FvH4_5g28690

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008936
Physical Location & Seq
Forward (+)
19863670 .. 19871514
7845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28690.t1

Sequence Viewer

Length: 1059 bp
ATGGCGACTCAGAGTCAGAGCCCTCCGATCAGCTGGAGAGACTCGTACAGAGGCATGTCCTCTGATAACATCAAGGGTTTGGTCCTTGCTTTGTCTTCAAGCTTATTTATCGGTGCTAGTTTCATAGTCAAGAAAAAGGGCTTGAAGAAGGCCGGCGCTTCTGGCATCCGAGCAGGGGTTGGCGGATATTCATACCTTTACGAGCCACTTTGGTGGGTGGGCATGATCACAATGATTGTAGGAGAAGTTGCTAATTTTGCGGCTTATGCATTTGCACCAGCTATACTAGTAACTCCTCTTGGCGCTCTCAGCATAATTATCAGTGCTGTGCTTGCACATATTATTCTACGGGAAAGGCTGCATATCTTTGGGATTCTTGGTTGTGCTTTATGTGTTGTGGGTTCTACAACAATTGTTTTACATGCGCCTCAAGAACGTGAGATTGAATCTGTAGCCGAAGTTTGGGCTCTTGCCATGGAACCAGGTTTTCTATTGTATGCGGCTCTTGTCATAACTGCAGTGTTTATACTTATATTCCACGTCATTCCGCAATATGGCCAGACACATATTATGGTTTACATTGGAGTTTGTTCGCTTGTAGGTTCCTTGTCGGTCATGAGTGTTAAAGCACTTGGAATTGCTCTGAAGTTGACGTTATCCGGAATGAATCAACTAGTCTATCCTCAAACTTGGGTCTTCACTTTAGTTGTAATTACTTGTGTGCTCACCCAAATGAATTACTTGAACAAGGCACTTGATACATTCAACACAGCCGTGGTATCTCCTATATACTATGTTATGTTCACATCACTAACTATTCTGGCTAGTGTGATCATGTTTAAGGATTGGGACAGACAGAGTCCAACACAAGTTATCACAGAAATGTGTGGTTTTGTGACTATACTTGGAGGAACTTTTCTTCTTCACAGAACAAAGGATATGACTGACGGTCTGACAACATCAATGTCCTCTTTGCGACTGTCTAAGCATGCAGAGGAGGATGGAATGGATGGGGCTGAAGGCATCCCGCTCAGGCGGCAGGATAACTCAAGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

353

Amino Acids

38.26

Weight (kDa)

