Rw7G032000

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014749
Physical Location & Seq
Forward (+)
46255417 .. 46258644
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G032000.1

Sequence Viewer

Length: 567 bp
ATGATGAACATGAAGAGCAGCACTGCTACTAAGAAGAAGATGACGATGATGCCGAAGGGGTTCATGTGCCACTCTCAGGCAGCAACTGCCGTCTGCGTGAGCAGCGCCGATGCAGCTCGGTCCGCGATCGTCCCAGCTGCTAGAAATACGAGAATGATCAACAACTACAATGCCAGGTACTCGAAGATCGTTGACTCACGTAGGTCTGGCAATTTAGCTGATCATGGACAAAAACCAGTCTTGTTGTCTTCCATGATCAAGAGAGGGTCAGCTTTGAACTACCACCACCACCATCATCACTCGAAGCCAAAGATGTCACTGCAGAAAACAGCGTCAGTTCTACCTTCTACAGATAATGTCTTCCAGGTGGTTGTGATGAGGGTTGCTCTTCATTGTCAAGGATGTGCTGGTAAAGTGAAGAAACATCTTTCTAAAATGGAAGGGGTTACATCTTTCAGTATAGACCTGGAGACAAAGATGGTAACTGTAAGAGGACACGTGTCGCCAGTCAGAGTCGTGGAGAGCATTTCCAAGGTTAAAAAGGCAGAGTTACTGCCCTGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

188

Amino Acids

20.59

Weight (kDa)

