Rh7AG382200

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014749
Physical Location & Seq
Forward (+)
49911005 .. 49913825
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG382200.1

Sequence Viewer

Length: 564 bp
ATGATGAACATGAAGAGCAGCACTGCTACTAAGAAGAAGATGACGATGATGCCGAGGGGGTTCATGTGCCACTCTCAGACAGCAACTGCCGTCTGCGTGAGCAGCGCCGATGCAGCTCGGTCCGCGATCGTCCCAGCTGCTAGAAATACGAGAATGATCAACAACTACAATGCCAGGTACTCGAAGATCGTTGAGTCACGTAGGTCTGGCAATTTAGCTGATCATGGAAAGAAACCAGTCTTGTTGTCTTCCCTGATCAAGAGAGAGTCGGCTTTGAACTACCACCACCATCATCACTCGAAGCCAAAGATGTCACTGCAGAAAACAGCGTCAGTTCTACCTATTACAGATAATGTCTTCCAGGTGGTTGTTATGAGGGTTGCTCTTCATTGTCAAGGATGTGCTGGAAAAGTGAAGAAACATCTTTCTAAAATGGAAGGGGTTACATCTTTCAGTATAGACCTGGAGACAAAGATGGTAACTGTAAGAGGACACGTGTCGCCAGTCAGAGTCGTGGAGAGCATTTCCAAGGTTAAAAAGGCAGAGTTACTGCCCTGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

187

Amino Acids

20.6

Weight (kDa)

