MD15G1399700.v1.1

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009914
Physical Location & Seq
Forward (+)
50032547 .. 50033437
891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1399700.v1.1.491

Sequence Viewer

Length: 891 bp
ATGAGGTCCAACCCGCAATGTGGCATCAATCTCAAGGTCATCCTCCTCTGCTGCTTCTTCATCTTCCTCTTCACTATCTTCATTGTCAGATCAACCTTCTCATCGTCACCATTACCGTCACCGTTCACGGCCAATTCCTCACTATCCCATCTCATCCCAAACCCTAAACCCCAACAACAACAACCACCATGCGATCAACCCTTCACCCCAACATGCACCAAAATTCCACCTTCCCTCGCCCACGCCATCATCCACTACGCCACAACAAACGTCACCCCACAACAAACCATCAAAGAAGTCTCCGTCTCCGCCGCGATCCTTTCCAAAAAATCACCCTGCAACTTTCTAGTCTTCGGCCTCGGCCTCGACAGCCTCATGTGGACGGCTCTCAACCACGGCGGAAGAACCGTCTTCCTCGAAGAAGACAATTCATGGATCGACCAGATCAAACAGAAGCTGCCGGGCTTAGAGGCCTACCACGTGACGTACGACACCAAGGTCCACCAAGCGGACAAGCTCATGGAGATCGGAAGGAAGGAAGAGTGCAAAGCTGTTGGGGATCCGAGGTTTTCAAAGTGCGAGCTGGCGCTCAAGAACTTGCCGAGCGATGTGTACGATATCGAGTGGGATTTGATCATGGTGGATGCGCCGACGGGGTACTTCGACGGAGCTCCGGGAAGAATGGGCGCCATATACACGGCGGGGCTGATGGGGAGGAACAGGGAGGAGGGGGAAACCCATGTGTTTGTGCATGATGTGAATAGAGTGGTTGAGGACAGCTTCTCCAAGGCTTTTCTCTGTGAGGGGTACTTGACGGAGCAGGAGGGTCTGATCAGGCACTTCACTATTCCCAGTCACAGGGCTCGATTAGGTAGACCATTTTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

297

Amino Acids

33.09

Weight (kDa)

