MD03G1203200.v1.1

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009914
Physical Location & Seq
Reverse (-)
27722646 .. 27723113
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1203200.v1.1.491

Sequence Viewer

Length: 468 bp
ATGTGCAGCTTCAGAGATGTCATTCATGGCAACACTAAGGTCGTTCACAAAGCTAACCACTCGTCGCCATCCCTGGCCCATGCCATCATCCACTACACGACCATAAACGTCACCCCACAACGAACCATGAAAGAAGTCTCCGTCTCCGCTGCAGTCCTTTCCCAAAAATCACCCTGCAACTTTCTAGTTTTTGGCCTCGGCTTTGACAACCTCATATGGACGGCTCTCAACCACGGCGGAAGAACCATCTTCCTCGAAGAAGACAATTCATGGATTGACCAGATCAAACAGAAGCTACCGGGTTTGGAGGCTTACCACGTGGAGTACGACACCAAGGTTCACCAAGCGGATAAGCTCATGGAGATGGGAAGGAAGGAAGAGTGCAGGGCTGTCGAGGATCCAAGGTTTACATTCTTTACAGCTGGAGTACACCAGCTTGTGTTCAAACAGCAGAAAAGAGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

156

Amino Acids

17.71

Weight (kDa)

8.45

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 44 - 132 2.6e-32 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 147, 237, 347
AclWI GGATC 2 cut(s) 392, 405
AcvI CACGTG 1 cut(s) 319
AfaI GTAC 2 cut(s) 326, 429
AgsI TTSAA 1 cut(s) 445
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 6 cut(s) 9, 53, 295, 355, 422, 436
AluI AGCT 6 cut(s) 9, 53, 295, 355, 422, 436
Alw26I GTCTC 2 cut(s) 142, 148
AlwI GGATC 2 cut(s) 392, 405
AoxI GGCC 2 cut(s) 75, 193
ApeKI GCWGC 2 cut(s) 6, 149
AspS9I GGNCC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 3 cut(s) 103, 162, 332
BamHI GGATCC 1 cut(s) 397
BbrPI CACGTG 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 267
BbvI GCAGC 2 cut(s) 18, 136
BccI CCATC 4 cut(s) 76, 92, 254, 358
BceAI ACGGC 2 cut(s) 237, 250
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 142, 148
BfaI CTAG 1 cut(s) 185
BfmI CTRYAG 1 cut(s) 150
BisI GCNGC 2 cut(s) 7, 150
BlsI GCNGC 2 cut(s) 8, 151
Bme1390I CCNGG 2 cut(s) 74, 300
BmgT120I GGNCC 1 cut(s) 76
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 2 cut(s) 74, 300
BpiI GAAGAC 1 cut(s) 267
BpmI CTGGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 319
BsaJI CCNNGG 5 cut(s) 72, 196, 232, 333, 401
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 5 cut(s) 72, 196, 232, 333, 401
BseGI GGATG 2 cut(s) 68, 87
BseXI GCAGC 2 cut(s) 18, 136
BsgI GTGCAG 2 cut(s) 25, 403
BshFI GGCC 2 cut(s) 77, 195
BsiSI CCGG 1 cut(s) 299
BsmAI GTCTC 2 cut(s) 142, 148
BsmBI CGTCTC 1 cut(s) 148
BsnI GGCC 2 cut(s) 77, 195
Bsp143I GATC 2 cut(s) 282, 397
BspACI CCGC 3 cut(s) 147, 237, 347
BspANI GGCC 2 cut(s) 77, 195
BspLI GGNNCC 1 cut(s) 399
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 2 cut(s) 392, 405
BssECI CCNNGG 5 cut(s) 72, 196, 232, 333, 401
BssMI GATC 2 cut(s) 282, 397
BssT1I CCWWGG 2 cut(s) 333, 401
Bst2UI CCWGG 1 cut(s) 74
Bst6I CTCTTC 1 cut(s) 372
BstBAI YACGTR 1 cut(s) 319
BstDEI CTNAG 1 cut(s) 36
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 68, 87
BstKTI GATC 2 cut(s) 285, 400
BstMAI GTCTC 2 cut(s) 142, 148
BstMBI GATC 2 cut(s) 282, 397
BstNI CCWGG 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 72, 298
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 18, 136
BstV2I GAAGAC 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 397
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 2 cut(s) 77, 195
