Prupe.1G565000_v2.0.a1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0008415
Physical Location & Seq
Forward (+)
46127770 .. 46130765
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G565000.1

Sequence Viewer

Length: 1275 bp
ATGGTTGTGTTTGTGTTTTTTGGAAATTCTGGTTCCAAGTCCATGGAGCCCAGAGCTGCTCTCAGCAAAGCCGTCGACGAGGCTTCAAAATTGGCGGGAAAACAGCAGTACGAGAAACGTGCACAAAATGTAGTTGAAGAGAAAAACCTTGCTGTAGCTATTTCTGTGCATGAGAGAGTTGGAAAGTACATATATATAATGGATTGTCATGGATGCCCAAATGGCAATGCCAATATCTGGCGCTTTGTTCTGTGCCTTTTGATCGCCATTGCCGGCTTCGCTCCAAACCCGAGTTTAGCTAAGAAAACAAAAGTTGATGGGTTGAAATTGAATGTGATCGATGGTTGCTGGAGATGGAACTCGGATTGGAGAAGTAACCGGCAGGAACTTGCATTATGCTCAGTGGGATTTTCTGGGAAGATGAGCAACAACATAGGAAGAGATGTCATATACTATCAAGTCACGGACCCAAGTGACAATGCGTTGGACCCCAAACCGGGAACTTTGAGATATGGGGTTACCATGATCAAAGGGAAAAAGTGGATCACCTTCCAGAGGGACATGCGCATTAGGCTTGACAAACCACTTCTCATTAGTAGCTTCACTGCCATTGATGGAAGAGGTGCTAATGTTCACATTGCTGGTAATGCATGCTTGCTGGTCTTTCAGGTACAAGGAAAGATAATGCCAATAGGGCAGGTTGATGGAGATGCAATCAGGTTGGTAACTGCATCAAAGGTTTGGATAGACCACAATACACTCTACGAGTGCCAGGACGGTCTTCTCGATGTTACTCGCGGATCTACCCATATCACCATCTCCAACAATTGGTTCAGAGACCAGGACAAGGTTATGCTTCTCGGCCATGATGATGGCTACTTTCGGGACAAGAACATGAGGGTAACCGTTGTGTACAACCATTTTGGACCCAACTGCAACCAAAGAATGCCAAGGATTCGCTATGGATATGCACATGTCGTGAATAACCTTTACAGAGAATGGTCGCAGTATGCCATTGGGGGAAGCATGAACCCTAGTGTTAAGAGTGAAGCCAACCTCTTCATTGCACCAAAATCGGGAAACAAGAAAGAGATCACTTGGAGGAAGGACAGCGTTGGAGACAAAGAATCCTGGAACACTACTACAAAAAAGCAAAAAGACGACGGTAAATCACCGTCGTGTATTTGGTTTTTCAATCACTACAAGGAAAAACCCTTTTGGCGACCACCAGTTTTGGTCGCTAAAAACTGTCAATTCGTCGCCAAAACCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

425

Amino Acids

47.94

Weight (kDa)

