MD14G1036300.v1.1

ATP-dependent protease La (LON) substrate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003413
Physical Location & Seq
Forward (+)
3270052 .. 3282731
12680 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1036300.v1.1.491

Sequence Viewer

Length: 927 bp
ATGAATTGTACGGCCTTCAAATTCAATCCCACCACTCCGTTTGTCCGGGACAAGCACGCTCAGTTTATACCAGCGACACGAATCCGAAACCCGCACCGGAATGTCTATAGTTCCGTTGGTTTCAGTTTTACCAGGAAGAAACTGAAACGATGCCGCCACTTTGCTCCTCCAAACGCCACCCCCTTGGTGGAATTGGAGCTCCCGCTCCTTCCCTTTCCCTTGAACGAGGTTCTAGTTCCCTCTGAGAGTAAGGCATTGCACTTATATGAAGCAAGGTATCTAGCTCTACTGGAGGAGTCATTGACAAGGAAGAAGCTTTTTGTGCATTTTGTGTTGGATCCTATAGTCATTGACAATTCATCAGGAGAAGCATCCTTTGCAGCCAGAAATGGCTGCTTGGTTTTCATAGAAAATGTTGAGCGTTTAGAAGTTGGAGCACTAGTCTCTATTAGAGGAATTGGTCGTGTAAAAATTGTGAAATTTGTGCAGGCAGAACCTTACTTGAAAGGTGTTGTCATACCTGTGCACGACAGGGTTCCTGAAAGCGAGAGTGGATTAGACCCAAAAGTTATGCAAGTAAAGGAAGCTCTATATAGTTTAAATAGCTTGGAAATCAAGCTAAAGGCCCCCAAGGAGGCACCGTTGCAGACTCGAATTGCCAATTCTCTAATGTGGAGTGAAAAGGAACTAGTCTTAGATTGTGATGAAGCTTTCATTCCTTCCTTGCCTGAGCGTGTTTCCTTTGCAGCACTTCAACCGGTATCAGGATCAGGTGAATCGGAACTTGCGAAGCTACAGCTAGAGAAGTTAAGGGCAATGGAGCTCAAAGACACCGTCCAGAGGCTAGATAATTCCCTGGAATTTGTTAAGGATAATATCTCCATGGTTGCAGCCAAGCTCGCTATACAATCAGTGGAGATGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

309

Amino Acids

34.56

Weight (kDa)

8.57

Isoelectric Point (pI)

