RLG00000001761

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014749
Physical Location & Seq
Reverse (-)
20302847 .. 20305106
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001761

Sequence Viewer

Length: 570 bp
ATGATGAACATGAAGAGCAGCACTGCTACTAAGAAGAAGATGACGATGATGCCGAGGGGGTTCATGTGCCACTCTCAGGCAGCAACTGCCGTCTGTGTGAGCAGCGCCGATGCAGCTCGGTCCGCTATCGTCCCAGCTGCTAGAAATACGAGAATGATCAACAACTACAATGCCAGGTACTCGAAGATCGTTGAGTCACGTAGGTCTGGCAATTTAGCTGATCATGGAAAAAAACCAGTCTTGTTGTCTTCCATGATCAAGAGAGAGTCGGCTTTGAACTACCACCACCACCACCATCATCACTCGAAGCCAAAGATGTCACTGCAGAAAACAGCGTCAGTTCTACCTTCTACAGATAATGTCTTCCAGGTGGTTGTTATGAGGGTTGCTCTTCATTGTCAAGGATGTGCTGGTAAAGTAAAGAAACATCTTTCGAAAATGGAAGGGGTTACATCTTTCAGTATAGACCTGGAGACAAAGATGGTAACTGTAAGAGGACACGTGTCACCAGTCAGAGTCGTGGAGAGCATTTCCAAGGTTAAAAAGGCAGAGTTACTGCCCTGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

190

Amino Acids

20.84

Weight (kDa)

