MD08G1240300.v1.1

Acts as a Mg(2 ) transporter. Can also transport other divalent cations such as Fe(2 ), Sr(2 ), Ba(2 ), Mn(2 ) and Co(2 ) but to a much less extent than Mg(2 )

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008936
Physical Location & Seq
Reverse (-)
30650089 .. 30655237
5149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1240300.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGCGACCGAGGGTCAGAGCCCCCCGATGAGCTGGAGAGACTCGTACAAGGGGATGTCCTCTGACAACATCAACGGTTTGATTCTTGCTTTGTCTTCAAGTTTGTTTATCGGTGCTAGTTTCATTGTGAAGAAGAAGGGGTTGAAAAAGGCCGGGGCTTCTGGCATCAGAGCAGGAGTCGGAGGATATTCTTACCTATACGAGCCACTTTGGTGGGTGGGCATGATAACGATGATTGTTGGGGAAATTGCTAATTTTGCGGCTTATGCTTTTGCACCAGCTATACTAGTAACTCCTCTTGGTGCTCTCAGCATAATTATCAGTGCTGTGCTTGCACATATTATTTTACGTGAGAGGCTTCATATTTTTGGTGTTCTTGGTTGCGCTTTGTGTGTTGTGGGTTCTACAACGATTGTTTTACATGCTCCTCAAGAACGTATGATTGAATCTGTTACAGAAGTTTGGGATCTTGCCATGGAACCAGGTTTTCTCTTGTATGCAGCTTTGGTCATAACTGCTGTGTTTATACTTATATTCCACTTTATCCCGCAATATGGCCAGACACACATAATGGTCTACATTGGAGTTTGTTCCCTTGTTGGTTCCTTATCGGTCATGAGTGTTAAAGCACTTGGAATTGCTTTGAAGTTGACATTATCGGGCATGAATCAGCTAGTTTACCCCCAAACTTGGGTCTTCACTTTAGTTGTCATTTCTTGTGTGCTTACCCAAATGAATTATTTGAATAAGGCACTTGATACTTTCAACACAGCTGTGGTATCTCCTATATACTATGTTATGTTCACATCACTAACTATTCTGGCTAGTGTTATCATGTTCAAGGATTGGGACAGACAGAGTCCAACTCAGGTTGTCACCGAAATGTGTGGGTTTGTAACCATCCTTGGAGGAACTTTTCTTCTTCACAGAACGAAGGATATGGTTGATGGTTTGCCAACATCAATGTCCTCCGTGCGACTATCTAAGCATGCAGATGAAGATGGAATCGATGGTTCTGAAGGCATCCCTCTTAGACGGCAGAATAATTCCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mg_trans_NIPA

Protein Analysis

352

Amino Acids

38.15

Weight (kDa)

