FvH4_5g33440

50S ribosomal protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005750
Physical Location & Seq
Forward (+)
24170716 .. 24171060
345 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33440.t1

Sequence Viewer

Length: 345 bp
ATGTCAGTCTCCGCTGTGATGTTGGGGCCTCAACTCCTCTCCATGCCCGTCCCTCTCCGCAACCCCTCCGCAAAGGCGTCGTTCGGACTCACCCAGATGGCGCCACCACTCTGCGGTGGTAGTACTGGGTTGGTCATAGAATGCTCGTCCAGGCCGCAGAAGAAGGCGACGGCTCACCACCGCAAGACCCGCCCACGCAAAACGGCTGCTTGGGACGTTAAACGTAAGCCCACCGTGTACGCTCCTCTGCCGCCTCTCCCCGCCGATTGGGTTTTCCTCAATTCTTCTGATGATGCTTCCGCTGCTGACCCTTCTCCTCCGCAAGAACAGGCCACTGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5323

Protein Analysis

115

Amino Acids

12.14

Weight (kDa)

9.83

Isoelectric Point (pI)

69.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5323 PF17257 40 - 99 1.1e-36 Family of unknown function (DUF5323)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 100
AcyI GRCGYC 2 cut(s) 77, 101
AfaI GTAC 2 cut(s) 124, 239
AfiI CCNNNNNNNGG 2 cut(s) 113, 267
AjnI CCWGG 1 cut(s) 149
Alw26I GTCTC 1 cut(s) 13
AoxI GGCC 3 cut(s) 26, 152, 330
ApeKI GCWGC 2 cut(s) 206, 302
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 82, 167
BanI GGYRCC 1 cut(s) 100
BbvI GCAGC 2 cut(s) 193, 289
BccI CCATC 1 cut(s) 91
BceAI ACGGC 2 cut(s) 186, 219
BcgI CGANNNNNNTGC 2 cut(s) 60, 94
BciT130I CCWGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 13
BfoI RGCGCY 1 cut(s) 104
BisI GCNGC 4 cut(s) 155, 207, 251, 303
BlsI GCNGC 4 cut(s) 156, 208, 252, 304
BmcAI AGTACT 1 cut(s) 124
Bme1390I CCNGG 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 27, 102
BmrFI CCNGG 1 cut(s) 151
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 135
BsaHI GRCGYC 2 cut(s) 77, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 267
Bse1I ACTGG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 113, 267
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 3 cut(s) 26, 234, 306
BseXI GCAGC 2 cut(s) 193, 289
BshFI GGCC 3 cut(s) 28, 154, 332
BshNI GGYRCC 1 cut(s) 100
BslFI GGGAC 2 cut(s) 35, 227
BslI CCNNNNNNNGG 2 cut(s) 113, 267
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 2 cut(s) 35, 227
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 3 cut(s) 28, 154, 332
BspANI GGCC 3 cut(s) 28, 154, 332
BspLI GGNNCC 2 cut(s) 27, 102
BspT107I GGYRCC 1 cut(s) 100
BsrI ACTGG 1 cut(s) 130
BssNI GRCGYC 2 cut(s) 77, 101
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 235, 337
BstACI GRCGYC 2 cut(s) 77, 101
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 189, 302
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 149
BstV1I GCAGC 2 cut(s) 193, 289
BsuRI GGCC 3 cut(s) 28, 154, 332
BtsIMutI CAGTG 1 cut(s) 333
CfoI GCGC 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 123, 238
CviAII CATG 1 cut(s) 43
CviJI RGCY 6 cut(s) 28, 154, 173, 206, 229, 332
CviKI_1 RGCY 6 cut(s) 28, 154, 173, 206, 229, 332
CviQI GTAC 2 cut(s) 123, 238
DinI GGCGCC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 149
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
FaeI CATG 1 cut(s) 46
FaiI YATR 2 cut(s) 44, 137
FaqI GGGAC 2 cut(s) 35, 227
FatI CATG 1 cut(s) 42
FauI CCCGC 2 cut(s) 197, 268
Fnu4HI GCNGC 4 cut(s) 155, 207, 251, 303
Fsp4HI GCNGC 4 cut(s) 155, 207, 251, 303
GlaI GCGC 1 cut(s) 102
GluI GCNGC 4 cut(s) 155, 207, 251, 303
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 3 cut(s) 28, 154, 332
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 103
Hin1I GRCGYC 2 cut(s) 77, 101
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HinfI GANTC 1 cut(s) 87
HphI GGTGA 2 cut(s) 82, 167
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 2 cut(s) 86, 289
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 2 cut(s) 82, 172
HpyAV CCTTC 2 cut(s) 157, 321
HpyCH4III ACNGT 2 cut(s) 235, 337
HpyCH4IV ACGT 2 cut(s) 216, 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 302
HpySE526I ACGT 2 cut(s) 216, 223
Hsp92I GRCGYC 2 cut(s) 77, 101
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 101
KasI GGCGCC 1 cut(s) 100
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 5 cut(s) 107, 111, 136, 163, 314
Lsp1109I GCAGC 2 cut(s) 193, 289
LweI GCATC 1 cut(s) 283
MaeII ACGT 2 cut(s) 216, 223
MboII GAAGA 2 cut(s) 172, 276
MluCI AATT 1 cut(s) 280
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 1 cut(s) 81
MnlI CCTC 8 cut(s) 39, 47, 63, 76, 255, 264, 287, 327
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 14, 302
MspR9I CCNGG 1 cut(s) 151
Mva1269I GAATGC 1 cut(s) 146
MvaI CCWGG 1 cut(s) 151
MwoI GCNNNNNNNGC 2 cut(s) 189, 302
NarI GGCGCC 1 cut(s) 101
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 27, 102
PctI GAATGC 1 cut(s) 146
PkrI GCNGC 4 cut(s) 156, 208, 252, 304
PleI GAGTC 1 cut(s) 81
PluTI GGCGCC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 149
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 2 cut(s) 27, 102
PspPI GGNCC 1 cut(s) 26
RsaI GTAC 2 cut(s) 124, 239
RsaNI GTAC 2 cut(s) 123, 238
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 4 cut(s) 155, 207, 251, 303
Sau96I GGNCC 1 cut(s) 26
ScaI AGTACT 1 cut(s) 124
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 151
SetI ASST 2 cut(s) 219, 226
SfaNI GCATC 1 cut(s) 283
SfoI GGCGCC 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 95
Sse9I AATT 1 cut(s) 280
SspDI GGCGCC 1 cut(s) 100
StyD4I CCNGG 1 cut(s) 149
TaaI ACNGT 2 cut(s) 235, 337
TaiI ACGT 2 cut(s) 219, 226
TasI AATT 1 cut(s) 280
TatI WGTACW 1 cut(s) 122
TauI GCSGC 2 cut(s) 157, 253
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 340
TseI GCWGC 2 cut(s) 206, 302
TspRI CASTG 1 cut(s) 340
ZrmI AGTACT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.