MD08G1215400.v1.1

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009914
Physical Location & Seq
Forward (+)
27836990 .. 27837874
885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215400.v1.1.491

Sequence Viewer

Length: 885 bp
ATGAGGTCCAAACTTCAATGTGGCATCAATCTGAAGGTCATCCTCTTCTGCTGCTTCTTCATCTTCCTCCTCACCATCTTCATAACCAGATCAACCTTCTCTTCATCATCACCACCATCTTCATCCACCAATTCCTCGCAATCCCATCTCATCTCAACCTCTAAACCCCAACAACAACAACCGTGCGATCAGCCCTTCATCCCAACATGCACCAAAACCCCACCATCCCTCGCCCACGCCATCATCCACTACGCAACCACAAACGTCACCCCACAACAAACCATGAAAGAAGTCTCCGTCTCCGCCGCGGTCCTTTCCCAAAAATCACCCTGCAACTTTCTAGTCTTCGGCCTCGGCTTTGACAGCCTCATGTGGACGGCTCTCAACCACGGCGGAAGAACCATCTTCCTCGAAGAAGACAATTCATGGATCGACCAGATCAAACAGAAGCTGCCGGGGTTGGAGGCCTACCACGTGAAGTACGACACCAAGGTTCACCAAGCGGACAAGCTCATGGAGATGGGAAGGAAGGAAGAGTGCAGGGCTGTCGGGGATCCAAGGTTTTCAAAGTGCGAGTTGGCGCTCAAGAACTTGCCGAGCGATGTGTACGATATAGAGTGGGATTTGATCATGGTGGATGCGCCGACGGGGTACTTCGACGGAGCTCCGGGAAGAATGAGCGCCATATACACGGCGGGGCTGCTGGGGAGGAACAGGGAGGAGGGGGAAGCTCATGTGTTTGTGCATGATGTGGATAGAGTGGTTGAGGACAGCTTCTCCAAGGCATTTCTCTGTGAGGGGTACTTGACGGAGCAGGAGGGCAGGATCAGGCACTTCACTATTCCCAGTCACAGGGCTCGATTAGGTAGACCGTTCTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

295

Amino Acids

32.99

Weight (kDa)

