RLG00000008303

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008415
Physical Location & Seq
Reverse (-)
28647652 .. 28648526
875 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008303

Sequence Viewer

Length: 552 bp
ATGTGCTTAGTAGGATTTGCTGGGAAAATGAGCAATAATATAGAAAAAGACGTTATAAACTATGAAGTGATCGACGCTAGTGACAATGCTTTAGACCCAAAACCAGGAACCTTGAGATATGAAGCTACCATGTTGAAAGGAAAAAAATGGATCACATTCCAAAGGGACTTAAAAATTAAGCTCGAGAAACCACTACTCATTAGTAGCTTTACTACCATTGACGGAAAAGGTGCTAACATCCATATTGCTGGTAACGCTTGCTTGAGGGTGTTTAGGAAAACCAATGTGATTATACATGGCCATCGGATCCACCATTGCAAGTCACAACCATCGAGTTTAGTTATGGGTCCAAATGGATTAATATTACTAGGTGGGGTTGATGGAGATGCAATAAGGTTGGTTATCACATCAAAGATTTGGATAGATTGTAATACATTGTATGAGTGTGAGAATGGTCTTCTTGATGTCACTTGTGGATCAACCAATGTCACCATCTCCAACAATTGGTTTAGAGACCAGGACAAGGTAATTAATGCTCCTAGGTCACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

184

Amino Acids

20.28

Weight (kDa)