10.25

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 127 - 181 3.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014749)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g30890
malus_domestica MD08G1221300.v1.1 MD15G1412000.v1.1
prunus_persica Prupe.1G558400_v2.0.a1 Prupe.1G558400_v2.0.a1
pyrus_communis pycom08g19190 pycom15g36680
rosa_chinensis RchiOBHm_Chr7g0226171
rosa_laevigata RLG00000001761
rosa_multiflora Rmu_sc0001350.1_g000012
rosa_roxburghii Rroxscaffold_3G00233160
rosa_rugosa Rorug07G0235200
rosa_samantha Rh7AG382200 Rh7BG369200 Rh7CG401700 Rh7DG380600
rosa_wichuraiana Rw7G032000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 123
AcvI CACGTG 1 cut(s) 502
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 76
AflIII ACRYGT 2 cut(s) 499, 501
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 3 cut(s) 173, 366, 468
AluBI AGCT 3 cut(s) 116, 137, 218
AluI AGCT 3 cut(s) 116, 137, 218
Alw26I GTCTC 1 cut(s) 467
AlwNI CAGNNNCTG 1 cut(s) 86
ApeKI GCWGC 5 cut(s) 18, 80, 102, 113, 137
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 498
AsuII TTCGAA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 502
BbsI GAAGAC 2 cut(s) 240, 355
BbvI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BccI CCATC 2 cut(s) 303, 475
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 175, 368, 470
BclI TGATCA 3 cut(s) 156, 220, 255
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 3 cut(s) 323, 351, 562
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 5 cut(s) 19, 81, 103, 114, 138
BlsI GCNGC 5 cut(s) 20, 82, 104, 115, 139
Bme1390I CCNGG 3 cut(s) 175, 368, 470
Bme18I GGWCC 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 120
BmrFI CCNGG 3 cut(s) 175, 368, 470
BmsI GCATC 2 cut(s) 39, 100
BoxI GACNNNNGTC 1 cut(s) 502
BpiI GAAGAC 2 cut(s) 240, 355
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 1 cut(s) 491
Bpu14I TTCGAA 1 cut(s) 434
BsaAI YACGTR 2 cut(s) 200, 502
BsaJI CCNNGG 2 cut(s) 53, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 236, 509
BseBI CCWGG 3 cut(s) 175, 368, 470
BseDI CCNNGG 2 cut(s) 53, 534
BseGI GGATG 1 cut(s) 410
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 236, 509
BseXI GCAGC 5 cut(s) 30, 92, 114, 124, 125
BseYI CCCAGC 1 cut(s) 133
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 434
Bsp143I GATC 4 cut(s) 156, 186, 220, 255
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 88
BspMAI CTGCAG 2 cut(s) 327, 566
BspQI GCTCTTC 2 cut(s) 8, 396
BspT104I TTCGAA 1 cut(s) 434
BsrI ACTGG 2 cut(s) 236, 509
BssECI CCNNGG 2 cut(s) 53, 534
BssMI GATC 4 cut(s) 156, 186, 220, 255
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 3 cut(s) 175, 368, 470
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 2 cut(s) 8, 396
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBAI YACGTR 2 cut(s) 200, 502
BstBI TTCGAA 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 30, 75
BstF5I GGATG 1 cut(s) 410
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 4 cut(s) 159, 189, 223, 258
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 4 cut(s) 156, 186, 220, 255
BstMWI GCNNNNNNNGC 3 cut(s) 86, 113, 122
BstNI CCWGG 3 cut(s) 175, 368, 470
BstPAI GACNNNNGTC 1 cut(s) 502
BstSCI CCNGG 3 cut(s) 173, 366, 468
BstSFI CTRYAG 3 cut(s) 323, 351, 562
BstV1I GCAGC 5 cut(s) 30, 92, 114, 124, 125
BstV2I GAAGAC 2 cut(s) 240, 355
BtsCI GGATG 1 cut(s) 410
BtsI GCAGTG 2 cut(s) 21, 320
BtsIMutI CAGTG 2 cut(s) 21, 320
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 120
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 1 cut(s) 324
Csp6I GTAC 1 cut(s) 178
CspI CGGWCCG 1 cut(s) 120
CviAII CATG 4 cut(s) 10, 64, 224, 253
CviJI RGCY 5 cut(s) 116, 137, 218, 272, 310
CviKI_1 RGCY 5 cut(s) 116, 137, 218, 272, 310
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 30, 75
DpnI GATC 4 cut(s) 158, 188, 222, 257
DpnII GATC 4 cut(s) 156, 186, 220, 255
Eam1104I CTCTTC 2 cut(s) 8, 396
EarI CTCTTC 2 cut(s) 8, 396
Eco130I CCWWGG 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 120
Eco72I CACGTG 1 cut(s) 502
EcoRII CCWGG 3 cut(s) 173, 366, 468
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 4 cut(s) 13, 67, 227, 256
FaiI YATR 6 cut(s) 11, 65, 225, 254, 380, 464
FaqI GGGAC 1 cut(s) 116
FatI CATG 4 cut(s) 9, 63, 223, 252
FbaI TGATCA 3 cut(s) 156, 220, 255
Fnu4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FokI GGATG 1 cut(s) 417
Fsp4HI GCNGC 5 cut(s) 19, 81, 103, 114, 138
FspBI CTAG 1 cut(s) 141
GlaI GCGC 1 cut(s) 106
GluI GCNGC 5 cut(s) 19, 81, 103, 114, 138
GsaI CCCAGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 491
HaeII RGCGCY 1 cut(s) 108
HgaI GACGC 1 cut(s) 324
HhaI GCGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 13, 67, 227, 256
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HinfI GANTC 3 cut(s) 194, 266, 516
HphI GGTGA 1 cut(s) 498
Hpy188I TCNGA 1 cut(s) 515
Hpy188III TCNNGA 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 357, 437
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 2 cut(s) 199, 501
HpyCH4V TGCA 3 cut(s) 113, 325, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 113, 122
HpyF3I CTNAG 2 cut(s) 30, 75
HpySE526I ACGT 2 cut(s) 199, 501
Hsp92II CATG 4 cut(s) 13, 67, 227, 256
HspAI GCGC 1 cut(s) 105
Ksp22I TGATCA 3 cut(s) 156, 220, 255
Kzo9I GATC 4 cut(s) 156, 186, 220, 255
LguI GCTCTTC 2 cut(s) 8, 396
Lsp1109I GCAGC 5 cut(s) 30, 92, 114, 124, 125
LweI GCATC 2 cut(s) 39, 100
MaeI CTAG 1 cut(s) 141
MaeII ACGT 2 cut(s) 199, 501
MaeIII GTNAC 6 cut(s) 195, 318, 448, 484, 504, 552
MalI GATC 4 cut(s) 158, 188, 222, 257
MboI GATC 4 cut(s) 156, 186, 220, 255
MboII GAAGA 7 cut(s) 25, 46, 49, 196, 240, 355, 383
MluCI AATT 1 cut(s) 211
MlyI GAGTC 3 cut(s) 203, 275, 525
MnlI CCTC 3 cut(s) 48, 375, 488
MseI TTAA 1 cut(s) 540
MspA1I CMGCKG 1 cut(s) 137
MspR9I CCNGG 3 cut(s) 175, 368, 470
MvaI CCWGG 3 cut(s) 175, 368, 470
MwoI GCNNNNNNNGC 3 cut(s) 86, 113, 122
NdeII GATC 4 cut(s) 156, 186, 220, 255
NlaIII CATG 4 cut(s) 13, 67, 227, 256
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 3 cut(s) 195, 318, 504
NspV TTCGAA 1 cut(s) 434
PciSI GCTCTTC 2 cut(s) 8, 396
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PkrI GCNGC 5 cut(s) 20, 82, 104, 115, 139
PleI GAGTC 3 cut(s) 202, 274, 524
PmaCI CACGTG 1 cut(s) 502
PmlI CACGTG 1 cut(s) 502
PpsI GAGTC 3 cut(s) 202, 274, 524
Ppu21I YACGTR 2 cut(s) 200, 502
PshAI GACNNNNGTC 1 cut(s) 502
Psp6I CCWGG 3 cut(s) 173, 366, 468
PspCI CACGTG 1 cut(s) 502
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 173, 366, 468
PspPI GGNCC 1 cut(s) 120
PstI CTGCAG 2 cut(s) 327, 566
PstNI CAGNNNCTG 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SapI GCTCTTC 2 cut(s) 8, 396
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 5 cut(s) 19, 81, 103, 114, 138
Sau3AI GATC 4 cut(s) 156, 186, 220, 255
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 3 cut(s) 203, 275, 525
ScrFI CCNGG 3 cut(s) 175, 368, 470
SfaNI GCATC 2 cut(s) 39, 100
SfcI CTRYAG 3 cut(s) 323, 351, 562
SfuI TTCGAA 1 cut(s) 434
SinI GGWCC 1 cut(s) 120
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 3 cut(s) 173, 366, 468
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 2 cut(s) 202, 504
TaqI TCGA 3 cut(s) 182, 305, 434
TaqII GACCGA 1 cut(s) 108
TasI AATT 1 cut(s) 211
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 2 cut(s) 28, 327
TseFI GTSAC 3 cut(s) 195, 318, 504
TseI GCWGC 5 cut(s) 18, 80, 102, 113, 137
Tsp45I GTSAC 3 cut(s) 195, 318, 504
TspDTI ATGAA 4 cut(s) 20, 26, 52, 383
TspRI CASTG 2 cut(s) 28, 327
VpaK11BI GGWCC 1 cut(s) 120
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.