s) 113, 137, 150, 410, 513, 572, 698, 740
BcgI CGANNNNNNTGC 2 cut(s) 930, 964
BciT130I CCWGG 3 cut(s) 647, 716, 1006
BclI TGATCA 2 cut(s) 63, 327
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 705, 987
BfaI CTAG 2 cut(s) 429, 909
BfmI CTRYAG 1 cut(s) 1020
BfuAI ACCTGC 1 cut(s) 562
BglI GCCNNNNNGGC 2 cut(s) 24, 568
Bme1390I CCNGG 4 cut(s) 300, 647, 716, 1006
Bme18I GGWCC 3 cut(s) 289, 542, 800
BmgT120I GGNCC 4 cut(s) 55, 289, 542, 800
BmiI GGNNCC 7 cut(s) 179, 270, 291, 543, 544, 802, 1066
BmrFI CCNGG 4 cut(s) 300, 647, 716, 1006
BmrI ACTGGG 1 cut(s) 1103
BmsI GCATC 5 cut(s) 5, 574, 614, 1172, 1176
BmuI ACTGGG 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 647
BpmI CTGGAG 1 cut(s) 172
Bpu14I TTCGAA 1 cut(s) 499
BpuMI CCSGG 1 cut(s) 300
Bsa29I ATCGAT 1 cut(s) 141
BsaBI GATNNNNATC 1 cut(s) 1166
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 490, 824
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 7 cut(s) 39, 298, 299, 357, 702, 721, 950
Bse118I RCCGGY 2 cut(s) 180, 1067
Bse1I ACTGG 1 cut(s) 1109
Bse3DI GCAATG 5 cut(s) 34, 69, 438, 508, 936
Bse8I GATNNNNATC 1 cut(s) 1166
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 3 cut(s) 647, 716, 1006
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 490, 824
BseGI GGATG 2 cut(s) 20, 486
BseJI GATNNNNATC 1 cut(s) 1166
BseLI CCNNNNNNNGG 7 cut(s) 39, 298, 299, 357, 702, 721, 950
BseMI GCAATG 5 cut(s) 34, 69, 438, 508, 936
BseMII CTCAG 2 cut(s) 216, 546
BseNI ACTGG 1 cut(s) 1109
Bsh1236I CGCG 1 cut(s) 672
BshFI GGCC 3 cut(s) 56, 495, 738
BshNI GGYRCC 1 cut(s) 1064
BshVI ATCGAT 1 cut(s) 141
BsiSI CCGG 4 cut(s) 181, 299, 355, 1068
BslFI GGGAC 3 cut(s) 374, 528, 773
BslI CCNNNNNNNGG 7 cut(s) 39, 298, 299, 357, 702, 721, 950
BsmAI GTCTC 2 cut(s) 705, 987
BsmFI GGGAC 3 cut(s) 374, 528, 773
BsmI GAATGC 1 cut(s) 825
BsnI GGCC 3 cut(s) 56, 495, 738
Bso31I GGTCTC 1 cut(s) 705
Bsp119I TTCGAA 1 cut(s) 499
Bsp13I TCCGGA 1 cut(s) 354
Bsp1407I TGTACA 1 cut(s) 786
Bsp143I GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 3 cut(s) 317, 672, 1132
BspANI GGCC 3 cut(s) 56, 495, 738
BspCNI CTCAG 2 cut(s) 215, 545
BspDI ATCGAT 1 cut(s) 141
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 1 cut(s) 672
BspLI GGNNCC 7 cut(s) 179, 270, 291, 543, 544, 802, 1066
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 3 cut(s) 353, 682, 1170
BspT104I TTCGAA 1 cut(s) 499
BspT107I GGYRCC 1 cut(s) 1064
BspTNI GGTCTC 1 cut(s) 705
BsrBI CCGCTC 1 cut(s) 1134
BsrDI GCAATG 5 cut(s) 34, 69, 438, 508, 936
BsrFI RCCGGY 2 cut(s) 180, 1067
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 1 cut(s) 1109
BssAI RCCGGY 2 cut(s) 180, 1067
BssECI CCNNGG 2 cut(s) 490, 824
BssMI GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
BssT1I CCWWGG 2 cut(s) 490, 824
Bst2UI CCWGG 3 cut(s) 647, 716, 1006
Bst4CI ACNGT 3 cut(s) 281, 653, 781
Bst6I CTCTTC 3 cut(s) 235, 415, 938
BstAUI TGTACA 1 cut(s) 786
BstBI TTCGAA 1 cut(s) 499
BstC8I GCNNGC 5 cut(s) 76, 454, 458, 475, 1069
BstDEI CTNAG 3 cut(s) 102, 202, 532
BstDSI CCRYGG 1 cut(s) 490
BstEII GGTNACC 1 cut(s) 319
BstENI CCTNNNNNAGG 1 cut(s) 355
BstF5I GGATG 2 cut(s) 20, 486
BstFNI CGCG 1 cut(s) 672
BstKTI GATC 7 cut(s) 66, 141, 330, 348, 677, 969, 1178
BstMAI GTCTC 2 cut(s) 705, 987
