pycom08g18230

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005750
Physical Location & Seq
Reverse (-)
18493995 .. 18494544
550 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18230.1

Sequence Viewer

Length: 501 bp
ATGTGTTGGCAAAAGGCCCATTTAAACACCAAAAGCCGACACCAAGGTCCAAGACAAATAAGGATAAATGTGGTATCCTCAACGTTATCCCCGAACGAACCAACAGAGGAGCAAAGGAAGGGCAACCCAAGCGAAGCGAAGATGTCAGTCTCAGCTGTGTTGGGGTCTCAATTCGTGGCGGTGGGTGTGCCCGTGAAGAACCCATCTGCGACGGCCTCGTTTGGTGTGACCAGTTCGGCCCCATGGCGCGGCTCAACCACCGGTAACGGCGGCGGTGGTGGTGGGTTGGTGATAGAGTGCTCGTCAAGGCCACAGAAGAAGGCGACTGCGCACCACCGGAAGACTCGGCCGCGCAAAACGCAGCCGTGGGACATTAAGCGCAAGCCCACTGTGTACGCTCCCCTCCCTCCTCTCCCTGCCGAGTGGACCTTGCTCTCTAGTGCCGACGAAGCTGCTTCTGCTTCTGCATCTTCTCCGGAAGAGTCCTCCTCTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5323

Protein Analysis

167

Amino Acids

17.6

Weight (kDa)

10.01

Isoelectric Point (pI)

