Rh7BG306500

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008415
Physical Location & Seq
Reverse (-)
29240306 .. 29241881
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG306500.1

Sequence Viewer

Length: 1248 bp
ATGGCTTCTCAATACTGCTTTGTTGTGAATATCATCATTCTCCTCTGCAGCTTGGGTCCGAACCTGGGTTTGGCAAATGAAACAAAAGTCCATGGCTTGAAAATGAACGTAATTGATGGTTGCTGGAGATGGAATCCAAATTGGAGAAGCAACCGCCAGCAACTTGCATTGTGTTCGGTAGGATTTGCCGGGAAAATGAGCAACAACATAGGAAAAGATGTCGTAAACTATGAAGTAACAGATCCCAGTGACAATGCATTAAATCCCCAACCAGGTACTTTGAGATATGGAGCTACTATGATCAAAAGCAAAAAATGGATCACATTCCAGAGGGACATGAAAATTAAACTCGAGAAGCCATTGCTCGTTAGTAGCTTCACTGCAATTGATGGAAGAGGTGCTAATGTCCACATACAAGGTAACGCGTGCTTGAGAGTGTTCAAAGCAACCAATGTTATCATACATGGCCTTCGAATCCACCACTGCAAGTCGCAGCCACCGACCACGGTTATGGGTCCGAATGGAATAGTTTCACTAGGAGCGGTTGATGGAGACGCAATAAGGTTGGTTACTGCATCAAAGGTTTGGATAGACCACAATACACTATACGAGTGTGAGGATGGTCTTCTCGACGTCACTCGTGGATCAACTGATGTCACCGTCTCCAACAACTGGTTCAGAAACCAGGACAAGGTAATGCTTCTCGGTCATGATGATGGCTTCTTTCGGGACAAGAACATGAAGGTGACTGTAGTGTACAATCATTTTGGACCACTCTGCAATCAAAGAATGCCAAGGATTCGCCATGGATATGCACATGTCGTAAACAACTTGTACAGGGAATGGACACAGTATGCAATTGGGGGAAGCATGAACCCTAGTGTTAAAAGCGAAGCCAACCTCTTCATTGCACCAAAACAAAACAAAGAGGCAAGTTTACAAAAATTAAATAATGCAGGCCACAAGAGGTATGTAAGCAATATATTGACGATGACAGAAACTTTCCTAACTCCTGGCAAACAAATTCCACAGATCACTTGGAGGCAGGACAGTGTTAACAACTCCTGGAAATTTTACTCTAAAGGCGATATTTTTGAAAATGGGGCTTCCTTCATCCAAACAGGTCAAGGTGGAGCCCAGCCACACTATAACATTGAACAAAATTTCCGAGTTGCTGACGCCAAATCTGTCCGGTCATTGACAAGGTCATCAGGTGCTCTAACATGCACCAAACAATCTAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

415

Amino Acids

46.38

Weight (kDa)

9.52

Isoelectric Point (pI)

