FvH4_5g30890

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014749
Physical Location & Seq
Forward (+)
21765942 .. 21770543
4602 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30890.t1

Sequence Viewer

Length: 582 bp
ATGATGAGCATGAAGAGCAGCACTAGTACTGCTGCTAAGAAGAAGAAGACGACGATGATGCCGAGGGGGTTCATGTGCCACTCTCAGGCCGCAACCGCGGTGTGCGTCAGCAGCGCCGATGCAGCTCGGTCAGATATCATTTCATCAGGTGCCAGAAATACGAGAATGATCAACAACAACTACAATGCCAGGTACTCAAAGATCGTCGAGTCACGTAGGTTTGTGAATCCAGCTGATCATGGAAACAAACCGGTCTTGTTGTCTTCCATGATCAAGAGAGAGCCGACTCTGAACTACCACCACCACCATCATTACTCGAAGCCGAAGATGTCACTGCAGAAAACACCTTCAGTTCTACCTTCTGCAGATAATGTCTTCCAGGTGGTTGTTATGAGGGTCGCTCTTCATTGCCAAGGATGTGCTGGTAAAGTGAAGAAACATCTTTCTAAAATGGAAGGGGTTACATCGTTCAGCATAGACCTAGAGACAAAGATGGTAACTGTAAGAGGACACGTGTCACCAGTTGGAGTTGTGGAGAGCATTTCCAAGGTTAAAAAGGCAGAGTTACTGCCCAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

194

Amino Acids

21.18

Weight (kDa)

