pycom15g35850

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009914
Physical Location & Seq
Forward (+)
35930792 .. 35931965
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35850.1

Sequence Viewer

Length: 1110 bp
ATGGATCTGGTAATATGCCATCATCGCTTGAGTAACTCTCAAGGGTCAGAAACAAACAATAAACCCCTGTCTCCTCCCTCTCTTCCATTCCCCACCACTTCTCCACCTTCTGTTCATAAAACCTCAGATCAACATTATCTCCTCGGTCCCCCCTCTCTCTCTCTCTCTCCTCTCTCTCTCTCTCTCATCTCATCAAGCACCATGAGGTCCAAACCTCAATGTGGCATCAGTCTCAAGGTCATCCTCCTCTGCTGCTGCTTCATCTTCCTCTTCACTGTCTTCATTGTCAGATCAACCTTCTCATCGTCACTATTTCCGTCACCGTCCACGGCCAATTCCTCACTATCCCATCTCATGCCAAACCCTAAACCCCAACAACAACAACAACAACAACAACAACAACCACCATGCGATCAACCATTCACCCCAACATGCACCAAAATCCCACCATCCCTAGCCCACGCCATCATCCACTACGCCACAACAAACGTCACGCCACAACAAACCATCAAAGAAGTCTCCGTCTCCGCCGCGATCCTTTCCAAAAAATCACCCTGCAACTTTCTAGTCTTCGGCCTCGGCCGCGACAGCCTCATGTGGACGGCTCTCAACCACGGCGGAAGAACCATCTTCCTCGAAGAAGACAATTCATGGATCGACCAAATCAAACAGAAGCTGCCGGGCTTAGAGGCCTACCACGTGACATACGACACCAAGGTCCACCAAGCGGACAAGCTCATGGAGATCGGAAGGAAGGAAGAGTGCAAAGCTGTTGGGGATCCGAGGTTTTCAAAGTGCGAGCTGGCGCTCAAGAACTTGCCGAGCGATGTGTACGATATCGAGTGGGATCTGATCATGGTGGATGCGCCGACGGGGTACTTCGACGGAGCTCCGGGAAGAATGGGCGCCATATACACGGCGGGGCTGATGGGGAGGAACAGGGAGGAGGGGGAAACTCATGTGTTTGTACATGATGTGAATAGAGTGGTTGAGGACAGCTTCTCCAAGGCTTTTCTCTGCGAGGGGTACTTGACGGAGCAGGAGGGCCTGCTCAGGCACTTCACTATTCCCAGTCACAGGGCTCGATTAGGTAGACCATTTTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

370

Amino Acids

40.97

Weight (kDa)