7.04

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_trans_NIPA PF05653 22 - 314 3.7e-135 Magnesium transporter NIPA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 576
AciI CCGC 2 cut(s) 260, 548
AclWI GGATC 1 cut(s) 474
AcoI YGGCCR 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 1038
AfaI GTAC 1 cut(s) 47
AfiI CCNNNNNNNGG 3 cut(s) 554, 690, 691
AgsI TTSAA 7 cut(s) 99, 145, 446, 646, 745, 766, 841
AhdI GACNNNNNGTC 1 cut(s) 12
AhlI ACTAGT 1 cut(s) 286
AjnI CCWGG 1 cut(s) 481
AleI CACNNNNGTG 2 cut(s) 211, 773
AluBI AGCT 5 cut(s) 33, 281, 503, 673, 773
AluI AGCT 5 cut(s) 33, 281, 503, 673, 773
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 1 cut(s) 474
AoxI GGCC 2 cut(s) 150, 556
ApeKI GCWGC 1 cut(s) 500
AspLEI GCGC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 1 cut(s) 868
BalI TGGCCA 1 cut(s) 558
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 2 cut(s) 87, 688
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 512
BccI CCATC 4 cut(s) 908, 941, 995, 1004
BceAI ACGGC 1 cut(s) 1052
BciT130I CCWGG 1 cut(s) 483
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 33
BcuI ACTAGT 1 cut(s) 286
BfaI CTAG 4 cut(s) 117, 287, 674, 825
BisI GCNGC 2 cut(s) 261, 501
BlsI GCNGC 2 cut(s) 262, 502
Bme1390I CCNGG 2 cut(s) 154, 483
BmeRI GACNNNNNGTC 1 cut(s) 12
BmiI GGNNCC 2 cut(s) 480, 604
BmrFI CCNGG 2 cut(s) 154, 483
BmsI GCATC 2 cut(s) 174, 1032
BpiI GAAGAC 2 cut(s) 87, 688
BplI GAGNNNNNCTC 2 cut(s) 850, 882
BpmI CTGGAG 1 cut(s) 55
BpuEI CTTGAG 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 154
Bsa29I ATCGAT 1 cut(s) 1008
BsaAI YACGTR 1 cut(s) 350
BsaJI CCNNGG 4 cut(s) 9, 153, 474, 904
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 690, 691
BseBI CCWGG 1 cut(s) 483
BseCI ATCGAT 1 cut(s) 1008
BseDI CCNNGG 4 cut(s) 9, 153, 474, 904
BseGI GGATG 3 cut(s) 60, 900, 1023
BseLI CCNNNNNNNGG 3 cut(s) 554, 690, 691
BseMII CTCAG 2 cut(s) 322, 881
BseRI GAGGAG 2 cut(s) 285, 417
BseXI GCAGC 1 cut(s) 512
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 152, 558
BshVI ATCGAT 1 cut(s) 1008
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 3 cut(s) 554, 690, 691
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 863
BsnI GGCC 2 cut(s) 152, 558
Bsp1286I GDGCHC 2 cut(s) 23, 307
Bsp143I GATC 1 cut(s) 466
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 260, 548
BspANI GGCC 2 cut(s) 152, 558
BspCNI CTCAG 2 cut(s) 321, 880
BspDI ATCGAT 1 cut(s) 1008
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 2 cut(s) 480, 604
BspPI GGATC 1 cut(s) 474
BssECI CCNNGG 4 cut(s) 9, 153, 474, 904
BssMI GATC 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 474, 904
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 333, 990
BstDEI CTNAG 4 cut(s) 308, 867, 984, 1031
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 3 cut(s) 60, 900, 1023
BstHHI GCGC 1 cut(s) 386
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 1 cut(s) 466
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 257, 266, 332
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 2 cut(s) 425, 992
BstSCI CCNGG 2 cut(s) 152, 481
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 2 cut(s) 87, 688
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 1008
BsuRI GGCC 2 cut(s) 152, 558
BsuTUI ATCGAT 1 cut(s) 1008
BtgI CCRYGG 1 cut(s) 474
BtsCI GGATG 3 cut(s) 60, 900, 1023
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 333, 990
CciI TCATGA 1 cut(s) 615
CfoI GCGC 1 cut(s) 386
ClaI ATCGAT 1 cut(s) 1008
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 1 cut(s) 46
CviAII CATG 7 cut(s) 223, 422, 475, 616, 664, 835, 989
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 4 cut(s) 308, 867, 984, 1031
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DriI GACNNNNNGTC 1 cut(s) 12
EaeI YGGCCR 1 cut(s) 556
Eam1105I GACNNNNNGTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 474, 904
Eco24I GRGCYC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 1038
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 474, 904
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 2 cut(s) 474, 904