6.55

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 98 - 281 2.9e-78 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 497
AccB1I GGYRCC 1 cut(s) 686
AccI GTMKAC 1 cut(s) 874
AccII CGCG 1 cut(s) 314
AciI CCGC 6 cut(s) 14, 309, 312, 399, 509, 701
AclWI GGATC 4 cut(s) 310, 443, 554, 567
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 222
AcvI CACGTG 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 687
AfaI GTAC 4 cut(s) 488, 614, 659, 809
AfiI CCNNNNNNNGG 3 cut(s) 20, 508, 858
AgsI TTSAA 1 cut(s) 573
AluBI AGCT 6 cut(s) 457, 517, 551, 583, 671, 780
AluI AGCT 6 cut(s) 457, 517, 551, 583, 671, 780
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 2 cut(s) 304, 310
AlwI GGATC 4 cut(s) 310, 443, 554, 567
AlwNI CAGNNNCTG 1 cut(s) 457
AoxI GGCC 4 cut(s) 129, 355, 361, 471
ApeKI GCWGC 2 cut(s) 51, 457
ApoI RAATTY 1 cut(s) 222
AspLEI GCGC 3 cut(s) 589, 649, 689
AspS9I GGNCC 2 cut(s) 6, 499
AsuC2I CCSGG 2 cut(s) 462, 675
AsuHPI GGTGA 5 cut(s) 99, 111, 196, 265, 324
AvaII GGWCC 2 cut(s) 6, 499
BamHI GGATCC 1 cut(s) 559
BanI GGYRCC 1 cut(s) 686
BanII GRGCYC 2 cut(s) 673, 865
BbrPI CACGTG 1 cut(s) 481
BbsI GAAGAC 3 cut(s) 343, 403, 429
Bbv12I GWGCWC 1 cut(s) 673
BbvI GCAGC 2 cut(s) 38, 444
BccI CCATC 4 cut(s) 156, 254, 296, 703
BceAI ACGGC 4 cut(s) 144, 399, 412, 714
BclI TGATCA 2 cut(s) 633, 831
BcnI CCSGG 2 cut(s) 462, 675
BcoDI GTCTC 2 cut(s) 304, 310
BfaI CTAG 1 cut(s) 347
BfoI RGCGCY 2 cut(s) 590, 690
BisI GCNGC 3 cut(s) 52, 312, 458
BlsI GCNGC 3 cut(s) 53, 313, 459
Bme1390I CCNGG 2 cut(s) 462, 675
Bme18I GGWCC 2 cut(s) 6, 499
BmgT120I GGNCC 2 cut(s) 6, 499
BmiI GGNNCC 2 cut(s) 561, 688
BmrFI CCNGG 2 cut(s) 462, 675
BmrI ACTGGG 1 cut(s) 846
BmsI GCATC 2 cut(s) 33, 634
BmuI ACTGGG 1 cut(s) 846
BpiI GAAGAC 3 cut(s) 343, 403, 429
BpuEI CTTGAG 2 cut(s) 17, 575
BpuMI CCSGG 2 cut(s) 462, 675
BsaAI YACGTR 1 cut(s) 481
BsaHI GRCGYC 1 cut(s) 687
BsaJI CCNNGG 5 cut(s) 358, 394, 495, 563, 786
BsaXI ACNNNNNCTCC 2 cut(s) 767, 797
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 508, 858
Bse1I ACTGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 23
BseDI CCNNGG 5 cut(s) 358, 394, 495, 563, 786
BseGI GGATG 4 cut(s) 39, 153, 249, 649
BseLI CCNNNNNNNGG 3 cut(s) 20, 508, 858
BseMI GCAATG 1 cut(s) 23
BseNI ACTGG 1 cut(s) 852
BseRI GAGGAG 2 cut(s) 35, 740
BseXI GCAGC 2 cut(s) 38, 444
Bsh1236I CGCG 1 cut(s) 314
BshFI GGCC 4 cut(s) 131, 357, 363, 473
BshNI GGYRCC 1 cut(s) 686
BsiHKAI GWGCWC 1 cut(s) 673
BsiSI CCGG 2 cut(s) 461, 674
BsiWI CGTACG 1 cut(s) 486
BslI CCNNNNNNNGG 3 cut(s) 20, 508, 858
BsmAI GTCTC 2 cut(s) 304, 310
BsmBI CGTCTC 1 cut(s) 310
BsnI GGCC 4 cut(s) 131, 357, 363, 473
Bsp1286I GDGCHC 2 cut(s) 673, 865
Bsp143I GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
BspACI CCGC 6 cut(s) 14, 309, 312, 399, 509, 701
BspANI GGCC 4 cut(s) 131, 357, 363, 473
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 2 cut(s) 561, 688
BspPI GGATC 4 cut(s) 310, 443, 554, 567
BspT107I GGYRCC 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 23
BsrI ACTGG 1 cut(s) 852
BssECI CCNNGG 5 cut(s) 358, 394, 495, 563, 786
