RchiOBHm_Chr7g0227131

ATP-dependent protease La (LON) substrate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003413
Physical Location & Seq
Forward (+)
50491378 .. 50496041
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20341

Sequence Viewer

Length: 900 bp
ATGAATTGTACGGCCTTCAATCTCACCACCACCAGCCCCTTTCTCCCTGCAAGGACGACCCGACTCCGAAACCCGCACCCGACCCTCCACCGTTTCCTCCATTTCGGATTCACCCGCCCGAAGCAGAAACGATGCCGTTTCTCGCCGCCAAACGCCACCTCCTTGGAGCTCCCGCTGCTCCCCTTCACCTTGACCGAGGTTCTGGTTCCAACTGAGAGTAAAACACTGCATTTATATGAAGCCAGATATCTTGCTCTTCTCGAGGAGTCATTGTTGAGGAACAAGAAGTTCTTCGTGCATTTTGTGCTGGATCCTATCATTATTGATGATTCATTGGAAGAAGCATCTTTTGCAGCTAGAAATGGTTGTTTGGTATTTATAGAAAATGTTGAGAGATTAGAAGTTGGAGCATTAGTCTCTATAAGAGGAATTGGTCGTGTAAAGATTATCAAATTTCTGCAGGCAGATCCTTACTTGAAAGGTGTTGTCATACCCGTGCAGGACAGGGTACCTGATAGTGTAAGCAGATTAAGCCCCAAAGTTTTTCAAGTAAAAGAGGCTTTGTGTAGTTTAAATAGTTTGGAAATCAAACTAAAGGCTCCAAAGGAGGCACCTTTGCAGACTCAAATTGCCAACTCTCTGATGTGGACGGAAAAGGAACTACAAGTGGACTGTGATGAAGCTTTCTTTCCTTCCTTGGCTGAGCGTGTATCCTTTGCAGCACTTCAACCTGTATCAGGATCAAGTCAATCGGAACTTCTGAAGCTGCAGCAAGAGAAATTAAAGGCAATGGAGCTGAAAGACACAATGCATAGGCTAGATAATTCATTACAGTTTGTCAAAGATAATATCTCCATGGTTGCAGCCAAGCTTGCAATACAATCAGTAGAGATGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

299

Amino Acids

33.79

Weight (kDa)

8.9

Isoelectric Point (pI)