38.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 122 - 318 1.6e-26 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 636
AccB7I CCANNNNNTGG 1 cut(s) 672
AccBSI CCGCTC 1 cut(s) 542
AccII CGCG 1 cut(s) 425
AciI CCGC 2 cut(s) 154, 542
AclWI GGATC 3 cut(s) 236, 326, 652
AcsI RAATTY 3 cut(s) 1023, 1070, 1162
AcyI GRCGYC 2 cut(s) 633, 1179
AfaI GTAC 3 cut(s) 277, 758, 836
AfiI CCNNNNNNNGG 5 cut(s) 65, 70, 272, 672, 691
AflIII ACRYGT 2 cut(s) 423, 817
AgsI TTSAA 4 cut(s) 100, 442, 1097, 1157
AjnI CCWGG 5 cut(s) 63, 271, 684, 1012, 1064
AjuI GAANNNNNNNTTGG 4 cut(s) 53, 85, 659, 691
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
AluBI AGCT 3 cut(s) 51, 293, 375
AluI AGCT 3 cut(s) 51, 293, 375
Alw21I GWGCWC 1 cut(s) 1219
Alw26I GTCTC 2 cut(s) 546, 667
AlwI GGATC 3 cut(s) 236, 326, 652
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 2 cut(s) 466, 958
ApeKI GCWGC 2 cut(s) 48, 493
ApoI RAATTY 3 cut(s) 1023, 1070, 1162
Asp700I GAANNNNTTC 1 cut(s) 529
AspS9I GGNCC 3 cut(s) 56, 515, 770
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 2 cut(s) 649, 757
AsuII TTCGAA 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 56, 515, 770
BanII GRGCYC 1 cut(s) 1138
BauI CACGAG 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 1219
BbvI GCAGC 2 cut(s) 60, 505
BccI CCATC 6 cut(s) 110, 123, 383, 542, 614, 710
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 5 cut(s) 65, 273, 686, 1014, 1066
BclI TGATCA 1 cut(s) 300
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 546, 667
BfaI CTAG 3 cut(s) 536, 879, 1239
BfmI CTRYAG 2 cut(s) 46, 750
BisI GCNGC 2 cut(s) 49, 494
BlsI GCNGC 2 cut(s) 50, 495
Bme1390I CCNGG 6 cut(s) 65, 190, 273, 686, 1014, 1066
Bme18I GGWCC 3 cut(s) 56, 515, 770
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 3 cut(s) 56, 515, 770
BmiI GGNNCC 3 cut(s) 57, 516, 1135
BmrFI CCNGG 6 cut(s) 65, 190, 273, 686, 1014, 1066
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 2 cut(s) 584, 1232
BmuI ACTGGG 1 cut(s) 240
BpiI GAAGAC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 145
Bpu14I TTCGAA 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 190
BsaHI GRCGYC 2 cut(s) 633, 1179
BsaJI CCNNGG 5 cut(s) 64, 91, 504, 794, 805
BsaWI WCCGGW 1 cut(s) 1191
Bsc4I CCNNNNNNNGG 5 cut(s) 65, 70, 272, 672, 691
Bse1I ACTGG 2 cut(s) 246, 677
Bse3DI GCAATG 2 cut(s) 359, 906
BseBI CCWGG 5 cut(s) 65, 273, 686, 1014, 1066
BseDI CCNNGG 5 cut(s) 64, 91, 504, 794, 805
BseGI GGATG 2 cut(s) 625, 1113
BseLI CCNNNNNNNGG 5 cut(s) 65, 70, 272, 672, 691
BseMI GCAATG 2 cut(s) 359, 906
BseNI ACTGG 2 cut(s) 246, 677
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 60, 505
BseYI CCCAGC 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 425
BshFI GGCC 2 cut(s) 468, 960