7.2

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 171 - 354 8e-79 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 905
AccI GTMKAC 1 cut(s) 1093
AccII CGCG 2 cut(s) 533, 585
AciI CCGC 6 cut(s) 528, 531, 583, 618, 728, 920
AclWI GGATC 6 cut(s) 12, 529, 662, 773, 786, 855
AcoI YGGCCR 2 cut(s) 330, 580
AcvI CACGTG 1 cut(s) 700
AcyI GRCGYC 1 cut(s) 906
AfaI GTAC 4 cut(s) 833, 878, 969, 1028
AfiI CCNNNNNNNGG 3 cut(s) 221, 727, 1077
AgsI TTSAA 1 cut(s) 792
AluBI AGCT 6 cut(s) 676, 736, 770, 802, 890, 999
AluI AGCT 6 cut(s) 676, 736, 770, 802, 890, 999
Alw21I GWGCWC 1 cut(s) 892
Alw26I GTCTC 4 cut(s) 75, 236, 523, 529
AlwI GGATC 6 cut(s) 12, 529, 662, 773, 786, 855
AlwNI CAGNNNCTG 1 cut(s) 676
AoxI GGCC 5 cut(s) 330, 574, 580, 690, 1045
ApeKI GCWGC 3 cut(s) 252, 255, 676
AspLEI GCGC 3 cut(s) 808, 868, 908
AspS9I GGNCC 4 cut(s) 146, 207, 718, 1045
AsuC2I CCSGG 2 cut(s) 681, 894
AsuHPI GGTGA 3 cut(s) 312, 415, 543
AvaII GGWCC 3 cut(s) 146, 207, 718
BamHI GGATCC 1 cut(s) 778
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 2 cut(s) 892, 1084
BbrPI CACGTG 1 cut(s) 700
BbsI GAAGAC 3 cut(s) 271, 562, 648
Bbv12I GWGCWC 1 cut(s) 892
BbvI GCAGC 3 cut(s) 239, 242, 663
BccI CCATC 7 cut(s) 27, 357, 457, 473, 515, 635, 922
BceAI ACGGC 4 cut(s) 345, 618, 631, 933
BclI TGATCA 1 cut(s) 852
BcnI CCSGG 2 cut(s) 681, 894
BcoDI GTCTC 4 cut(s) 75, 236, 523, 529
BfaI CTAG 2 cut(s) 455, 566
BfoI RGCGCY 2 cut(s) 809, 909
BisI GCNGC 5 cut(s) 253, 256, 531, 583, 677
BlsI GCNGC 5 cut(s) 254, 257, 532, 584, 678
Bme1390I CCNGG 2 cut(s) 681, 894
Bme18I GGWCC 3 cut(s) 146, 207, 718
BmgT120I GGNCC 4 cut(s) 146, 207, 718, 1045
BmiI GGNNCC 3 cut(s) 148, 780, 907
BmrFI CCNGG 2 cut(s) 681, 894
BmrI ACTGGG 1 cut(s) 1065
BmsI GCATC 2 cut(s) 234, 853
BmuI ACTGGG 1 cut(s) 1065
BpiI GAAGAC 3 cut(s) 271, 562, 648
BplI GAGNNNNNCTC 2 cut(s) 22, 54
Bpu10I CCTNAGC 1 cut(s) 1052
BpuEI CTTGAG 4 cut(s) 24, 49, 218, 794
BpuMI CCSGG 2 cut(s) 681, 894
BsaAI YACGTR 1 cut(s) 700
BsaHI GRCGYC 1 cut(s) 906
BsaJI CCNNGG 7 cut(s) 142, 327, 577, 613, 714, 782, 1005
BsaXI ACNNNNNCTCC 6 cut(s) 85, 115, 123, 153, 986, 1016
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 727, 1077
Bse1I ACTGG 1 cut(s) 1071
BseDI CCNNGG 7 cut(s) 142, 327, 577, 613, 714, 782, 1005
BseGI GGATG 4 cut(s) 240, 449, 468, 868
BseLI CCNNNNNNNGG 3 cut(s) 221, 727, 1077
BseMII CTCAG 2 cut(s) 138, 1066
BseNI ACTGG 1 cut(s) 1071
BseRI GAGGAG 5 cut(s) 63, 131, 159, 236, 959
BseX3I CGGCCG 1 cut(s) 580
BseXI GCAGC 3 cut(s) 239, 242, 663
Bsh1236I CGCG 2 cut(s) 533, 585
Bsh1285I CGRYCG 1 cut(s) 583
BshFI GGCC 5 cut(s) 332, 576, 582, 692, 1047
BshNI GGYRCC 1 cut(s) 905
BsiEI CGRYCG 1 cut(s) 583
BsiHKAI GWGCWC 1 cut(s) 892
BsiSI CCGG 2 cut(s) 680, 893
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 3 cut(s) 221, 727, 1077
BsmAI GTCTC 4 cut(s) 75, 236, 523, 529
BsmBI CGTCTC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 132
BsnI GGCC 5 cut(s) 332, 576, 582, 692, 1047
Bsp1286I GDGCHC 2 cut(s) 892, 1084
Bsp1407I TGTACA 1 cut(s) 967
BspACI CCGC 6 cut(s) 528, 531, 583, 618, 728, 920