48.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 57 - 274 1.6e-10 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 508
AccB1I GGYRCC 2 cut(s) 508, 610
AciI CCGC 4 cut(s) 74, 115, 146, 173
AclWI GGATC 4 cut(s) 305, 318, 461, 748
AcsI RAATTY 1 cut(s) 452
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 2 cut(s) 10, 510
AfiI CCNNNNNNNGG 1 cut(s) 737
AgsI TTSAA 4 cut(s) 19, 478, 548, 728
AluBI AGCT 6 cut(s) 169, 356, 683, 766, 796, 871
AluI AGCT 6 cut(s) 169, 356, 683, 766, 796, 871
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 4 cut(s) 305, 318, 461, 748
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 6 cut(s) 175, 353, 719, 766, 769, 863
ApoI RAATTY 1 cut(s) 452
Asp700I GAANNNNTTC 1 cut(s) 290
Asp718I GGTACC 1 cut(s) 508
AsuHPI GGTGA 3 cut(s) 16, 103, 178
AvaI CYCGRG 1 cut(s) 260
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 2 cut(s) 508, 610
BanII GRGCYC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 6 cut(s) 162, 365, 731, 753, 781, 875
BceAI ACGGC 2 cut(s) 27, 120
BciVI GTATCC 1 cut(s) 721
BcoDI GTCTC 1 cut(s) 421
BfaI CTAG 2 cut(s) 357, 818
BfmI CTRYAG 2 cut(s) 458, 767
BfuI GTATCC 1 cut(s) 721
BisI GCNGC 7 cut(s) 146, 176, 354, 720, 767, 770, 864
BlpI GCTNAGC 1 cut(s) 702
BlsI GCNGC 7 cut(s) 147, 177, 355, 721, 768, 771, 865
BmeT110I CYCGRG 1 cut(s) 260
BmiI GGNNCC 5 cut(s) 207, 312, 510, 600, 612
BmsI GCATC 2 cut(s) 122, 353
Bpu1102I GCTNAGC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 162, 195, 696, 855
BsaXI ACNNNNNCTCC 6 cut(s) 143, 173, 257, 287, 399, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 737
Bse3DI GCAATG 1 cut(s) 795
BseDI CCNNGG 4 cut(s) 162, 195, 696, 855
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 2 cut(s) 204, 693
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 6 cut(s) 162, 365, 731, 753, 781, 875
BsgI GTGCAG 1 cut(s) 518
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 2 cut(s) 508, 610
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 421
BsnI GGCC 1 cut(s) 14
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 3 cut(s) 310, 466, 740
Bsp1720I GCTNAGC 1 cut(s) 702
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 4 cut(s) 74, 115, 146, 173
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 2 cut(s) 205, 694
BspLI GGNNCC 5 cut(s) 207, 312, 510, 600, 612
BspMAI CTGCAG 2 cut(s) 462, 771
BspPI GGATC 4 cut(s) 305, 318, 461, 748
BspQI GCTCTTC 1 cut(s) 261
BspT107I GGYRCC 2 cut(s) 508, 610
BsrDI GCAATG 1 cut(s) 795
BssECI CCNNGG 4 cut(s) 162, 195, 696, 855
BssMI GATC 3 cut(s) 310, 466, 740
BssT1I CCWWGG 3 cut(s) 162, 696, 855
Bst4CI ACNGT 3 cut(s) 92, 674, 834
Bst6I CTCTTC 1 cut(s) 261
BstAPI GCANNNNNTGC 2 cut(s) 304, 350
BstC8I GCNNGC 2 cut(s) 462, 873
BstDEI CTNAG 2 cut(s) 213, 702
BstDSI CCRYGG 1 cut(s) 855
BstENI CCTNNNNNAGG 1 cut(s) 735
BstKTI GATC 3 cut(s) 313, 469, 743
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 3 cut(s) 310, 466, 740
BstMWI GCNNNNNNNGC 5 cut(s) 175, 304, 350, 531, 872
BstSFI CTRYAG 2 cut(s) 458, 767
BstV1I GCAGC 6 cut(s) 162, 365, 731, 753, 781, 875
BstX2I RGATCY 2 cut(s) 310, 466
BstXI CCANNNNNNTGG 1 cut(s) 163
BstYI RGATCY 2 cut(s) 310, 466
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 855
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 462, 873
Csp6I GTAC 2 cut(s) 9, 509
CviAII CATG 1 cut(s) 856
CviQI GTAC 2 cut(s) 9, 509
DdeI CTNAG 2 cut(s) 213, 702
DpnI GATC 3 cut(s) 312, 468, 742
DpnII GATC 3 cut(s) 310, 466, 740
DraI TTTAAA 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 3 cut(s) 162, 696, 855
Eco24I GRGCYC 1 cut(s) 171
Eco32I GATATC 1 cut(s) 248
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 782
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 169
EcoNI CCTNNNNNAGG 1 cut(s) 735
