pycom06g00080

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008415
Physical Location & Seq
Forward (+)
66025 .. 66705
681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00080.1

Sequence Viewer

Length: 504 bp
ATGGGTCCTGATGGAAAGCCATTTCCAGTAGGCCAAGTTGATGGAGATGCAGTCAGGTTAATTACTGCATCCAAGGTTTGGATAGACCACAATACTTTGTATGAGTGCGAGGACGGTCTTCTTGACGTTACTTGTGGATCAACCGATATCACCATCTCCAACAATTGGCTCAGAGAGCAGGACAAGGTTATGCTCCTTGGCCACGATGATGGGTACGTTCGGGACAAGAACAAGAGAGTCACACTAGTGTACAATCATTTTGGACCCAACTGCAACCAAAGAATGCCAAGGATTCGATACGGATATGCACATGTGGTGAACAATTTGTACAGAAAATGGTCACAGCATGCAATCGGAGGAAGCATGAATCCTAGCGTTAAGAGTGAAGCCAACCTCTTTATTGCCCCAAAACGAGGAAACAAGGAGGTCACTTGGAGGAAGGTCAGCAACAGCATTGGAAGCAACAGCTCGTGGAAATTTCATTCTGTAGGAGATATTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

168

Amino Acids

18.87

Weight (kDa)

8.99

Isoelectric Point (pI)