33.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 67 - 198 1.4e-11 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 637
AccBSI CCGCTC 1 cut(s) 205
AciI CCGC 3 cut(s) 92, 154, 203
AclWI GGATC 3 cut(s) 332, 345, 775
AcsI RAATTY 3 cut(s) 20, 479, 860
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 4 cut(s) 187, 634, 764, 840
AgeI ACCGGT 1 cut(s) 757
AgsI TTSAA 5 cut(s) 19, 25, 223, 505, 755
AhlI ACTAGT 2 cut(s) 439, 688
AjnI CCWGG 2 cut(s) 131, 855
Alw21I GWGCWC 4 cut(s) 201, 439, 528, 825
Alw26I GTCTC 1 cut(s) 448
Alw44I GTGCAC 1 cut(s) 524
AlwI GGATC 3 cut(s) 332, 345, 775
AoxI GGCC 2 cut(s) 12, 624
ApaLI GTGCAC 1 cut(s) 524
ApeKI GCWGC 4 cut(s) 380, 393, 746, 890
ApoI RAATTY 3 cut(s) 20, 479, 860
ArsI GACNNNNNNTTYG 2 cut(s) 819, 851
AsiGI ACCGGT 1 cut(s) 757
AspS9I GGNCC 1 cut(s) 625
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 785
BaeGI GKGCMC 1 cut(s) 528
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 637
BanII GRGCYC 2 cut(s) 201, 825
Bbv12I GWGCWC 4 cut(s) 201, 439, 528, 825
BbvI GCAGC 4 cut(s) 380, 392, 758, 902
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 133, 857
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 448
BcuI ACTAGT 2 cut(s) 439, 688
BfaI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
BfmI CTRYAG 3 cut(s) 106, 342, 794
BisI GCNGC 5 cut(s) 154, 381, 394, 747, 891
BlsI GCNGC 5 cut(s) 155, 382, 395, 748, 892
Bme1390I CCNGG 3 cut(s) 47, 133, 857
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 4 cut(s) 339, 537, 627, 639
BmrFI CCNGG 3 cut(s) 47, 133, 857
BmsI GCATC 3 cut(s) 140, 380, 909
BpmI CTGGAG 1 cut(s) 311
Bpu10I CCTNAGC 1 cut(s) 729
BpuMI CCSGG 1 cut(s) 47
BsaJI CCNNGG 4 cut(s) 183, 630, 855, 882
BsaWI WCCGGW 2 cut(s) 96, 757
BsaXI ACNNNNNCTCC 5 cut(s) 426, 456, 626, 656, 908
Bsc4I CCNNNNNNNGG 4 cut(s) 187, 634, 764, 840
Bse118I RCCGGY 1 cut(s) 757
Bse1I ACTGG 1 cut(s) 294
Bse3DI GCAATG 2 cut(s) 254, 822
BseBI CCWGG 2 cut(s) 133, 857
BseDI CCNNGG 4 cut(s) 183, 630, 855, 882
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 4 cut(s) 187, 634, 764, 840
BseMI GCAATG 2 cut(s) 254, 822
BseMII CTCAG 3 cut(s) 74, 234, 720
BseNI ACTGG 1 cut(s) 294
BseRI GAGGAG 2 cut(s) 156, 308
BseSI GKGCMC 1 cut(s) 528
BseXI GCAGC 4 cut(s) 380, 392, 758, 902
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 2 cut(s) 14, 626
BshNI GGYRCC 1 cut(s) 637
BshTI ACCGGT 1 cut(s) 757
BsiHKAI GWGCWC 4 cut(s) 201, 439, 528, 825
BsiSI CCGG 3 cut(s) 46, 97, 758
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 4 cut(s) 187, 634, 764, 840
BsmAI GTCTC 1 cut(s) 448
BsmFI GGGAC 1 cut(s) 62
BsnI GGCC 2 cut(s) 14, 626
Bsp1286I GDGCHC 4 cut(s) 201, 439, 528, 825
Bsp143I GATC 2 cut(s) 337, 767
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 3 cut(s) 92, 154, 203
BspANI GGCC 2 cut(s) 14, 626
BspCNI CTCAG 3 cut(s) 73, 235, 721
BspLI GGNNCC 4 cut(s) 339, 537, 627, 639
BspPI GGATC 3 cut(s) 332, 345, 775
BspT107I GGYRCC 1 cut(s) 637
BsrBI CCGCTC 1 cut(s) 205
BsrDI GCAATG 2 cut(s) 254, 822
BsrFI RCCGGY 1 cut(s) 757
BsrI ACTGG 1 cut(s) 294
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 4 cut(s) 183, 630, 855, 882
BssMI GATC 2 cut(s) 337, 767
BssT1I CCWWGG 3 cut(s) 183, 630, 882
Bst2UI CCWGG 2 cut(s) 133, 857
Bst4CI ACNGT 2 cut(s) 642, 835
BstAPI GCANNNNNTGC 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 57, 489, 900
BstDEI CTNAG 4 cut(s) 60, 243, 694, 729
BstDSI CCRYGG 1 cut(s) 882
BstF5I GGATG 1 cut(s) 371
BstKTI GATC 2 cut(s) 340, 770
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 2 cut(s) 337, 767
BstMWI GCNNNNNNNGC 3 cut(s) 322, 377, 899
BstNI CCWGG 2 cut(s) 133, 857
BstSCI CCNGG 3 cut(s) 45, 131, 855
BstSFI CTRYAG 3 cut(s) 106, 342, 794
BstSLI GKGCMC 1 cut(s) 528
BstV1I GCAGC 4 cut(s) 380, 392, 758, 902
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 2 cut(s) 14, 626
BtgI CCRYGG 1 cut(s) 882
BtsCI GGATG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 918
Cac8I GCNNGC 3 cut(s) 57, 489, 900
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 625
Csp6I GTAC 1 cut(s) 9
CspAI ACCGGT 1 cut(s) 757
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviAII CATG 1 cut(s) 883
