FvH4_5g31600

ATP-dependent protease La (LON) substrate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003413
Physical Location & Seq
Forward (+)
22766068 .. 22770607
4540 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31600.t1

Sequence Viewer

Length: 882 bp
ATGACTTGTACGGCCTTCAATCTCACCACCAGCCCCCTTCTCCCTTCAAGACCGACCCGACTCCGAAACCCGCACCCGACCCTCCATCCTTTCCGTTTCACCCCCAAGCCGAAACGATGCCGTTTCTCCCCAAACGCCACCTCCTTGGACCTCCCGCTCCTCCCCTTCACCTTGACCGAGGTTCTGGTTCCATCTGAGTGTAAAACGCTTCATCTATATGAAGCAAGATACCTTGCTCTACTAGAGGAGTCATTGTCGAAGAGCAAGAAGTTCTTTGTGCATTTTGTGCTGGATCCTATCATTGTTGATGATTCATTGGAAGAAGCATCGTTTGCAGCCAGAAATGGTTGTTTGGTATTTATAGAGAATGTTGAGAGATTAGAAGTGGGAGCATTAGTCTCTATAAGAGGAATCGGTCGTGTAAAGATTATCAAATTCTTGCAAGCAGATCCGTACTTGAAAGGTGTTGTCATACCCGTGCACGACACGCTACCTGACAACATAAGCAGATTAAACCCCAAAGTTATGCAAGTAAAAGAGGCTCTATGCAGTTTAAATAGTTTGGAAATCAAACTAAAGGCTCCAAAGGAGGCACCTTTGCAGACTCAAATTGCCAACTCTCTGATGTGGGCGGAAAAGGAACTACAAGTGGACTGTGATGAAACTTTCTTTCCTTCCTTGGCTGAGCGTGTATCCTTTGCGGCACTTCAGCCTGTATCAGGATCAAGTCAATCAGAACTTCTGAAACTGCAGCAAGAGAAATTAAAGGCAATGGAGCTGAAAGACACAATGCATAGGCTAGATAGTTCATTACAATTCGTCAAAGATAATATCTCCATGGTTGCAGCCAAGCTTGCTATACAATCAGTGGAGATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

294

Amino Acids

32.9

Weight (kDa)

