Rh7AG316300

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008415
Physical Location & Seq
Reverse (-)
35571318 .. 35572898
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG316300.1

Sequence Viewer

Length: 1149 bp
ATGGCTTCTCAATACTGCTTTGTTTTGGTTAATGTCATCATTCTCCTCTGCAGCTTGGGTCCGAACCTGAGTTTGGCAAAGGAAACAAAAGTCCATGGCTTGAAAATGAACGCAATTGATGGTTGCTGGAGATGGAATCCAAATTGGAGAAGCAACCGCCAGCAACTCGCAATGTGTTCGGTAGGATTTGCCGGAAAAATGAGCAACAACATAGGAAAAGATGTCATAAACTATGAAGTAACAGATCCCAGTGACAATGCATTAAATCCCCAACCAGGCACTTTGAGATATGGAGCTACTATGATCAAAGGCAAAAAATGGATCACAGTCCAGAGGGACATGAAAATTAAACTCGAGAAACCATTGCTCGTTAGTAGCTTCACTGCAATTGATGGAAGAGGTGCTAATGTCCACATACAAGGTAACGCGTGCTTGAGAGTGTTCAAAGCAACCAATGTTATCATACATGGCCTTCGAATCCACCACTGCAAATCGCAGCCACCGACCACGGTTATGGGTCCGAATGGAATAGTTTCACTAGGAGCGGTTGATGGAGACGCAATAAGGTTGGTTACTGCATCAAAGGTTTGGATAGACCACAATACACTATACGAGTGTGAGGATGGTCTTCTCGACGTTACTCGTGGATCAACCGATGTCACCGTCTCCAACAACTGGTTCAGAAACCAGGACAAGGTAATGCTTCTCGGCCATGATGATGGCTTCCTTCGGGACAAGAACATGAAGGTGACTGTAGTGTACAATCATTTTGGACCACTCTGCAATCAGAGAATGCCAAGGATTCGCCATGGATATGCACATGTCGTAAACAACTTGTACAGGGAATGGACACAGTATGCAATTGGGGGAAGCATGAACCCTAGTGTTAAAAGCGAAGCCAACCTCTTCATTGCACCAAAACAAAACAAAGAGATCACTTGGAGGCAGGACAGTGTTAACAACTCCTGGAAATTTTACTCTAAAGGCGATATTTTTGAAAATGGGGCTTCCTTCATCCAAACAGGTCAAGGTGGAGCCCAGCCACACTATAACATGGAACAAAATTTCCGAGTTGCCGACGCCAAATCTGTCCGGTCATTGACAAGGTCATCAGGTGCTCTAACATGCACCAAACAATCTAGATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

382

Amino Acids

42.65

Weight (kDa)

