FvH4_5g32940

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009914
Physical Location & Seq
Forward (+)
23782026 .. 23783127
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32940.t1

Sequence Viewer

Length: 903 bp
ATGAGGTCCAAACCTCAATGTGGCATCAATCTCAAGCTTATTGTCCTATTCTGCTCCTTTCTCCTCCTCTTCACCATCTTAATACTCCGATCAAACCTCTCATCTCCTCTAAAAACTCCACCATCATCATCCACCACGATCTCAGAAACCCACCTCTCAAATTCATCTGATCAACCTCAGCAAGTCCCAAAATGTCCATCGCTCGAAGTACCCTTCATCCCTACATGCACAAAAACCCCACCTTCCTTAGCCAACGCCCTCATCCACTACGCCACCACCAACATCACCCCGCAGCAAACCTTCAAGGAAATATCAGTATCAGCAAGGATCCTAGCAAAGAAGGCCCCCTGCAACTTCCTCGTCTTCGGCTTAGGCCTCGACAGCCTCATGTGGGCGGCTCTCAACCACGGCGGCCGTACCGTCTTCCTAGAAGAAGACCAGTCCTGGATCGACCAGATCAAAGAACGACTCCCCAACTTGGAATCCTACCACGTCGTGTACGACACCAAAGTGCATGAAGCCGAGAAGCTCTTCGAGCATGGCTCGAAGGAAGAGTGCAAGACAGTGAGTGATCCAAGGTTTTCCAAGTGCCCATTGGCGCTGAAGAACTTGCCGGGAGATGTGTACGACATGGAGTGGGATTTGATCATGGTGGATGCGCCTACAGGGTACTTTGATGGAGCTCCTGGTCGGATGAGTGCTATATACACGGCGGGGTTGATGGGGAGGAACAGAGAGGAGGGTGAGACTGATGTGTTTGTTCATGATGTGGATAGAGTGGTCGAGGATAAATTCTCCAAAGCGTTTCTTTGTGAAGGGTATTTGAGAGAACAAGAGGGAAGGATTAGGCATTTTAAGATTCCGAGTCACAGGGCTCGTCTGGGCAGAACCTTTTGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

301

Amino Acids

33.74

Weight (kDa)

