MD08G1221300.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014749
Physical Location & Seq
Reverse (-)
28389022 .. 28391829
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1221300.v1.1.491

Sequence Viewer

Length: 657 bp
ATGAACATGAAAAGCAGCAGCAGTACTAAGGAGAAGATGAAGAAAAAGATGATGATGAGGGGCTTCATGTGCCAGTCTCAAGCAGCAACTGCGGTGTGCATGTCTGATGCTCGCTCAGTTATCGTGCCACCGCCGACAAGACCAGCTGCAGCTACCATTGTCCGCCACAATCACAGTGCTGGCCATGTGAATTACATGATGATGAGAAGATCAACTAGTACTAATAATGGTGGCGGCGGTGGCGGTGGTCACTACTACTACTCCAGGCTCCTCGATACTACTCCTTCAGCTCATGGCCATGAGACGTCCCACTACCACAGTCGCAGACGAAGCGCTGGTAGTACCAGCAGTATTCTTAACCACCATGATCAGCGGCCAATGTCAAAGTCGACCCGAAAACTACCATCATCTGATCCAGATTTACTACCACCGTCGTCATCCGCCGACAATGTCTTCCAGGTGGTTGTTATGAGAGTTTCTATTCATTGCCATGGATGTGCTAGTAAATTGAAGAAACATCTCTCTAAAATGGAAGGGGTTACATCATTCAGTATAGACCTAGAAACAAAGATGGTAACTGTTAGGGGACACGTTTCACCAGTTGGAGTCGTGGAGAGCATTTCAAAAGTGAAGAAGGCAGAGTTATTAACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

219

Amino Acids

23.74

Weight (kDa)

10.13

Isoelectric Point (pI)