9.46

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 123 - 320 1.6e-26 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 675
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 157, 545
AclWI GGATC 3 cut(s) 239, 329, 655
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 2 cut(s) 971, 1063
AcyI GRCGYC 1 cut(s) 1080
AfaI GTAC 2 cut(s) 761, 839
AfiI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
AflIII ACRYGT 2 cut(s) 426, 820
AgsI TTSAA 3 cut(s) 103, 445, 998
AjnI CCWGG 3 cut(s) 274, 687, 965
AjuI GAANNNNNNNTTGG 4 cut(s) 56, 88, 662, 694
AloI GAACNNNNNNTCC 2 cut(s) 1050, 1082
AluBI AGCT 3 cut(s) 54, 296, 378
AluI AGCT 3 cut(s) 54, 296, 378
Alw21I GWGCWC 1 cut(s) 1120
Alw26I GTCTC 2 cut(s) 549, 670
AlwI GGATC 3 cut(s) 239, 329, 655
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 2 cut(s) 469, 709
ApeKI GCWGC 2 cut(s) 51, 496
ApoI RAATTY 2 cut(s) 971, 1063
Asp700I GAANNNNTTC 1 cut(s) 532
AspS9I GGNCC 3 cut(s) 59, 518, 773
AsuHPI GGTGA 2 cut(s) 652, 760
AsuII TTCGAA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 3 cut(s) 59, 518, 773
BanII GRGCYC 1 cut(s) 1039
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 1120
BbvI GCAGC 2 cut(s) 63, 508
BccI CCATC 6 cut(s) 113, 126, 386, 545, 617, 713
BcgI CGANNNNNNTGC 2 cut(s) 159, 193
BciT130I CCWGG 3 cut(s) 276, 689, 967
BclI TGATCA 1 cut(s) 303
BcoDI GTCTC 2 cut(s) 549, 670
BfaI CTAG 3 cut(s) 539, 882, 1140
BfmI CTRYAG 2 cut(s) 49, 753
BisI GCNGC 2 cut(s) 52, 497
BlsI GCNGC 2 cut(s) 53, 498
Bme1390I CCNGG 3 cut(s) 276, 689, 967
Bme18I GGWCC 3 cut(s) 59, 518, 773
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 3 cut(s) 59, 518, 773
BmiI GGNNCC 3 cut(s) 60, 519, 1036
BmrFI CCNGG 3 cut(s) 276, 689, 967
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 2 cut(s) 587, 1133
BmuI ACTGGG 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 620
BpmI CTGGAG 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 454
BsaHI GRCGYC 1 cut(s) 1080
BsaJI CCNNGG 4 cut(s) 94, 507, 797, 808
BsaWI WCCGGW 1 cut(s) 1092
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
Bse1I ACTGG 2 cut(s) 249, 680
Bse3DI GCAATG 3 cut(s) 177, 362, 909
BseBI CCWGG 3 cut(s) 276, 689, 967
BseDI CCNNGG 4 cut(s) 94, 507, 797, 808
BseGI GGATG 2 cut(s) 628, 1014
BseLI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
BseMI GCAATG 3 cut(s) 177, 362, 909
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 2 cut(s) 249, 680
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 63, 508
BseYI CCCAGC 1 cut(s) 1038
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 2 cut(s) 471, 711
BsiHKAI GWGCWC 1 cut(s) 1120
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 2 cut(s) 192, 1093
BslFI GGGAC 2 cut(s) 350, 746
BslI CCNNNNNNNGG 4 cut(s) 73, 275, 675, 694
BsmAI GTCTC 2 cut(s) 549, 670
BsmBI CGTCTC 2 cut(s) 549, 670
BsmFI GGGAC 2 cut(s) 350, 746
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 2 cut(s) 471, 711
BsoBI CYCGRG 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 1039, 1120
Bsp1407I TGTACA 2 cut(s) 759, 837
Bsp143I GATC 5 cut(s) 244, 303, 321, 647, 933
Bsp19I CCATGG 2 cut(s) 94, 808
BspACI CCGC 2 cut(s) 157, 545
BspANI GGCC 2 cut(s) 471, 711
BspCNI CTCAG 1 cut(s) 60
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 3 cut(s) 60, 519, 1036
BspMAI CTGCAG 1 cut(s) 53
BspPI GGATC 3 cut(s) 239, 329, 655
BspT104I TTCGAA 1 cut(s) 475
BsrBI CCGCTC 1 cut(s) 545