10.25

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 126 - 180 3.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 123
AcvI CACGTG 1 cut(s) 499
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 2 cut(s) 496, 498
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 3 cut(s) 173, 363, 465
AluBI AGCT 4 cut(s) 116, 137, 218, 272
AluI AGCT 4 cut(s) 116, 137, 218, 272
Alw26I GTCTC 1 cut(s) 464
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 5 cut(s) 18, 80, 102, 113, 137
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 120
AvaII GGWCC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 499
BbsI GAAGAC 2 cut(s) 240, 352
BbvI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BccI CCATC 2 cut(s) 300, 472
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 175, 365, 467
BclI TGATCA 3 cut(s) 156, 220, 255
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 3 cut(s) 320, 348, 559
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 5 cut(s) 19, 81, 103, 114, 138
BlsI GCNGC 5 cut(s) 20, 82, 104, 115, 139
Bme1390I CCNGG 3 cut(s) 175, 365, 467
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 175, 365, 467
BmsI GCATC 2 cut(s) 39, 100
BoxI GACNNNNGTC 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 240, 352
BplI GAGNNNNNCTC 2 cut(s) 370, 402
BpmI CTGGAG 1 cut(s) 488
BsaAI YACGTR 2 cut(s) 200, 499
BsaJI CCNNGG 1 cut(s) 531
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 236, 506
BseBI CCWGG 3 cut(s) 175, 365, 467
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 1 cut(s) 407
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 236, 506
BseXI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 116
Bsp143I GATC 5 cut(s) 126, 156, 186, 220, 255
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 125
BspMAI CTGCAG 2 cut(s) 324, 563
BspQI GCTCTTC 2 cut(s) 8, 393
BsrI ACTGG 2 cut(s) 236, 506
BssECI CCNNGG 1 cut(s) 531
BssMI GATC 5 cut(s) 126, 156, 186, 220, 255
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 3 cut(s) 175, 365, 467
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 2 cut(s) 8, 393
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBAI YACGTR 2 cut(s) 200, 499
BstDEI CTNAG 2 cut(s) 30, 75
BstF5I GGATG 1 cut(s) 407
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 5 cut(s) 129, 159, 189, 223, 258
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 126, 156, 186, 220, 255
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
BstNI CCWGG 3 cut(s) 175, 365, 467
BstPAI GACNNNNGTC 1 cut(s) 499
BstSCI CCNGG 3 cut(s) 173, 363, 465
BstSFI CTRYAG 3 cut(s) 320, 348, 559
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 5 cut(s) 30, 92, 114, 124, 125
BstV2I GAAGAC 2 cut(s) 240, 352
BtsCI GGATG 1 cut(s) 407
BtsI GCAGTG 2 cut(s) 21, 317
BtsIMutI CAGTG 2 cut(s) 21, 317
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 1 cut(s) 321
Csp6I GTAC 1 cut(s) 178
CspI CGGWCCG 1 cut(s) 120
CviAII CATG 4 cut(s) 10, 64, 224, 253
CviJI RGCY 5 cut(s) 116, 137, 218, 272, 307
CviKI_1 RGCY 5 cut(s) 116, 137, 218, 272, 307
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 30, 75
DpnI GATC 5 cut(s) 128, 158, 188, 222, 257
DpnII GATC 5 cut(s) 126, 156, 186, 220, 255
Eam1104I CTCTTC 2 cut(s) 8, 393
EarI CTCTTC 2 cut(s) 8, 393
Eco130I CCWWGG 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 120
Eco72I CACGTG 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 173, 363, 465
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 4 cut(s) 13, 67, 227, 256
FaiI YATR 5 cut(s) 11, 65, 225, 254, 461
FaqI GGGAC 1 cut(s) 116
FatI CATG 4 cut(s) 9, 63, 223, 252
FbaI TGATCA 3 cut(s) 156, 220, 255
Fnu4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FspBI CTAG 1 cut(s) 141
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 19, 81, 103, 114, 138
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 108
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 13, 67, 227, 256
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 2 cut(s) 194, 513
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 1 cut(s) 512
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 49, 354, 434
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 2 cut(s) 199, 498
HpyCH4V TGCA 3 cut(s) 113, 322, 561
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
HpyF3I CTNAG 2 cut(s) 30, 75
HpySE526I ACGT 2 cut(s) 199, 498
Hsp92II CATG 4 cut(s) 13, 67, 227, 256
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 3 cut(s) 156, 220, 255
Kzo9I GATC 5 cut(s) 126, 156, 186, 220, 255
LguI GCTCTTC 2 cut(s) 8, 393
Lsp1109I GCAGC 5 cut(s) 30, 92, 114, 124, 125
LweI GCATC 2 cut(s) 39, 100
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 199, 498
MaeIII GTNAC 4 cut(s) 315, 445, 481, 549
MalI GATC 5 cut(s) 128, 158, 188, 222, 257
MboI GATC 5 cut(s) 126, 156, 186, 220, 255
MboII GAAGA 8 cut(s) 25, 46, 49, 196, 240, 352, 380, 430
MluCI AATT 1 cut(s) 211
MlyI GAGTC 2 cut(s) 188, 522
MnlI CCTC 3 cut(s) 257, 372, 485
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 175, 365, 467
MvaI CCWGG 3 cut(s) 175, 365, 467
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
NdeII GATC 5 cut(s) 126, 156, 186, 220, 255
NlaIII CATG 4 cut(s) 13, 67, 227, 256
NmuCI GTSAC 1 cut(s) 315
PciSI GCTCTTC 2 cut(s) 8, 393
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 59
PkrI GCNGC 5 cut(s) 20, 82, 104, 115, 139
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 2 cut(s) 188, 521
PmaCI CACGTG 1 cut(s) 499
PmlI CACGTG 1 cut(s) 499
PpsI GAGTC 2 cut(s) 188, 521
Ppu21I YACGTR 2 cut(s) 200, 499
PshAI GACNNNNGTC 1 cut(s) 499
Psp6I CCWGG 3 cut(s) 173, 363, 465
PspCI CACGTG 1 cut(s) 499
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 173, 363, 465
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 2 cut(s) 324, 563
PstNI CAGNNNCTG 1 cut(s) 86
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SapI GCTCTTC 2 cut(s) 8, 393
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 5 cut(s) 19, 81, 103, 114, 138
Sau3AI GATC 5 cut(s) 126, 156, 186, 220, 255
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 2 cut(s) 188, 522
ScrFI CCNGG 3 cut(s) 175, 365, 467
SfaNI GCATC 2 cut(s) 39, 100
SfcI CTRYAG 3 cut(s) 320, 348, 559
SinI GGWCC 1 cut(s) 120
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 173, 363, 465
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 2 cut(s) 202, 501
TaqI TCGA 2 cut(s) 182, 302
TaqII GACCGA 1 cut(s) 108
TasI AATT 1 cut(s) 211
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 28, 324
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 5 cut(s) 18, 80, 102, 113, 137
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 4 cut(s) 20, 26, 52, 380
TspRI CASTG 2 cut(s) 28, 324
VpaK11BI GGWCC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.