65.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 157 - 211 3.1e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 389
AciI CCGC 8 cut(s) 92, 131, 163, 234, 237, 243, 373, 441
AclWI GGATC 1 cut(s) 407
AcoI YGGCCR 3 cut(s) 181, 295, 374
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 3 cut(s) 25, 220, 343
AfeI AGCGCT 1 cut(s) 334
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 2 cut(s) 511, 624
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 2 cut(s) 263, 456
AluBI AGCT 3 cut(s) 146, 152, 290
AluI AGCT 3 cut(s) 146, 152, 290
Alw26I GTCTC 2 cut(s) 81, 296
AlwI GGATC 1 cut(s) 407
AlwNI CAGNNNCTG 1 cut(s) 89
Aor51HI AGCGCT 1 cut(s) 334
AoxI GGCC 3 cut(s) 181, 295, 374
ApeKI GCWGC 5 cut(s) 15, 18, 83, 146, 149
AspLEI GCGC 1 cut(s) 335
AsuHPI GGTGA 1 cut(s) 588
BalI TGGCCA 2 cut(s) 183, 297
BbsI GAAGAC 1 cut(s) 445
BbvI GCAGC 5 cut(s) 27, 30, 95, 133, 161
BccI CCATC 2 cut(s) 412, 565
BciT130I CCWGG 2 cut(s) 265, 458
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 81, 296
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 3 cut(s) 216, 501, 560
BfmI CTRYAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 7 cut(s) 16, 19, 84, 147, 150, 235, 374
BlsI GCNGC 7 cut(s) 17, 20, 85, 148, 151, 236, 375
BmcAI AGTACT 2 cut(s) 25, 220
Bme1390I CCNGG 2 cut(s) 265, 458
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 2 cut(s) 265, 458
BmsI GCATC 1 cut(s) 97
BpiI GAAGAC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 490
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bse1I ACTGG 3 cut(s) 73, 599, 651
Bse3DI GCAATG 1 cut(s) 484
BseBI CCWGG 2 cut(s) 265, 458
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 2 cut(s) 437, 500
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 3 cut(s) 73, 599, 651
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 5 cut(s) 27, 30, 95, 133, 161
BshFI GGCC 3 cut(s) 183, 297, 376
BslFI GGGAC 2 cut(s) 292, 600
BsmAI GTCTC 2 cut(s) 81, 296
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 2 cut(s) 292, 600
BsnI GGCC 3 cut(s) 183, 297, 376
Bsp143I GATC 3 cut(s) 209, 367, 412
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 8 cut(s) 92, 131, 163, 234, 237, 243, 373, 441
BspANI GGCC 3 cut(s) 183, 297, 376
BspCNI CTCAG 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 269
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 484
BsrI ACTGG 3 cut(s) 73, 599, 651
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 3 cut(s) 209, 367, 412
BssNI GRCGYC 1 cut(s) 305
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 2 cut(s) 265, 458
Bst4CI ACNGT 4 cut(s) 176, 320, 432, 580
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 112, 181
BstDEI CTNAG 2 cut(s) 27, 115
BstDSI CCRYGG 1 cut(s) 490
BstF5I GGATG 2 cut(s) 437, 500
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 3 cut(s) 212, 370, 415
BstMAI GTCTC 2 cut(s) 81, 296
BstMBI GATC 3 cut(s) 209, 367, 412
BstMWI GCNNNNNNNGC 4 cut(s) 69, 89, 240, 330
BstNI CCWGG 2 cut(s) 265, 458
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 263, 456
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 27, 30, 95, 133, 161
BstV2I GAAGAC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 183, 297, 376
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 2 cut(s) 437, 500
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 2 cut(s) 112, 181
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 335
Csp6I GTAC 3 cut(s) 24, 219, 342
CviAII CATG 9 cut(s) 7, 67, 100, 185, 196, 293, 299, 365, 491
CviJI RGCY 8 cut(s) 63, 146, 152, 183, 268, 290, 297, 376
CviKI_1 RGCY 8 cut(s) 63, 146, 152, 183, 268, 290, 297, 376
CviQI GTAC 3 cut(s) 24, 219, 342
DdeI CTNAG 2 cut(s) 27, 115
DpnI GATC 3 cut(s) 211, 369, 414
DpnII GATC 3 cut(s) 209, 367, 412
EaeI YGGCCR 3 cut(s) 181, 295, 374
EciI GGCGGA 2 cut(s) 152, 430
Eco130I CCWWGG 1 cut(s) 490
Eco47III AGCGCT 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 2 cut(s) 263, 456
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 9 cut(s) 10, 70, 103, 188, 199, 296, 302, 368, 494