6.79

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 96 - 279 2.7e-78 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 868
AccII CGCG 1 cut(s) 308
AciI CCGC 6 cut(s) 303, 306, 308, 393, 503, 695
AclWI GGATC 4 cut(s) 437, 548, 561, 833
AcuI CTGAAG 1 cut(s) 53
AcvI CACGTG 1 cut(s) 475
AfaI GTAC 4 cut(s) 482, 608, 653, 803
AfiI CCNNNNNNNGG 1 cut(s) 852
AgsI TTSAA 2 cut(s) 17, 567
AluBI AGCT 5 cut(s) 451, 511, 665, 731, 774
AluI AGCT 5 cut(s) 451, 511, 665, 731, 774
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 2 cut(s) 298, 304
AlwI GGATC 4 cut(s) 437, 548, 561, 833
AlwNI CAGNNNCTG 1 cut(s) 451
AoxI GGCC 2 cut(s) 349, 465
ApeKI GCWGC 3 cut(s) 51, 451, 700
AspLEI GCGC 3 cut(s) 583, 643, 683
AspS9I GGNCC 2 cut(s) 6, 310
AsuC2I CCSGG 2 cut(s) 456, 669
AsuHPI GGTGA 5 cut(s) 64, 102, 259, 318, 488
AvaII GGWCC 2 cut(s) 6, 310
BamHI GGATCC 1 cut(s) 553
BanII GRGCYC 2 cut(s) 667, 859
BbrPI CACGTG 1 cut(s) 475
BbsI GAAGAC 2 cut(s) 337, 423
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 3 cut(s) 38, 438, 687
BccI CCATC 7 cut(s) 83, 124, 153, 232, 248, 410, 514
BceAI ACGGC 3 cut(s) 393, 406, 708
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 2 cut(s) 456, 669
BcoDI GTCTC 2 cut(s) 298, 304
BfaI CTAG 1 cut(s) 341
BfoI RGCGCY 2 cut(s) 584, 684
BisI GCNGC 4 cut(s) 52, 306, 452, 701
BlsI GCNGC 4 cut(s) 53, 307, 453, 702
Bme1390I CCNGG 2 cut(s) 456, 669
Bme18I GGWCC 2 cut(s) 6, 310
BmgT120I GGNCC 2 cut(s) 6, 310
BmiI GGNNCC 1 cut(s) 555
BmrFI CCNGG 2 cut(s) 456, 669
BmrI ACTGGG 1 cut(s) 840
BmsI GCATC 2 cut(s) 33, 628
BmuI ACTGGG 1 cut(s) 840
BpiI GAAGAC 2 cut(s) 337, 423
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 2 cut(s) 456, 669
BsaAI YACGTR 1 cut(s) 475
BsaJI CCNNGG 7 cut(s) 306, 352, 388, 455, 489, 557, 780
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 1 cut(s) 852
Bse1I ACTGG 1 cut(s) 846
BseDI CCNNGG 7 cut(s) 306, 352, 388, 455, 489, 557, 780
BseGI GGATG 6 cut(s) 39, 122, 198, 224, 243, 643
BseLI CCNNNNNNNGG 1 cut(s) 852
BseNI ACTGG 1 cut(s) 846
BseRI GAGGAG 2 cut(s) 59, 734
BseXI GCAGC 3 cut(s) 38, 438, 687
BseYI CCCAGC 1 cut(s) 703
BsgI GTGCAG 1 cut(s) 559
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 351, 467
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 2 cut(s) 455, 668
BslI CCNNNNNNNGG 1 cut(s) 852
BsmAI GTCTC 2 cut(s) 298, 304
BsmBI CGTCTC 1 cut(s) 304
BsnI GGCC 2 cut(s) 351, 467
Bsp1286I GDGCHC 2 cut(s) 667, 859
Bsp143I GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
BspACI CCGC 6 cut(s) 303, 306, 308, 393, 503, 695
BspANI GGCC 2 cut(s) 351, 467
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 555
BspPI GGATC 4 cut(s) 437, 548, 561, 833
BsrI ACTGG 1 cut(s) 846
BssECI CCNNGG 7 cut(s) 306, 352, 388, 455, 489, 557, 780
BssMI GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
BssT1I CCWWGG 3 cut(s) 489, 557, 780
Bst4CI ACNGT 2 cut(s) 183, 873
Bst6I CTCTTC 3 cut(s) 50, 106, 528
BstBAI YACGTR 1 cut(s) 475
BstDEI CTNAG 1 cut(s) 882
BstDSI CCRYGG 2 cut(s) 306, 388
BstF5I GGATG 6 cut(s) 39, 122, 198, 224, 243, 643
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 2 cut(s) 584, 684
BstHHI GCGC 3 cut(s) 583, 643, 683
BstKTI GATC 7 cut(s) 92, 190, 432, 441, 556, 630, 828
BstMAI GTCTC 2 cut(s) 298, 304
BstMBI GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
BstMWI GCNNNNNNNGC 1 cut(s) 363
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 2 cut(s) 454, 667
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 3 cut(s) 38, 438, 687
BstV2I GAAGAC 2 cut(s) 337, 423
BstX2I RGATCY 1 cut(s) 553
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 2 cut(s) 351, 467
BtgI CCRYGG 2 cut(s) 306, 388
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 6 cut(s) 39, 122, 198, 224, 243, 643
CaiI CAGNNNCTG 1 cut(s) 451
CfoI GCGC 3 cut(s) 583, 643, 683
Cfr13I GGNCC 2 cut(s) 6, 310
Cfr42I CCGCGG 1 cut(s) 309
Csp6I GTAC 4 cut(s) 481, 607, 652, 802
CviAII CATG 8 cut(s) 207, 283, 370, 426, 514, 631, 734, 746
CviQI GTAC 4 cut(s) 481, 607, 652, 802
DdeI CTNAG 1 cut(s) 882
DpnI GATC 7 cut(s) 91, 189, 431, 440, 555, 629, 827
DpnII GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
Eam1104I CTCTTC 3 cut(s) 50, 106, 528
EarI CTCTTC 3 cut(s) 50, 106, 528
EciI GGCGGA 2 cut(s) 292, 408