22.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 14 - 157 2.9e-24 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 78, 165
AclWI GGATC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 3 cut(s) 215, 251, 329
AfiI CCNNNNNNNGG 3 cut(s) 78, 165, 413
AflIII ACRYGT 1 cut(s) 310
AhlI ACTAGT 1 cut(s) 244
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 1 cut(s) 468
AluI AGCT 1 cut(s) 468
AlwI GGATC 1 cut(s) 145
AoxI GGCC 2 cut(s) 31, 199
ApoI RAATTY 1 cut(s) 476
AspS9I GGNCC 2 cut(s) 5, 263
AsuHPI GGTGA 2 cut(s) 142, 328
AvaII GGWCC 2 cut(s) 5, 263
BalI TGGCCA 1 cut(s) 201
BauI CACGAG 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 110
BccI CCATC 4 cut(s) 5, 35, 161, 203
BcuI ACTAGT 1 cut(s) 244
BfaI CTAG 2 cut(s) 245, 372
BfmI CTRYAG 1 cut(s) 486
Bme18I GGWCC 2 cut(s) 5, 263
BmgT120I GGNCC 2 cut(s) 5, 263
BmiI GGNNCC 2 cut(s) 6, 265
BmsI GCATC 2 cut(s) 37, 77
BpiI GAAGAC 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 72, 196, 287
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 165, 413
Bse1I ACTGG 1 cut(s) 26
BseDI CCNNGG 3 cut(s) 72, 196, 287
BseGI GGATG 1 cut(s) 68
BseLI CCNNNNNNNGG 3 cut(s) 78, 165, 413
BseMII CTCAG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 26
BshFI GGCC 2 cut(s) 33, 201
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 3 cut(s) 78, 165, 413
BsmFI GGGAC 1 cut(s) 236
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 2 cut(s) 33, 201
Bsp1407I TGTACA 2 cut(s) 249, 327
Bsp143I GATC 1 cut(s) 137
BspANI GGCC 2 cut(s) 33, 201
BspCNI CTCAG 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 6, 265
BspPI GGATC 1 cut(s) 145
BsrGI TGTACA 2 cut(s) 249, 327
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 72, 196, 287
BssMI GATC 1 cut(s) 137
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 3 cut(s) 72, 196, 287
Bst2BI CACGAG 1 cut(s) 469
Bst4CI ACNGT 1 cut(s) 116
BstAUI TGTACA 2 cut(s) 249, 327
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 1 cut(s) 140
BstMBI GATC 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 175, 459
BstNSI RCATGY 2 cut(s) 314, 350
BstSFI CTRYAG 1 cut(s) 486
BstV2I GAAGAC 1 cut(s) 110
BstXI CCANNNNNNTGG 2 cut(s) 41, 209
BsuRI GGCC 2 cut(s) 33, 201
BtsCI GGATG 1 cut(s) 68
Cac8I GCNNGC 1 cut(s) 348
Cfr13I GGNCC 2 cut(s) 5, 263
Csp6I GTAC 3 cut(s) 214, 250, 328
CviAII CATG 3 cut(s) 311, 347, 364
CviJI RGCY 6 cut(s) 19, 33, 169, 201, 389, 468
CviKI_1 RGCY 6 cut(s) 19, 33, 169, 201, 389, 468
CviQI GTAC 3 cut(s) 214, 250, 328
DdeI CTNAG 1 cut(s) 170
DpnI GATC 1 cut(s) 139
DpnII GATC 1 cut(s) 137
EaeI YGGCCR 1 cut(s) 199
Eco130I CCWWGG 3 cut(s) 72, 196, 287
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 2 cut(s) 5, 263
EcoO109I RGGNCCY 1 cut(s) 5
EcoRV GATATC 1 cut(s) 148
EcoT14I CCWWGG 3 cut(s) 72, 196, 287
ErhI CCWWGG 3 cut(s) 72, 196, 287
FaeI CATG 3 cut(s) 314, 350, 367
FaiI YATR 6 cut(s) 102, 191, 306, 312, 348, 365
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 310, 346, 363
FokI GGATG 1 cut(s) 55
FspBI CTAG 2 cut(s) 245, 372
HaeIII GGCC 2 cut(s) 33, 201
Hin1II CATG 3 cut(s) 314, 350, 367
HinfI GANTC 3 cut(s) 237, 292, 367
HphI GGTGA 2 cut(s) 142, 328
Hpy166II GTNNAC 2 cut(s) 250, 319
Hpy188I TCNGA 2 cut(s) 173, 356
Hpy188III TCNNGA 3 cut(s) 8, 122, 221
Hpy8I GTNNAC 2 cut(s) 250, 319
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 2 cut(s) 126, 216
HpyCH4V TGCA 5 cut(s) 50, 68, 273, 308, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 175, 459
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 2 cut(s) 126, 216
Hsp92II CATG 3 cut(s) 314, 350, 367
Kzo9I GATC 1 cut(s) 137
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 4 cut(s) 21, 39, 40, 164
LweI GCATC 2 cut(s) 37, 77
MaeI CTAG 2 cut(s) 245, 372
MaeII ACGT 2 cut(s) 126, 216
MaeIII GTNAC 4 cut(s) 127, 238, 339, 427
MalI GATC 1 cut(s) 139
MboI GATC 1 cut(s) 137
MboII GAAGA 1 cut(s) 110
MfeI CAATTG 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 4 cut(s) 60, 163, 322, 476
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 6 cut(s) 103, 350, 404, 407, 418, 429
Mox20I TGGCCA 1 cut(s) 201
MscI TGGCCA 1 cut(s) 201
MseI TTAA 3 cut(s) 59, 378, 502
MslI CAYNNNNRTG 2 cut(s) 207, 245
Msp20I TGGCCA 1 cut(s) 201
MunI CAATTG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 175, 459
NdeII GATC 1 cut(s) 137
NlaIII CATG 3 cut(s) 314, 350, 367
NlaIV GGNNCC 2 cut(s) 6, 265
NmuCI GTSAC 3 cut(s) 238, 339, 427
NspI RCATGY 2 cut(s) 314, 350
OliI CACNNNNGTG 1 cut(s) 245
PaeI GCATGC 1 cut(s) 350
PciI ACATGT 1 cut(s) 310
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 292, 367
PflMI CCANNNNNTGG 2 cut(s) 78, 165
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 5
PspN4I GGNNCC 2 cut(s) 6, 265
PspPI GGNCC 2 cut(s) 5, 263
PspPPI RGGWCCY 1 cut(s) 5
PsrI GAACNNNNNNTAC 2 cut(s) 311, 343
RsaI GTAC 3 cut(s) 215, 251, 329
RsaNI GTAC 3 cut(s) 214, 250, 328
RseI CAYNNNNRTG 2 cut(s) 207, 245
SaqAI TTAA 3 cut(s) 59, 378, 502
Sau3AI GATC 1 cut(s) 137
Sau96I GGNCC 2 cut(s) 5, 263
SchI GAGTC 1 cut(s) 246
SetI ASST 9 cut(s) 59, 78, 129, 189, 219, 396, 429, 444, 470
SfaNI GCATC 2 cut(s) 37, 77
SfcI CTRYAG 1 cut(s) 486
SinI GGWCC 2 cut(s) 5, 263
SmiMI CAYNNNNRTG 2 cut(s) 207, 245
SpeI ACTAGT 1 cut(s) 244
SphI GCATGC 1 cut(s) 350
Sse9I AATT 4 cut(s) 60, 163, 322, 476
SspMI CTAG 2 cut(s) 245, 372
StyI CCWWGG 3 cut(s) 72, 196, 287
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 2 cut(s) 129, 219
TaqI TCGA 1 cut(s) 295
TasI AATT 4 cut(s) 60, 163, 322, 476
TatI WGTACW 2 cut(s) 249, 327
TfiI GAWTC 2 cut(s) 292, 367
Tru1I TTAA 3 cut(s) 59, 378, 502
Tru9I TTAA 3 cut(s) 59, 378, 502
TseFI GTSAC 3 cut(s) 238, 339, 427
Tsp45I GTSAC 3 cut(s) 238, 339, 427
TspDTI ATGAA 2 cut(s) 380, 470
TspGWI ACGGA 1 cut(s) 315
Van91I CCANNNNNTGG 2 cut(s) 78, 165
VpaK11BI GGWCC 2 cut(s) 5, 263
XapI RAATTY 1 cut(s) 476
XceI RCATGY 2 cut(s) 314, 350
XspI CTAG 2 cut(s) 245, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.