Rroxscaffold_3G00233160

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014749
Physical Location & Seq
Reverse (-)
19061142 .. 19064548
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233160.1

Sequence Viewer

Length: 561 bp
ATGATGAACATGAAGAGCAGCAGTTCTACTAAGAAGAAGATGACGATGATGCCAAAGGGGTTCATGTGCCACTCTCAGGCAGCAACTGCAGTGTGCGTGAGCAGCGCCGATGCAGCTCGGTCCGCGATCGTCCCAGCTGCTAGAAATACAAGAATGATCAACAACTACAATGCCAGGTATTCGAAGATCGTTGAGTCACGTAGGTCTGGCAATTTAGCTGATCATGGAAAAAAACCAGTCTTGCTGTCTTCCATGATCAAGAGAGAGTCGGCTTTGAACTACCACCATCATCACTCGAAGCCAAAGATGTCACTGCAGAAAACAGCGTCAGTTCTACCTTCTACAGATAATGTCTTCCAGGTGGTTGTTATGAGGGTTGCTCTTCATTGTCAAGGATGTGCTGGTAAAGTGAAGAAACATCTTTCTAAAATGGAAGGGGTTACATCCTTCAGTATAGACCTGGAGACAAAGATGGTAACTGTAAGAGGACACGTGTCGCCAGTCAGAGTCGTGGAGAGCATTTCCAAGGTTAAAAGGGCAGAGTTACTGCCCTGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

186

Amino Acids

20.43

Weight (kDa)