8.22

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 22 - 314 1.5e-135 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1030
AccIII TCCGGA 1 cut(s) 659
AciI CCGC 6 cut(s) 183, 260, 500, 548, 1028, 1036
AcoI YGGCCR 1 cut(s) 556
AcuI CTGAAG 2 cut(s) 665, 1038
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 175, 462, 554
AgsI TTSAA 5 cut(s) 99, 145, 446, 745, 766
AhdI GACNNNNNGTC 2 cut(s) 12, 948
AhlI ACTAGT 2 cut(s) 286, 673
AjiI CACGTC 1 cut(s) 541
AjnI CCWGG 1 cut(s) 481
AleI CACNNNNGTG 2 cut(s) 211, 773
AluBI AGCT 3 cut(s) 33, 102, 281
AluI AGCT 3 cut(s) 33, 102, 281
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 1 cut(s) 33
Aor13HI TCCGGA 1 cut(s) 659
AoxI GGCC 2 cut(s) 150, 556
ApeKI GCWGC 1 cut(s) 358
AspLEI GCGC 3 cut(s) 158, 305, 427
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 718
AvaII GGWCC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 558
BanII GRGCYC 2 cut(s) 23, 469
BbsI GAAGAC 2 cut(s) 87, 688
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 1 cut(s) 345
BccI CCATC 2 cut(s) 995, 1004
BceAI ACGGC 1 cut(s) 758
BciT130I CCWGG 1 cut(s) 483
BclI TGATCA 2 cut(s) 225, 831
BcoDI GTCTC 1 cut(s) 33
BcuI ACTAGT 2 cut(s) 286, 673
BfaI CTAG 4 cut(s) 117, 287, 674, 825
BfmI CTRYAG 2 cut(s) 450, 516
BfoI RGCGCY 2 cut(s) 159, 306
BisI GCNGC 4 cut(s) 261, 359, 501, 1037
BlsI GCNGC 4 cut(s) 262, 360, 502, 1038
Bme1390I CCNGG 1 cut(s) 483
Bme18I GGWCC 1 cut(s) 82
BmeRI GACNNNNNGTC 2 cut(s) 12, 948
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 480, 604
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 174, 1032
BpiI GAAGAC 2 cut(s) 87, 688
BpmI CTGGAG 1 cut(s) 55
Bpu10I CCTNAGC 1 cut(s) 1031
BpuEI CTTGAG 2 cut(s) 414, 1033
BsaJI CCNNGG 2 cut(s) 474, 774
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 462, 554
Bse118I RCCGGY 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 659
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 2 cut(s) 474, 774
BseGI GGATG 4 cut(s) 165, 1006, 1015, 1023
BseLI CCNNNNNNNGG 3 cut(s) 175, 462, 554
BseMII CTCAG 3 cut(s) 23, 322, 1045
BseRI GAGGAG 2 cut(s) 285, 1010
BseXI GCAGC 1 cut(s) 345
BshFI GGCC 2 cut(s) 152, 558
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 2 cut(s) 153, 660
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 3 cut(s) 175, 462, 554
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 863
BsnI GGCC 2 cut(s) 152, 558
Bsp1286I GDGCHC 3 cut(s) 23, 469, 726
Bsp13I TCCGGA 1 cut(s) 659
Bsp143I GATC 4 cut(s) 27, 225, 831, 1052
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 6 cut(s) 183, 260, 500, 548, 1028, 1036
BspANI GGCC 2 cut(s) 152, 558
BspCNI CTCAG 3 cut(s) 22, 321, 1044
BspEI TCCGGA 1 cut(s) 659
BspHI TCATGA 2 cut(s) 615, 1055
BspLI GGNNCC 2 cut(s) 480, 604
BspMAI CTGCAG 1 cut(s) 520
BsrBI CCGCTC 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 474, 774
BssMI GATC 4 cut(s) 27, 225, 831, 1052
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 2 cut(s) 950, 981
BstC8I GCNNGC 3 cut(s) 154, 333, 990
BstDEI CTNAG 4 cut(s) 9, 308, 984, 1031
BstDSI CCRYGG 2 cut(s) 474, 774
BstF5I GGATG 4 cut(s) 165, 1006, 1015, 1023
BstH2I RGCGCY 2 cut(s) 159, 306
BstHHI GCGC 3 cut(s) 158, 305, 427