10.13

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 131 - 185 1.7e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccII CGCG 1 cut(s) 98
AciI CCGC 3 cut(s) 90, 96, 98
AcuI CTGAAG 1 cut(s) 333
AcvI CACGTG 1 cut(s) 514
AfaI GTAC 2 cut(s) 28, 194
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 2 cut(s) 511, 513
AgeI ACCGGT 1 cut(s) 250
AhlI ACTAGT 1 cut(s) 23
AjnI CCWGG 2 cut(s) 188, 378
AluBI AGCT 2 cut(s) 125, 233
AluI AGCT 2 cut(s) 125, 233
Alw26I GTCTC 1 cut(s) 479
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 4 cut(s) 18, 32, 111, 122
AsiGI ACCGGT 1 cut(s) 250
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 1 cut(s) 510
BanI GGYRCC 1 cut(s) 149
BbrPI CACGTG 1 cut(s) 514
BbsI GAAGAC 3 cut(s) 53, 255, 367
BbvI GCAGC 4 cut(s) 19, 30, 123, 134
BccI CCATC 2 cut(s) 315, 487
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 2 cut(s) 190, 380
BclI TGATCA 3 cut(s) 168, 235, 270
BcoDI GTCTC 1 cut(s) 479
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 2 cut(s) 24, 482
BfmI CTRYAG 2 cut(s) 335, 363
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 5 cut(s) 19, 33, 90, 112, 123
BlsI GCNGC 5 cut(s) 20, 34, 91, 113, 124
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 2 cut(s) 190, 380
BmiI GGNNCC 1 cut(s) 151
BmrFI CCNGG 2 cut(s) 190, 380
BmsI GCATC 2 cut(s) 48, 109
BoxI GACNNNNGTC 1 cut(s) 514
BpiI GAAGAC 3 cut(s) 53, 255, 367
BplI GAGNNNNNCTC 2 cut(s) 385, 417
BsaAI YACGTR 2 cut(s) 215, 514
BsaJI CCNNGG 4 cut(s) 62, 96, 412, 546
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 521
Bse3DI GCAATG 1 cut(s) 406
BseBI CCWGG 2 cut(s) 190, 380
BseDI CCNNGG 4 cut(s) 62, 96, 412, 546
BseGI GGATG 1 cut(s) 422
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 1 cut(s) 521
BseXI GCAGC 4 cut(s) 19, 30, 123, 134
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 1 cut(s) 89
BshNI GGYRCC 1 cut(s) 149
BshTI ACCGGT 1 cut(s) 250
BsiSI CCGG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 479
BsnI GGCC 1 cut(s) 89
Bsp143I GATC 4 cut(s) 168, 201, 235, 270
BspACI CCGC 3 cut(s) 90, 96, 98
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 97
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 151
BspMAI CTGCAG 2 cut(s) 339, 367
BspQI GCTCTTC 2 cut(s) 8, 408
BspT107I GGYRCC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 1 cut(s) 521
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 4 cut(s) 62, 96, 412, 546
BssMI GATC 4 cut(s) 168, 201, 235, 270
BssT1I CCWWGG 2 cut(s) 412, 546
Bst2UI CCWGG 2 cut(s) 190, 380
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 2 cut(s) 8, 408
BstBAI YACGTR 2 cut(s) 215, 514
BstDEI CTNAG 2 cut(s) 36, 84
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 422
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 4 cut(s) 171, 204, 238, 273
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 4 cut(s) 168, 201, 235, 270
BstMWI GCNNNNNNNGC 4 cut(s) 15, 95, 111, 122
BstNI CCWGG 2 cut(s) 190, 380
BstPAI GACNNNNGTC 1 cut(s) 514
BstSCI CCNGG 2 cut(s) 188, 378
BstSFI CTRYAG 2 cut(s) 335, 363
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 4 cut(s) 19, 30, 123, 134
BstV2I GAAGAC 3 cut(s) 53, 255, 367
BsuRI GGCC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 422
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 250
Cfr42I CCGCGG 1 cut(s) 99
CseI GACGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 27, 193
CspAI ACCGGT 1 cut(s) 250
CviAII CATG 4 cut(s) 10, 73, 239, 268
CviJI RGCY 5 cut(s) 89, 125, 233, 283, 322
CviKI_1 RGCY 5 cut(s) 89, 125, 233, 283, 322
CviQI GTAC 2 cut(s) 27, 193
DdeI CTNAG 2 cut(s) 36, 84
DpnI GATC 4 cut(s) 170, 203, 237, 272
DpnII GATC 4 cut(s) 168, 201, 235, 270
Eam1104I CTCTTC 2 cut(s) 8, 408
EarI CTCTTC 2 cut(s) 8, 408
Eco130I CCWWGG 2 cut(s) 412, 546
Eco32I GATATC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 333
Eco72I CACGTG 1 cut(s) 514
EcoRII CCWGG 2 cut(s) 188, 378