10.25

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 124 - 176 3.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 123
AcuI CTGAAG 1 cut(s) 433
AcvI CACGTG 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 2 cut(s) 490, 492
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 3 cut(s) 173, 357, 459
AluBI AGCT 3 cut(s) 116, 137, 218
AluI AGCT 3 cut(s) 116, 137, 218
Alw26I GTCTC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 5 cut(s) 18, 80, 102, 113, 137
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 120
AsuII TTCGAA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 493
BbsI GAAGAC 2 cut(s) 240, 346
BbvI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BccI CCATC 2 cut(s) 294, 466
BciT130I CCWGG 3 cut(s) 175, 359, 461
BclI TGATCA 3 cut(s) 156, 220, 255
BcoDI GTCTC 1 cut(s) 458
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 4 cut(s) 87, 314, 342, 553
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 5 cut(s) 19, 81, 103, 114, 138
BlsI GCNGC 5 cut(s) 20, 82, 104, 115, 139
Bme1390I CCNGG 3 cut(s) 175, 359, 461
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 175, 359, 461
BmsI GCATC 2 cut(s) 39, 100
BoxI GACNNNNGTC 1 cut(s) 493
BpiI GAAGAC 2 cut(s) 240, 346
BplI GAGNNNNNCTC 2 cut(s) 364, 396
BpmI CTGGAG 1 cut(s) 482
Bpu14I TTCGAA 1 cut(s) 182
BsaAI YACGTR 2 cut(s) 200, 493
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 236, 500
BseBI CCWGG 3 cut(s) 175, 359, 461
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 2 cut(s) 401, 443
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 236, 500
BseXI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 125
Bsh1285I CGRYCG 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 182
Bsp143I GATC 5 cut(s) 126, 156, 186, 220, 255
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 125
BspMAI CTGCAG 3 cut(s) 91, 318, 557
BspQI GCTCTTC 2 cut(s) 8, 387
BspT104I TTCGAA 1 cut(s) 182
BsrI ACTGG 2 cut(s) 236, 500
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 5 cut(s) 126, 156, 186, 220, 255
BssT1I CCWWGG 1 cut(s) 525
Bst2UI CCWGG 3 cut(s) 175, 359, 461
Bst4CI ACNGT 1 cut(s) 481
Bst6I CTCTTC 2 cut(s) 8, 387
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBAI YACGTR 2 cut(s) 200, 493
BstBI TTCGAA 1 cut(s) 182
BstDEI CTNAG 2 cut(s) 30, 75
BstF5I GGATG 2 cut(s) 401, 443
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 5 cut(s) 129, 159, 189, 223, 258
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 5 cut(s) 126, 156, 186, 220, 255
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
BstNI CCWGG 3 cut(s) 175, 359, 461
BstPAI GACNNNNGTC 1 cut(s) 493
BstSCI CCNGG 3 cut(s) 173, 357, 459
BstSFI CTRYAG 4 cut(s) 87, 314, 342, 553
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 5 cut(s) 30, 92, 114, 124, 125
BstV2I GAAGAC 2 cut(s) 240, 346
BtsCI GGATG 2 cut(s) 401, 443
BtsI GCAGTG 2 cut(s) 96, 311
BtsIMutI CAGTG 2 cut(s) 96, 311
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 1 cut(s) 315
CspI CGGWCCG 1 cut(s) 120
CviAII CATG 4 cut(s) 10, 64, 224, 253
CviJI RGCY 5 cut(s) 116, 137, 218, 272, 301
CviKI_1 RGCY 5 cut(s) 116, 137, 218, 272, 301
DdeI CTNAG 2 cut(s) 30, 75
DpnI GATC 5 cut(s) 128, 158, 188, 222, 257
DpnII GATC 5 cut(s) 126, 156, 186, 220, 255
Eam1104I CTCTTC 2 cut(s) 8, 387
EarI CTCTTC 2 cut(s) 8, 387
Eco130I CCWWGG 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 433
Eco72I CACGTG 1 cut(s) 493
EcoRII CCWGG 3 cut(s) 173, 357, 459
EcoT14I CCWWGG 1 cut(s) 525
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 4 cut(s) 13, 67, 227, 256
FaiI YATR 6 cut(s) 11, 65, 225, 254, 371, 455
FaqI GGGAC 1 cut(s) 116
FatI CATG 4 cut(s) 9, 63, 223, 252
FbaI TGATCA 3 cut(s) 156, 220, 255
Fnu4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FokI GGATG 2 cut(s) 408, 430
Fsp4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FspBI CTAG 1 cut(s) 141
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 19, 81, 103, 114, 138
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 482
HaeII RGCGCY 1 cut(s) 108
HgaI GACGC 1 cut(s) 315
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 13, 67, 227, 256
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 194, 266, 507
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 348, 428, 457
HpyCH4III ACNGT 1 cut(s) 481
HpyCH4IV ACGT 2 cut(s) 199, 492
HpyCH4V TGCA 4 cut(s) 89, 113, 316, 555
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
HpyF3I CTNAG 2 cut(s) 30, 75
HpySE526I ACGT 2 cut(s) 199, 492
Hsp92II CATG 4 cut(s) 13, 67, 227, 256
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 3 cut(s) 156, 220, 255
Kzo9I GATC 5 cut(s) 126, 156, 186, 220, 255
LguI GCTCTTC 2 cut(s) 8, 387
Lsp1109I GCAGC 5 cut(s) 30, 92, 114, 124, 125
LweI GCATC 2 cut(s) 39, 100
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 199, 492
MaeIII GTNAC 5 cut(s) 195, 309, 439, 475, 543
MalI GATC 5 cut(s) 128, 158, 188, 222, 257
MboI GATC 5 cut(s) 126, 156, 186, 220, 255
MboII GAAGA 8 cut(s) 25, 46, 49, 196, 240, 346, 374, 424
MluCI AATT 1 cut(s) 211
MlyI GAGTC 3 cut(s) 203, 275, 516
MnlI CCTC 2 cut(s) 366, 479
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 175, 359, 461
MvaI CCWGG 3 cut(s) 175, 359, 461
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 4 cut(s) 86, 102, 113, 122
NdeII GATC 5 cut(s) 126, 156, 186, 220, 255
NlaIII CATG 4 cut(s) 13, 67, 227, 256
NmuCI GTSAC 2 cut(s) 195, 309
NspV TTCGAA 1 cut(s) 182
PciSI GCTCTTC 2 cut(s) 8, 387
PkrI GCNGC 5 cut(s) 20, 82, 104, 115, 139
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 3 cut(s) 202, 274, 515
PmaCI CACGTG 1 cut(s) 493
PmlI CACGTG 1 cut(s) 493
PpsI GAGTC 3 cut(s) 202, 274, 515
Ppu21I YACGTR 2 cut(s) 200, 493
PshAI GACNNNNGTC 1 cut(s) 493
Psp6I CCWGG 3 cut(s) 173, 357, 459
PspCI CACGTG 1 cut(s) 493
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 173, 357, 459
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 3 cut(s) 91, 318, 557
PstNI CAGNNNCTG 1 cut(s) 86
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 137
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SapI GCTCTTC 2 cut(s) 8, 387
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 5 cut(s) 19, 81, 103, 114, 138
Sau3AI GATC 5 cut(s) 126, 156, 186, 220, 255
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 3 cut(s) 203, 275, 516
ScrFI CCNGG 3 cut(s) 175, 359, 461
SfaNI GCATC 2 cut(s) 39, 100
SfcI CTRYAG 4 cut(s) 87, 314, 342, 553
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 120
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 173, 357, 459
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 481
TaiI ACGT 2 cut(s) 202, 495
TaqI TCGA 2 cut(s) 182, 296
TaqII GACCGA 1 cut(s) 108
TasI AATT 1 cut(s) 211
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 96, 318
TseFI GTSAC 2 cut(s) 195, 309
TseI GCWGC 5 cut(s) 18, 80, 102, 113, 137
Tsp45I GTSAC 2 cut(s) 195, 309
TspDTI ATGAA 4 cut(s) 20, 26, 52, 374
TspRI CASTG 2 cut(s) 96, 318
VpaK11BI GGWCC 1 cut(s) 120
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.