BssMI GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
BssNI GRCGYC 1 cut(s) 687
BssT1I CCWWGG 2 cut(s) 495, 786
Bst4CI ACNGT 3 cut(s) 117, 123, 409
Bst6I CTCTTC 2 cut(s) 74, 534
BstACI GRCGYC 1 cut(s) 687
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 581, 585
BstDEI CTNAG 2 cut(s) 466, 888
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 4 cut(s) 39, 153, 249, 649
BstFNI CGCG 1 cut(s) 314
BstH2I RGCGCY 2 cut(s) 590, 690
BstHHI GCGC 3 cut(s) 589, 649, 689
BstKTI GATC 9 cut(s) 92, 196, 318, 438, 447, 528, 562, 636, 834
BstMAI GTCTC 2 cut(s) 304, 310
BstMBI GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
BstMWI GCNNNNNNNGC 1 cut(s) 369
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 2 cut(s) 460, 673
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 2 cut(s) 38, 444
BstV2I GAAGAC 3 cut(s) 343, 403, 429
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 4 cut(s) 131, 357, 363, 473
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 4 cut(s) 39, 153, 249, 649
Cac8I GCNNGC 2 cut(s) 581, 585
CaiI CAGNNNCTG 1 cut(s) 457
CfoI GCGC 3 cut(s) 589, 649, 689
Cfr13I GGNCC 2 cut(s) 6, 499
Csp6I GTAC 4 cut(s) 487, 613, 658, 808
CviAII CATG 8 cut(s) 189, 213, 376, 432, 520, 637, 740, 752
CviQI GTAC 4 cut(s) 487, 613, 658, 808
DdeI CTNAG 2 cut(s) 466, 888
DinI GGCGCC 1 cut(s) 688
DpnI GATC 9 cut(s) 91, 195, 317, 437, 446, 527, 561, 635, 833
DpnII GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
DrdI GACNNNNNNGTC 1 cut(s) 497
DseDI GACNNNNNNGTC 1 cut(s) 497
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 74, 534
EarI CTCTTC 2 cut(s) 74, 534
EciI GGCGGA 2 cut(s) 298, 414
Ecl136II GAGCTC 1 cut(s) 671
Eco130I CCWWGG 2 cut(s) 495, 786
Eco147I AGGCCT 1 cut(s) 473
Eco24I GRGCYC 2 cut(s) 673, 865
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 2 cut(s) 6, 499
Eco53kI GAGCTC 1 cut(s) 671
Eco72I CACGTG 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 671
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 2 cut(s) 495, 786
EcoT38I GRGCYC 2 cut(s) 673, 865
EgeI GGCGCC 1 cut(s) 688
EheI GGCGCC 1 cut(s) 688
ErhI CCWWGG 2 cut(s) 495, 786
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 8 cut(s) 192, 216, 379, 435, 523, 640, 743, 755
FatI CATG 8 cut(s) 188, 212, 375, 431, 519, 636, 739, 751
FauI CCCGC 2 cut(s) 21, 694
FbaI TGATCA 2 cut(s) 633, 831
FblI GTMKAC 1 cut(s) 874
Fnu4HI GCNGC 3 cut(s) 52, 312, 458
FokI GGATG 4 cut(s) 26, 140, 236, 656
FriOI GRGCYC 2 cut(s) 673, 865
Fsp4HI GCNGC 3 cut(s) 52, 312, 458
FspBI CTAG 1 cut(s) 347
GlaI GCGC 3 cut(s) 588, 648, 688
GluI GCNGC 3 cut(s) 52, 312, 458
HaeII RGCGCY 2 cut(s) 590, 690
HaeIII GGCC 4 cut(s) 131, 357, 363, 473
HapII CCGG 2 cut(s) 461, 674
HhaI GCGC 3 cut(s) 589, 649, 689
Hin1I GRCGYC 1 cut(s) 687
Hin1II CATG 8 cut(s) 192, 216, 379, 435, 523, 640, 743, 755
Hin6I GCGC 3 cut(s) 587, 647, 687
HinP1I GCGC 3 cut(s) 587, 647, 687
HpaII CCGG 2 cut(s) 461, 674
HphI GGTGA 5 cut(s) 99, 111, 196, 265, 324
Hpy166II GTNNAC 5 cut(s) 126, 381, 502, 613, 875
Hpy188I TCNGA 4 cut(s) 89, 530, 564, 831
Hpy188III TCNNGA 1 cut(s) 592
Hpy8I GTNNAC 5 cut(s) 126, 381, 502, 613, 875
