MD15G1412000.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014749
Physical Location & Seq
Reverse (-)
51156707 .. 51158850
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1412000.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAACATGAAAAGCCGCAGCGGTACCAAGGAGAAGATGAAGAAGAAGATGATGATGAGAGGGTTCATGTGCCAGTCTCAGGTAGCAACTGCGGTGTGTATGACTGATGCTCGTTCCATTATCATGCCGCCAACAAGACCAGCTGCAGCTACCACTGTCCGCCACAATCACAATGCTGGACATGGGCATTACATGATGATGAGAAGATCAACTAGTACTAATAACGCTGGTGATGGCGGCAGCGGTGGTCAATACTACTACCCCAGGCTCCTCAACACTCCTCCAGCTCATGGCCATGAGACATCCTACTCTCACGGTCGCAGGCGAAGCACTACCACTAGCAGAATTCTTAACCACCATGATCAGCAGCCAATGTCAAAGTCGATCCGAAAACTACCATTATCTGATCCAGATTTACTACCACCGTCATCATCCGCGGACAATGTCTTCCAGGTGGTTGTTATGAGAGTTTCCATTCATTGCCAAGGATGTGCTGGTAAACTGAAGAAACATCTCTCTAAGATGGAAGGGGTTACATCTTTCAGTATTGACCTAGAGACAAAGATGGTAACTGTTAGGGGACACGTTTCAGCAGTTGGAGTCGTGGAGAGCATTTCAAAAGTGAAGAAGGCAGAGTTATTAGCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

217

Amino Acids

23.72

Weight (kDa)

