Rh7DG336900

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008936
Physical Location & Seq
Reverse (-)
44169190 .. 44177661
8472 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG336900.1

Sequence Viewer

Length: 786 bp
ATGGCGACTCAGGTTCAGAGCCCGATGAGCTGGAGAGACTCGTACAGAGGCATGTCCTCTGACAACATTAAGGGTTTGGTTCTTGCTTTGTCTTCAAGCTTGTTTATTGGTGCTAGTTTCATAGTCAAGAAAAAGGGCTTGAAGAAGGCTGGGGCTTCTGGCATCCGAGCAGGAGTGGGAGGATATTCATACCTATATGAGCCACTTTGGTGGGTGGGCATGATAACAATGATTGTAGGAGAAGTTGCTAATTTTGCGGCTTATGCATTTGCGCCAGCTATACTAGTAACTCCTCTTGGCGCTCTCAGCATAATTATCAGTGCTGTGCTTGCACATATTATTTTAAGGGAGAAGCTGCATATCTTTGGGATTCTTGGTTGTGCTTTATGTGTTGTTGGTTCTACAACAATTGTTTTACATGCTCCTCAAGAACGTGAGATTGAATCTGTAACAGAAGTTTGGGCTCTTGCCATGGAACCAGGTTTTCTATTTTATGCAGCTCTTGTCATAACTGCTGTTTTTATACTTATATTCCACTTCATCCCGCAATATGGCCAGACACACATAATGGTTTACATTGGAGTTTGTTCGCTTGTAGGTTCCTTGTCGGTCATGAGTGTTAAAGCACTTGGAATTGCTCTGAAGTTGACGTTATCCGGAATGAATCAACTAGTCTATCCCCAAACTTGGGTCTTCACTTTAGTTGTAATTACTTGTGTGCTCACCCAAATGAATTACTTGAACAAGGTAATTACTAGTTCTTTTCTGTTCGTTTTTTTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

261

Amino Acids

28.36

Weight (kDa)