BsrDI GCAATG 3 cut(s) 177, 362, 909
BsrGI TGTACA 2 cut(s) 759, 837
BsrI ACTGG 2 cut(s) 249, 680
BssECI CCNNGG 4 cut(s) 94, 507, 797, 808
BssMI GATC 5 cut(s) 244, 303, 321, 647, 933
BssNI GRCGYC 1 cut(s) 1080
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 3 cut(s) 94, 797, 808
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 3 cut(s) 276, 689, 967
Bst4CI ACNGT 6 cut(s) 328, 511, 664, 754, 855, 953
Bst6I CTCTTC 2 cut(s) 391, 911
BstACI GRCGYC 1 cut(s) 1080
BstAUI TGTACA 2 cut(s) 759, 837
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 2 cut(s) 161, 430
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 3 cut(s) 94, 507, 808
BstF5I GGATG 2 cut(s) 628, 1014
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 5 cut(s) 247, 306, 324, 650, 936
BstMAI GTCTC 2 cut(s) 549, 670
BstMBI GATC 5 cut(s) 244, 303, 321, 647, 933
BstNI CCWGG 3 cut(s) 276, 689, 967
BstNSI RCATGY 2 cut(s) 824, 1128
BstSCI CCNGG 3 cut(s) 274, 687, 965
BstSFI CTRYAG 2 cut(s) 49, 753
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 63, 508
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 244
BstXI CCANNNNNNTGG 2 cut(s) 514, 719
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 471, 711
BtgI CCRYGG 3 cut(s) 94, 507, 808
BtsCI GGATG 2 cut(s) 628, 1014
BtsI GCAGTG 2 cut(s) 381, 484
BtsIMutI CAGTG 4 cut(s) 256, 381, 484, 958
Cac8I GCNNGC 2 cut(s) 161, 430
Cfr13I GGNCC 3 cut(s) 59, 518, 773
CseI GACGC 2 cut(s) 566, 1088
Csp6I GTAC 2 cut(s) 760, 838
CviQI GTAC 2 cut(s) 760, 838
DdeI CTNAG 1 cut(s) 68
DpnI GATC 5 cut(s) 246, 305, 323, 649, 935
DpnII GATC 5 cut(s) 244, 303, 321, 647, 933
EaeI YGGCCR 1 cut(s) 709
Eam1104I CTCTTC 2 cut(s) 391, 911
EarI CTCTTC 2 cut(s) 391, 911
Eco130I CCWWGG 3 cut(s) 94, 797, 808
Eco24I GRGCYC 1 cut(s) 1039
Eco47I GGWCC 3 cut(s) 59, 518, 773
Eco88I CYCGRG 1 cut(s) 353
EcoRII CCWGG 3 cut(s) 274, 687, 965
EcoT14I CCWWGG 3 cut(s) 94, 797, 808
EcoT22I ATGCAT 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 1039
ErhI CCWWGG 3 cut(s) 94, 797, 808
Esp3I CGTCTC 2 cut(s) 549, 670
FaqI GGGAC 2 cut(s) 350, 746
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 2 cut(s) 52, 497
FokI GGATG 2 cut(s) 635, 1001
FriOI GRGCYC 1 cut(s) 1039
Fsp4HI GCNGC 2 cut(s) 52, 497
FspBI CTAG 3 cut(s) 539, 882, 1140
GluI GCNGC 2 cut(s) 52, 497
GsaI CCCAGC 1 cut(s) 1042
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 2 cut(s) 471, 711
HapII CCGG 2 cut(s) 192, 1093
HgaI GACGC 2 cut(s) 566, 1088
Hin1I GRCGYC 1 cut(s) 1080
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HinfI GANTC 3 cut(s) 136, 477, 802
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 2 cut(s) 192, 1093
HphI GGTGA 2 cut(s) 652, 760
Hpy166II GTNNAC 4 cut(s) 412, 760, 829, 958
Hpy188I TCNGA 5 cut(s) 63, 522, 683, 789, 1070
Hpy188III TCNNGA 5 cut(s) 331, 355, 632, 731, 1140
Hpy8I GTNNAC 4 cut(s) 412, 760, 829, 958
Hpy99I CGWCG 2 cut(s) 638, 1082
HpyAV CCTTC 4 cut(s) 482, 737, 739, 1021
HpyCH4III ACNGT 6 cut(s) 328, 511, 664, 754, 855, 953
HpyCH4IV ACGT 1 cut(s) 636
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 636
Hsp92I GRCGYC 1 cut(s) 1080
Ksp22I TGATCA 1 cut(s) 303
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 5 cut(s) 244, 303, 321, 647, 933
LmnI GCTCC 3 cut(s) 293, 542, 1034
Lsp1109I GCAGC 2 cut(s) 63, 508
LweI GCATC 2 cut(s) 587, 1133
MaeI CTAG 3 cut(s) 539, 882, 1140
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 7 cut(s) 238, 251, 422, 571, 637, 658, 748