8.46

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 52 - 183 6.7e-10 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 157
AciI CCGC 4 cut(s) 71, 155, 632, 701
AclWI GGATC 4 cut(s) 287, 300, 443, 730
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 1 cut(s) 692
AfaI GTAC 2 cut(s) 10, 455
AfiI CCNNNNNNNGG 1 cut(s) 719
AgsI TTSAA 3 cut(s) 19, 48, 460
AluBI AGCT 2 cut(s) 778, 853
AluI AGCT 2 cut(s) 778, 853
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 403
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 4 cut(s) 287, 300, 443, 730
AoxI GGCC 1 cut(s) 12
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 3 cut(s) 335, 751, 845
ApoI RAATTY 1 cut(s) 434
AspS9I GGNCC 1 cut(s) 148
AsuHPI GGTGA 3 cut(s) 16, 91, 160
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 483
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 3 cut(s) 347, 763, 857
BccI CCATC 2 cut(s) 93, 199
BceAI ACGGC 2 cut(s) 27, 105
BciVI GTATCC 1 cut(s) 703
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 2 cut(s) 242, 800
BfmI CTRYAG 1 cut(s) 749
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 4 cut(s) 336, 702, 752, 846
BlpI GCTNAGC 1 cut(s) 684
BlsI GCNGC 4 cut(s) 337, 703, 753, 847
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 4 cut(s) 189, 294, 582, 594
BmsI GCATC 2 cut(s) 107, 335
Bpu1102I GCTNAGC 1 cut(s) 684
BsaJI CCNNGG 4 cut(s) 144, 177, 678, 837
BsaXI ACNNNNNCTCC 7 cut(s) 125, 155, 239, 269, 381, 411, 863
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 777
BseDI CCNNGG 4 cut(s) 144, 177, 678, 837
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 2 cut(s) 186, 675
BseRI GAGGAG 2 cut(s) 149, 260
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 3 cut(s) 347, 763, 857
Bsh1285I CGRYCG 1 cut(s) 418
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 483
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 1 cut(s) 403
BsnI GGCC 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 3 cut(s) 292, 448, 722
Bsp1720I GCTNAGC 1 cut(s) 684
Bsp19I CCATGG 1 cut(s) 837
BspACI CCGC 4 cut(s) 71, 155, 632, 701
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 187, 676
BspLI GGNNCC 4 cut(s) 189, 294, 582, 594
BspMAI CTGCAG 1 cut(s) 753
BspPI GGATC 4 cut(s) 287, 300, 443, 730
BspQI GCTCTTC 1 cut(s) 254
BspT107I GGYRCC 1 cut(s) 592
BsrBI CCGCTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 777
BssECI CCNNGG 4 cut(s) 144, 177, 678, 837
BssMI GATC 3 cut(s) 292, 448, 722
BssT1I CCWWGG 3 cut(s) 144, 678, 837
Bst4CI ACNGT 1 cut(s) 656
Bst6I CTCTTC 1 cut(s) 254
BstAPI GCANNNNNTGC 2 cut(s) 286, 332
BstC8I GCNNGC 2 cut(s) 444, 855
BstDEI CTNAG 2 cut(s) 195, 684
BstDSI CCRYGG 1 cut(s) 837
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 3 cut(s) 295, 451, 725
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 3 cut(s) 292, 448, 722
BstMCI CGRYCG 1 cut(s) 418
BstMWI GCNNNNNNNGC 4 cut(s) 286, 332, 487, 854
BstSFI CTRYAG 1 cut(s) 749
BstSLI GKGCMC 1 cut(s) 483
BstV1I GCAGC 3 cut(s) 347, 763, 857
BstX2I RGATCY 2 cut(s) 292, 448
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 292, 448
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 837
BtsCI GGATG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 873
Cac8I GCNNGC 2 cut(s) 444, 855
Cfr13I GGNCC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 9, 454
CviAII CATG 1 cut(s) 838
CviQI GTAC 2 cut(s) 9, 454
DdeI CTNAG 2 cut(s) 195, 684
DpnI GATC 3 cut(s) 294, 450, 724
DpnII GATC 3 cut(s) 292, 448, 722