BspANI GGCC 5 cut(s) 332, 576, 582, 692, 1047
BspCNI CTCAG 2 cut(s) 137, 1065
BspFNI CGCG 2 cut(s) 533, 585
BspLI GGNNCC 3 cut(s) 148, 780, 907
BspPI GGATC 6 cut(s) 12, 529, 662, 773, 786, 855
BspT107I GGYRCC 1 cut(s) 905
BsrGI TGTACA 1 cut(s) 967
BsrI ACTGG 1 cut(s) 1071
BssECI CCNNGG 7 cut(s) 142, 327, 577, 613, 714, 782, 1005
BssNI GRCGYC 1 cut(s) 906
BssT1I CCWWGG 2 cut(s) 714, 1005
Bst4CI ACNGT 2 cut(s) 277, 324
Bst6I CTCTTC 3 cut(s) 87, 275, 753
BstACI GRCGYC 1 cut(s) 906
BstAUI TGTACA 1 cut(s) 967
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 3 cut(s) 800, 804, 1049
BstDEI CTNAG 4 cut(s) 124, 685, 1052, 1107
BstDSI CCRYGG 2 cut(s) 327, 613
BstF5I GGATG 4 cut(s) 240, 449, 468, 868
BstFNI CGCG 2 cut(s) 533, 585
BstH2I RGCGCY 2 cut(s) 809, 909
BstHHI GCGC 3 cut(s) 808, 868, 908
BstMAI GTCTC 4 cut(s) 75, 236, 523, 529
BstMCI CGRYCG 1 cut(s) 583
BstMWI GCNNNNNNNGC 3 cut(s) 24, 582, 588
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 679, 892
BstUI CGCG 2 cut(s) 533, 585
BstV1I GCAGC 3 cut(s) 239, 242, 663
BstV2I GAAGAC 3 cut(s) 271, 562, 648
BstX2I RGATCY 3 cut(s) 4, 778, 847
BstYI RGATCY 3 cut(s) 4, 778, 847
BstZI CGGCCG 1 cut(s) 580
BsuRI GGCC 5 cut(s) 332, 576, 582, 692, 1047
BtgI CCRYGG 2 cut(s) 327, 613
BtgZI GCGATG 2 cut(s) 8, 840
BtsCI GGATG 4 cut(s) 240, 449, 468, 868
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 800, 804, 1049
CaiI CAGNNNCTG 1 cut(s) 676
CfoI GCGC 3 cut(s) 808, 868, 908
Cfr13I GGNCC 4 cut(s) 146, 207, 718, 1045
Csp6I GTAC 4 cut(s) 832, 877, 968, 1027
CviQI GTAC 4 cut(s) 832, 877, 968, 1027
DdeI CTNAG 4 cut(s) 124, 685, 1052, 1107
DinI GGCGCC 1 cut(s) 907
DrdI GACNNNNNNGTC 1 cut(s) 716
DseDI GACNNNNNNGTC 1 cut(s) 716
EaeI YGGCCR 2 cut(s) 330, 580
EagI CGGCCG 1 cut(s) 580
Eam1104I CTCTTC 3 cut(s) 87, 275, 753
EarI CTCTTC 3 cut(s) 87, 275, 753
EciI GGCGGA 2 cut(s) 517, 633
Ecl136II GAGCTC 1 cut(s) 890
EclXI CGGCCG 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 714, 1005
Eco147I AGGCCT 1 cut(s) 692
Eco24I GRGCYC 2 cut(s) 892, 1084
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 3 cut(s) 146, 207, 718
Eco52I CGGCCG 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 890
Eco72I CACGTG 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 1045
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 714, 1005
EcoT38I GRGCYC 2 cut(s) 892, 1084
EgeI GGCGCC 1 cut(s) 907
EheI GGCGCC 1 cut(s) 907
ErhI CCWWGG 2 cut(s) 714, 1005
Esp3I CGTCTC 1 cut(s) 529
FaqI GGGAC 1 cut(s) 132
FauI CCCGC 1 cut(s) 913
FbaI TGATCA 1 cut(s) 852
FblI GTMKAC 1 cut(s) 1093
Fnu4HI GCNGC 5 cut(s) 253, 256, 531, 583, 677
FokI GGATG 4 cut(s) 227, 436, 455, 875
FriOI GRGCYC 2 cut(s) 892, 1084
Fsp4HI GCNGC 5 cut(s) 253, 256, 531, 583, 677
FspBI CTAG 2 cut(s) 455, 566
GlaI GCGC 3 cut(s) 807, 867, 907
GluI GCNGC 5 cut(s) 253, 256, 531, 583, 677
HaeII RGCGCY 2 cut(s) 809, 909
HaeIII GGCC 5 cut(s) 332, 576, 582, 692, 1047
HapII CCGG 2 cut(s) 680, 893
HhaI GCGC 3 cut(s) 808, 868, 908
Hin1I GRCGYC 1 cut(s) 906
Hin6I GCGC 3 cut(s) 806, 866, 906
HinP1I GCGC 3 cut(s) 806, 866, 906