MalI GATC 5 cut(s) 246, 305, 323, 649, 935
MbiI CCGCTC 1 cut(s) 545
MboI GATC 5 cut(s) 244, 303, 321, 647, 933
MboII GAAGA 3 cut(s) 408, 620, 898
MfeI CAATTG 3 cut(s) 114, 387, 861
MflI RGATCY 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 1039, 1120
MluCI AATT 7 cut(s) 114, 142, 345, 387, 861, 971, 1063
MluI ACGCGT 1 cut(s) 426
MmeI TCCRAC 1 cut(s) 693
MnlI CCTC 6 cut(s) 56, 327, 392, 613, 914, 936
Mph1103I ATGCAT 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 532
MseI TTAA 5 cut(s) 30, 263, 348, 888, 957
MslI CAYNNNNRTG 4 cut(s) 512, 717, 746, 813
MspI CCGG 2 cut(s) 192, 1093
MspR9I CCNGG 3 cut(s) 276, 689, 967
MunI CAATTG 3 cut(s) 114, 387, 861
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 3 cut(s) 276, 689, 967
MvnI CGCG 1 cut(s) 428
NcoI CCATGG 2 cut(s) 94, 808
NdeII GATC 5 cut(s) 244, 303, 321, 647, 933
NlaIV GGNNCC 3 cut(s) 60, 519, 1036
NmeAIII GCCGAG 1 cut(s) 687
NmuCI GTSAC 3 cut(s) 251, 658, 748
NsiI ATGCAT 1 cut(s) 262
NspI RCATGY 2 cut(s) 824, 1128
NspV TTCGAA 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 353
PciI ACATGT 1 cut(s) 820
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PctI GAATGC 1 cut(s) 798
PdmI GAANNNNTTC 1 cut(s) 532
PfeI GAWTC 3 cut(s) 136, 477, 802
PflFI GACNNNGTC 1 cut(s) 1105
PflMI CCANNNNNTGG 1 cut(s) 675
PfoI TCCNGGA 1 cut(s) 965
PkrI GCNGC 2 cut(s) 53, 498
PscI ACATGT 1 cut(s) 820
Psp6I CCWGG 3 cut(s) 274, 687, 965
PspFI CCCAGC 1 cut(s) 1038
PspGI CCWGG 3 cut(s) 274, 687, 965
PspN4I GGNNCC 3 cut(s) 60, 519, 1036
PspPI GGNCC 3 cut(s) 59, 518, 773
PstI CTGCAG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 244
PsyI GACNNNGTC 1 cut(s) 1105
RsaI GTAC 2 cut(s) 761, 839
RsaNI GTAC 2 cut(s) 760, 838
RseI CAYNNNNRTG 4 cut(s) 512, 717, 746, 813
SaqAI TTAA 5 cut(s) 30, 263, 348, 888, 957
SatI GCNGC 2 cut(s) 52, 497
Sau3AI GATC 5 cut(s) 244, 303, 321, 647, 933
Sau96I GGNCC 3 cut(s) 59, 518, 773
ScrFI CCNGG 3 cut(s) 276, 689, 967
SduI GDGCHC 2 cut(s) 1039, 1120
SfaNI GCATC 2 cut(s) 587, 1133
SfcI CTRYAG 2 cut(s) 49, 753
Sfr274I CTCGAG 1 cut(s) 353
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 3 cut(s) 59, 518, 773
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 4 cut(s) 512, 717, 746, 813
SmlI CTYRAG 2 cut(s) 353, 433
SmoI CTYRAG 2 cut(s) 353, 433
Sse9I AATT 7 cut(s) 114, 142, 345, 387, 861, 971, 1063
SsiI CCGC 2 cut(s) 157, 545
SspMI CTAG 3 cut(s) 539, 882, 1140
StyD4I CCNGG 3 cut(s) 274, 687, 965
StyI CCWWGG 3 cut(s) 94, 797, 808
TaaI ACNGT 6 cut(s) 328, 511, 664, 754, 855, 953
TaiI ACGT 1 cut(s) 639
TaqI TCGA 3 cut(s) 354, 475, 633
TasI AATT 7 cut(s) 114, 142, 345, 387, 861, 971, 1063
TatI WGTACW 2 cut(s) 759, 837
TfiI GAWTC 3 cut(s) 136, 477, 802
Tru1I TTAA 5 cut(s) 30, 263, 348, 888, 957
Tru9I TTAA 5 cut(s) 30, 263, 348, 888, 957
TscAI CASTG 4 cut(s) 256, 388, 491, 958
TseFI GTSAC 3 cut(s) 251, 658, 748
TseI GCWGC 2 cut(s) 51, 496
Tsp45I GTSAC 3 cut(s) 251, 658, 748
TspDTI ATGAA 7 cut(s) 122, 249, 356, 758, 890, 898, 1003
TspRI CASTG 4 cut(s) 256, 388, 491, 958
Tth111I GACNNNGTC 1 cut(s) 1105
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 3 cut(s) 59, 518, 773
XapI RAATTY 2 cut(s) 971, 1063
XbaI TCTAGA 1 cut(s) 1139
XceI RCATGY 2 cut(s) 824, 1128
XhoI CTCGAG 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 532
XspI CTAG 3 cut(s) 539, 882, 1140
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.