Rorug07G0235200

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014749
Physical Location & Seq
Forward (+)
21603349 .. 21604841
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0235200.1

Sequence Viewer

Length: 333 bp
ATGTATTTTGAGGAAGAGGAGGAGGCCTTTGAGCACACACTCCTAGTGGTCCATGAGGTCTCGGTCTATAAAATTCCACCACGGTCAACGTCGGGTGGTTACAGTGAGTGGCTCCAGTCTGACAGGATATGGTCAGGAAGATTGCGCGTTGTGTCGTGCAAGGAACGGTGTGAGATCCGATTGGAGGATCCAAGCTCCAGCCAGCGCGAGACCTCAGTGGAGATGGTGCTTGATATTCACCCAGCTGTGAATCATAGATTGAAGAGGAAACATCCTTCAACAAATGGATCCGGAATAACAAAGACAATTGCAGCTGGATGGGTGGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

110

Amino Acids

12.6

Weight (kDa)

6.2

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 10 - 68 9e-15 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 147, 207
AccIII TCCGGA 1 cut(s) 290
AclWI GGATC 5 cut(s) 169, 182, 195, 282, 295
AcsI RAATTY 1 cut(s) 72
AfiI CCNNNNNNNGG 1 cut(s) 184
AgsI TTSAA 2 cut(s) 262, 279
AluBI AGCT 3 cut(s) 195, 245, 314
AluI AGCT 3 cut(s) 195, 245, 314
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 64, 203
AlwI GGATC 5 cut(s) 169, 182, 195, 282, 295
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 72
AspLEI GCGC 2 cut(s) 147, 207
AspS9I GGNCC 1 cut(s) 49
AsuHPI GGTGA 1 cut(s) 230
AvaII GGWCC 1 cut(s) 49
BamHI GGATCC 2 cut(s) 187, 287
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 1 cut(s) 323
BccI CCATC 2 cut(s) 217, 312
BcoDI GTCTC 2 cut(s) 64, 203
BfaI CTAG 1 cut(s) 44
BisI GCNGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 113, 189, 289
BpmI CTGGAG 2 cut(s) 98, 181
BsaI GGTCTC 2 cut(s) 64, 203
BsaJI CCNNGG 1 cut(s) 80
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 115
BseAI TCCGGA 1 cut(s) 290
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 2 cut(s) 271, 323
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 2 cut(s) 32, 35
BseXI GCAGC 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 241
Bsh1236I CGCG 2 cut(s) 147, 207
BshFI GGCC 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 2 cut(s) 64, 203
BsnI GGCC 1 cut(s) 26
Bso31I GGTCTC 2 cut(s) 64, 203
Bsp1286I GDGCHC 1 cut(s) 36
Bsp13I TCCGGA 1 cut(s) 290
Bsp143I GATC 3 cut(s) 174, 187, 287
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 1 cut(s) 227
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 2 cut(s) 147, 207
BspLI GGNNCC 3 cut(s) 113, 189, 289
BspPI GGATC 5 cut(s) 169, 182, 195, 282, 295
BspTNI GGTCTC 2 cut(s) 64, 203
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 3 cut(s) 174, 187, 287
Bst4CI ACNGT 3 cut(s) 84, 104, 168
Bst6I CTCTTC 2 cut(s) 9, 257
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 1 cut(s) 214
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 271, 323
BstFNI CGCG 2 cut(s) 147, 207
BstHHI GCGC 2 cut(s) 147, 207
BstKTI GATC 3 cut(s) 177, 190, 290
BstMAI GTCTC 2 cut(s) 64, 203
BstMBI GATC 3 cut(s) 174, 187, 287
BstUI CGCG 2 cut(s) 147, 207
BstV1I GCAGC 1 cut(s) 323
BstX2I RGATCY 3 cut(s) 174, 187, 287
BstYI RGATCY 3 cut(s) 174, 187, 287
BsuRI GGCC 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 2 cut(s) 271, 323