6.75

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 102 - 285 3.4e-78 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 290, 395, 411, 713
AclWI GGATC 4 cut(s) 322, 335, 455, 566
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 160, 791
AcuI CTGAAG 1 cut(s) 623
AdeI CACNNNGTG 1 cut(s) 496
AfaI GTAC 5 cut(s) 210, 418, 500, 626, 671
AfiI CCNNNNNNNGG 3 cut(s) 20, 391, 478
AgsI TTSAA 1 cut(s) 304
AjiI CACGTC 1 cut(s) 493
AjnI CCWGG 2 cut(s) 443, 685
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 1 cut(s) 880
AluBI AGCT 3 cut(s) 37, 529, 683
AluI AGCT 3 cut(s) 37, 529, 683
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 4 cut(s) 322, 335, 455, 566
AoxI GGCC 3 cut(s) 342, 373, 412
ApeKI GCWGC 1 cut(s) 292
ApoI RAATTY 2 cut(s) 160, 791
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 2 cut(s) 601, 661
AspS9I GGNCC 2 cut(s) 6, 343
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 3 cut(s) 64, 277, 755
AvaII GGWCC 1 cut(s) 6
BaeGI GKGCMC 1 cut(s) 593
BamHI GGATCC 1 cut(s) 327
BanII GRGCYC 2 cut(s) 685, 877
BbsI GAAGAC 3 cut(s) 355, 415, 441
Bbv12I GWGCWC 1 cut(s) 685
BbvCI CCTCAGC 1 cut(s) 177
BbvI GCAGC 1 cut(s) 304
BccI CCATC 5 cut(s) 83, 130, 205, 671, 715
BceAI ACGGC 3 cut(s) 399, 424, 726
BcgI CGANNNNNNTGC 2 cut(s) 340, 374
BciT130I CCWGG 2 cut(s) 445, 687
BclI TGATCA 2 cut(s) 169, 645
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 1 cut(s) 740
BfaI CTAG 2 cut(s) 332, 428
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 602
BisI GCNGC 3 cut(s) 293, 396, 412
BlsI GCNGC 3 cut(s) 294, 397, 413
Bme1390I CCNGG 3 cut(s) 445, 615, 687
Bme18I GGWCC 1 cut(s) 6
BmgBI CACGTC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 6, 343
BmiI GGNNCC 2 cut(s) 329, 345
BmrFI CCNGG 3 cut(s) 445, 615, 687
BmsI GCATC 2 cut(s) 33, 646
BpiI GAAGAC 3 cut(s) 355, 415, 441
BplI GAGNNNNNCTC 2 cut(s) 527, 559
Bpu10I CCTNAGC 3 cut(s) 177, 247, 370
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 615
BsaJI CCNNGG 2 cut(s) 406, 575
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 391, 478
Bse1I ACTGG 1 cut(s) 439
BseBI CCWGG 2 cut(s) 445, 687
BseDI CCNNGG 2 cut(s) 406, 575
BseGI GGATG 5 cut(s) 128, 216, 261, 661, 699
BseLI CCNNNNNNNGG 3 cut(s) 20, 391, 478
BseMII CTCAG 2 cut(s) 156, 191
BseNI ACTGG 1 cut(s) 439
BseRI GAGGAG 4 cut(s) 53, 56, 96, 752
BseSI GKGCMC 1 cut(s) 593
BseX3I CGGCCG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 3 cut(s) 344, 375, 414
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 1 cut(s) 614
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 20, 391, 478
BsmAI GTCTC 1 cut(s) 740
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 3 cut(s) 344, 375, 414
Bsp1286I GDGCHC 3 cut(s) 593, 685, 877
Bsp143I GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
BspACI CCGC 4 cut(s) 290, 395, 411, 713
BspANI GGCC 3 cut(s) 344, 375, 414
BspCNI CTCAG 2 cut(s) 155, 190
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 2 cut(s) 329, 345
BspPI GGATC 4 cut(s) 322, 335, 455, 566
BspQI GCTCTTC 1 cut(s) 536
BsrI ACTGG 1 cut(s) 439
BssECI CCNNGG 2 cut(s) 406, 575
BssMI GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
BssT1I CCWWGG 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 445, 687
Bst4CI ACNGT 2 cut(s) 421, 565
Bst6I CTCTTC 3 cut(s) 74, 536, 546
BstDEI CTNAG 4 cut(s) 142, 177, 247, 370
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 5 cut(s) 128, 216, 261, 661, 699
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 2 cut(s) 601, 661
BstKTI GATC 8 cut(s) 92, 141, 172, 330, 450, 459, 574, 648
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 341, 381, 535
BstNI CCWGG 2 cut(s) 445, 687
BstNSI RCATGY 1 cut(s) 228
BstSCI CCNGG 3 cut(s) 443, 613, 685
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 593
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 3 cut(s) 355, 415, 441
BstX2I RGATCY 1 cut(s) 327
BstYI RGATCY 1 cut(s) 327
BstZI CGGCCG 1 cut(s) 412
BsuRI GGCC 3 cut(s) 344, 375, 414
BtgI CCRYGG 1 cut(s) 406
BtgZI GCGATG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 493
BtsCI GGATG 5 cut(s) 128, 216, 261, 661, 699
BtsIMutI CAGTG 1 cut(s) 570
CciI TCATGA 1 cut(s) 763
CfoI GCGC 2 cut(s) 601, 661
Cfr13I GGNCC 2 cut(s) 6, 343
Csp6I GTAC 5 cut(s) 209, 417, 499, 625, 670
CviAII CATG 8 cut(s) 225, 388, 515, 539, 631, 649, 764, 900
CviQI GTAC 5 cut(s) 209, 417, 499, 625, 670
DdeI CTNAG 4 cut(s) 142, 177, 247, 370
DpnI GATC 8 cut(s) 91, 140, 171, 329, 449, 458, 573, 647
DpnII GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
DraIII CACNNNGTG 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 412
EagI CGGCCG 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 74, 536, 546
EarI CTCTTC 3 cut(s) 74, 536, 546
Ecl136II GAGCTC 1 cut(s) 683
