Rroxscaffold_3G00228720

50S ribosomal protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005750
Physical Location & Seq
Reverse (-)
13131514 .. 13131861
348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228720.1

Sequence Viewer

Length: 348 bp
ATGTCAGTCTCCGCTGTGATGTTGGGGCCTCAGCTCCTCTCCATTCCCGTCCCTCTCCGAAACCCCTCCGCGACGGCGTCGTTCGGAGTCACCCAGATGGCTCCAGCACTCTGCGGTGGTTCCGGTGGTGGGTTGGTGATAGAATGCTCGTCGAGGCCGCAGAAGAAGGCGACGGCGCACCACCGCAAGACTCGGCCACGCAAAACGGCTGCGTGGGACGTGAAACGCAAGCCGACTGTGTACGCTCCTCTGCCGCCTCTCCCCGCCGATTGGGTTTTCCTCGCTTCTTCTGAGGATGCTTCAGCTGCAGACGCTTCTTCTCCGCAAGCACCGCCCACTGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5323

Protein Analysis

115

Amino Acids

11.94

Weight (kDa)

9.92

Isoelectric Point (pI)

68.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5323 PF17257 41 - 100 2.3e-37 Family of unknown function (DUF5323)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
AcoI YGGCCR 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 129, 270
AjiI CACGTC 1 cut(s) 220
AluBI AGCT 2 cut(s) 34, 305
AluI AGCT 2 cut(s) 34, 305
Alw26I GTCTC 1 cut(s) 13
AoxI GGCC 3 cut(s) 26, 155, 194
ApeKI GCWGC 2 cut(s) 209, 305
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 82, 148
BbvCI CCTCAGC 1 cut(s) 30
BbvI GCAGC 2 cut(s) 196, 292
BccI CCATC 1 cut(s) 91
BceAI ACGGC 3 cut(s) 90, 189, 222
BcoDI GTCTC 1 cut(s) 13
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 4 cut(s) 158, 210, 254, 306
BlsI GCNGC 4 cut(s) 159, 211, 255, 307
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 27, 102, 121
BmsI GCATC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 270
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 129, 270
BseMII CTCAG 2 cut(s) 44, 282
BseRI GAGGAG 2 cut(s) 26, 237
BseXI GCAGC 2 cut(s) 196, 292
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 28, 157, 196
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 2 cut(s) 35, 230
BslI CCNNNNNNNGG 2 cut(s) 129, 270
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 2 cut(s) 35, 230
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 28, 157, 196
BspACI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
BspANI GGCC 3 cut(s) 28, 157, 196
BspCNI CTCAG 2 cut(s) 43, 283
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 27, 102, 121
BspMAI CTGCAG 1 cut(s) 310
BssNI GRCGYC 1 cut(s) 77
Bst4CI ACNGT 2 cut(s) 238, 340
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 230, 327
BstDEI CTNAG 2 cut(s) 30, 291
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 13
BstMWI GCNNNNNNNGC 3 cut(s) 305, 311, 331
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 196, 292
BsuRI GGCC 3 cut(s) 28, 157, 196
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 230, 327
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 2 cut(s) 66, 320
Csp6I GTAC 1 cut(s) 241
CviJI RGCY 8 cut(s) 28, 34, 101, 157, 196, 209, 232, 305
CviKI_1 RGCY 8 cut(s) 28, 34, 101, 157, 196, 209, 232, 305
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 30, 291
EaeI YGGCCR 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 26
FaqI GGGAC 2 cut(s) 35, 230
FauI CCCGC 1 cut(s) 271
Fnu4HI GCNGC 4 cut(s) 158, 210, 254, 306
FokI GGATG 1 cut(s) 308
Fsp4HI GCNGC 4 cut(s) 158, 210, 254, 306
GlaI GCGC 1 cut(s) 177
GluI GCNGC 4 cut(s) 158, 210, 254, 306
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 3 cut(s) 28, 157, 196
HapII CCGG 1 cut(s) 123
HgaI GACGC 2 cut(s) 66, 320
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 77
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 2 cut(s) 87, 190
HpaII CCGG 1 cut(s) 123
HphI GGTGA 2 cut(s) 82, 148
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 59, 86, 292
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 4 cut(s) 76, 82, 154, 175
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 238, 340
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 311, 331
HpyF3I CTNAG 2 cut(s) 30, 291
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 77
HspAI GCGC 1 cut(s) 176
LmnI GCTCC 3 cut(s) 39, 106, 250
LpnPI CCDG 3 cut(s) 107, 117, 136
Lsp1109I GCAGC 2 cut(s) 196, 292
LweI GCATC 1 cut(s) 286
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 88
MboII GAAGA 3 cut(s) 175, 279, 309
MlyI GAGTC 2 cut(s) 96, 184
MnlI CCTC 9 cut(s) 39, 47, 63, 76, 147, 258, 267, 286, 290
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 14, 305
MspI CCGG 1 cut(s) 123
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 3 cut(s) 305, 311, 331
NlaIV GGNNCC 3 cut(s) 27, 102, 121
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 88
PctI GAATGC 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 76
PkrI GCNGC 4 cut(s) 159, 211, 255, 307
PleI GAGTC 2 cut(s) 95, 184
PpsI GAGTC 2 cut(s) 95, 184
PspN4I GGNNCC 3 cut(s) 27, 102, 121
PspPI GGNCC 1 cut(s) 26
PstI CTGCAG 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 95
SatI GCNGC 4 cut(s) 158, 210, 254, 306
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 2 cut(s) 96, 184
SetI ASST 3 cut(s) 36, 222, 307
SfaNI GCATC 1 cut(s) 286
SfcI CTRYAG 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 95
SsiI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
TaaI ACNGT 2 cut(s) 238, 340
TaiI ACGT 1 cut(s) 222
TaqI TCGA 1 cut(s) 152
TauI GCSGC 2 cut(s) 160, 256
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 209, 305
Tsp45I GTSAC 1 cut(s) 88
TspRI CASTG 1 cut(s) 343
Tth111I GACNNNGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.