FaqI GGGAC 2 cut(s) 292, 600
FatI CATG 9 cut(s) 6, 66, 99, 184, 195, 292, 298, 364, 490
FbaI TGATCA 1 cut(s) 367
FblI GTMKAC 1 cut(s) 389
Fnu4HI GCNGC 7 cut(s) 16, 19, 84, 147, 150, 235, 374
FokI GGATG 2 cut(s) 424, 507
Fsp4HI GCNGC 7 cut(s) 16, 19, 84, 147, 150, 235, 374
FspBI CTAG 3 cut(s) 216, 501, 560
GlaI GCGC 1 cut(s) 334
GluI GCNGC 7 cut(s) 16, 19, 84, 147, 150, 235, 374
GsuI CTGGAG 1 cut(s) 247
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 3 cut(s) 183, 297, 376
HhaI GCGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 9 cut(s) 10, 70, 103, 188, 199, 296, 302, 368, 494
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 1 cut(s) 606
HphI GGTGA 1 cut(s) 588
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 2 cut(s) 106, 412
Hpy188III TCNNGA 1 cut(s) 416
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 3 cut(s) 294, 527, 628
HpyCH4III ACNGT 4 cut(s) 176, 320, 432, 580
HpyCH4IV ACGT 2 cut(s) 305, 591
HpyCH4V TGCA 2 cut(s) 99, 149
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 89, 240, 330
HpyF3I CTNAG 2 cut(s) 27, 115
HpySE526I ACGT 2 cut(s) 305, 591
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 9 cut(s) 10, 70, 103, 188, 199, 296, 302, 368, 494
HspAI GCGC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 3 cut(s) 209, 367, 412
LmnI GCTCC 1 cut(s) 273
Lsp1109I GCAGC 5 cut(s) 27, 30, 95, 133, 161
LweI GCATC 1 cut(s) 97
MaeI CTAG 3 cut(s) 216, 501, 560
MaeII ACGT 2 cut(s) 305, 591
MaeIII GTNAC 3 cut(s) 248, 538, 574
MalI GATC 3 cut(s) 211, 369, 414
MboI GATC 3 cut(s) 209, 367, 412
MboII GAAGA 6 cut(s) 46, 52, 219, 445, 523, 643
MlsI TGGCCA 2 cut(s) 183, 297
MluCI AATT 2 cut(s) 190, 506
MluNI TGGCCA 2 cut(s) 183, 297
MlyI GAGTC 1 cut(s) 615
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 2 cut(s) 51, 281
Mox20I TGGCCA 2 cut(s) 183, 297
MscI TGGCCA 2 cut(s) 183, 297
MseI TTAA 2 cut(s) 357, 647
MslI CAYNNNNRTG 4 cut(s) 200, 297, 489, 495
Msp20I TGGCCA 2 cut(s) 183, 297
MspA1I CMGCKG 2 cut(s) 146, 373
MspR9I CCNGG 2 cut(s) 265, 458
MvaI CCWGG 2 cut(s) 265, 458
MwoI GCNNNNNNNGC 4 cut(s) 69, 89, 240, 330
NcoI CCATGG 1 cut(s) 490
NdeII GATC 3 cut(s) 209, 367, 412
NlaIII CATG 9 cut(s) 10, 70, 103, 188, 199, 296, 302, 368, 494
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 248
NspI RCATGY 1 cut(s) 103
PflFI GACNNNGTC 1 cut(s) 449
PkrI GCNGC 7 cut(s) 17, 20, 85, 148, 151, 236, 375
PleI GAGTC 1 cut(s) 614
PpsI GAGTC 1 cut(s) 614
Psp6I CCWGG 2 cut(s) 263, 456
PspGI CCWGG 2 cut(s) 263, 456
PspN4I GGNNCC 1 cut(s) 269
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 89
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 3 cut(s) 25, 220, 343
RsaNI GTAC 3 cut(s) 24, 219, 342
RseI CAYNNNNRTG 4 cut(s) 200, 297, 489, 495
SalI GTCGAC 1 cut(s) 388
SaqAI TTAA 2 cut(s) 357, 647
SatI GCNGC 7 cut(s) 16, 19, 84, 147, 150, 235, 374
Sau3AI GATC 3 cut(s) 209, 367, 412
ScaI AGTACT 2 cut(s) 25, 220
SchI GAGTC 1 cut(s) 615
ScrFI CCNGG 2 cut(s) 265, 458
SetI ASST 7 cut(s) 148, 154, 292, 308, 462, 561, 594
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 147
SmiMI CAYNNNNRTG 4 cut(s) 200, 297, 489, 495
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 2 cut(s) 190, 506
SsiI CCGC 8 cut(s) 92, 131, 163, 234, 237, 243, 373, 441
SspMI CTAG 3 cut(s) 216, 501, 560
StyD4I CCNGG 2 cut(s) 263, 456
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 4 cut(s) 176, 320, 432, 580
TaiI ACGT 2 cut(s) 308, 594
TaqI TCGA 2 cut(s) 273, 389
TasI AATT 2 cut(s) 190, 506
TatI WGTACW 2 cut(s) 23, 218
TauI GCSGC 2 cut(s) 237, 376
Tru1I TTAA 2 cut(s) 357, 647
Tru9I TTAA 2 cut(s) 357, 647
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 5 cut(s) 15, 18, 83, 146, 149
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 5 cut(s) 17, 23, 53, 55, 473
TspRI CASTG 1 cut(s) 181
Tth111I GACNNNGTC 1 cut(s) 449
XceI RCATGY 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 389
XspI CTAG 3 cut(s) 216, 501, 560
ZraI GACGTC 1 cut(s) 306
ZrmI AGTACT 2 cut(s) 25, 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.