Prupe.1G558400_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014749
Physical Location & Seq
Reverse (-)
45579639 .. 45581645
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G558400.2

Sequence Viewer

Length: 666 bp
ATGAACATAAAAAACAGTTCTACTAGTACTAAGGAGAAGAAGGATAAGAAGATGATGATCATGAGGGGCTTCATGTGCCAGTCTCCGGCAGCGACCGCCGTTTGTATGAGTAGAGCCGATGCTCGCTCAGTTATGGTGCCTAGAAGATCATCCACTAGTAGTCTTAATAATAATTTTGATATTAATTTCAATCGCACACACTACACAAGATCACTGCTCAACAGCAACAAATACTACTACTCCAGGCTCCTCACTTCTCCTCCTCCTCCTCATCATGTTCATGATCATGGCAAAATATCGCCCTTATTAATTATGCCGTCCACCAGCAAACAAGTAGGAGCTGATCATCATCATGATCTTCTTCCTCTTACCCATCAGCAGCCAATGTCCTCAAAGACGATCGAAAAAAGACCGTCCTCTCATTCAGATTCAGTCCGGCCTTCATCCGCCGACGACAATGTATTCCAGGTGGTTGTTATGAGAGTTTCTATTCACTGTCAAGGATGTGCTGGTAAAGTGAAGAAACATCTTTCTAAAATGGAAGGAGTTACATCATTCAGTATCGACCTAGAGACAAAGATGGTAACAGTTAGAGGACACGTGTCACCATCTGCAGTGATGGAAAGCATTTCAAAAGTGAAAAAAGCAGAGTTATTACCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

222

Amino Acids

24.58

Weight (kDa)

9.91

Isoelectric Point (pI)