Hpy99I CGWCG 2 cut(s) 655, 668
HpyAV CCTTC 5 cut(s) 106, 211, 240, 525, 529
HpyCH4III ACNGT 3 cut(s) 117, 123, 409
HpyCH4IV ACGT 3 cut(s) 270, 480, 485
HpyCH4V TGCA 4 cut(s) 216, 339, 546, 751
HpyF10VI GCNNNNNNNGC 1 cut(s) 369
HpyF3I CTNAG 2 cut(s) 466, 888
HpySE526I ACGT 3 cut(s) 270, 480, 485
Hsp92I GRCGYC 1 cut(s) 687
Hsp92II CATG 8 cut(s) 192, 216, 379, 435, 523, 640, 743, 755
HspAI GCGC 3 cut(s) 587, 647, 687
KasI GGCGCC 1 cut(s) 686
Ksp22I TGATCA 2 cut(s) 633, 831
Kzo9I GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
LmnI GCTCC 3 cut(s) 668, 676, 817
Lsp1109I GCAGC 2 cut(s) 38, 444
LweI GCATC 2 cut(s) 33, 634
MaeI CTAG 1 cut(s) 347
MaeII ACGT 3 cut(s) 270, 480, 485
MaeIII GTNAC 5 cut(s) 105, 117, 271, 481, 854
MalI GATC 9 cut(s) 91, 195, 317, 437, 446, 527, 561, 635, 833
MboI GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 673, 865
MluCI AATT 3 cut(s) 133, 222, 427
Mly113I GGCGCC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 33
MspI CCGG 2 cut(s) 461, 674
MspR9I CCNGG 2 cut(s) 462, 675
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 369
NarI GGCGCC 1 cut(s) 687
NciI CCSGG 2 cut(s) 462, 675
NdeII GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
NlaIII CATG 8 cut(s) 192, 216, 379, 435, 523, 640, 743, 755
NlaIV GGNNCC 2 cut(s) 561, 688
NmeAIII GCCGAG 2 cut(s) 339, 627
NmuCI GTSAC 5 cut(s) 105, 117, 271, 481, 854
NspI RCATGY 1 cut(s) 216
PceI AGGCCT 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 486
Pfl23II CGTACG 1 cut(s) 486
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 3 cut(s) 53, 313, 459
PluTI GGCGCC 1 cut(s) 690
PmaCI CACGTG 1 cut(s) 481
PmlI CACGTG 1 cut(s) 481
Ppu21I YACGTR 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 673
PspCI CACGTG 1 cut(s) 481
PspLI CGTACG 1 cut(s) 486
PspN4I GGNNCC 2 cut(s) 561, 688
PspPI GGNCC 2 cut(s) 6, 499
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 4 cut(s) 488, 614, 659, 809
RsaNI GTAC 4 cut(s) 487, 613, 658, 808
SacI GAGCTC 1 cut(s) 673
SatI GCNGC 3 cut(s) 52, 312, 458
Sau3AI GATC 9 cut(s) 89, 193, 315, 435, 444, 525, 559, 633, 831
Sau96I GGNCC 2 cut(s) 6, 499
ScrFI CCNGG 2 cut(s) 462, 675
SduI GDGCHC 2 cut(s) 673, 865
SfaNI GCATC 2 cut(s) 33, 634
SfoI GGCGCC 1 cut(s) 688
SinI GGWCC 2 cut(s) 6, 499
SmlI CTYRAG 2 cut(s) 32, 590
SmoI CTYRAG 2 cut(s) 32, 590
Sse9I AATT 3 cut(s) 133, 222, 427
SseBI AGGCCT 1 cut(s) 473
SsiI CCGC 6 cut(s) 14, 309, 312, 399, 509, 701
SspDI GGCGCC 1 cut(s) 686
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 1 cut(s) 673
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 2 cut(s) 460, 673
StyI CCWWGG 2 cut(s) 495, 786
TaaI ACNGT 3 cut(s) 117, 123, 409
TaiI ACGT 3 cut(s) 273, 483, 488
TaqI TCGA 6 cut(s) 366, 417, 438, 621, 663, 865
TasI AATT 3 cut(s) 133, 222, 427
TauI GCSGC 1 cut(s) 314
TseFI GTSAC 5 cut(s) 105, 117, 271, 481, 854
TseI GCWGC 2 cut(s) 51, 457
Tsp45I GTSAC 5 cut(s) 105, 117, 271, 481, 854
TspDTI ATGAA 3 cut(s) 49, 70, 420
TspGWI ACGGA 3 cut(s) 292, 681, 830
VpaK11BI GGWCC 2 cut(s) 6, 499
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 874
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.