10.25

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 125 - 179 3.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 123
AcvI CACGTG 1 cut(s) 496
AfaI GTAC 1 cut(s) 179
AflIII ACRYGT 2 cut(s) 493, 495
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 3 cut(s) 173, 360, 462
AluBI AGCT 3 cut(s) 116, 137, 218
AluI AGCT 3 cut(s) 116, 137, 218
Alw26I GTCTC 1 cut(s) 461
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 4 cut(s) 18, 102, 113, 137
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 120
AvaII GGWCC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 496
BbsI GAAGAC 2 cut(s) 240, 349
BbvI GCAGC 4 cut(s) 30, 114, 124, 125
BccI CCATC 2 cut(s) 297, 469
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 175, 362, 464
BclI TGATCA 3 cut(s) 156, 220, 255
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 2 cut(s) 317, 556
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 4 cut(s) 19, 103, 114, 138
BlsI GCNGC 4 cut(s) 20, 104, 115, 139
Bme1390I CCNGG 3 cut(s) 175, 362, 464
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 175, 362, 464
BmsI GCATC 2 cut(s) 39, 100
BoxI GACNNNNGTC 1 cut(s) 496
BpiI GAAGAC 2 cut(s) 240, 349
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BpmI CTGGAG 1 cut(s) 485
BsaAI YACGTR 2 cut(s) 200, 496
BsaJI CCNNGG 2 cut(s) 53, 528
Bse1I ACTGG 2 cut(s) 236, 503
BseBI CCWGG 3 cut(s) 175, 362, 464
BseDI CCNNGG 2 cut(s) 53, 528
BseGI GGATG 1 cut(s) 404
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 236, 503
BseXI GCAGC 4 cut(s) 30, 114, 124, 125
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 116
Bsp143I GATC 5 cut(s) 126, 156, 186, 220, 255
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 125
BspMAI CTGCAG 2 cut(s) 321, 560
BspQI GCTCTTC 2 cut(s) 8, 390
BsrI ACTGG 2 cut(s) 236, 503
BssECI CCNNGG 2 cut(s) 53, 528
BssMI GATC 5 cut(s) 126, 156, 186, 220, 255
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 3 cut(s) 175, 362, 464
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 2 cut(s) 8, 390
BstBAI YACGTR 2 cut(s) 200, 496
BstDEI CTNAG 2 cut(s) 30, 75
BstF5I GGATG 1 cut(s) 404
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 5 cut(s) 129, 159, 189, 223, 258
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 5 cut(s) 126, 156, 186, 220, 255
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 102, 113, 122
BstNI CCWGG 3 cut(s) 175, 362, 464
BstPAI GACNNNNGTC 1 cut(s) 496
BstSCI CCNGG 3 cut(s) 173, 360, 462
BstSFI CTRYAG 2 cut(s) 317, 556
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 4 cut(s) 30, 114, 124, 125
BstV2I GAAGAC 2 cut(s) 240, 349
BtsCI GGATG 1 cut(s) 404
BtsI GCAGTG 2 cut(s) 21, 314
BtsIMutI CAGTG 2 cut(s) 21, 314
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 1 cut(s) 318
Csp6I GTAC 1 cut(s) 178
CspI CGGWCCG 1 cut(s) 120
CviAII CATG 3 cut(s) 10, 64, 224
CviJI RGCY 5 cut(s) 116, 137, 218, 272, 304
CviKI_1 RGCY 5 cut(s) 116, 137, 218, 272, 304
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 30, 75
DpnI GATC 5 cut(s) 128, 158, 188, 222, 257
DpnII GATC 5 cut(s) 126, 156, 186, 220, 255
Eam1104I CTCTTC 2 cut(s) 8, 390
EarI CTCTTC 2 cut(s) 8, 390
Eco130I CCWWGG 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 120
Eco72I CACGTG 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 173, 360, 462
EcoT14I CCWWGG 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 3 cut(s) 13, 67, 227
FaiI YATR 5 cut(s) 11, 65, 225, 374, 458
FaqI GGGAC 1 cut(s) 116
FatI CATG 3 cut(s) 9, 63, 223
FbaI TGATCA 3 cut(s) 156, 220, 255
Fnu4HI GCNGC 4 cut(s) 19, 103, 114, 138
FokI GGATG 1 cut(s) 411
Fsp4HI GCNGC 4 cut(s) 19, 103, 114, 138
FspBI CTAG 1 cut(s) 141
GlaI GCGC 1 cut(s) 106
GluI GCNGC 4 cut(s) 19, 103, 114, 138
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 108
HgaI GACGC 1 cut(s) 318
HhaI GCGC 1 cut(s) 107
Hin1II CATG 3 cut(s) 13, 67, 227
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 194, 266, 510
Hpy188I TCNGA 2 cut(s) 78, 509
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 2 cut(s) 199, 495
HpyCH4V TGCA 3 cut(s) 113, 319, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 113, 122
HpyF3I CTNAG 2 cut(s) 30, 75
HpySE526I ACGT 2 cut(s) 199, 495
Hsp92II CATG 3 cut(s) 13, 67, 227
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 3 cut(s) 156, 220, 255
Kzo9I GATC 5 cut(s) 126, 156, 186, 220, 255
LguI GCTCTTC 2 cut(s) 8, 390
Lsp1109I GCAGC 4 cut(s) 30, 114, 124, 125
LweI GCATC 2 cut(s) 39, 100
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 199, 495
MaeIII GTNAC 5 cut(s) 195, 312, 442, 478, 546
MalI GATC 5 cut(s) 128, 158, 188, 222, 257
MboI GATC 5 cut(s) 126, 156, 186, 220, 255
MboII GAAGA 8 cut(s) 25, 46, 49, 196, 240, 349, 377, 427
MluCI AATT 1 cut(s) 211
MlyI GAGTC 3 cut(s) 203, 275, 519
MnlI CCTC 3 cut(s) 48, 369, 482
MseI TTAA 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 175, 362, 464
MvaI CCWGG 3 cut(s) 175, 362, 464
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 102, 113, 122
NdeII GATC 5 cut(s) 126, 156, 186, 220, 255
NlaIII CATG 3 cut(s) 13, 67, 227
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 2 cut(s) 195, 312
PciSI GCTCTTC 2 cut(s) 8, 390
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PkrI GCNGC 4 cut(s) 20, 104, 115, 139
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 3 cut(s) 202, 274, 518
PmaCI CACGTG 1 cut(s) 496
PmlI CACGTG 1 cut(s) 496
PpsI GAGTC 3 cut(s) 202, 274, 518
Ppu21I YACGTR 2 cut(s) 200, 496
PshAI GACNNNNGTC 1 cut(s) 496
Psp6I CCWGG 3 cut(s) 173, 360, 462
PspCI CACGTG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 173, 360, 462
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 2 cut(s) 321, 560
PstNI CAGNNNCTG 1 cut(s) 86
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SapI GCTCTTC 2 cut(s) 8, 390
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 4 cut(s) 19, 103, 114, 138
Sau3AI GATC 5 cut(s) 126, 156, 186, 220, 255
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 3 cut(s) 203, 275, 519
ScrFI CCNGG 3 cut(s) 175, 362, 464
SfaNI GCATC 2 cut(s) 39, 100
SfcI CTRYAG 2 cut(s) 317, 556
SinI GGWCC 1 cut(s) 120
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 173, 360, 462
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 2 cut(s) 202, 498
TaqI TCGA 2 cut(s) 182, 299
TaqII GACCGA 1 cut(s) 108
TasI AATT 1 cut(s) 211
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 2 cut(s) 28, 321
TseFI GTSAC 2 cut(s) 195, 312
TseI GCWGC 4 cut(s) 18, 102, 113, 137
Tsp45I GTSAC 2 cut(s) 195, 312
TspDTI ATGAA 4 cut(s) 20, 26, 52, 377
TspRI CASTG 2 cut(s) 28, 321
VpaK11BI GGWCC 1 cut(s) 120
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.