9.48

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 688
AccB7I CCANNNNNTGG 2 cut(s) 237, 828
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 798
AciI CCGC 2 cut(s) 95, 798
AclWI GGATC 2 cut(s) 551, 808
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 1 cut(s) 25
AfaI GTAC 4 cut(s) 110, 188, 672, 914
AfiI CCNNNNNNNGG 7 cut(s) 237, 496, 497, 555, 828, 847, 1076
AflIII ACRYGT 1 cut(s) 973
AgsI TTSAA 5 cut(s) 87, 137, 325, 331, 1195
AjnI CCWGG 3 cut(s) 771, 840, 1130
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AleI CACNNNNGTG 1 cut(s) 1177
AluBI AGCT 4 cut(s) 56, 158, 299, 600
AluI AGCT 4 cut(s) 56, 158, 299, 600
Alw21I GWGCWC 1 cut(s) 124
Alw26I GTCTC 2 cut(s) 831, 1113
Alw44I GTGCAC 1 cut(s) 120
AlwI GGATC 2 cut(s) 551, 808
Ama87I CYCGRG 1 cut(s) 289
AoxI GGCC 1 cut(s) 862
ApaLI GTGCAC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 25
AspLEI GCGC 2 cut(s) 243, 567
AspS9I GGNCC 3 cut(s) 466, 487, 926
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 3 cut(s) 538, 805, 1164
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 3 cut(s) 466, 487, 926
BaeGI GKGCMC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 773
Bbv12I GWGCWC 1 cut(s) 124
BbvI GCAGC 1 cut(s) 43
BccI CCATC 7 cut(s) 311, 335, 348, 608, 698, 824, 866
BceAI ACGGC 1 cut(s) 56
BcgI CGANNNNNNTGC 6 cut(s) 55, 89, 101, 135, 1056, 1090
BciT130I CCWGG 3 cut(s) 773, 842, 1132
BclI TGATCA 1 cut(s) 525
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 2 cut(s) 831, 1113
BfaI CTAG 1 cut(s) 1035
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 688
BglI GCCNNNNNGGC 2 cut(s) 222, 694
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 4 cut(s) 498, 773, 842, 1132
Bme18I GGWCC 3 cut(s) 466, 487, 926
BmeT110I CYCGRG 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 466, 487, 926
BmiI GGNNCC 5 cut(s) 34, 48, 468, 489, 928
BmrFI CCNGG 4 cut(s) 498, 773, 842, 1132
BmsI GCATC 3 cut(s) 203, 700, 740
BpiI GAAGAC 1 cut(s) 773
BplI GAGNNNNNCTC 2 cut(s) 45, 77
BpmI CTGGAG 1 cut(s) 370
BpuMI CCSGG 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 42, 950
Bsc4I CCNNNNNNNGG 7 cut(s) 237, 496, 497, 555, 828, 847, 1076
Bse118I RCCGGY 2 cut(s) 272, 378
Bse1I ACTGG 1 cut(s) 1229
Bse3DI GCAATG 4 cut(s) 232, 267, 636, 1062
BseBI CCWGG 3 cut(s) 773, 842, 1132
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 42, 950
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 7 cut(s) 237, 496, 497, 555, 828, 847, 1076
BseMI GCAATG 4 cut(s) 232, 267, 636, 1062
BseMII CTCAG 2 cut(s) 76, 414
BseNI ACTGG 1 cut(s) 1229
BseSI GKGCMC 1 cut(s) 124
BseXI GCAGC 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 798
BshFI GGCC 1 cut(s) 864
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 3 cut(s) 273, 379, 497
BslFI GGGAC 2 cut(s) 572, 899
BslI CCNNNNNNNGG 7 cut(s) 237, 496, 497, 555, 828, 847, 1076
BsmAI GTCTC 2 cut(s) 831, 1113
BsmFI GGGAC 2 cut(s) 572, 899
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 1 cut(s) 864
Bso31I GGTCTC 1 cut(s) 831
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 2 cut(s) 51, 124
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 95, 798
BspANI GGCC 1 cut(s) 864
BspCNI CTCAG 2 cut(s) 75, 413
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 1 cut(s) 798
BspLI GGNNCC 5 cut(s) 34, 48, 468, 489, 928
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 2 cut(s) 551, 808
BspTNI GGTCTC 1 cut(s) 831
BsrDI GCAATG 4 cut(s) 232, 267, 636, 1062
BsrFI RCCGGY 2 cut(s) 272, 378
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 1 cut(s) 1229