EclXI CGGCCG 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 575
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 2 cut(s) 685, 877
Eco47I GGWCC 1 cut(s) 6
Eco52I CGGCCG 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 683
EcoO109I RGGNCCY 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 443, 685
EcoT14I CCWWGG 1 cut(s) 575
EcoT38I GRGCYC 2 cut(s) 685, 877
ErhI CCWWGG 1 cut(s) 575
FaeI CATG 8 cut(s) 228, 391, 518, 542, 634, 652, 767, 903
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 170
FatI CATG 8 cut(s) 224, 387, 514, 538, 630, 648, 763, 899
FauI CCCGC 2 cut(s) 297, 706
FbaI TGATCA 2 cut(s) 169, 645
Fnu4HI GCNGC 3 cut(s) 293, 396, 412
FokI GGATG 5 cut(s) 115, 203, 248, 668, 706
FriOI GRGCYC 2 cut(s) 685, 877
Fsp4HI GCNGC 3 cut(s) 293, 396, 412
FspBI CTAG 2 cut(s) 332, 428
GlaI GCGC 2 cut(s) 600, 660
GluI GCNGC 3 cut(s) 293, 396, 412
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 3 cut(s) 344, 375, 414
HapII CCGG 1 cut(s) 614
HhaI GCGC 2 cut(s) 601, 661
Hin1II CATG 8 cut(s) 228, 391, 518, 542, 634, 652, 767, 903
Hin6I GCGC 2 cut(s) 599, 659
HinP1I GCGC 2 cut(s) 599, 659
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 4 cut(s) 468, 482, 859, 865
HpaII CCGG 1 cut(s) 614
HphI GGTGA 3 cut(s) 64, 277, 755
Hpy166II GTNNAC 2 cut(s) 499, 625
Hpy188I TCNGA 5 cut(s) 89, 145, 169, 693, 864
Hpy188III TCNNGA 1 cut(s) 764
Hpy8I GTNNAC 2 cut(s) 499, 625
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 7 cut(s) 223, 252, 310, 334, 541, 809, 834
HpyCH4III ACNGT 2 cut(s) 421, 565
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 4 cut(s) 228, 351, 514, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 381, 535
HpyF3I CTNAG 4 cut(s) 142, 177, 247, 370
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 8 cut(s) 228, 391, 518, 542, 634, 652, 767, 903
HspAI GCGC 2 cut(s) 599, 659
Ksp22I TGATCA 2 cut(s) 169, 645
Kzo9I GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
LguI GCTCTTC 1 cut(s) 536
LmnI GCTCC 3 cut(s) 59, 680, 688
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 2 cut(s) 33, 646
MaeI CTAG 2 cut(s) 332, 428
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 1 cut(s) 866
MalI GATC 8 cut(s) 91, 140, 171, 329, 449, 458, 573, 647
MboI GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
MboII GAAGA 8 cut(s) 61, 355, 415, 443, 446, 523, 563, 616
MflI RGATCY 1 cut(s) 327
MhlI GDGCHC 3 cut(s) 593, 685, 877
MluCI AATT 2 cut(s) 160, 791
MlyI GAGTC 2 cut(s) 462, 874
MmeI TCCRAC 1 cut(s) 671
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 2 cut(s) 80, 855
MslI CAYNNNNRTG 1 cut(s) 509
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 445, 615, 687
MvaI CCWGG 2 cut(s) 445, 687
MwoI GCNNNNNNNGC 3 cut(s) 341, 381, 535
NciI CCSGG 1 cut(s) 615
NdeII GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
NlaIII CATG 8 cut(s) 228, 391, 518, 542, 634, 652, 767, 903
NlaIV GGNNCC 2 cut(s) 329, 345
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 1 cut(s) 866
NspI RCATGY 1 cut(s) 228
OliI CACNNNNGTG 1 cut(s) 509
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 482, 859
PfoI TCCNGGA 1 cut(s) 443
PkrI GCNGC 3 cut(s) 294, 397, 413
PleI GAGTC 2 cut(s) 462, 873
PpsI GAGTC 2 cut(s) 462, 873
Psp124BI GAGCTC 1 cut(s) 685
Psp6I CCWGG 2 cut(s) 443, 685
PspGI CCWGG 2 cut(s) 443, 685
PspN4I GGNNCC 2 cut(s) 329, 345
PspPI GGNCC 2 cut(s) 6, 343
PsuI RGATCY 1 cut(s) 327
RsaI GTAC 5 cut(s) 210, 418, 500, 626, 671
RsaNI GTAC 5 cut(s) 209, 417, 499, 625, 670
RseI CAYNNNNRTG 1 cut(s) 509
SacI GAGCTC 1 cut(s) 685
SapI GCTCTTC 1 cut(s) 536
SaqAI TTAA 2 cut(s) 80, 855
SatI GCNGC 3 cut(s) 293, 396, 412
Sau3AI GATC 8 cut(s) 89, 138, 169, 327, 447, 456, 571, 645
Sau96I GGNCC 2 cut(s) 6, 343
SchI GAGTC 2 cut(s) 462, 874
ScrFI CCNGG 3 cut(s) 445, 615, 687
SduI GDGCHC 3 cut(s) 593, 685, 877
SfaNI GCATC 2 cut(s) 33, 646
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
Sse9I AATT 2 cut(s) 160, 791
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 4 cut(s) 290, 395, 411, 713
SspMI CTAG 2 cut(s) 332, 428
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 3 cut(s) 443, 613, 685
StyI CCWWGG 1 cut(s) 575
TaaI ACNGT 2 cut(s) 421, 565
TaiI ACGT 1 cut(s) 495
TaqI TCGA 6 cut(s) 204, 378, 450, 534, 545, 783
TasI AATT 2 cut(s) 160, 791
TauI GCSGC 2 cut(s) 398, 414
TfiI GAWTC 2 cut(s) 482, 859
Tru1I TTAA 2 cut(s) 80, 855
Tru9I TTAA 2 cut(s) 80, 855
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 866
TspDTI ATGAA 4 cut(s) 153, 205, 531, 752
TspRI CASTG 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 6
XapI RAATTY 2 cut(s) 160, 791
XceI RCATGY 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 2 cut(s) 332, 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.