FaeI CATG 7 cut(s) 226, 425, 478, 619, 667, 838, 992
FaqI GGGAC 1 cut(s) 863
FatI CATG 7 cut(s) 222, 421, 474, 615, 663, 834, 988
FauI CCCGC 1 cut(s) 555
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 261, 501
FokI GGATG 3 cut(s) 67, 887, 1010
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 261, 501
FspBI CTAG 4 cut(s) 117, 287, 674, 825
GlaI GCGC 1 cut(s) 385
GluI GCNGC 2 cut(s) 261, 501
GsuI CTGGAG 1 cut(s) 55
HaeIII GGCC 2 cut(s) 152, 558
HapII CCGG 1 cut(s) 153
HhaI GCGC 1 cut(s) 386
Hin1II CATG 7 cut(s) 226, 425, 478, 619, 667, 838, 992
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HincII GTYRAC 1 cut(s) 651
HindII GTYRAC 1 cut(s) 651
HinfI GANTC 7 cut(s) 41, 82, 177, 446, 667, 859, 1005
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 4 cut(s) 577, 651, 679, 804
Hpy188I TCNGA 5 cut(s) 18, 64, 170, 182, 1018
Hpy188III TCNNGA 2 cut(s) 431, 616
Hpy8I GTNNAC 4 cut(s) 577, 651, 679, 804
HpyAV CCTTC 3 cut(s) 130, 928, 1013
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4IV ACGT 2 cut(s) 349, 436
HpyCH4V TGCA 4 cut(s) 275, 335, 500, 992
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 266, 332
HpyF3I CTNAG 4 cut(s) 308, 867, 984, 1031
HpySE526I ACGT 2 cut(s) 349, 436
Hsp92II CATG 7 cut(s) 226, 425, 478, 619, 667, 838, 992
HspAI GCGC 1 cut(s) 384
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 2 cut(s) 174, 1032
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 4 cut(s) 117, 287, 674, 825
MaeII ACGT 2 cut(s) 349, 436
MaeIII GTNAC 4 cut(s) 289, 451, 874, 895
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 7 cut(s) 87, 142, 145, 688, 911, 914, 1010
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 23, 307
MlsI TGGCCA 1 cut(s) 558
MluCI AATT 6 cut(s) 246, 253, 315, 636, 736, 1045
MluNI TGGCCA 1 cut(s) 558
MlyI GAGTC 3 cut(s) 35, 186, 868
MmeI TCCRAC 2 cut(s) 160, 887
MnlI CCTC 9 cut(s) 4, 70, 176, 306, 348, 438, 902, 979, 1038
Mox20I TGGCCA 1 cut(s) 558
MscI TGGCCA 1 cut(s) 558
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 4 cut(s) 211, 773, 881, 993
Msp20I TGGCCA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 2 cut(s) 154, 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 257, 266, 332
NciI CCSGG 1 cut(s) 154
NcoI CCATGG 1 cut(s) 474
NdeII GATC 1 cut(s) 466
NlaIII CATG 7 cut(s) 226, 425, 478, 619, 667, 838, 992
NlaIV GGNNCC 2 cut(s) 480, 604
NmuCI GTSAC 1 cut(s) 874
NspI RCATGY 2 cut(s) 425, 992
OliI CACNNNNGTG 2 cut(s) 211, 773
PaeI GCATGC 1 cut(s) 992
PagI TCATGA 1 cut(s) 615
PfeI GAWTC 4 cut(s) 82, 446, 667, 1005
PflFI GACNNNGTC 1 cut(s) 858
PkrI GCNGC 2 cut(s) 262, 502
PleI GAGTC 3 cut(s) 35, 185, 867
PpsI GAGTC 3 cut(s) 35, 185, 867
Ppu21I YACGTR 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 2 cut(s) 480, 604
PsuI RGATCY 1 cut(s) 466
PsyI GACNNNGTC 1 cut(s) 858
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 4 cut(s) 211, 773, 881, 993
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 2 cut(s) 261, 501
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 3 cut(s) 35, 186, 868
ScrFI CCNGG 2 cut(s) 154, 483
SduI GDGCHC 2 cut(s) 23, 307
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 2 cut(s) 174, 1032
SmiMI CAYNNNNRTG 4 cut(s) 211, 773, 881, 993
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SpeI ACTAGT 1 cut(s) 286
SphI GCATGC 1 cut(s) 992
Sse9I AATT 6 cut(s) 246, 253, 315, 636, 736, 1045
SsiI CCGC 2 cut(s) 260, 548
SspMI CTAG 4 cut(s) 117, 287, 674, 825
StyD4I CCNGG 2 cut(s) 152, 481
StyI CCWWGG 2 cut(s) 474, 904
TaaI ACNGT 1 cut(s) 77
TaiI ACGT 2 cut(s) 352, 439
TaqI TCGA 1 cut(s) 1008
TaqII GACCGA 2 cut(s) 23, 601
TasI AATT 6 cut(s) 246, 253, 315, 636, 736, 1045
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 4 cut(s) 82, 446, 667, 1005
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 5 cut(s) 112, 350, 680, 749, 1011
TspGWI ACGGA 1 cut(s) 961
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 1 cut(s) 858
XceI RCATGY 2 cut(s) 425, 992
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 4 cut(s) 117, 287, 674, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.