63.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5323 PF17257 92 - 150 1.3e-38 Family of unknown function (DUF5323)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 45
Acc16I TGCGCA 1 cut(s) 330
AccII CGCG 2 cut(s) 249, 352
AccIII TCCGGA 1 cut(s) 475
AciI CCGC 5 cut(s) 179, 249, 270, 273, 350
AclI AACGTT 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 347
AfaI GTAC 1 cut(s) 395
AfiI CCNNNNNNNGG 1 cut(s) 248
AgeI ACCGGT 1 cut(s) 260
AluBI AGCT 2 cut(s) 155, 452
AluI AGCT 2 cut(s) 155, 452
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 2 cut(s) 154, 171
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 5 cut(s) 15, 213, 237, 308, 347
ApeKI GCWGC 2 cut(s) 361, 452
AsiGI ACCGGT 1 cut(s) 260
AspLEI GCGC 4 cut(s) 249, 331, 354, 381
AspS9I GGNCC 4 cut(s) 16, 47, 238, 426
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 2 cut(s) 47, 426
BaeGI GKGCMC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 373, 439
BccI CCATC 1 cut(s) 211
BceAI ACGGC 3 cut(s) 228, 283, 349
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciVI GTATCC 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 154, 171
BfaI CTAG 1 cut(s) 438
BfuI GTATCC 1 cut(s) 85
BisI GCNGC 5 cut(s) 250, 271, 350, 362, 453
BlsI GCNGC 5 cut(s) 251, 272, 351, 363, 454
Bme18I GGWCC 2 cut(s) 47, 426
BmgT120I GGNCC 4 cut(s) 16, 47, 238, 426
BmiI GGNNCC 1 cut(s) 240
BmsI GCATC 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 347
BplI GAGNNNNNCTC 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 43, 242, 365
BsaWI WCCGGW 3 cut(s) 260, 336, 475
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 231
BseAI TCCGGA 1 cut(s) 475
BseDI CCNNGG 3 cut(s) 43, 242, 365
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 2 cut(s) 165, 486
BseNI ACTGG 1 cut(s) 231
BseRI GAGGAG 3 cut(s) 122, 399, 478
BseSI GKGCMC 1 cut(s) 192
BseX3I CGGCCG 1 cut(s) 347
BseXI GCAGC 2 cut(s) 373, 439
Bsh1236I CGCG 2 cut(s) 249, 352
Bsh1285I CGRYCG 1 cut(s) 350
BshFI GGCC 5 cut(s) 17, 215, 239, 310, 349
BshTI ACCGGT 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 350
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 3 cut(s) 261, 337, 476
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 154, 171
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 5 cut(s) 17, 215, 239, 310, 349
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 192, 302
Bsp13I TCCGGA 1 cut(s) 475
Bsp19I CCATGG 1 cut(s) 242
BspACI CCGC 5 cut(s) 179, 249, 270, 273, 350
BspANI GGCC 5 cut(s) 17, 215, 239, 310, 349
BspCNI CTCAG 2 cut(s) 164, 487
BspEI TCCGGA 1 cut(s) 475
BspFNI CGCG 2 cut(s) 249, 352
BspLI GGNNCC 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 3 cut(s) 43, 242, 365
BssT1I CCWWGG 2 cut(s) 43, 242
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 2 cut(s) 151, 495
BstDSI CCRYGG 2 cut(s) 242, 365
BstFNI CGCG 2 cut(s) 249, 352
BstHHI GCGC 4 cut(s) 249, 331, 354, 381
BstMAI GTCTC 2 cut(s) 154, 171
BstMCI CGRYCG 1 cut(s) 350
BstMWI GCNNNNNNNGC 4 cut(s) 129, 358, 449, 458
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 2 cut(s) 249, 352
BstV1I GCAGC 2 cut(s) 373, 439
BstV2I GAAGAC 1 cut(s) 347
BstZI CGGCCG 1 cut(s) 347
BsuI GTATCC 1 cut(s) 85
BsuRI GGCC 5 cut(s) 17, 215, 239, 310, 349
BtgI CCRYGG 2 cut(s) 242, 365
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 383
CfoI GCGC 4 cut(s) 249, 331, 354, 381
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 4 cut(s) 16, 47, 238, 426
Csp6I GTAC 1 cut(s) 394
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 1 cut(s) 243
CviQI GTAC 1 cut(s) 394
DdeI CTNAG 2 cut(s) 151, 495
DraI TTTAAA 1 cut(s) 24
DrdI GACNNNNNNGTC 1 cut(s) 45
DseDI GACNNNNNNGTC 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 347
EagI CGGCCG 1 cut(s) 347
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EclXI CGGCCG 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 43, 242
Eco31I GGTCTC 1 cut(s) 171
Eco47I GGWCC 2 cut(s) 47, 426
Eco52I CGGCCG 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 43, 242
ErhI CCWWGG 2 cut(s) 43, 242
FaeI CATG 1 cut(s) 246
FaiI YATR 1 cut(s) 244
FaqI GGGAC 1 cut(s) 383
FatI CATG 1 cut(s) 242
Fnu4HI GCNGC 5 cut(s) 250, 271, 350, 362, 453
Fsp4HI GCNGC 5 cut(s) 250, 271, 350, 362, 453
FspBI CTAG 1 cut(s) 438
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 4 cut(s) 248, 330, 353, 380
GluI GCNGC 5 cut(s) 250, 271, 350, 362, 453
HaeIII GGCC 5 cut(s) 17, 215, 239, 310, 349
HapII CCGG 3 cut(s) 261, 337, 476
HhaI GCGC 4 cut(s) 249, 331, 354, 381
Hin1II CATG 1 cut(s) 246
Hin6I GCGC 4 cut(s) 247, 329, 352, 379
HinP1I GCGC 4 cut(s) 247, 329, 352, 379
HinfI GANTC 2 cut(s) 343, 482
HpaII CCGG 3 cut(s) 261, 337, 476
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 394, 426
Hpy188III TCNNGA 1 cut(s) 476
Hpy8I GTNNAC 2 cut(s) 394, 426
Hpy99I CGWCG 2 cut(s) 214, 449
HpyAV CCTTC 2 cut(s) 112, 313
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 1 cut(s) 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 129, 358, 449, 458
HpyF3I CTNAG 2 cut(s) 151, 495
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 1 cut(s) 246
HspAI GCGC 4 cut(s) 247, 329, 352, 379
Kpn2I TCCGGA 1 cut(s) 475
LmnI GCTCC 2 cut(s) 109, 403
LpnPI CCDG 5 cut(s) 244, 274, 350, 429, 489
Lsp1109I GCAGC 2 cut(s) 373, 439
LweI GCATC 1 cut(s) 476
MaeI CTAG 1 cut(s) 438
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 226, 263
MboII GAAGA 6 cut(s) 151, 208, 328, 352, 462, 491
MhlI GDGCHC 2 cut(s) 192, 302
MluCI AATT 1 cut(s) 170
MlyI GAGTC 2 cut(s) 337, 491
MnlI CCTC 8 cut(s) 88, 100, 226, 413, 417, 420, 496, 499
MroI TCCGGA 1 cut(s) 475
MseI TTAA 2 cut(s) 23, 375
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 261, 337, 476
MvnI CGCG 2 cut(s) 249, 352
MwoI GCNNNNNNNGC 4 cut(s) 129, 358, 449, 458
NcoI CCATGG 1 cut(s) 242
NlaIII CATG 1 cut(s) 246
NlaIV GGNNCC 1 cut(s) 240
NmeAIII GCCGAG 2 cut(s) 325, 445
NmuCI GTSAC 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 5 cut(s) 251, 272, 351, 363, 454
PleI GAGTC 2 cut(s) 337, 490
PpsI GAGTC 2 cut(s) 337, 490
Psp1406I AACGTT 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 4 cut(s) 16, 47, 238, 426
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
SaqAI TTAA 2 cut(s) 23, 375
SatI GCNGC 5 cut(s) 250, 271, 350, 362, 453
Sau96I GGNCC 4 cut(s) 16, 47, 238, 426
SchI GAGTC 2 cut(s) 337, 491
SduI GDGCHC 2 cut(s) 192, 302
SetI ASST 5 cut(s) 49, 86, 157, 431, 454
SfaNI GCATC 1 cut(s) 476
SinI GGWCC 2 cut(s) 47, 426
Sse9I AATT 1 cut(s) 170
SsiI CCGC 5 cut(s) 179, 249, 270, 273, 350
SspMI CTAG 1 cut(s) 438
StyI CCWWGG 2 cut(s) 43, 242
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 86
TasI AATT 1 cut(s) 170
TauI GCSGC 3 cut(s) 252, 273, 352
Tru1I TTAA 2 cut(s) 23, 375
Tru9I TTAA 2 cut(s) 23, 375
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 361, 452
Tsp45I GTSAC 1 cut(s) 226
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 47, 426
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.