HpaII CCGG 2 cut(s) 680, 893
HphI GGTGA 3 cut(s) 312, 415, 543
Hpy166II GTNNAC 5 cut(s) 327, 600, 721, 832, 1094
Hpy188I TCNGA 6 cut(s) 49, 127, 290, 749, 783, 852
Hpy188III TCNNGA 1 cut(s) 811
Hpy8I GTNNAC 5 cut(s) 327, 600, 721, 832, 1094
Hpy99I CGWCG 2 cut(s) 874, 887
HpyAV CCTTC 4 cut(s) 117, 307, 744, 748
HpyCH4III ACNGT 2 cut(s) 277, 324
HpyCH4IV ACGT 2 cut(s) 489, 699
HpyCH4V TGCA 3 cut(s) 435, 558, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 582, 588
HpyF3I CTNAG 4 cut(s) 124, 685, 1052, 1107
HpySE526I ACGT 2 cut(s) 489, 699
Hsp92I GRCGYC 1 cut(s) 906
HspAI GCGC 3 cut(s) 806, 866, 906
KasI GGCGCC 1 cut(s) 905
Ksp22I TGATCA 1 cut(s) 852
LmnI GCTCC 3 cut(s) 887, 895, 1036
Lsp1109I GCAGC 3 cut(s) 239, 242, 663
LweI GCATC 2 cut(s) 234, 853
MaeI CTAG 2 cut(s) 455, 566
MaeII ACGT 2 cut(s) 489, 699
MaeIII GTNAC 6 cut(s) 32, 306, 318, 490, 700, 1073
MflI RGATCY 3 cut(s) 4, 778, 847
MhlI GDGCHC 2 cut(s) 892, 1084
MluCI AATT 2 cut(s) 334, 646
Mly113I GGCGCC 1 cut(s) 906
MspI CCGG 2 cut(s) 680, 893
MspR9I CCNGG 2 cut(s) 681, 894
MvnI CGCG 2 cut(s) 533, 585
MwoI GCNNNNNNNGC 3 cut(s) 24, 582, 588
NarI GGCGCC 1 cut(s) 906
NciI CCSGG 2 cut(s) 681, 894
NlaIV GGNNCC 3 cut(s) 148, 780, 907
NmeAIII GCCGAG 2 cut(s) 558, 846
NmuCI GTSAC 5 cut(s) 306, 318, 490, 700, 1073
NspI RCATGY 1 cut(s) 435
PceI AGGCCT 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 5 cut(s) 254, 257, 532, 584, 678
PluTI GGCGCC 1 cut(s) 909
PmaCI CACGTG 1 cut(s) 700
PmlI CACGTG 1 cut(s) 700
Ppu21I YACGTR 1 cut(s) 700
Psp124BI GAGCTC 1 cut(s) 892
PspCI CACGTG 1 cut(s) 700
PspN4I GGNNCC 3 cut(s) 148, 780, 907
PspPI GGNCC 4 cut(s) 146, 207, 718, 1045
PstNI CAGNNNCTG 1 cut(s) 676
PsuI RGATCY 3 cut(s) 4, 778, 847
RsaI GTAC 4 cut(s) 833, 878, 969, 1028
RsaNI GTAC 4 cut(s) 832, 877, 968, 1027
SacI GAGCTC 1 cut(s) 892
SatI GCNGC 5 cut(s) 253, 256, 531, 583, 677
Sau96I GGNCC 4 cut(s) 146, 207, 718, 1045
ScrFI CCNGG 2 cut(s) 681, 894
SduI GDGCHC 2 cut(s) 892, 1084
SfaNI GCATC 2 cut(s) 234, 853
SfoI GGCGCC 1 cut(s) 907
SinI GGWCC 3 cut(s) 146, 207, 718
SmlI CTYRAG 4 cut(s) 28, 39, 233, 809
SmoI CTYRAG 4 cut(s) 28, 39, 233, 809
Sse9I AATT 2 cut(s) 334, 646
SseBI AGGCCT 1 cut(s) 692
SsiI CCGC 6 cut(s) 528, 531, 583, 618, 728, 920
SspDI GGCGCC 1 cut(s) 905
SspMI CTAG 2 cut(s) 455, 566
SstI GAGCTC 1 cut(s) 892
StuI AGGCCT 1 cut(s) 692
StyD4I CCNGG 2 cut(s) 679, 892
StyI CCWWGG 2 cut(s) 714, 1005
TaaI ACNGT 2 cut(s) 277, 324
TaiI ACGT 2 cut(s) 492, 702
TaqI TCGA 5 cut(s) 636, 657, 840, 882, 1084
TaqII GACCGA 1 cut(s) 134
TasI AATT 2 cut(s) 334, 646
TatI WGTACW 1 cut(s) 967
TauI GCSGC 2 cut(s) 533, 585
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 5 cut(s) 306, 318, 490, 700, 1073
TseI GCWGC 3 cut(s) 252, 255, 676
Tsp45I GTSAC 5 cut(s) 306, 318, 490, 700, 1073
TspDTI ATGAA 4 cut(s) 104, 250, 271, 639
TspGWI ACGGA 4 cut(s) 306, 511, 900, 1049
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 3 cut(s) 146, 207, 718
XceI RCATGY 1 cut(s) 435
XmiI GTMKAC 1 cut(s) 1093
XspI CTAG 2 cut(s) 455, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.