CviQI GTAC 1 cut(s) 9
DdeI CTNAG 4 cut(s) 60, 243, 694, 729
DpnI GATC 2 cut(s) 339, 769
DpnII GATC 2 cut(s) 337, 767
DraI TTTAAA 1 cut(s) 600
Ecl136II GAGCTC 2 cut(s) 199, 823
Eco130I CCWWGG 3 cut(s) 183, 630, 882
Eco24I GRGCYC 2 cut(s) 201, 825
Eco53kI GAGCTC 2 cut(s) 199, 823
EcoICRI GAGCTC 2 cut(s) 199, 823
EcoO109I RGGNCCY 1 cut(s) 625
EcoRII CCWGG 2 cut(s) 131, 855
EcoT14I CCWWGG 3 cut(s) 183, 630, 882
EcoT38I GRGCYC 2 cut(s) 201, 825
ErhI CCWWGG 3 cut(s) 183, 630, 882
FaeI CATG 1 cut(s) 886
FaqI GGGAC 1 cut(s) 62
FatI CATG 1 cut(s) 882
FauI CCCGC 2 cut(s) 99, 210
Fnu4HI GCNGC 5 cut(s) 154, 381, 394, 747, 891
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 201, 825
Fsp4HI GCNGC 5 cut(s) 154, 381, 394, 747, 891
FspBI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
GluI GCNGC 5 cut(s) 154, 381, 394, 747, 891
GsuI CTGGAG 1 cut(s) 311
HaeIII GGCC 2 cut(s) 14, 626
HapII CCGG 3 cut(s) 46, 97, 758
Hin1II CATG 1 cut(s) 886
HindIII AAGCTT 2 cut(s) 314, 708
HinfI GANTC 4 cut(s) 81, 296, 649, 776
HpaII CCGG 3 cut(s) 46, 97, 758
HphI GGTGA 1 cut(s) 785
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 3 cut(s) 86, 244, 781
Hpy188III TCNNGA 4 cut(s) 363, 539, 765, 838
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 3 cut(s) 25, 218, 729
HpyCH4III ACNGT 2 cut(s) 642, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 377, 899
HpyF3I CTNAG 4 cut(s) 60, 243, 694, 729
Hsp92II CATG 1 cut(s) 886
Kzo9I GATC 2 cut(s) 337, 767
LmnI GCTCC 6 cut(s) 169, 196, 204, 210, 434, 820
Lsp1109I GCAGC 4 cut(s) 380, 392, 758, 902
LweI GCATC 3 cut(s) 140, 380, 909
MaeI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
MalI GATC 2 cut(s) 339, 769
MbiI CCGCTC 1 cut(s) 205
MboI GATC 2 cut(s) 337, 767
MboII GAAGA 2 cut(s) 148, 322
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 4 cut(s) 201, 439, 528, 825
MlyI GAGTC 2 cut(s) 305, 643
MmeI TCCRAC 2 cut(s) 315, 412
MnlI CCTC 7 cut(s) 177, 220, 250, 286, 446, 628, 834
MseI TTAA 3 cut(s) 599, 809, 867
MslI CAYNNNNRTG 3 cut(s) 99, 264, 521
MspI CCGG 3 cut(s) 46, 97, 758
MspR9I CCNGG 3 cut(s) 47, 133, 857
MvaI CCWGG 2 cut(s) 133, 857
MwoI GCNNNNNNNGC 3 cut(s) 322, 377, 899
NciI CCSGG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 882
NdeII GATC 2 cut(s) 337, 767
NlaIII CATG 1 cut(s) 886
NlaIV GGNNCC 4 cut(s) 339, 537, 627, 639
PcsI WCGNNNNNNNCGW 1 cut(s) 785
PfeI GAWTC 2 cut(s) 81, 776
PflFI GACNNNGTC 1 cut(s) 833
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 757
PkrI GCNGC 5 cut(s) 155, 382, 395, 748, 892
PleI GAGTC 2 cut(s) 304, 643
PpsI GAGTC 2 cut(s) 304, 643
Psp124BI GAGCTC 2 cut(s) 201, 825
Psp6I CCWGG 2 cut(s) 131, 855
PspGI CCWGG 2 cut(s) 131, 855
PspN4I GGNNCC 4 cut(s) 339, 537, 627, 639
PspPI GGNCC 1 cut(s) 625
PsuI RGATCY 1 cut(s) 337
PsyI GACNNNGTC 1 cut(s) 833
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 3 cut(s) 99, 264, 521
SacI GAGCTC 2 cut(s) 201, 825
SaqAI TTAA 3 cut(s) 599, 809, 867
SatI GCNGC 5 cut(s) 154, 381, 394, 747, 891
Sau3AI GATC 2 cut(s) 337, 767
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 2 cut(s) 305, 643
ScrFI CCNGG 3 cut(s) 47, 133, 857
SduI GDGCHC 4 cut(s) 201, 439, 528, 825
SfaNI GCATC 3 cut(s) 140, 380, 909
SfcI CTRYAG 3 cut(s) 106, 342, 794
SmiMI CAYNNNNRTG 3 cut(s) 99, 264, 521
SpeI ACTAGT 2 cut(s) 439, 688
SsiI CCGC 3 cut(s) 92, 154, 203
SspMI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
SstI GAGCTC 2 cut(s) 201, 825
StyD4I CCNGG 3 cut(s) 45, 131, 855
StyI CCWWGG 3 cut(s) 183, 630, 882
TaaI ACNGT 2 cut(s) 642, 835
TaqI TCGA 1 cut(s) 652
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 2 cut(s) 81, 776
Tru1I TTAA 3 cut(s) 599, 809, 867
Tru9I TTAA 3 cut(s) 599, 809, 867
TscAI CASTG 1 cut(s) 918
TseI GCWGC 4 cut(s) 380, 393, 746, 890
TspDTI ATGAA 6 cut(s) 17, 282, 348, 394, 703, 720
TspGWI ACGGA 2 cut(s) 27, 103
TspRI CASTG 1 cut(s) 918
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 524
XapI RAATTY 3 cut(s) 20, 479, 860
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 6 cut(s) 233, 281, 440, 689, 800, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.