BssAI RCCGGY 2 cut(s) 272, 378
BssECI CCNNGG 2 cut(s) 42, 950
BssMI GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
BssT1I CCWWGG 2 cut(s) 42, 950
Bst2UI CCWGG 3 cut(s) 773, 842, 1132
Bst4CI ACNGT 5 cut(s) 779, 907, 1166, 1176, 1250
Bst6I CTCTTC 4 cut(s) 132, 433, 613, 1064
BstAUI TGTACA 1 cut(s) 912
BstC8I GCNNGC 3 cut(s) 274, 652, 656
BstDEI CTNAG 3 cut(s) 62, 300, 400
BstDSI CCRYGG 1 cut(s) 42
BstEII GGTNACC 2 cut(s) 517, 901
BstENI CCTNNNNNAGG 1 cut(s) 553
BstF5I GGATG 1 cut(s) 218
BstFNI CGCG 1 cut(s) 798
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 2 cut(s) 243, 567
BstKTI GATC 6 cut(s) 264, 339, 528, 546, 803, 1095
BstMAI GTCTC 2 cut(s) 831, 1113
BstMBI GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
BstMWI GCNNNNNNNGC 5 cut(s) 222, 278, 571, 647, 694
BstNI CCWGG 3 cut(s) 773, 842, 1132
BstNSI RCATGY 3 cut(s) 565, 654, 977
BstPI GGTNACC 2 cut(s) 517, 901
BstSCI CCNGG 4 cut(s) 496, 771, 840, 1130
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 124
BstUI CGCG 1 cut(s) 798
BstV1I GCAGC 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 773
BstX2I RGATCY 1 cut(s) 800
BstXI CCANNNNNNTGG 2 cut(s) 43, 872
BstYI RGATCY 1 cut(s) 800
Bsu15I ATCGAT 1 cut(s) 339
BsuRI GGCC 1 cut(s) 864
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 1 cut(s) 218
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 408, 603
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 3 cut(s) 274, 652, 656
CfoI GCGC 2 cut(s) 243, 567
Cfr10I RCCGGY 2 cut(s) 272, 378
Cfr13I GGNCC 3 cut(s) 466, 487, 926
ClaI ATCGAT 1 cut(s) 339
Csp6I GTAC 4 cut(s) 109, 187, 671, 913
CspCI CAANNNNNGTGG 2 cut(s) 1215, 1250
CviQI GTAC 4 cut(s) 109, 187, 671, 913
DdeI CTNAG 3 cut(s) 62, 300, 400
DpnI GATC 6 cut(s) 263, 338, 527, 545, 802, 1094
DpnII GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 4 cut(s) 132, 433, 613, 1064
EarI CTCTTC 4 cut(s) 132, 433, 613, 1064
Eco130I CCWWGG 2 cut(s) 42, 950
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 831
Eco47I GGWCC 3 cut(s) 466, 487, 926
Eco88I CYCGRG 1 cut(s) 289
Eco91I GGTNACC 2 cut(s) 517, 901
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoO65I GGTNACC 2 cut(s) 517, 901
EcoRII CCWGG 3 cut(s) 771, 840, 1130
EcoT14I CCWWGG 2 cut(s) 42, 950
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 2 cut(s) 42, 950
FaqI GGGAC 2 cut(s) 572, 899
FauI CCCGC 1 cut(s) 88
FbaI TGATCA 1 cut(s) 525
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 1 cut(s) 225
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 1 cut(s) 57
FspAI RTGCGCAY 1 cut(s) 566
FspBI CTAG 1 cut(s) 1035
FspI TGCGCA 1 cut(s) 566
GlaI GCGC 2 cut(s) 242, 566
GluI GCNGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 1 cut(s) 244
HaeIII GGCC 1 cut(s) 864
HapII CCGG 3 cut(s) 273, 379, 497
HhaI GCGC 2 cut(s) 243, 567
Hin6I GCGC 2 cut(s) 241, 565
HinP1I GCGC 2 cut(s) 241, 565
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HinfI GANTC 2 cut(s) 955, 1127
HpaII CCGG 3 cut(s) 273, 379, 497
HphI GGTGA 3 cut(s) 538, 805, 1164
Hpy166II GTNNAC 4 cut(s) 76, 122, 634, 913
Hpy188I TCNGA 2 cut(s) 364, 836
Hpy188III TCNNGA 5 cut(s) 553, 785, 884, 979, 1077
Hpy8I GTNNAC 4 cut(s) 76, 122, 634, 913
Hpy99I CGWCG 5 cut(s) 77, 80, 1166, 1180, 1262
HpyAV CCTTC 2 cut(s) 559, 1099
HpyCH4III ACNGT 5 cut(s) 779, 907, 1166, 1176, 1250
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 9 cut(s) 122, 169, 392, 650, 713, 731, 936, 971, 1067
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 278, 571, 647, 694
HpyF3I CTNAG 3 cut(s) 62, 300, 400
HpySE526I ACGT 1 cut(s) 118
HspAI GCGC 2 cut(s) 241, 565