BstKTI GATC 4 cut(s) 30, 228, 834, 1055
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 4 cut(s) 27, 225, 831, 1052
BstMWI GCNNNNNNNGC 6 cut(s) 162, 257, 266, 309, 332, 1036
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 3 cut(s) 58, 425, 992
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 2 cut(s) 450, 516
BstV1I GCAGC 1 cut(s) 345
BstV2I GAAGAC 2 cut(s) 87, 688
BstXI CCANNNNNNTGG 1 cut(s) 213
BsuRI GGCC 2 cut(s) 152, 558
BtgI CCRYGG 2 cut(s) 474, 774
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 4 cut(s) 165, 1006, 1015, 1023
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 328, 525
Cac8I GCNNGC 3 cut(s) 154, 333, 990
CciI TCATGA 2 cut(s) 615, 1055
CfoI GCGC 3 cut(s) 158, 305, 427
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 1 cut(s) 46
CviAII CATG 8 cut(s) 55, 223, 422, 475, 616, 835, 989, 1056
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 4 cut(s) 9, 308, 984, 1031
DpnI GATC 4 cut(s) 29, 227, 833, 1054
DpnII GATC 4 cut(s) 27, 225, 831, 1052
DriI GACNNNNNGTC 2 cut(s) 12, 948
EaeI YGGCCR 1 cut(s) 556
Eam1105I GACNNNNNGTC 2 cut(s) 12, 948
EciI GGCGGA 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 474
Eco24I GRGCYC 2 cut(s) 23, 469
Eco47I GGWCC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 665, 1038
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 2 cut(s) 23, 469
ErhI CCWWGG 1 cut(s) 474
FaeI CATG 8 cut(s) 58, 226, 425, 478, 619, 838, 992, 1059
FaqI GGGAC 1 cut(s) 863
FatI CATG 8 cut(s) 54, 222, 421, 474, 615, 834, 988, 1055
FauI CCCGC 1 cut(s) 1035
FbaI TGATCA 2 cut(s) 225, 831
Fnu4HI GCNGC 4 cut(s) 261, 359, 501, 1037
FokI GGATG 4 cut(s) 152, 1010, 1013, 1022
FriOI GRGCYC 2 cut(s) 23, 469
Fsp4HI GCNGC 4 cut(s) 261, 359, 501, 1037
FspBI CTAG 4 cut(s) 117, 287, 674, 825
GlaI GCGC 3 cut(s) 157, 304, 426
GluI GCNGC 4 cut(s) 261, 359, 501, 1037
GsuI CTGGAG 1 cut(s) 55
HaeII RGCGCY 2 cut(s) 159, 306
HaeIII GGCC 2 cut(s) 152, 558
HapII CCGG 2 cut(s) 153, 660
HhaI GCGC 3 cut(s) 158, 305, 427
Hin1II CATG 8 cut(s) 58, 226, 425, 478, 619, 838, 992, 1059
Hin6I GCGC 3 cut(s) 156, 303, 425
HinP1I GCGC 3 cut(s) 156, 303, 425
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 100
HinfI GANTC 7 cut(s) 7, 13, 41, 373, 446, 667, 859
HpaII CCGG 2 cut(s) 153, 660
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 3 cut(s) 577, 651, 804
Hpy188I TCNGA 7 cut(s) 12, 18, 27, 64, 170, 645, 954
Hpy188III TCNNGA 6 cut(s) 130, 431, 616, 660, 1050, 1056
Hpy8I GTNNAC 3 cut(s) 577, 651, 804
HpyAV CCTTC 2 cut(s) 142, 1013
HpyCH4III ACNGT 2 cut(s) 950, 981
HpyCH4IV ACGT 3 cut(s) 436, 540, 653
HpyCH4V TGCA 6 cut(s) 269, 275, 335, 361, 518, 992
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 257, 266, 309, 332, 1036
HpyF3I CTNAG 4 cut(s) 9, 308, 984, 1031
HpySE526I ACGT 3 cut(s) 436, 540, 653
Hsp92II CATG 8 cut(s) 58, 226, 425, 478, 619, 838, 992, 1059
HspAI GCGC 3 cut(s) 156, 303, 425
Kpn2I TCCGGA 1 cut(s) 659
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 2 cut(s) 225, 831
Kzo9I GATC 4 cut(s) 27, 225, 831, 1052
Lsp1109I GCAGC 1 cut(s) 345
LweI GCATC 2 cut(s) 174, 1032
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 4 cut(s) 117, 287, 674, 825