8.92

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 504
AclWI GGATC 4 cut(s) 158, 301, 314, 484
AcoI YGGCCR 1 cut(s) 298
AfiI CCNNNNNNNGG 3 cut(s) 104, 504, 523
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 2 cut(s) 103, 516
AluBI AGCT 3 cut(s) 125, 181, 207
AluI AGCT 3 cut(s) 125, 181, 207
Alw26I GTCTC 1 cut(s) 507
AlwI GGATC 4 cut(s) 158, 301, 314, 484
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 1 cut(s) 298
AseI ATTAAT 2 cut(s) 359, 531
AspA2I CCTAGG 1 cut(s) 539
AspS9I GGNCC 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 481
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 347
AvrII CCTAGG 1 cut(s) 539
BalI TGGCCA 1 cut(s) 300
BamHI GGATCC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 449
BccI CCATC 4 cut(s) 309, 337, 374, 500
BciT130I CCWGG 2 cut(s) 105, 518
BcoDI GTCTC 1 cut(s) 507
BfaI CTAG 3 cut(s) 78, 368, 540
BlnI CCTAGG 1 cut(s) 539
Bme1390I CCNGG 2 cut(s) 105, 518
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 3 cut(s) 109, 308, 348
BmrFI CCNGG 2 cut(s) 105, 518
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 449
BpuEI CTTGAG 2 cut(s) 133, 283
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 539
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 504, 523
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 105, 518
BseDI CCNNGG 1 cut(s) 539
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 3 cut(s) 104, 504, 523
BseMI GCAATG 1 cut(s) 313
BseYI CCCAGC 1 cut(s) 20
BshFI GGCC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 3 cut(s) 104, 504, 523
BsmAI GTCTC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 1 cut(s) 300
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 182
Bsp143I GATC 4 cut(s) 69, 150, 306, 476
BspANI GGCC 1 cut(s) 300
BspLI GGNNCC 3 cut(s) 109, 308, 348
BspPI GGATC 4 cut(s) 158, 301, 314, 484
BspTNI GGTCTC 1 cut(s) 507
BsrDI GCAATG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 539
BssMI GATC 4 cut(s) 69, 150, 306, 476
BssT1I CCWWGG 1 cut(s) 539
Bst2UI CCWGG 2 cut(s) 105, 518
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 1 cut(s) 7
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 4 cut(s) 72, 153, 309, 479
BstMAI GTCTC 1 cut(s) 507
BstMBI GATC 4 cut(s) 69, 150, 306, 476
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 105, 518
BstSCI CCNGG 2 cut(s) 103, 516
BstV2I GAAGAC 1 cut(s) 449
BstX2I RGATCY 1 cut(s) 306
BstXI CCANNNNNNTGG 1 cut(s) 248
BstYI RGATCY 1 cut(s) 306
BsuRI GGCC 1 cut(s) 300
BtsCI GGATG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 347
CseI GACGC 1 cut(s) 83
CviAII CATG 2 cut(s) 130, 296
CviJI RGCY 4 cut(s) 125, 181, 207, 300
CviKI_1 RGCY 4 cut(s) 125, 181, 207, 300
DdeI CTNAG 1 cut(s) 7
DpnI GATC 4 cut(s) 71, 152, 308, 478
DpnII GATC 4 cut(s) 69, 150, 306, 476
EaeI YGGCCR 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 539
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 103, 516
EcoT14I CCWWGG 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 539
FaeI CATG 2 cut(s) 133, 299
FaqI GGGAC 1 cut(s) 179
FatI CATG 2 cut(s) 129, 295
FokI GGATG 1 cut(s) 224
FspBI CTAG 3 cut(s) 78, 368, 540
GsaI CCCAGC 1 cut(s) 24
HaeIII GGCC 1 cut(s) 300
HgaI GACGC 1 cut(s) 83
Hin1II CATG 2 cut(s) 133, 299
HphI GGTGA 1 cut(s) 481
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 3 cut(s) 184, 461, 546
Hpy99I CGWCG 1 cut(s) 77
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 2 cut(s) 318, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 1 cut(s) 7
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 2 cut(s) 133, 299
Kzo9I GATC 4 cut(s) 69, 150, 306, 476
LmnI GCTCC 1 cut(s) 541
LpnPI CCDG 6 cut(s) 6, 90, 117, 234, 503, 530
LweI GCATC 1 cut(s) 376
MaeI CTAG 3 cut(s) 78, 368, 540
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 6 cut(s) 80, 251, 321, 466, 487, 543
MalI GATC 4 cut(s) 71, 152, 308, 478
MboI GATC 4 cut(s) 69, 150, 306, 476
MboII GAAGA 1 cut(s) 449
MfeI CAATTG 1 cut(s) 502
MflI RGATCY 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 3 cut(s) 174, 502, 528
MluNI TGGCCA 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 1 cut(s) 258
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 4 cut(s) 170, 177, 359, 531
Msp20I TGGCCA 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 105, 518
MunI CAATTG 1 cut(s) 502
MvaI CCWGG 2 cut(s) 105, 518
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 4 cut(s) 69, 150, 306, 476
NlaIII CATG 2 cut(s) 133, 299
NlaIV GGNNCC 3 cut(s) 109, 308, 348
NmuCI GTSAC 5 cut(s) 80, 321, 466, 487, 543
PaeR7I CTCGAG 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 504
PshBI ATTAAT 2 cut(s) 359, 531
PsiI TTATAA 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 103, 516
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 2 cut(s) 103, 516
PspN4I GGNNCC 3 cut(s) 109, 308, 348
PspPI GGNCC 1 cut(s) 347
PsuI RGATCY 1 cut(s) 306
SaqAI TTAA 4 cut(s) 170, 177, 359, 531
Sau3AI GATC 4 cut(s) 69, 150, 306, 476
Sau96I GGNCC 1 cut(s) 347
ScrFI CCNGG 2 cut(s) 105, 518
SfaNI GCATC 1 cut(s) 376
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 3 cut(s) 112, 182, 262
SmoI CTYRAG 3 cut(s) 112, 182, 262
Sse9I AATT 3 cut(s) 174, 502, 528
SspI AATATT 1 cut(s) 363
SspMI CTAG 3 cut(s) 78, 368, 540
StyD4I CCNGG 2 cut(s) 103, 516
StyI CCWWGG 1 cut(s) 539
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 72, 183, 332
TasI AATT 3 cut(s) 174, 502, 528
Tru1I TTAA 4 cut(s) 170, 177, 359, 531
Tru9I TTAA 4 cut(s) 170, 177, 359, 531
TseFI GTSAC 5 cut(s) 80, 321, 466, 487, 543
Tsp45I GTSAC 5 cut(s) 80, 321, 466, 487, 543
TspDTI ATGAA 2 cut(s) 78, 135
TspGWI ACGGA 1 cut(s) 237
Van91I CCANNNNNTGG 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 347
VspI ATTAAT 2 cut(s) 359, 531
XhoI CTCGAG 1 cut(s) 182
XmaJI CCTAGG 1 cut(s) 539
XspI CTAG 3 cut(s) 78, 368, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.