BsiHKAI GWGCWC 1 cut(s) 1219
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 2 cut(s) 189, 1192
BslFI GGGAC 2 cut(s) 347, 743
BslI CCNNNNNNNGG 5 cut(s) 65, 70, 272, 672, 691
BsmAI GTCTC 2 cut(s) 546, 667
BsmBI CGTCTC 2 cut(s) 546, 667
BsmFI GGGAC 2 cut(s) 347, 743
BsmI GAATGC 1 cut(s) 795
BsnI GGCC 2 cut(s) 468, 960
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 1138, 1219
Bsp1407I TGTACA 2 cut(s) 756, 834
Bsp143I GATC 5 cut(s) 241, 300, 318, 644, 1032
Bsp19I CCATGG 2 cut(s) 91, 805
BspACI CCGC 2 cut(s) 154, 542
BspANI GGCC 2 cut(s) 468, 960
BspFNI CGCG 1 cut(s) 425
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 3 cut(s) 57, 516, 1135
BspMAI CTGCAG 1 cut(s) 50
BspPI GGATC 3 cut(s) 236, 326, 652
BspT104I TTCGAA 1 cut(s) 472
BsrBI CCGCTC 1 cut(s) 542
BsrDI GCAATG 2 cut(s) 359, 906
BsrGI TGTACA 2 cut(s) 756, 834
BsrI ACTGG 2 cut(s) 246, 677
BssECI CCNNGG 5 cut(s) 64, 91, 504, 794, 805
BssMI GATC 5 cut(s) 241, 300, 318, 644, 1032
BssNI GRCGYC 2 cut(s) 633, 1179
BssSI CACGAG 1 cut(s) 639
BssT1I CCWWGG 3 cut(s) 91, 794, 805
Bst2BI CACGAG 1 cut(s) 639
Bst2UI CCWGG 5 cut(s) 65, 273, 686, 1014, 1066
Bst4CI ACNGT 5 cut(s) 508, 661, 751, 852, 1052
Bst6I CTCTTC 2 cut(s) 388, 908
BstACI GRCGYC 2 cut(s) 633, 1179
BstAUI TGTACA 2 cut(s) 756, 834
BstBI TTCGAA 1 cut(s) 472
BstC8I GCNNGC 3 cut(s) 158, 427, 958
BstDSI CCRYGG 3 cut(s) 91, 504, 805
BstF5I GGATG 2 cut(s) 625, 1113
BstFNI CGCG 1 cut(s) 425
BstKTI GATC 5 cut(s) 244, 303, 321, 647, 1035
BstMAI GTCTC 2 cut(s) 546, 667
BstMBI GATC 5 cut(s) 241, 300, 318, 644, 1032
BstNI CCWGG 5 cut(s) 65, 273, 686, 1014, 1066
BstNSI RCATGY 2 cut(s) 821, 1227
BstSCI CCNGG 6 cut(s) 63, 188, 271, 684, 1012, 1064
BstSFI CTRYAG 2 cut(s) 46, 750
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 60, 505
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 1 cut(s) 241
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 241
BsuRI GGCC 2 cut(s) 468, 960
BtgI CCRYGG 3 cut(s) 91, 504, 805
BtsCI GGATG 2 cut(s) 625, 1113
BtsI GCAGTG 2 cut(s) 378, 481
BtsIMutI CAGTG 4 cut(s) 253, 378, 481, 1057
Cac8I GCNNGC 3 cut(s) 158, 427, 958
CciI TCATGA 1 cut(s) 709
Cfr13I GGNCC 3 cut(s) 56, 515, 770
CseI GACGC 2 cut(s) 563, 1187
CsiI ACCWGGT 1 cut(s) 271
Csp6I GTAC 3 cut(s) 276, 757, 835
CviAII CATG 9 cut(s) 92, 337, 464, 710, 739, 806, 818, 871, 1224
CviQI GTAC 3 cut(s) 276, 757, 835
DpnI GATC 5 cut(s) 243, 302, 320, 646, 1034
DpnII GATC 5 cut(s) 241, 300, 318, 644, 1032
Eam1104I CTCTTC 2 cut(s) 388, 908
EarI CTCTTC 2 cut(s) 388, 908
Eco130I CCWWGG 3 cut(s) 91, 794, 805
Eco24I GRGCYC 1 cut(s) 1138
Eco47I GGWCC 3 cut(s) 56, 515, 770
Eco88I CYCGRG 1 cut(s) 350
EcoRII CCWGG 5 cut(s) 63, 271, 684, 1012, 1064
EcoT14I CCWWGG 3 cut(s) 91, 794, 805