DraI TTTAAA 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 254
EarI CTCTTC 1 cut(s) 254
EciI GGCGGA 1 cut(s) 647
Eco130I CCWWGG 3 cut(s) 144, 678, 837
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoT14I CCWWGG 3 cut(s) 144, 678, 837
EcoT22I ATGCAT 1 cut(s) 795
ErhI CCWWGG 3 cut(s) 144, 678, 837
FaeI CATG 1 cut(s) 841
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 1 cut(s) 837
FauI CCCGC 2 cut(s) 78, 162
Fnu4HI GCNGC 4 cut(s) 336, 702, 752, 846
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 336, 702, 752, 846
FspBI CTAG 2 cut(s) 242, 800
GluI GCNGC 4 cut(s) 336, 702, 752, 846
HaeIII GGCC 1 cut(s) 14
Hin1II CATG 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 5 cut(s) 60, 248, 311, 411, 604
HphI GGTGA 3 cut(s) 16, 91, 160
Hpy166II GTNNAC 2 cut(s) 481, 652
Hpy188I TCNGA 5 cut(s) 65, 196, 624, 736, 744
Hpy188III TCNNGA 2 cut(s) 48, 720
Hpy8I GTNNAC 2 cut(s) 481, 652
HpyAV CCTTC 5 cut(s) 25, 47, 54, 175, 684
HpyCH4III ACNGT 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 286, 332, 487, 854
HpyF3I CTNAG 2 cut(s) 195, 684
Hsp92II CATG 1 cut(s) 841
Kzo9I GATC 3 cut(s) 292, 448, 722
LguI GCTCTTC 1 cut(s) 254
LmnI GCTCC 4 cut(s) 162, 389, 586, 775
LpnPI CCDG 7 cut(s) 43, 170, 275, 352, 507, 705, 726
Lsp1109I GCAGC 3 cut(s) 347, 763, 857
LweI GCATC 2 cut(s) 107, 335
MaeI CTAG 2 cut(s) 242, 800
MalI GATC 3 cut(s) 294, 450, 724
MbiI CCGCTC 1 cut(s) 157
MboI GATC 3 cut(s) 292, 448, 722
MboII GAAGA 2 cut(s) 271, 332
MflI RGATCY 2 cut(s) 292, 448
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 4 cut(s) 434, 609, 761, 815
MlyI GAGTC 3 cut(s) 54, 257, 598
MnlI CCTC 9 cut(s) 92, 151, 161, 170, 172, 238, 401, 532, 583
Mph1103I ATGCAT 1 cut(s) 795
MseI TTAA 3 cut(s) 512, 554, 764
MslI CAYNNNNRTG 3 cut(s) 196, 216, 476
MwoI GCNNNNNNNGC 4 cut(s) 286, 332, 487, 854
NcoI CCATGG 1 cut(s) 837
NdeII GATC 3 cut(s) 292, 448, 722
NlaIII CATG 1 cut(s) 841
NlaIV GGNNCC 4 cut(s) 189, 294, 582, 594
NsiI ATGCAT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 254
PfeI GAWTC 2 cut(s) 311, 411
PkrI GCNGC 4 cut(s) 337, 703, 753, 847
PleI GAGTC 3 cut(s) 54, 256, 598
PpsI GAGTC 3 cut(s) 54, 256, 598
PspN4I GGNNCC 4 cut(s) 189, 294, 582, 594
PspPI GGNCC 1 cut(s) 148
PstI CTGCAG 1 cut(s) 753
PsuI RGATCY 2 cut(s) 292, 448
RsaI GTAC 2 cut(s) 10, 455
RsaNI GTAC 2 cut(s) 9, 454
RseI CAYNNNNRTG 3 cut(s) 196, 216, 476
SapI GCTCTTC 1 cut(s) 254
SaqAI TTAA 3 cut(s) 512, 554, 764
SatI GCNGC 4 cut(s) 336, 702, 752, 846
Sau3AI GATC 3 cut(s) 292, 448, 722
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 3 cut(s) 54, 257, 598
SduI GDGCHC 1 cut(s) 483
SfaNI GCATC 2 cut(s) 107, 335
SfcI CTRYAG 1 cut(s) 749
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 3 cut(s) 196, 216, 476
Sse9I AATT 4 cut(s) 434, 609, 761, 815
SsiI CCGC 4 cut(s) 71, 155, 632, 701
SspMI CTAG 2 cut(s) 242, 800
StyI CCWWGG 3 cut(s) 144, 678, 837
TaaI ACNGT 1 cut(s) 656
TaqI TCGA 1 cut(s) 257
TaqII GACCGA 3 cut(s) 67, 191, 404
TasI AATT 4 cut(s) 434, 609, 761, 815
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 2 cut(s) 311, 411
Tru1I TTAA 3 cut(s) 512, 554, 764
Tru9I TTAA 3 cut(s) 512, 554, 764
TscAI CASTG 1 cut(s) 873
TseI GCWGC 3 cut(s) 335, 751, 845
TspDTI ATGAA 5 cut(s) 200, 234, 303, 675, 798
TspGWI ACGGA 2 cut(s) 83, 441
TspRI CASTG 1 cut(s) 873
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 717
XapI RAATTY 1 cut(s) 434
XspI CTAG 2 cut(s) 242, 800
Zsp2I ATGCAT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.