9.03

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 21 - 251 6e-104 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 656
AciI CCGC 2 cut(s) 257, 545
AcoI YGGCCR 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 551, 687, 688
AgsI TTSAA 4 cut(s) 96, 142, 443, 742
AhlI ACTAGT 3 cut(s) 283, 670, 755
AjnI CCWGG 1 cut(s) 478
AleI CACNNNNGTG 1 cut(s) 208
AluBI AGCT 5 cut(s) 30, 99, 278, 355, 500
AluI AGCT 5 cut(s) 30, 99, 278, 355, 500
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 656
AoxI GGCC 1 cut(s) 553
ApeKI GCWGC 2 cut(s) 355, 497
AspLEI GCGC 2 cut(s) 274, 302
AsuHPI GGTGA 1 cut(s) 715
BalI TGGCCA 1 cut(s) 555
BanII GRGCYC 2 cut(s) 23, 466
BbsI GAAGAC 2 cut(s) 84, 685
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 342, 509
BciT130I CCWGG 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 30
BcuI ACTAGT 3 cut(s) 283, 670, 755
BfaI CTAG 4 cut(s) 114, 284, 671, 756
BfoI RGCGCY 1 cut(s) 303
BisI GCNGC 3 cut(s) 258, 356, 498
BlsI GCNGC 3 cut(s) 259, 357, 499
Bme1390I CCNGG 1 cut(s) 480
BmiI GGNNCC 2 cut(s) 477, 601
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 1 cut(s) 171
BpiI GAAGAC 2 cut(s) 84, 685
BpmI CTGGAG 1 cut(s) 52
BpuEI CTTGAG 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 471
BsaWI WCCGGW 1 cut(s) 656
Bsc4I CCNNNNNNNGG 3 cut(s) 551, 687, 688
BseAI TCCGGA 1 cut(s) 656
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 2 cut(s) 162, 540
BseLI CCNNNNNNNGG 3 cut(s) 551, 687, 688
BseMII CTCAG 2 cut(s) 23, 319
BseRI GAGGAG 2 cut(s) 282, 414
BseXI GCAGC 2 cut(s) 342, 509
BseYI CCCAGC 1 cut(s) 149
BshFI GGCC 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 723
BsiSI CCGG 1 cut(s) 657
BslI CCNNNNNNNGG 3 cut(s) 551, 687, 688
BsmAI GTCTC 1 cut(s) 30
BsnI GGCC 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 23, 466, 723
Bsp13I TCCGGA 1 cut(s) 656
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 2 cut(s) 257, 545
BspANI GGCC 1 cut(s) 555
BspCNI CTCAG 2 cut(s) 22, 318
BspEI TCCGGA 1 cut(s) 656
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 477, 601
BssECI CCNNGG 1 cut(s) 471
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 276, 330
BstDEI CTNAG 2 cut(s) 9, 305
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 162, 540
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 2 cut(s) 274, 302
BstMAI GTCTC 1 cut(s) 30
BstMWI GCNNNNNNNGC 5 cut(s) 27, 254, 263, 306, 329
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 2 cut(s) 55, 422
BstSCI CCNGG 1 cut(s) 478
BstV1I GCAGC 2 cut(s) 342, 509
BstV2I GAAGAC 2 cut(s) 84, 685
BstXI CCANNNNNNTGG 1 cut(s) 210
BsuRI GGCC 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 2 cut(s) 162, 540
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 276, 330
CciI TCATGA 1 cut(s) 612
CfoI GCGC 2 cut(s) 274, 302
CsiI ACCWGGT 1 cut(s) 478
Csp6I GTAC 1 cut(s) 43
CviAII CATG 5 cut(s) 52, 220, 419, 472, 613
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 2 cut(s) 9, 305
EaeI YGGCCR 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 23, 466
Eco57I CTGAAG 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 2 cut(s) 23, 466
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 55, 223, 422, 475, 616
FatI CATG 5 cut(s) 51, 219, 418, 471, 612
FauI CCCGC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 258, 356, 498
FokI GGATG 2 cut(s) 149, 527
FriOI GRGCYC 2 cut(s) 23, 466
Fsp4HI GCNGC 3 cut(s) 258, 356, 498
FspBI CTAG 4 cut(s) 114, 284, 671, 756
GlaI GCGC 2 cut(s) 273, 301
GluI GCNGC 3 cut(s) 258, 356, 498
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 52
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 1 cut(s) 555
HapII CCGG 1 cut(s) 657
HhaI GCGC 2 cut(s) 274, 302
Hin1II CATG 5 cut(s) 55, 223, 422, 475, 616
Hin6I GCGC 2 cut(s) 272, 300
HinP1I GCGC 2 cut(s) 272, 300
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 5 cut(s) 7, 38, 370, 443, 664
HpaII CCGG 1 cut(s) 657
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 2 cut(s) 574, 648
Hpy188I TCNGA 4 cut(s) 18, 61, 167, 642
Hpy188III TCNNGA 4 cut(s) 127, 428, 613, 657
Hpy8I GTNNAC 2 cut(s) 574, 648
HpyAV CCTTC 1 cut(s) 139
HpyCH4IV ACGT 2 cut(s) 433, 650
HpyCH4V TGCA 4 cut(s) 266, 332, 358, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 254, 263, 306, 329
HpyF3I CTNAG 2 cut(s) 9, 305
HpySE526I ACGT 2 cut(s) 433, 650
Hsp92II CATG 5 cut(s) 55, 223, 422, 475, 616
HspAI GCGC 2 cut(s) 272, 300
Kpn2I TCCGGA 1 cut(s) 656
LmnI GCTCC 1 cut(s) 427
LpnPI CCDG 9 cut(s) 16, 135, 144, 156, 288, 465, 492, 569, 670
Lsp1109I GCAGC 2 cut(s) 342, 509
LweI GCATC 1 cut(s) 171
MabI ACCWGGT 1 cut(s) 478
MaeI CTAG 4 cut(s) 114, 284, 671, 756
MaeII ACGT 2 cut(s) 433, 650
MaeIII GTNAC 2 cut(s) 286, 448
MboII GAAGA 3 cut(s) 84, 154, 685
MfeI CAATTG 1 cut(s) 408
MhlI GDGCHC 3 cut(s) 23, 466, 723
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 7 cut(s) 250, 312, 408, 633, 708, 733, 750
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 5 cut(s) 41, 67, 173, 303, 435
Mox20I TGGCCA 1 cut(s) 555
Mph1103I ATGCAT 1 cut(s) 268
MroI TCCGGA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 555
MseI TTAA 4 cut(s) 69, 344, 621, 784
MslI CAYNNNNRTG 2 cut(s) 208, 728
Msp20I TGGCCA 1 cut(s) 555
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 480
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 5 cut(s) 27, 254, 263, 306, 329
NcoI CCATGG 1 cut(s) 471
NlaIII CATG 5 cut(s) 55, 223, 422, 475, 616
NlaIV GGNNCC 2 cut(s) 477, 601
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 2 cut(s) 55, 422
OliI CACNNNNGTG 1 cut(s) 208
PagI TCATGA 1 cut(s) 612
PfeI GAWTC 3 cut(s) 370, 443, 664
PkrI GCNGC 3 cut(s) 259, 357, 499
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp6I CCWGG 1 cut(s) 478
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 2 cut(s) 477, 601
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 2 cut(s) 208, 728
SaqAI TTAA 4 cut(s) 69, 344, 621, 784
SatI GCNGC 3 cut(s) 258, 356, 498
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 1 cut(s) 480
SduI GDGCHC 3 cut(s) 23, 466, 723
SexAI ACCWGGT 1 cut(s) 478
SfaNI GCATC 1 cut(s) 171
SmiMI CAYNNNNRTG 2 cut(s) 208, 728
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
SpeI ACTAGT 3 cut(s) 283, 670, 755
Sse9I AATT 7 cut(s) 250, 312, 408, 633, 708, 733, 750
SsiI CCGC 2 cut(s) 257, 545
SspMI CTAG 4 cut(s) 114, 284, 671, 756
StyD4I CCNGG 1 cut(s) 478
StyI CCWWGG 1 cut(s) 471
TaiI ACGT 2 cut(s) 436, 653
TaqII GACCGA 1 cut(s) 598
TasI AATT 7 cut(s) 250, 312, 408, 633, 708, 733, 750
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 3 cut(s) 370, 443, 664
Tru1I TTAA 4 cut(s) 69, 344, 621, 784
Tru9I TTAA 4 cut(s) 69, 344, 621, 784
TscAI CASTG 1 cut(s) 325
TseI GCWGC 2 cut(s) 355, 497
TspDTI ATGAA 5 cut(s) 109, 177, 529, 677, 746
TspRI CASTG 1 cut(s) 325
XceI RCATGY 2 cut(s) 55, 422
XspI CTAG 4 cut(s) 114, 284, 671, 756
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.