EcoRV GATATC 1 cut(s) 248
EcoT14I CCWWGG 3 cut(s) 162, 696, 855
EcoT22I ATGCAT 1 cut(s) 813
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 162, 696, 855
FaeI CATG 1 cut(s) 859
FaiI YATR 7 cut(s) 235, 237, 380, 422, 491, 813, 857
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 1 cut(s) 855
FauI CCCGC 3 cut(s) 81, 122, 180
Fnu4HI GCNGC 7 cut(s) 146, 176, 354, 720, 767, 770, 864
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 7 cut(s) 146, 176, 354, 720, 767, 770, 864
FspBI CTAG 2 cut(s) 357, 818
GluI GCNGC 7 cut(s) 146, 176, 354, 720, 767, 770, 864
HaeIII GGCC 1 cut(s) 14
Hin1II CATG 1 cut(s) 859
HindIII AAGCTT 2 cut(s) 681, 869
HinfI GANTC 5 cut(s) 63, 108, 266, 329, 622
HphI GGTGA 3 cut(s) 16, 103, 178
Hpy166II GTNNAC 2 cut(s) 648, 670
Hpy188I TCNGA 5 cut(s) 68, 107, 642, 754, 762
Hpy188III TCNNGA 2 cut(s) 260, 738
Hpy8I GTNNAC 2 cut(s) 648, 670
HpyAV CCTTC 3 cut(s) 25, 193, 702
HpyCH4III ACNGT 3 cut(s) 92, 674, 834
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 304, 350, 531, 872
HpyF3I CTNAG 2 cut(s) 213, 702
Hsp92II CATG 1 cut(s) 859
KpnI GGTACC 1 cut(s) 512
Kzo9I GATC 3 cut(s) 310, 466, 740
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 6 cut(s) 166, 174, 183, 407, 604, 793
Lsp1109I GCAGC 6 cut(s) 162, 365, 731, 753, 781, 875
LweI GCATC 2 cut(s) 122, 353
MaeI CTAG 2 cut(s) 357, 818
MalI GATC 3 cut(s) 312, 468, 742
MboI GATC 3 cut(s) 310, 466, 740
MboII GAAGA 3 cut(s) 248, 283, 350
MflI RGATCY 2 cut(s) 310, 466
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 6 cut(s) 4, 429, 452, 627, 779, 823
MlyI GAGTC 3 cut(s) 57, 275, 616
MmeI TCCRAC 2 cut(s) 233, 385
MnlI CCTC 9 cut(s) 95, 107, 169, 190, 256, 270, 419, 550, 601
Mph1103I ATGCAT 1 cut(s) 813
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 3 cut(s) 530, 572, 782
MslI CAYNNNNRTG 2 cut(s) 234, 494
MspA1I CMGCKG 1 cut(s) 175
MwoI GCNNNNNNNGC 5 cut(s) 175, 304, 350, 531, 872
NcoI CCATGG 1 cut(s) 855
NdeII GATC 3 cut(s) 310, 466, 740
NlaIII CATG 1 cut(s) 859
NlaIV GGNNCC 5 cut(s) 207, 312, 510, 600, 612
NsiI ATGCAT 1 cut(s) 813
PaeR7I CTCGAG 1 cut(s) 260
PciSI GCTCTTC 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 2 cut(s) 108, 329
PkrI GCNGC 7 cut(s) 147, 177, 355, 721, 768, 771, 865
PleI GAGTC 3 cut(s) 57, 274, 616
PpsI GAGTC 3 cut(s) 57, 274, 616
Psp124BI GAGCTC 1 cut(s) 171
PspN4I GGNNCC 5 cut(s) 207, 312, 510, 600, 612
PstI CTGCAG 2 cut(s) 462, 771
PsuI RGATCY 2 cut(s) 310, 466
RsaI GTAC 2 cut(s) 10, 510
RsaNI GTAC 2 cut(s) 9, 509
RseI CAYNNNNRTG 2 cut(s) 234, 494
SacI GAGCTC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 530, 572, 782
SatI GCNGC 7 cut(s) 146, 176, 354, 720, 767, 770, 864
Sau3AI GATC 3 cut(s) 310, 466, 740
SchI GAGTC 3 cut(s) 57, 275, 616
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 2 cut(s) 122, 353
SfcI CTRYAG 2 cut(s) 458, 767
Sfr274I CTCGAG 1 cut(s) 260
SlaI CTCGAG 1 cut(s) 260
SmiMI CAYNNNNRTG 2 cut(s) 234, 494
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 6 cut(s) 4, 429, 452, 627, 779, 823
SsiI CCGC 4 cut(s) 74, 115, 146, 173
SspMI CTAG 2 cut(s) 357, 818
SstI GAGCTC 1 cut(s) 171
StyI CCWWGG 3 cut(s) 162, 696, 855
TaaI ACNGT 3 cut(s) 92, 674, 834
TaqI TCGA 1 cut(s) 261
TaqII GACCGA 1 cut(s) 209
TasI AATT 6 cut(s) 4, 429, 452, 627, 779, 823
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 108, 329
Tru1I TTAA 3 cut(s) 530, 572, 782
Tru9I TTAA 3 cut(s) 530, 572, 782
TscAI CASTG 1 cut(s) 231
TseI GCWGC 6 cut(s) 175, 353, 719, 766, 769, 863
TspDTI ATGAA 5 cut(s) 17, 252, 321, 693, 816
TspGWI ACGGA 1 cut(s) 665
TspRI CASTG 1 cut(s) 231
XagI CCTNNNNNAGG 1 cut(s) 735
XapI RAATTY 1 cut(s) 452
XhoI CTCGAG 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 2 cut(s) 357, 818
Zsp2I ATGCAT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.