63.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 136
AciI CCGC 2 cut(s) 96, 447
AcvI CACGTG 1 cut(s) 601
AfaI GTAC 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 2 cut(s) 598, 600
AgsI TTSAA 2 cut(s) 190, 633
AhlI ACTAGT 2 cut(s) 23, 155
AjnI CCWGG 2 cut(s) 242, 465
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
Alw26I GTCTC 2 cut(s) 87, 566
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 89, 379
AseI ATTAAT 2 cut(s) 183, 308
AsuHPI GGTGA 1 cut(s) 597
BanI GGYRCC 1 cut(s) 136
BbrPI CACGTG 1 cut(s) 601
BbvI GCAGC 2 cut(s) 101, 391
BccI CCATC 4 cut(s) 381, 574, 613, 616
BceAI ACGGC 2 cut(s) 83, 301
BciT130I CCWGG 2 cut(s) 244, 467
BclI TGATCA 3 cut(s) 57, 283, 343
BcoDI GTCTC 2 cut(s) 87, 566
BcuI ACTAGT 2 cut(s) 23, 155
BfaI CTAG 4 cut(s) 24, 141, 156, 569
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 2 cut(s) 90, 380
BlsI GCNGC 2 cut(s) 91, 381
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 2 cut(s) 244, 467
BmiI GGNNCC 2 cut(s) 138, 248
BmrFI CCNGG 2 cut(s) 244, 467
BmsI GCATC 1 cut(s) 109
BoxI GACNNNNGTC 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 601
BsaBI GATNNNNATC 2 cut(s) 56, 348
BsaXI ACNNNNNCTCC 4 cut(s) 224, 244, 254, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 79
Bse8I GATNNNNATC 2 cut(s) 56, 348
BseBI CCWGG 2 cut(s) 244, 467
BseGI GGATG 3 cut(s) 149, 443, 509
BseJI GATNNNNATC 2 cut(s) 56, 348
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 5 cut(s) 239, 249, 252, 255, 258
BseXI GCAGC 2 cut(s) 101, 391
Bsh1285I CGRYCG 2 cut(s) 96, 402
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 136
BsiEI CGRYCG 2 cut(s) 96, 402
BsiSI CCGG 2 cut(s) 86, 436
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 2 cut(s) 87, 566
BsnI GGCC 1 cut(s) 439
Bsp143I GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
BspACI CCGC 2 cut(s) 96, 447
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 140
BspHI TCATGA 3 cut(s) 60, 280, 352
BspLI GGNNCC 2 cut(s) 138, 248
BspMAI CTGCAG 1 cut(s) 616
BspT107I GGYRCC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 79
BssMI GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
Bst2UI CCWGG 2 cut(s) 244, 467
Bst4CI ACNGT 4 cut(s) 17, 414, 497, 589
BstBAI YACGTR 1 cut(s) 601
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 2 cut(s) 30, 127
BstF5I GGATG 3 cut(s) 149, 443, 509
BstKTI GATC 7 cut(s) 60, 149, 212, 286, 346, 358, 402
BstMAI GTCTC 2 cut(s) 87, 566
BstMBI GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
BstMCI CGRYCG 2 cut(s) 96, 402
BstMWI GCNNNNNNNGC 2 cut(s) 75, 95
BstNI CCWGG 2 cut(s) 244, 467
BstPAI GACNNNNGTC 1 cut(s) 601
BstSCI CCNGG 2 cut(s) 242, 465
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 2 cut(s) 101, 391
BsuRI GGCC 1 cut(s) 439
BtsCI GGATG 3 cut(s) 149, 443, 509
BtsI GCAGTG 2 cut(s) 212, 621
BtsIMutI CAGTG 3 cut(s) 212, 493, 621
Cac8I GCNNGC 1 cut(s) 124
CciI TCATGA 3 cut(s) 60, 280, 352
Csp6I GTAC 1 cut(s) 27
CviAII CATG 6 cut(s) 61, 73, 275, 281, 287, 353
CviJI RGCY 6 cut(s) 69, 116, 247, 341, 382, 439
CviKI_1 RGCY 6 cut(s) 69, 116, 247, 341, 382, 439
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 2 cut(s) 30, 127
DpnI GATC 7 cut(s) 59, 148, 211, 285, 345, 357, 401
DpnII GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
EciI GGCGGA 1 cut(s) 436
Eco72I CACGTG 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 242, 465
FaeI CATG 6 cut(s) 64, 76, 278, 284, 290, 356
FatI CATG 6 cut(s) 60, 72, 274, 280, 286, 352
FbaI TGATCA 3 cut(s) 57, 283, 343
Fnu4HI GCNGC 2 cut(s) 90, 380
FokI GGATG 3 cut(s) 136, 430, 516
Fsp4HI GCNGC 2 cut(s) 90, 380
FspBI CTAG 4 cut(s) 24, 141, 156, 569
GluI GCNGC 2 cut(s) 90, 380
GsuI CTGGAG 1 cut(s) 226
HaeIII GGCC 1 cut(s) 439
HapII CCGG 2 cut(s) 86, 436
Hin1II CATG 6 cut(s) 64, 76, 278, 284, 290, 356
HinfI GANTC 1 cut(s) 428
HpaII CCGG 2 cut(s) 86, 436
HphI GGTGA 1 cut(s) 597
Hpy166II GTNNAC 1 cut(s) 321
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 4 cut(s) 61, 281, 353, 663
Hpy8I GTNNAC 1 cut(s) 321
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 3 cut(s) 34, 450, 536
HpyCH4III ACNGT 4 cut(s) 17, 414, 497, 589
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 1 cut(s) 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 95
HpyF3I CTNAG 2 cut(s) 30, 127
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 6 cut(s) 64, 76, 278, 284, 290, 356
Ksp22I TGATCA 3 cut(s) 57, 283, 343
Kzo9I GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
LmnI GCTCC 2 cut(s) 252, 338
LpnPI CCDG 9 cut(s) 92, 99, 229, 256, 337, 449, 452, 479, 495
Lsp1109I GCAGC 2 cut(s) 101, 391
LweI GCATC 1 cut(s) 109
MaeI CTAG 4 cut(s) 24, 141, 156, 569
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 3 cut(s) 547, 583, 603
MalI GATC 7 cut(s) 59, 148, 211, 285, 345, 357, 401
MboI GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
MboII GAAGA 6 cut(s) 49, 61, 156, 350, 353, 532
MluCI AATT 3 cut(s) 172, 184, 309
MseI TTAA 3 cut(s) 165, 183, 308
MslI CAYNNNNRTG 3 cut(s) 279, 285, 351
MspI CCGG 2 cut(s) 86, 436
MspR9I CCNGG 2 cut(s) 244, 467
MvaI CCWGG 2 cut(s) 244, 467
MwoI GCNNNNNNNGC 2 cut(s) 75, 95
NdeII GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
NlaIII CATG 6 cut(s) 64, 76, 278, 284, 290, 356
NlaIV GGNNCC 2 cut(s) 138, 248
NmuCI GTSAC 1 cut(s) 603
PagI TCATGA 3 cut(s) 60, 280, 352
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 2 cut(s) 91, 381
Ple19I CGATCG 1 cut(s) 402
PmaCI CACGTG 1 cut(s) 601
PmlI CACGTG 1 cut(s) 601
Ppu21I YACGTR 1 cut(s) 601
PshAI GACNNNNGTC 1 cut(s) 601
PshBI ATTAAT 2 cut(s) 183, 308
Psp6I CCWGG 2 cut(s) 242, 465
PspCI CACGTG 1 cut(s) 601
PspGI CCWGG 2 cut(s) 242, 465
PspN4I GGNNCC 2 cut(s) 138, 248
PstI CTGCAG 1 cut(s) 616
PvuI CGATCG 1 cut(s) 402
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
RseI CAYNNNNRTG 3 cut(s) 279, 285, 351
SaqAI TTAA 3 cut(s) 165, 183, 308
SatI GCNGC 2 cut(s) 90, 380
Sau3AI GATC 7 cut(s) 57, 146, 209, 283, 343, 355, 399
ScaI AGTACT 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 244, 467
SetI ASST 4 cut(s) 343, 471, 570, 603
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 612
SmiMI CAYNNNNRTG 3 cut(s) 279, 285, 351
SpeI ACTAGT 2 cut(s) 23, 155
Sse9I AATT 3 cut(s) 172, 184, 309
SsiI CCGC 2 cut(s) 96, 447
SspMI CTAG 4 cut(s) 24, 141, 156, 569
StyD4I CCNGG 2 cut(s) 242, 465
TaaI ACNGT 4 cut(s) 17, 414, 497, 589
TaiI ACGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 402, 564
TasI AATT 3 cut(s) 172, 184, 309
TatI WGTACW 1 cut(s) 26
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 3 cut(s) 165, 183, 308
Tru9I TTAA 3 cut(s) 165, 183, 308
TscAI CASTG 3 cut(s) 219, 500, 621
TseFI GTSAC 1 cut(s) 603
TseI GCWGC 2 cut(s) 89, 379
Tsp45I GTSAC 1 cut(s) 603
TspDTI ATGAA 4 cut(s) 17, 61, 269, 432
TspRI CASTG 3 cut(s) 219, 500, 621
VspI ATTAAT 2 cut(s) 183, 308
XspI CTAG 4 cut(s) 24, 141, 156, 569
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.