MaeII ACGT 3 cut(s) 436, 540, 653
MaeIII GTNAC 2 cut(s) 289, 895
MalI GATC 4 cut(s) 29, 227, 833, 1054
MbiI CCGCTC 1 cut(s) 1030
MboI GATC 4 cut(s) 27, 225, 831, 1052
MboII GAAGA 5 cut(s) 87, 157, 688, 911, 914
MfeI CAATTG 1 cut(s) 411
MhlI GDGCHC 3 cut(s) 23, 469, 726
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 6 cut(s) 253, 315, 411, 636, 711, 736
MluNI TGGCCA 1 cut(s) 558
MlyI GAGTC 3 cut(s) 22, 35, 868
MmeI TCCRAC 1 cut(s) 887
Mox20I TGGCCA 1 cut(s) 558
Mph1103I ATGCAT 1 cut(s) 271
MroI TCCGGA 1 cut(s) 659
MroNI GCCGGC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 558
MseI TTAA 2 cut(s) 624, 840
MslI CAYNNNNRTG 4 cut(s) 211, 731, 773, 881
Msp20I TGGCCA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 2 cut(s) 153, 660
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 411
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 6 cut(s) 162, 257, 266, 309, 332, 1036
NaeI GCCGGC 1 cut(s) 154
NcoI CCATGG 1 cut(s) 474
NdeII GATC 4 cut(s) 27, 225, 831, 1052
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 8 cut(s) 58, 226, 425, 478, 619, 838, 992, 1059
NlaIV GGNNCC 2 cut(s) 480, 604
NmuCI GTSAC 1 cut(s) 895
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 3 cut(s) 58, 425, 992
OliI CACNNNNGTG 2 cut(s) 211, 773
PaeI GCATGC 1 cut(s) 992
PagI TCATGA 2 cut(s) 615, 1055
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 3 cut(s) 373, 446, 667
PflFI GACNNNGTC 1 cut(s) 858
PkrI GCNGC 4 cut(s) 262, 360, 502, 1038
PleI GAGTC 3 cut(s) 21, 35, 867
PpsI GAGTC 3 cut(s) 21, 35, 867
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 480, 604
PspPI GGNCC 1 cut(s) 82
PstI CTGCAG 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 858
PvuII CAGCTG 1 cut(s) 33
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 4 cut(s) 211, 731, 773, 881
SaqAI TTAA 2 cut(s) 624, 840
SatI GCNGC 4 cut(s) 261, 359, 501, 1037
Sau3AI GATC 4 cut(s) 27, 225, 831, 1052
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 3 cut(s) 22, 35, 868
ScrFI CCNGG 1 cut(s) 483
SduI GDGCHC 3 cut(s) 23, 469, 726
SetI ASST 9 cut(s) 35, 104, 198, 283, 439, 487, 543, 604, 656
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 2 cut(s) 174, 1032
SfcI CTRYAG 2 cut(s) 450, 516
SinI GGWCC 1 cut(s) 82
SmiMI CAYNNNNRTG 4 cut(s) 211, 731, 773, 881
SmlI CTYRAG 2 cut(s) 429, 1048
SmoI CTYRAG 2 cut(s) 429, 1048
SpeI ACTAGT 2 cut(s) 286, 673
SphI GCATGC 1 cut(s) 992
Sse9I AATT 6 cut(s) 253, 315, 411, 636, 711, 736
SsiI CCGC 6 cut(s) 183, 260, 500, 548, 1028, 1036
SspMI CTAG 4 cut(s) 117, 287, 674, 825
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 2 cut(s) 950, 981
TaiI ACGT 3 cut(s) 439, 543, 656
TaqII GACCGA 1 cut(s) 601
TasI AATT 6 cut(s) 253, 315, 411, 636, 711, 736
TauI GCSGC 3 cut(s) 263, 503, 1039
TfiI GAWTC 3 cut(s) 373, 446, 667
Tru1I TTAA 2 cut(s) 624, 840
Tru9I TTAA 2 cut(s) 624, 840
TscAI CASTG 2 cut(s) 328, 525
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 4 cut(s) 112, 180, 680, 749
TspRI CASTG 2 cut(s) 328, 525
Tth111I GACNNNGTC 1 cut(s) 858
VpaK11BI GGWCC 1 cut(s) 82
XceI RCATGY 3 cut(s) 58, 425, 992
XspI CTAG 4 cut(s) 117, 287, 674, 825
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.