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 2 cut(s) 68, 87
Cfr13I GGNCC 1 cut(s) 76
Csp6I GTAC 2 cut(s) 325, 428
CviAII CATG 5 cut(s) 26, 80, 127, 270, 358
CviQI GTAC 2 cut(s) 325, 428
DdeI CTNAG 1 cut(s) 36
DpnI GATC 2 cut(s) 284, 399
DpnII GATC 2 cut(s) 282, 397
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 333, 401
Eco72I CACGTG 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 333, 401
ErhI CCWWGG 2 cut(s) 333, 401
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 5 cut(s) 29, 83, 130, 273, 361
FaiI YATR 8 cut(s) 27, 81, 104, 128, 215, 217, 271, 359
FatI CATG 5 cut(s) 25, 79, 126, 269, 357
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 2 cut(s) 7, 150
FokI GGATG 2 cut(s) 55, 74
Fsp4HI GCNGC 2 cut(s) 7, 150
FspBI CTAG 1 cut(s) 185
GluI GCNGC 2 cut(s) 7, 150
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 2 cut(s) 77, 195
HapII CCGG 1 cut(s) 299
Hin1II CATG 5 cut(s) 29, 83, 130, 273, 361
HpaII CCGG 1 cut(s) 299
HphI GGTGA 3 cut(s) 103, 162, 332
Hpy166II GTNNAC 4 cut(s) 46, 340, 408, 430
Hpy188I TCNGA 1 cut(s) 14
Hpy8I GTNNAC 4 cut(s) 46, 340, 408, 430
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 363, 367
HpyCH4IV ACGT 2 cut(s) 108, 318
HpyCH4V TGCA 4 cut(s) 6, 152, 177, 384
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 2 cut(s) 108, 318
Hsp92II CATG 5 cut(s) 29, 83, 130, 273, 361
Kzo9I GATC 2 cut(s) 282, 397
LpnPI CCDG 8 cut(s) 59, 86, 187, 293, 312, 370, 408, 446
Lsp1109I GCAGC 2 cut(s) 18, 136
MaeI CTAG 1 cut(s) 185
MaeII ACGT 2 cut(s) 108, 318
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 284, 399
MboI GATC 2 cut(s) 282, 397
MboII GAAGA 5 cut(s) 241, 252, 269, 272, 389
MflI RGATCY 1 cut(s) 397
MluCI AATT 1 cut(s) 265
MnlI CCTC 5 cut(s) 206, 221, 263, 301, 388
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 149, 422
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 74, 300
MvaI CCWGG 1 cut(s) 74
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 215
NdeII GATC 2 cut(s) 282, 397
NlaIII CATG 5 cut(s) 29, 83, 130, 273, 361
NlaIV GGNNCC 1 cut(s) 399
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PkrI GCNGC 2 cut(s) 8, 151
PmaCI CACGTG 1 cut(s) 319
PmlI CACGTG 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 72
PspCI CACGTG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 1 cut(s) 76
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 2 cut(s) 326, 429
RsaNI GTAC 2 cut(s) 325, 428
RseI CAYNNNNRTG 1 cut(s) 362
SatI GCNGC 2 cut(s) 7, 150
Sau3AI GATC 2 cut(s) 282, 397
Sau96I GGNCC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 74, 300
SfcI CTRYAG 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 362
Sse9I AATT 1 cut(s) 265
SsiI CCGC 3 cut(s) 147, 237, 347
SspMI CTAG 1 cut(s) 185
StyD4I CCNGG 2 cut(s) 72, 298
StyI CCWWGG 2 cut(s) 333, 401
TaiI ACGT 2 cut(s) 111, 321
TaqI TCGA 2 cut(s) 255, 393
TasI AATT 1 cut(s) 265
TatI WGTACW 1 cut(s) 427
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 6, 149
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 3 cut(s) 14, 143, 258
TspGWI ACGGA 1 cut(s) 130
XspI CTAG 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.