BtsIMutI CAGTG 2 cut(s) 109, 222
Cac8I GCNNGC 1 cut(s) 203
CfoI GCGC 2 cut(s) 147, 207
Cfr13I GGNCC 1 cut(s) 49
CviAII CATG 1 cut(s) 53
CviJI RGCY 7 cut(s) 26, 112, 195, 201, 245, 314, 326
CviKI_1 RGCY 7 cut(s) 26, 112, 195, 201, 245, 314, 326
DdeI CTNAG 1 cut(s) 214
DpnI GATC 3 cut(s) 176, 189, 289
DpnII GATC 3 cut(s) 174, 187, 287
Eam1104I CTCTTC 2 cut(s) 9, 257
EarI CTCTTC 2 cut(s) 9, 257
Eco147I AGGCCT 1 cut(s) 26
Eco31I GGTCTC 2 cut(s) 64, 203
Eco47I GGWCC 1 cut(s) 49
FaeI CATG 1 cut(s) 56
FaiI YATR 4 cut(s) 54, 69, 130, 255
FatI CATG 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 146, 206
GluI GCNGC 1 cut(s) 312
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 2 cut(s) 98, 181
HaeIII GGCC 1 cut(s) 26
HapII CCGG 1 cut(s) 291
HhaI GCGC 2 cut(s) 147, 207
Hin1II CATG 1 cut(s) 56
Hin6I GCGC 2 cut(s) 145, 205
HinP1I GCGC 2 cut(s) 145, 205
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 1 cut(s) 250
HpaII CCGG 1 cut(s) 291
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 3 cut(s) 121, 179, 332
Hpy188III TCNNGA 2 cut(s) 135, 291
Hpy8I GTNNAC 1 cut(s) 87
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 285
HpyCH4III ACNGT 3 cut(s) 84, 104, 168
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 2 cut(s) 159, 311
HpyF3I CTNAG 1 cut(s) 214
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 1 cut(s) 56
HspAI GCGC 2 cut(s) 145, 205
Kpn2I TCCGGA 1 cut(s) 290
Kzo9I GATC 3 cut(s) 174, 187, 287
LmnI GCTCC 2 cut(s) 117, 200
LpnPI CCDG 8 cut(s) 109, 120, 128, 211, 215, 255, 300, 304
Lsp1109I GCAGC 1 cut(s) 323
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 98
MalI GATC 3 cut(s) 176, 189, 289
MboI GATC 3 cut(s) 174, 187, 287
MboII GAAGA 3 cut(s) 26, 150, 274
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 3 cut(s) 174, 187, 287
MhlI GDGCHC 1 cut(s) 36
MluCI AATT 2 cut(s) 72, 306
MnlI CCTC 8 cut(s) 4, 10, 13, 16, 49, 178, 223, 258
MroI TCCGGA 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 245, 314
MspI CCGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 306
MvnI CGCG 2 cut(s) 147, 207
NdeII GATC 3 cut(s) 174, 187, 287
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 3 cut(s) 113, 189, 289
PceI AGGCCT 1 cut(s) 26
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 241
PspN4I GGNNCC 3 cut(s) 113, 189, 289
PspPI GGNCC 1 cut(s) 49
PsuI RGATCY 3 cut(s) 174, 187, 287
PvuII CAGCTG 2 cut(s) 245, 314
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 3 cut(s) 174, 187, 287
Sau96I GGNCC 1 cut(s) 49
SduI GDGCHC 1 cut(s) 36
SetI ASST 6 cut(s) 60, 92, 197, 215, 247, 316
SinI GGWCC 1 cut(s) 49
Sse9I AATT 2 cut(s) 72, 306
SseBI AGGCCT 1 cut(s) 26
SspMI CTAG 1 cut(s) 44
StuI AGGCCT 1 cut(s) 26
TaaI ACNGT 3 cut(s) 84, 104, 168
TaiI ACGT 1 cut(s) 92
TaqII GACCGA 1 cut(s) 52
TasI AATT 2 cut(s) 72, 306
TfiI GAWTC 1 cut(s) 250
TscAI CASTG 2 cut(s) 109, 222
TseI GCWGC 1 cut(s) 311
TspRI CASTG 2 cut(s) 109, 222
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 72
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.