MD08G1211600.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005750
Physical Location & Seq
Reverse (-)
27518469 .. 27518828
360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1211600.v1.1.491

Sequence Viewer

Length: 360 bp
ATGTCAGTCTCGGCTGTGTTGGGGTCTCAATTCGTGGCGGTGGGTGTGCCCGTGAAGAACCCATCTGCGACGGCGTCGTTTGGTGTGACCAGTTCGGCCCCATGGCGCGGCTCAACCACCGGTAACGGCGGCGGTGGTGATGGGTTCGTGATAGAGTGCTCGTCAAGGCCACAGAAGAAGGCGACTGCGCACCACCGGAAGACTCGGCCGCGCAAAACGCAGCCGTGGGACGTTAAGCGCAAGCCCACTGTGTACGCCCCCCTCCCTCCTCTCCCTGCCGAGTGGACCTTGCTCTCTAGTGCCGACGAAGCTGCTTCTGCTTCTGCATCTTCTCCGGAAGAGTCCTCCTCTACTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5323

Protein Analysis

120

Amino Acids

12.32

Weight (kDa)

9.58

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5323 PF17257 46 - 103 1.9e-37 Family of unknown function (DUF5323)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 189
AccII CGCG 2 cut(s) 108, 211
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 5 cut(s) 38, 108, 129, 132, 209
AcoI YGGCCR 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 74
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 1 cut(s) 107
AgeI ACCGGT 1 cut(s) 119
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 2 cut(s) 13, 30
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 3 cut(s) 96, 167, 206
ApeKI GCWGC 2 cut(s) 220, 311
AsiGI ACCGGT 1 cut(s) 119
AspLEI GCGC 4 cut(s) 108, 190, 213, 240
AspS9I GGNCC 2 cut(s) 97, 285
AsuHPI GGTGA 1 cut(s) 149
AvaII GGWCC 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 161
BbvI GCAGC 2 cut(s) 232, 298
BccI CCATC 2 cut(s) 70, 134
BceAI ACGGC 3 cut(s) 87, 142, 208
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BcoDI GTCTC 2 cut(s) 13, 30
BfaI CTAG 1 cut(s) 297
BisI GCNGC 5 cut(s) 109, 130, 209, 221, 312
BlsI GCNGC 5 cut(s) 110, 131, 210, 222, 313
Bme18I GGWCC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 97, 285
BmiI GGNNCC 1 cut(s) 99
BmsI GCATC 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 206
BplI GAGNNNNNCTC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 101, 224
BsaWI WCCGGW 3 cut(s) 119, 195, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 107
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 90
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 101, 224
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 1 cut(s) 90
BseRI GAGGAG 2 cut(s) 258, 337
BseSI GKGCMC 1 cut(s) 51
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 2 cut(s) 232, 298
Bsh1236I CGCG 2 cut(s) 108, 211
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 3 cut(s) 98, 169, 208
BshTI ACCGGT 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 3 cut(s) 120, 196, 335
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 13, 30
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 3 cut(s) 98, 169, 208
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 51, 161
Bsp13I TCCGGA 1 cut(s) 334
Bsp19I CCATGG 1 cut(s) 101
BspACI CCGC 5 cut(s) 38, 108, 129, 132, 209
BspANI GGCC 3 cut(s) 98, 169, 208
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 334
BspFNI CGCG 2 cut(s) 108, 211
BspLI GGNNCC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 1 cut(s) 90
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 101, 224
BssNI GRCGYC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 101
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 1 cut(s) 354
BstDSI CCRYGG 2 cut(s) 101, 224
BstFNI CGCG 2 cut(s) 108, 211
BstHHI GCGC 4 cut(s) 108, 190, 213, 240
BstMAI GTCTC 2 cut(s) 13, 30