BstMBI GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
BstMWI GCNNNNNNNGC 6 cut(s) 24, 80, 373, 449, 568, 1073
BstNI CCWGG 3 cut(s) 647, 716, 1006
BstNSI RCATGY 3 cut(s) 367, 456, 851
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 4 cut(s) 298, 645, 714, 1004
BstSFI CTRYAG 1 cut(s) 1020
BstUI CGCG 1 cut(s) 672
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 2 cut(s) 674, 1175
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 2 cut(s) 674, 1175
Bsu15I ATCGAT 1 cut(s) 141
BsuRI GGCC 3 cut(s) 56, 495, 738
BsuTUI ATCGAT 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 2 cut(s) 20, 486
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 3 cut(s) 210, 286, 405
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 5 cut(s) 76, 454, 458, 475, 1069
Cfr10I RCCGGY 2 cut(s) 180, 1067
Cfr13I GGNCC 4 cut(s) 55, 289, 542, 800
ClaI ATCGAT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 787
CviAII CATG 9 cut(s) 11, 325, 364, 453, 491, 740, 769, 848, 901
CviQI GTAC 1 cut(s) 787
DdeI CTNAG 3 cut(s) 102, 202, 532
DpnI GATC 7 cut(s) 65, 140, 329, 347, 676, 968, 1177
DpnII GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
EaeI YGGCCR 1 cut(s) 736
Eam1104I CTCTTC 3 cut(s) 235, 415, 938
EarI CTCTTC 3 cut(s) 235, 415, 938
Eco130I CCWWGG 2 cut(s) 490, 824
Eco31I GGTCTC 1 cut(s) 705
Eco32I GATATC 1 cut(s) 1030
Eco47I GGWCC 3 cut(s) 289, 542, 800
Eco91I GGTNACC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 355
EcoO109I RGGNCCY 1 cut(s) 542
EcoO65I GGTNACC 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 645, 714, 1004
EcoRV GATATC 1 cut(s) 1030
EcoT14I CCWWGG 2 cut(s) 490, 824
EcoT22I ATGCAT 2 cut(s) 454, 1169
ErhI CCWWGG 2 cut(s) 490, 824
FaeI CATG 9 cut(s) 14, 328, 367, 456, 494, 743, 772, 851, 904
FaqI GGGAC 3 cut(s) 374, 528, 773
FatI CATG 9 cut(s) 10, 324, 363, 452, 490, 739, 768, 847, 900
FbaI TGATCA 2 cut(s) 63, 327
FokI GGATG 2 cut(s) 27, 473
FspBI CTAG 2 cut(s) 429, 909
GsuI CTGGAG 1 cut(s) 172
HaeIII GGCC 3 cut(s) 56, 495, 738
HapII CCGG 4 cut(s) 181, 299, 355, 1068
Hin1II CATG 9 cut(s) 14, 328, 367, 456, 494, 743, 772, 851, 904
HinfI GANTC 3 cut(s) 501, 829, 1001
HpaII CCGG 4 cut(s) 181, 299, 355, 1068
HphI GGTGA 4 cut(s) 58, 340, 679, 787
Hpy166II GTNNAC 3 cut(s) 436, 787, 856
Hpy188I TCNGA 2 cut(s) 166, 710
Hpy188III TCNNGA 7 cut(s) 355, 545, 659, 758, 853, 951, 1179
Hpy8I GTNNAC 3 cut(s) 436, 787, 856
HpyAV CCTTC 5 cut(s) 168, 361, 506, 542, 973
HpyCH4III ACNGT 3 cut(s) 281, 653, 781
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 9 cut(s) 194, 367, 452, 587, 605, 810, 845, 941, 1167
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 80, 373, 449, 568, 1073
HpyF3I CTNAG 3 cut(s) 102, 202, 532
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 9 cut(s) 14, 328, 367, 456, 494, 743, 772, 851, 904
KflI GGGWCCC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 354
KroI GCCGGC 1 cut(s) 1067
KroNI GCCGGC 1 cut(s) 1069
Ksp22I TGATCA 2 cut(s) 63, 327
Kzo9I GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
LmnI GCTCC 2 cut(s) 88, 268
LweI GCATC 5 cut(s) 5, 574, 614, 1172, 1176
MaeI CTAG 2 cut(s) 429, 909
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 6 cut(s) 262, 275, 319, 598, 664, 775