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 525
Kzo9I GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
LmnI GCTCC 2 cut(s) 46, 286
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 203, 700, 740
MaeI CTAG 1 cut(s) 1035
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 7 cut(s) 374, 460, 473, 517, 724, 790, 901
MalI GATC 6 cut(s) 263, 338, 527, 545, 802, 1094
MboI GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
MboII GAAGA 6 cut(s) 149, 430, 450, 630, 773, 1051
MfeI CAATTG 1 cut(s) 826
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 2 cut(s) 51, 124
MluCI AATT 5 cut(s) 25, 89, 326, 826, 1253
MmeI TCCRAC 4 cut(s) 160, 465, 846, 1096
MnlI CCTC 6 cut(s) 73, 549, 614, 891, 1067, 1095
Mph1103I ATGCAT 1 cut(s) 652
MroNI GCCGGC 1 cut(s) 272
MseI TTAA 1 cut(s) 1041
MslI CAYNNNNRTG 2 cut(s) 870, 1177
MspI CCGG 3 cut(s) 273, 379, 497
MspR9I CCNGG 4 cut(s) 498, 773, 842, 1132
MunI CAATTG 1 cut(s) 826
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 3 cut(s) 773, 842, 1132
MvnI CGCG 1 cut(s) 798
MwoI GCNNNNNNNGC 5 cut(s) 222, 278, 571, 647, 694
NaeI GCCGGC 1 cut(s) 274
NciI CCSGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 42
NdeII GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
NgoMIV GCCGGC 1 cut(s) 272
NlaIV GGNNCC 5 cut(s) 34, 48, 468, 489, 928
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 2 cut(s) 460, 473
NsbI TGCGCA 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 652
NspI RCATGY 3 cut(s) 565, 654, 977
OliI CACNNNNGTG 1 cut(s) 1177
PaeI GCATGC 1 cut(s) 654
PciI ACATGT 1 cut(s) 973
PcsI WCGNNNNNNNCGW 1 cut(s) 783
PctI GAATGC 1 cut(s) 951
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 955, 1127
PflMI CCANNNNNTGG 2 cut(s) 237, 828
PfoI TCCNGGA 1 cut(s) 1130
PkrI GCNGC 1 cut(s) 58
PscI ACATGT 1 cut(s) 973
Psp6I CCWGG 3 cut(s) 771, 840, 1130
PspEI GGTNACC 2 cut(s) 517, 901
PspGI CCWGG 3 cut(s) 771, 840, 1130
PspN4I GGNNCC 5 cut(s) 34, 48, 468, 489, 928
PspPI GGNCC 3 cut(s) 466, 487, 926
PsuI RGATCY 1 cut(s) 800
RsaI GTAC 4 cut(s) 110, 188, 672, 914
RsaNI GTAC 4 cut(s) 109, 187, 671, 913
RseI CAYNNNNRTG 2 cut(s) 870, 1177
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 1041
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 6 cut(s) 261, 336, 525, 543, 800, 1092
Sau96I GGNCC 3 cut(s) 466, 487, 926
ScrFI CCNGG 4 cut(s) 498, 773, 842, 1132
SduI GDGCHC 2 cut(s) 51, 124
SfaNI GCATC 3 cut(s) 203, 700, 740
SfcI CTRYAG 1 cut(s) 153
SgrDI CGTCGACG 1 cut(s) 74
SinI GGWCC 3 cut(s) 466, 487, 926
SmiMI CAYNNNNRTG 2 cut(s) 870, 1177
SphI GCATGC 1 cut(s) 654
Sse9I AATT 5 cut(s) 25, 89, 326, 826, 1253
SsiI CCGC 2 cut(s) 95, 798
SspMI CTAG 1 cut(s) 1035
StyD4I CCNGG 4 cut(s) 496, 771, 840, 1130
StyI CCWWGG 2 cut(s) 42, 950
TaaI ACNGT 5 cut(s) 779, 907, 1166, 1176, 1250
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 75, 339, 786
TasI AATT 5 cut(s) 25, 89, 326, 826, 1253
TatI WGTACW 2 cut(s) 186, 912
TfiI GAWTC 2 cut(s) 955, 1127
Tru1I TTAA 1 cut(s) 1041
Tru9I TTAA 1 cut(s) 1041
TscAI CASTG 2 cut(s) 408, 610
TseFI GTSAC 2 cut(s) 460, 473
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 2 cut(s) 460, 473
TspDTI ATGAA 2 cut(s) 1043, 1051
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 2 cut(s) 408, 610
Van91I CCANNNNNTGG 2 cut(s) 237, 828
VneI GTGCAC 1 cut(s) 120
VpaK11BI GGWCC 3 cut(s) 466, 487, 926
XagI CCTNNNNNAGG 1 cut(s) 553
XapI RAATTY 1 cut(s) 25
XceI RCATGY 3 cut(s) 565, 654, 977
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 1035
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.