10.21

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 155 - 209 8.4e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 290
AccII CGCG 1 cut(s) 437
AciI CCGC 9 cut(s) 16, 21, 92, 128, 160, 237, 243, 435, 437
AclWI GGATC 2 cut(s) 379, 401
AcoI YGGCCR 1 cut(s) 292
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 2 cut(s) 25, 217
AfiI CCNNNNNNNGG 2 cut(s) 79, 290
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 1 cut(s) 618
AhlI ACTAGT 1 cut(s) 212
AjnI CCWGG 2 cut(s) 263, 450
AluBI AGCT 3 cut(s) 143, 149, 287
AluI AGCT 3 cut(s) 143, 149, 287
Alw26I GTCTC 3 cut(s) 81, 293, 551
AlwI GGATC 2 cut(s) 379, 401
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 5 cut(s) 18, 143, 146, 240, 367
ApoI RAATTY 1 cut(s) 345
Asp718I GGTACC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 242
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 439
BbvI GCAGC 5 cut(s) 30, 130, 158, 252, 379
BccI CCATC 3 cut(s) 227, 517, 559
BciT130I CCWGG 2 cut(s) 265, 452
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 81, 293, 551
BcuI ACTAGT 1 cut(s) 212
BfaI CTAG 3 cut(s) 213, 339, 554
BfmI CTRYAG 1 cut(s) 144
BisI GCNGC 8 cut(s) 16, 19, 128, 144, 147, 238, 241, 368
BlsI GCNGC 8 cut(s) 17, 20, 129, 145, 148, 239, 242, 369
BmcAI AGTACT 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 265, 452
BmiI GGNNCC 2 cut(s) 25, 269
BmrFI CCNGG 2 cut(s) 265, 452
BmsI GCATC 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 439
BpmI CTGGAG 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 27, 263, 435, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 290
Bse1I ACTGG 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 2 cut(s) 265, 452
BseDI CCNNGG 4 cut(s) 27, 263, 435, 484
BseGI GGATG 3 cut(s) 302, 431, 494
BseLI CCNNNNNNNGG 2 cut(s) 79, 290
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 2 cut(s) 260, 270
BseXI GCAGC 5 cut(s) 30, 130, 158, 252, 379
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 2 cut(s) 79, 290
BsmAI GTCTC 3 cut(s) 81, 293, 551
BsmFI GGGAC 1 cut(s) 594
BsnI GGCC 1 cut(s) 294
Bsp143I GATC 4 cut(s) 206, 361, 384, 406
BspACI CCGC 9 cut(s) 16, 21, 92, 128, 160, 237, 243, 435, 437
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 91
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 2 cut(s) 25, 269
BspMAI CTGCAG 1 cut(s) 148
BspPI GGATC 2 cut(s) 379, 401
BspT107I GGYRCC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 478
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 27, 263, 435, 484
BssMI GATC 4 cut(s) 206, 361, 384, 406
BssT1I CCWWGG 2 cut(s) 27, 484
Bst2UI CCWGG 2 cut(s) 265, 452
Bst4CI ACNGT 4 cut(s) 157, 317, 426, 574
BstC8I GCNNGC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 78, 519
BstDSI CCRYGG 1 cut(s) 435
BstF5I GGATG 3 cut(s) 302, 431, 494
BstFNI CGCG 1 cut(s) 437
BstKTI GATC 4 cut(s) 209, 364, 387, 409
BstMAI GTCTC 3 cut(s) 81, 293, 551
BstMBI GATC 4 cut(s) 206, 361, 384, 406
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 1 cut(s) 327
BstNI CCWGG 2 cut(s) 265, 452
BstSCI CCNGG 2 cut(s) 263, 450
BstSFI CTRYAG 1 cut(s) 144
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 5 cut(s) 30, 130, 158, 252, 379
BstV2I GAAGAC 1 cut(s) 439
BsuRI GGCC 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 435
BtsCI GGATG 3 cut(s) 302, 431, 494
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 323
Cfr42I CCGCGG 1 cut(s) 438
Csp6I GTAC 2 cut(s) 24, 216
CviAII CATG 8 cut(s) 7, 67, 124, 182, 193, 290, 296, 359
CviJI RGCY 8 cut(s) 15, 143, 149, 268, 287, 294, 370, 644
CviKI_1 RGCY 8 cut(s) 15, 143, 149, 268, 287, 294, 370, 644
CviQI GTAC 2 cut(s) 24, 216
DdeI CTNAG 2 cut(s) 78, 519
DpnI GATC 4 cut(s) 208, 363, 386, 408
DpnII GATC 4 cut(s) 206, 361, 384, 406
EaeI YGGCCR 1 cut(s) 292
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 27, 484
Eco57I CTGAAG 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 2 cut(s) 263, 450
EcoT14I CCWWGG 2 cut(s) 27, 484
ErhI CCWWGG 2 cut(s) 27, 484
FaeI CATG 8 cut(s) 10, 70, 127, 185, 196, 293, 299, 362
FaqI GGGAC 1 cut(s) 594
FatI CATG 8 cut(s) 6, 66, 123, 181, 192, 289, 295, 358
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 8 cut(s) 16, 19, 128, 144, 147, 238, 241, 368
FokI GGATG 3 cut(s) 289, 418, 501
Fsp4HI GCNGC 8 cut(s) 16, 19, 128, 144, 147, 238, 241, 368
FspBI CTAG 3 cut(s) 213, 339, 554
GluI GCNGC 8 cut(s) 16, 19, 128, 144, 147, 238, 241, 368
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 1 cut(s) 294
Hin1II CATG 8 cut(s) 10, 70, 127, 185, 196, 293, 299, 362
HinfI GANTC 1 cut(s) 600
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 500
Hpy188I TCNGA 2 cut(s) 389, 406
Hpy188III TCNNGA 2 cut(s) 410, 648
Hpy8I GTNNAC 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 521, 622
HpyCH4III ACNGT 4 cut(s) 157, 317, 426, 574
HpyCH4IV ACGT 1 cut(s) 585
HpyCH4V TGCA 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 1 cut(s) 327
HpyF3I CTNAG 2 cut(s) 78, 519
HpySE526I ACGT 1 cut(s) 585
Hsp92II CATG 8 cut(s) 10, 70, 127, 185, 196, 293, 299, 362
KpnI GGTACC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 438
Kzo9I GATC 4 cut(s) 206, 361, 384, 406
LmnI GCTCC 1 cut(s) 273
Lsp1109I GCAGC 5 cut(s) 30, 130, 158, 252, 379
LweI GCATC 1 cut(s) 97
MaeI CTAG 3 cut(s) 213, 339, 554
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 2 cut(s) 532, 568
MalI GATC 4 cut(s) 208, 363, 386, 408
MboI GATC 4 cut(s) 206, 361, 384, 406
MboII GAAGA 8 cut(s) 46, 52, 55, 58, 216, 439, 517, 637
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 1 cut(s) 345
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 1 cut(s) 609
MmeI TCCRAC 1 cut(s) 577
MnlI CCTC 3 cut(s) 53, 281, 291
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 3 cut(s) 122, 197, 294
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 4 cut(s) 21, 143, 243, 437
MspR9I CCNGG 2 cut(s) 265, 452
MvaI CCWGG 2 cut(s) 265, 452
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 1 cut(s) 327
NdeII GATC 4 cut(s) 206, 361, 384, 406
NlaIII CATG 8 cut(s) 10, 70, 127, 185, 196, 293, 299, 362
NlaIV GGNNCC 2 cut(s) 25, 269
PflFI GACNNNGTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 8 cut(s) 17, 20, 129, 145, 148, 239, 242, 369
PleI GAGTC 1 cut(s) 608
PpsI GAGTC 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 263, 450
PspGI CCWGG 2 cut(s) 263, 450
PspN4I GGNNCC 2 cut(s) 25, 269
PstI CTGCAG 1 cut(s) 148
PsyI GACNNNGTC 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 2 cut(s) 25, 217
RsaNI GTAC 2 cut(s) 24, 216
RseI CAYNNNNRTG 3 cut(s) 122, 197, 294
SacII CCGCGG 1 cut(s) 438
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 8 cut(s) 16, 19, 128, 144, 147, 238, 241, 368
Sau3AI GATC 4 cut(s) 206, 361, 384, 406
ScaI AGTACT 1 cut(s) 217
SchI GAGTC 1 cut(s) 609
ScrFI CCNGG 2 cut(s) 265, 452
SetI ASST 7 cut(s) 84, 145, 151, 289, 456, 555, 588
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 144
Sfr303I CCGCGG 1 cut(s) 438
SgrBI CCGCGG 1 cut(s) 438
SmiMI CAYNNNNRTG 3 cut(s) 122, 197, 294
SpeI ACTAGT 1 cut(s) 212
Sse9I AATT 1 cut(s) 345
SsiI CCGC 9 cut(s) 16, 21, 92, 128, 160, 237, 243, 435, 437
SspMI CTAG 3 cut(s) 213, 339, 554
StyD4I CCNGG 2 cut(s) 263, 450
StyI CCWWGG 2 cut(s) 27, 484
TaaI ACNGT 4 cut(s) 157, 317, 426, 574
TaiI ACGT 1 cut(s) 588
TaqI TCGA 1 cut(s) 383
TasI AATT 1 cut(s) 345
TatI WGTACW 1 cut(s) 215
TauI GCSGC 3 cut(s) 18, 130, 240
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 160
TseI GCWGC 5 cut(s) 18, 143, 146, 240, 367
TspDTI ATGAA 5 cut(s) 17, 23, 53, 55, 467
TspRI CASTG 1 cut(s) 160
Tth111I GACNNNGTC 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 290
XapI RAATTY 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
XspI CTAG 3 cut(s) 213, 339, 554
ZrmI AGTACT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.