MalI GATC 7 cut(s) 65, 140, 329, 347, 676, 968, 1177
MbiI CCGCTC 1 cut(s) 1134
MboI GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
MboII GAAGA 5 cut(s) 232, 252, 432, 647, 925
MfeI CAATTG 1 cut(s) 700
MflI RGATCY 2 cut(s) 674, 1175
MluCI AATT 2 cut(s) 128, 700
MmeI TCCRAC 2 cut(s) 267, 720
MnlI CCTC 5 cut(s) 351, 416, 765, 941, 969
Mph1103I ATGCAT 2 cut(s) 454, 1169
MroI TCCGGA 1 cut(s) 354
MroNI GCCGGC 1 cut(s) 1067
MseI TTAA 2 cut(s) 915, 1116
MslI CAYNNNNRTG 2 cut(s) 744, 773
MspI CCGG 4 cut(s) 181, 299, 355, 1068
MspR9I CCNGG 4 cut(s) 300, 647, 716, 1006
MunI CAATTG 1 cut(s) 700
Mva1269I GAATGC 1 cut(s) 825
MvaI CCWGG 3 cut(s) 647, 716, 1006
MvnI CGCG 1 cut(s) 672
MwoI GCNNNNNNNGC 6 cut(s) 24, 80, 373, 449, 568, 1073
NaeI GCCGGC 1 cut(s) 1069
NciI CCSGG 1 cut(s) 300
NcoI CCATGG 1 cut(s) 490
NdeII GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
NgoMIV GCCGGC 1 cut(s) 1067
NlaIII CATG 9 cut(s) 14, 328, 367, 456, 494, 743, 772, 851, 904
NlaIV GGNNCC 7 cut(s) 179, 270, 291, 543, 544, 802, 1066
NmeAIII GCCGAG 1 cut(s) 714
NmuCI GTSAC 3 cut(s) 262, 275, 775
NsiI ATGCAT 2 cut(s) 454, 1169
NspI RCATGY 3 cut(s) 367, 456, 851
NspV TTCGAA 1 cut(s) 499
PaeI GCATGC 1 cut(s) 456
PciI ACATGT 1 cut(s) 847
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PctI GAATGC 1 cut(s) 825
PdiI GCCGGC 1 cut(s) 1069
PfeI GAWTC 3 cut(s) 501, 829, 1001
PflFI GACNNNGTC 1 cut(s) 1144
PflMI CCANNNNNTGG 2 cut(s) 39, 702
PfoI TCCNGGA 1 cut(s) 1004
PpuMI RGGWCCY 1 cut(s) 542
PscI ACATGT 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 645, 714, 1004
PspEI GGTNACC 1 cut(s) 319
PspGI CCWGG 3 cut(s) 645, 714, 1004
PspN4I GGNNCC 7 cut(s) 179, 270, 291, 543, 544, 802, 1066
PspPI GGNCC 4 cut(s) 55, 289, 542, 800
PspPPI RGGWCCY 1 cut(s) 542
PsrI GAACNNNNNNTAC 2 cut(s) 848, 880
PsuI RGATCY 2 cut(s) 674, 1175
PsyI GACNNNGTC 1 cut(s) 1144
RsaI GTAC 1 cut(s) 788
RsaNI GTAC 1 cut(s) 787
RseI CAYNNNNRTG 2 cut(s) 744, 773
SaqAI TTAA 2 cut(s) 915, 1116
Sau3AI GATC 7 cut(s) 63, 138, 327, 345, 674, 966, 1175
Sau96I GGNCC 4 cut(s) 55, 289, 542, 800
ScrFI CCNGG 4 cut(s) 300, 647, 716, 1006
SfaNI GCATC 5 cut(s) 5, 574, 614, 1172, 1176
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 1 cut(s) 499
SinI GGWCC 3 cut(s) 289, 542, 800
SmiMI CAYNNNNRTG 2 cut(s) 744, 773
SphI GCATGC 1 cut(s) 456
Sse9I AATT 2 cut(s) 128, 700
SsiI CCGC 3 cut(s) 317, 672, 1132
SspMI CTAG 2 cut(s) 429, 909
StyD4I CCNGG 4 cut(s) 298, 645, 714, 1004
StyI CCWWGG 2 cut(s) 490, 824
TaaI ACNGT 3 cut(s) 281, 653, 781
TaiI ACGT 1 cut(s) 138
TaqI TCGA 3 cut(s) 141, 499, 660
TasI AATT 2 cut(s) 128, 700
TatI WGTACW 1 cut(s) 786
TfiI GAWTC 3 cut(s) 501, 829, 1001
Tru1I TTAA 2 cut(s) 915, 1116
Tru9I TTAA 2 cut(s) 915, 1116
TscAI CASTG 3 cut(s) 210, 286, 412
TseFI GTSAC 3 cut(s) 262, 275, 775
Tsp45I GTSAC 3 cut(s) 262, 275, 775
TspDTI ATGAA 2 cut(s) 917, 925
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 3 cut(s) 210, 286, 412
Tth111I GACNNNGTC 1 cut(s) 1144
Van91I CCANNNNNTGG 2 cut(s) 39, 702
VpaK11BI GGWCC 3 cut(s) 289, 542, 800
XagI CCTNNNNNAGG 1 cut(s) 355
XceI RCATGY 3 cut(s) 367, 456, 851
XspI CTAG 2 cut(s) 429, 909
Zsp2I ATGCAT 2 cut(s) 454, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.