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 412, 546
ErhI CCWWGG 2 cut(s) 412, 546
FaeI CATG 4 cut(s) 13, 76, 242, 271
FaiI YATR 6 cut(s) 11, 74, 240, 269, 392, 476
FatI CATG 4 cut(s) 9, 72, 238, 267
FbaI TGATCA 3 cut(s) 168, 235, 270
Fnu4HI GCNGC 5 cut(s) 19, 33, 90, 112, 123
FokI GGATG 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 19, 33, 90, 112, 123
FspBI CTAG 2 cut(s) 24, 482
GlaI GCGC 1 cut(s) 115
GluI GCNGC 5 cut(s) 19, 33, 90, 112, 123
GsaI CCCAGC 1 cut(s) 576
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 1 cut(s) 89
HapII CCGG 1 cut(s) 251
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 116
Hin1II CATG 4 cut(s) 13, 76, 242, 271
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 3 cut(s) 209, 226, 286
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 510
Hpy188I TCNGA 2 cut(s) 133, 291
Hpy188III TCNNGA 1 cut(s) 274
Hpy99I CGWCG 2 cut(s) 55, 209
HpyAV CCTTC 3 cut(s) 357, 369, 449
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 2 cut(s) 214, 513
HpyCH4V TGCA 3 cut(s) 122, 337, 365
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 95, 111, 122
HpyF3I CTNAG 2 cut(s) 36, 84
HpySE526I ACGT 2 cut(s) 214, 513
Hsp92II CATG 4 cut(s) 13, 76, 242, 271
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 3 cut(s) 168, 235, 270
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 4 cut(s) 168, 201, 235, 270
LguI GCTCTTC 2 cut(s) 8, 408
Lsp1109I GCAGC 4 cut(s) 19, 30, 123, 134
LweI GCATC 2 cut(s) 48, 109
MaeI CTAG 2 cut(s) 24, 482
MaeII ACGT 2 cut(s) 214, 513
MaeIII GTNAC 6 cut(s) 210, 330, 460, 496, 516, 564
MalI GATC 4 cut(s) 170, 203, 237, 272
MboI GATC 4 cut(s) 168, 201, 235, 270
MboII GAAGA 9 cut(s) 25, 52, 55, 58, 255, 337, 367, 395, 445
MlyI GAGTC 2 cut(s) 218, 280
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 3 cut(s) 57, 387, 500
MseI TTAA 1 cut(s) 552
MspA1I CMGCKG 2 cut(s) 98, 233
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 2 cut(s) 190, 380
MvaI CCWGG 2 cut(s) 190, 380
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 4 cut(s) 15, 95, 111, 122
NdeII GATC 4 cut(s) 168, 201, 235, 270
NlaIII CATG 4 cut(s) 13, 76, 242, 271
NlaIV GGNNCC 1 cut(s) 151
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 3 cut(s) 210, 330, 516
PciSI GCTCTTC 2 cut(s) 8, 408
PcsI WCGNNNNNNNCGW 1 cut(s) 59
PfeI GAWTC 1 cut(s) 226
PinAI ACCGGT 1 cut(s) 250
PkrI GCNGC 5 cut(s) 20, 34, 91, 113, 124
PleI GAGTC 2 cut(s) 217, 280
PmaCI CACGTG 1 cut(s) 514
PmlI CACGTG 1 cut(s) 514
PpsI GAGTC 2 cut(s) 217, 280
Ppu21I YACGTR 2 cut(s) 215, 514
PshAI GACNNNNGTC 1 cut(s) 514
Psp6I CCWGG 2 cut(s) 188, 378
PspCI CACGTG 1 cut(s) 514
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 2 cut(s) 188, 378
PspN4I GGNNCC 1 cut(s) 151
PstI CTGCAG 2 cut(s) 339, 367
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 28, 194
RsaNI GTAC 2 cut(s) 27, 193
SacII CCGCGG 1 cut(s) 99
SapI GCTCTTC 2 cut(s) 8, 408
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 5 cut(s) 19, 33, 90, 112, 123
Sau3AI GATC 4 cut(s) 168, 201, 235, 270
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 2 cut(s) 218, 280
ScrFI CCNGG 2 cut(s) 190, 380
SfaNI GCATC 2 cut(s) 48, 109
SfcI CTRYAG 2 cut(s) 335, 363
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SpeI ACTAGT 1 cut(s) 23
SsiI CCGC 3 cut(s) 90, 96, 98
SspMI CTAG 2 cut(s) 24, 482
StyD4I CCNGG 2 cut(s) 188, 378
StyI CCWWGG 2 cut(s) 412, 546
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 2 cut(s) 217, 516
TaqI TCGA 2 cut(s) 207, 317
TaqII GACCGA 1 cut(s) 117
TatI WGTACW 1 cut(s) 26
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 3 cut(s) 210, 330, 516
TseI GCWGC 4 cut(s) 18, 32, 111, 122
Tsp45I GTSAC 3 cut(s) 210, 330, 516
TspDTI ATGAA 4 cut(s) 26, 61, 132, 395
TspRI CASTG 1 cut(s) 339
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XspI CTAG 2 cut(s) 24, 482
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.