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 1138
ErhI CCWWGG 3 cut(s) 91, 794, 805
Esp3I CGTCTC 2 cut(s) 546, 667
FaeI CATG 9 cut(s) 95, 340, 467, 713, 742, 809, 821, 874, 1227
FaqI GGGAC 2 cut(s) 347, 743
FatI CATG 9 cut(s) 91, 336, 463, 709, 738, 805, 817, 870, 1223
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 2 cut(s) 49, 494
FokI GGATG 2 cut(s) 632, 1100
FriOI GRGCYC 1 cut(s) 1138
Fsp4HI GCNGC 2 cut(s) 49, 494
FspBI CTAG 3 cut(s) 536, 879, 1239
GluI GCNGC 2 cut(s) 49, 494
GsaI CCCAGC 1 cut(s) 1141
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 2 cut(s) 468, 960
HapII CCGG 2 cut(s) 189, 1192
HgaI GACGC 2 cut(s) 563, 1187
Hin1I GRCGYC 2 cut(s) 633, 1179
Hin1II CATG 9 cut(s) 95, 340, 467, 713, 742, 809, 821, 874, 1227
HincII GTYRAC 1 cut(s) 1057
HindII GTYRAC 1 cut(s) 1057
HinfI GANTC 3 cut(s) 133, 474, 799
HpaI GTTAAC 1 cut(s) 1057
HpaII CCGG 2 cut(s) 189, 1192
HphI GGTGA 2 cut(s) 649, 757
Hpy166II GTNNAC 6 cut(s) 226, 409, 757, 826, 938, 1057
Hpy188I TCNGA 4 cut(s) 60, 519, 680, 1169
Hpy188III TCNNGA 6 cut(s) 328, 352, 629, 710, 728, 1239
Hpy8I GTNNAC 6 cut(s) 226, 409, 757, 826, 938, 1057
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 3 cut(s) 479, 736, 1120
HpyCH4III ACNGT 5 cut(s) 508, 661, 751, 852, 1052
HpyCH4IV ACGT 2 cut(s) 108, 633
HpySE526I ACGT 2 cut(s) 108, 633
Hsp92I GRCGYC 2 cut(s) 633, 1179
Hsp92II CATG 9 cut(s) 95, 340, 467, 713, 742, 809, 821, 874, 1227
Ksp22I TGATCA 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 1057
Kzo9I GATC 5 cut(s) 241, 300, 318, 644, 1032
LmnI GCTCC 3 cut(s) 290, 539, 1133
Lsp1109I GCAGC 2 cut(s) 60, 505
LweI GCATC 2 cut(s) 584, 1232
MabI ACCWGGT 1 cut(s) 271
MaeI CTAG 3 cut(s) 536, 879, 1239
MaeII ACGT 2 cut(s) 108, 633
MaeIII GTNAC 7 cut(s) 235, 248, 419, 568, 634, 655, 745
MalI GATC 5 cut(s) 243, 302, 320, 646, 1034
MbiI CCGCTC 1 cut(s) 542
MboI GATC 5 cut(s) 241, 300, 318, 644, 1032
MboII GAAGA 3 cut(s) 405, 617, 895
MfeI CAATTG 2 cut(s) 384, 858
MflI RGATCY 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 1138, 1219
MluCI AATT 9 cut(s) 111, 139, 342, 384, 858, 944, 1023, 1070, 1162
MluI ACGCGT 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 690
MnlI CCTC 8 cut(s) 53, 324, 389, 610, 911, 922, 960, 1035
Mph1103I ATGCAT 1 cut(s) 259
MroXI GAANNNNTTC 1 cut(s) 529
MseI TTAA 5 cut(s) 260, 345, 885, 947, 1056
MslI CAYNNNNRTG 4 cut(s) 509, 714, 743, 810
MspI CCGG 2 cut(s) 189, 1192
MspR9I CCNGG 6 cut(s) 65, 190, 273, 686, 1014, 1066
MunI CAATTG 2 cut(s) 384, 858
Mva1269I GAATGC 1 cut(s) 795
MvaI CCWGG 5 cut(s) 65, 273, 686, 1014, 1066
MvnI CGCG 1 cut(s) 425
NciI CCSGG 1 cut(s) 190
NcoI CCATGG 2 cut(s) 91, 805
NdeII GATC 5 cut(s) 241, 300, 318, 644, 1032
NlaIII CATG 9 cut(s) 95, 340, 467, 713, 742, 809, 821, 874, 1227