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 217, 308, 317
BstSLI GKGCMC 1 cut(s) 51
BstUI CGCG 2 cut(s) 108, 211
BstV1I GCAGC 2 cut(s) 232, 298
BstV2I GAAGAC 1 cut(s) 206
BstZI CGGCCG 1 cut(s) 206
BsuRI GGCC 3 cut(s) 98, 169, 208
BtgI CCRYGG 2 cut(s) 101, 224
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 242
CfoI GCGC 4 cut(s) 108, 190, 213, 240
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 97, 285
CseI GACGC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 253
CspAI ACCGGT 1 cut(s) 119
CviAII CATG 1 cut(s) 102
CviJI RGCY 8 cut(s) 14, 98, 111, 169, 208, 223, 244, 311
CviKI_1 RGCY 8 cut(s) 14, 98, 111, 169, 208, 223, 244, 311
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 206
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
EclXI CGGCCG 1 cut(s) 206
Eco130I CCWWGG 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 285
Eco52I CGGCCG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 1 cut(s) 105
FaiI YATR 1 cut(s) 103
FaqI GGGAC 1 cut(s) 242
FatI CATG 1 cut(s) 101
Fnu4HI GCNGC 5 cut(s) 109, 130, 209, 221, 312
Fsp4HI GCNGC 5 cut(s) 109, 130, 209, 221, 312
FspBI CTAG 1 cut(s) 297
FspI TGCGCA 1 cut(s) 189
GlaI GCGC 4 cut(s) 107, 189, 212, 239
GluI GCNGC 5 cut(s) 109, 130, 209, 221, 312
HaeIII GGCC 3 cut(s) 98, 169, 208
HapII CCGG 3 cut(s) 120, 196, 335
HgaI GACGC 1 cut(s) 63
HhaI GCGC 4 cut(s) 108, 190, 213, 240
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 1 cut(s) 105
Hin6I GCGC 4 cut(s) 106, 188, 211, 238
HinP1I GCGC 4 cut(s) 106, 188, 211, 238
HinfI GANTC 2 cut(s) 202, 341
HpaII CCGG 3 cut(s) 120, 196, 335
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 2 cut(s) 253, 285
Hpy188III TCNNGA 2 cut(s) 148, 335
Hpy8I GTNNAC 2 cut(s) 253, 285
Hpy99I CGWCG 3 cut(s) 73, 79, 308
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 308, 317
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 1 cut(s) 105
HspAI GCGC 4 cut(s) 106, 188, 211, 238
Kpn2I TCCGGA 1 cut(s) 334
LpnPI CCDG 5 cut(s) 103, 133, 209, 288, 348
Lsp1109I GCAGC 2 cut(s) 232, 298
LweI GCATC 1 cut(s) 335
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 85, 122
MboII GAAGA 5 cut(s) 67, 187, 211, 321, 350
MhlI GDGCHC 2 cut(s) 51, 161
MluCI AATT 1 cut(s) 29
MlyI GAGTC 2 cut(s) 196, 350
MnlI CCTC 5 cut(s) 272, 276, 279, 355, 358
MroI TCCGGA 1 cut(s) 334
MseI TTAA 1 cut(s) 234
MspI CCGG 3 cut(s) 120, 196, 335
MvnI CGCG 2 cut(s) 108, 211
MwoI GCNNNNNNNGC 3 cut(s) 217, 308, 317
NcoI CCATGG 1 cut(s) 101
NlaIII CATG 1 cut(s) 105
NlaIV GGNNCC 1 cut(s) 99
NmeAIII GCCGAG 2 cut(s) 184, 304
NmuCI GTSAC 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 189
PflFI GACNNNGTC 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 5 cut(s) 110, 131, 210, 222, 313
PleI GAGTC 2 cut(s) 196, 349
PpsI GAGTC 2 cut(s) 196, 349
PspN4I GGNNCC 1 cut(s) 99
PspPI GGNCC 2 cut(s) 97, 285
PsyI GACNNNGTC 1 cut(s) 73
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 5 cut(s) 109, 130, 209, 221, 312
Sau96I GGNCC 2 cut(s) 97, 285
SchI GAGTC 2 cut(s) 196, 350
SduI GDGCHC 2 cut(s) 51, 161
SetI ASST 3 cut(s) 234, 290, 313
SfaNI GCATC 1 cut(s) 335
SinI GGWCC 1 cut(s) 285
Sse9I AATT 1 cut(s) 29
SsiI CCGC 5 cut(s) 38, 108, 129, 132, 209
SspMI CTAG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 234
TasI AATT 1 cut(s) 29
TauI GCSGC 3 cut(s) 111, 132, 211
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 2 cut(s) 220, 311
Tsp45I GTSAC 1 cut(s) 85
TspRI CASTG 1 cut(s) 253
Tth111I GACNNNGTC 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 285
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.