Ecl136II GAGCTC 1 cut(s) 665
Eco130I CCWWGG 3 cut(s) 489, 557, 780
Eco147I AGGCCT 1 cut(s) 467
Eco24I GRGCYC 2 cut(s) 667, 859
Eco47I GGWCC 2 cut(s) 6, 310
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 1 cut(s) 53
Eco72I CACGTG 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 665
EcoT14I CCWWGG 3 cut(s) 489, 557, 780
EcoT38I GRGCYC 2 cut(s) 667, 859
ErhI CCWWGG 3 cut(s) 489, 557, 780
Esp3I CGTCTC 1 cut(s) 304
FaeI CATG 8 cut(s) 210, 286, 373, 429, 517, 634, 737, 749
FatI CATG 8 cut(s) 206, 282, 369, 425, 513, 630, 733, 745
FauI CCCGC 1 cut(s) 688
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 1 cut(s) 868
Fnu4HI GCNGC 4 cut(s) 52, 306, 452, 701
FokI GGATG 6 cut(s) 26, 109, 185, 211, 230, 650
FriOI GRGCYC 2 cut(s) 667, 859
Fsp4HI GCNGC 4 cut(s) 52, 306, 452, 701
FspBI CTAG 1 cut(s) 341
GlaI GCGC 3 cut(s) 582, 642, 682
GluI GCNGC 4 cut(s) 52, 306, 452, 701
GsaI CCCAGC 1 cut(s) 707
HaeII RGCGCY 2 cut(s) 584, 684
HaeIII GGCC 2 cut(s) 351, 467
HapII CCGG 2 cut(s) 455, 668
HhaI GCGC 3 cut(s) 583, 643, 683
Hin1II CATG 8 cut(s) 210, 286, 373, 429, 517, 634, 737, 749
Hin6I GCGC 3 cut(s) 581, 641, 681
HinP1I GCGC 3 cut(s) 581, 641, 681
HpaII CCGG 2 cut(s) 455, 668
HphI GGTGA 5 cut(s) 64, 102, 259, 318, 488
Hpy166II GTNNAC 4 cut(s) 375, 496, 607, 869
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 1 cut(s) 586
Hpy8I GTNNAC 4 cut(s) 375, 496, 607, 869
Hpy99I CGWCG 2 cut(s) 649, 662
HpyAV CCTTC 5 cut(s) 28, 106, 205, 519, 523
HpyCH4III ACNGT 2 cut(s) 183, 873
HpyCH4IV ACGT 2 cut(s) 264, 474
HpyCH4V TGCA 4 cut(s) 210, 333, 540, 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 882
HpySE526I ACGT 2 cut(s) 264, 474
Hsp92II CATG 8 cut(s) 210, 286, 373, 429, 517, 634, 737, 749
HspAI GCGC 3 cut(s) 581, 641, 681
Ksp22I TGATCA 1 cut(s) 627
KspI CCGCGG 1 cut(s) 309
Kzo9I GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
LmnI GCTCC 3 cut(s) 662, 670, 811
Lsp1109I GCAGC 3 cut(s) 38, 438, 687
LweI GCATC 2 cut(s) 33, 628
MaeI CTAG 1 cut(s) 341
MaeII ACGT 2 cut(s) 264, 474
MaeIII GTNAC 2 cut(s) 265, 848
MalI GATC 7 cut(s) 91, 189, 431, 440, 555, 629, 827
MboI GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 2 cut(s) 667, 859
MluCI AATT 2 cut(s) 130, 421
MmeI TCCRAC 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 455, 668
MspR9I CCNGG 2 cut(s) 456, 669
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 363
NciI CCSGG 2 cut(s) 456, 669
NdeII GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
NlaIII CATG 8 cut(s) 210, 286, 373, 429, 517, 634, 737, 749
NlaIV GGNNCC 1 cut(s) 555
NmeAIII GCCGAG 2 cut(s) 333, 621
NmuCI GTSAC 2 cut(s) 265, 848
NspI RCATGY 1 cut(s) 210
PceI AGGCCT 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 4 cut(s) 53, 307, 453, 702
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 475
Psp124BI GAGCTC 1 cut(s) 667
PspCI CACGTG 1 cut(s) 475
PspFI CCCAGC 1 cut(s) 703
PspN4I GGNNCC 1 cut(s) 555
PspPI GGNCC 2 cut(s) 6, 310
PstNI CAGNNNCTG 1 cut(s) 451
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 4 cut(s) 482, 608, 653, 803
RsaNI GTAC 4 cut(s) 481, 607, 652, 802
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 667
SacII CCGCGG 1 cut(s) 309
SatI GCNGC 4 cut(s) 52, 306, 452, 701
Sau3AI GATC 7 cut(s) 89, 187, 429, 438, 553, 627, 825
Sau96I GGNCC 2 cut(s) 6, 310
ScrFI CCNGG 2 cut(s) 456, 669
SduI GDGCHC 2 cut(s) 667, 859
SfaNI GCATC 2 cut(s) 33, 628
Sfr303I CCGCGG 1 cut(s) 309
SgrBI CCGCGG 1 cut(s) 309
SinI GGWCC 2 cut(s) 6, 310
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 2 cut(s) 130, 421
SseBI AGGCCT 1 cut(s) 467
SsiI CCGC 6 cut(s) 303, 306, 308, 393, 503, 695
SspMI CTAG 1 cut(s) 341
SstI GAGCTC 1 cut(s) 667
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 454, 667
StyI CCWWGG 3 cut(s) 489, 557, 780
TaaI ACNGT 2 cut(s) 183, 873
TaiI ACGT 2 cut(s) 267, 477
TaqI TCGA 4 cut(s) 411, 432, 657, 859
TasI AATT 2 cut(s) 130, 421
TauI GCSGC 1 cut(s) 308
TseFI GTSAC 2 cut(s) 265, 848
TseI GCWGC 3 cut(s) 51, 451, 700
Tsp45I GTSAC 2 cut(s) 265, 848
TspDTI ATGAA 7 cut(s) 49, 70, 93, 111, 187, 299, 414
TspGWI ACGGA 3 cut(s) 286, 675, 824
VpaK11BI GGWCC 2 cut(s) 6, 310
XceI RCATGY 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 868
XspI CTAG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.