NlaIV GGNNCC 3 cut(s) 57, 516, 1135
NmuCI GTSAC 4 cut(s) 248, 634, 655, 745
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 2 cut(s) 821, 1227
NspV TTCGAA 1 cut(s) 472
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 709
PciI ACATGT 1 cut(s) 817
PcsI WCGNNNNNNNCGW 1 cut(s) 497
PctI GAATGC 1 cut(s) 795
PdmI GAANNNNTTC 1 cut(s) 529
PfeI GAWTC 3 cut(s) 133, 474, 799
PflFI GACNNNGTC 1 cut(s) 1204
PflMI CCANNNNNTGG 1 cut(s) 672
PfoI TCCNGGA 1 cut(s) 1064
PkrI GCNGC 2 cut(s) 50, 495
PscI ACATGT 1 cut(s) 817
Psp6I CCWGG 5 cut(s) 63, 271, 684, 1012, 1064
PspFI CCCAGC 1 cut(s) 1137
PspGI CCWGG 5 cut(s) 63, 271, 684, 1012, 1064
PspN4I GGNNCC 3 cut(s) 57, 516, 1135
PspPI GGNCC 3 cut(s) 56, 515, 770
PstI CTGCAG 1 cut(s) 50
PsuI RGATCY 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 1204
RsaI GTAC 3 cut(s) 277, 758, 836
RsaNI GTAC 3 cut(s) 276, 757, 835
RseI CAYNNNNRTG 4 cut(s) 509, 714, 743, 810
SaqAI TTAA 5 cut(s) 260, 345, 885, 947, 1056
SatI GCNGC 2 cut(s) 49, 494
Sau3AI GATC 5 cut(s) 241, 300, 318, 644, 1032
Sau96I GGNCC 3 cut(s) 56, 515, 770
ScrFI CCNGG 6 cut(s) 65, 190, 273, 686, 1014, 1066
SduI GDGCHC 2 cut(s) 1138, 1219
SexAI ACCWGGT 1 cut(s) 271
SfaNI GCATC 2 cut(s) 584, 1232
SfcI CTRYAG 2 cut(s) 46, 750
Sfr274I CTCGAG 1 cut(s) 350
SfuI TTCGAA 1 cut(s) 472
SinI GGWCC 3 cut(s) 56, 515, 770
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 4 cut(s) 509, 714, 743, 810
SmlI CTYRAG 2 cut(s) 350, 430
SmoI CTYRAG 2 cut(s) 350, 430
Sse9I AATT 9 cut(s) 111, 139, 342, 384, 858, 944, 1023, 1070, 1162
SsiI CCGC 2 cut(s) 154, 542
SspMI CTAG 3 cut(s) 536, 879, 1239
StyD4I CCNGG 6 cut(s) 63, 188, 271, 684, 1012, 1064
StyI CCWWGG 3 cut(s) 91, 794, 805
TaaI ACNGT 5 cut(s) 508, 661, 751, 852, 1052
TaiI ACGT 2 cut(s) 111, 636
TaqI TCGA 3 cut(s) 351, 472, 630
TaqII GACCGA 1 cut(s) 695
TasI AATT 9 cut(s) 111, 139, 342, 384, 858, 944, 1023, 1070, 1162
TatI WGTACW 2 cut(s) 756, 834
TfiI GAWTC 3 cut(s) 133, 474, 799
Tru1I TTAA 5 cut(s) 260, 345, 885, 947, 1056
Tru9I TTAA 5 cut(s) 260, 345, 885, 947, 1056
TscAI CASTG 4 cut(s) 253, 385, 488, 1057
TseFI GTSAC 4 cut(s) 248, 634, 655, 745
TseI GCWGC 2 cut(s) 48, 493
Tsp45I GTSAC 4 cut(s) 248, 634, 655, 745
TspDTI ATGAA 8 cut(s) 93, 119, 246, 353, 755, 887, 895, 1102
TspRI CASTG 4 cut(s) 253, 385, 488, 1057
Tth111I GACNNNGTC 1 cut(s) 1204
Van91I CCANNNNNTGG 1 cut(s) 672
VpaK11BI GGWCC 3 cut(s) 56, 515, 770
XapI RAATTY 3 cut(s) 1023, 1070, 1162
XbaI TCTAGA 1 cut(s) 1238
XceI RCATGY 2 cut(s) 821, 1227
XcmI CCANNNNNNNNNTGG 1 cut(s) 1035
XhoI CTCGAG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 529
XspI CTAG 3 cut(s) 536, 879, 1239
ZraI GACGTC 1 cut(s) 634
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.