Rw7G035110

50S ribosomal protein 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005750
Physical Location & Seq
Forward (+)
52257363 .. 52258225
863 bp
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UTR
Exon/CDS
Intron
Rw7G035110.1

Sequence Viewer

Length: 348 bp
ATGTCAGTCTCCGCTGTGATGTTGGGGCCTCAACTCCTCTCCATTCCCGTCCCTCTCCGAAACCCCTCCGCGACGGCGTCGTTCGGACTCACCCAGATGGCTCCACCACTCTGCGGTGGTTCCGGTGGTGGGTTGGTGATAGAATGCTCGTCGAGGCCGCAGAAGAAGGCGACGGCGCACCACCGCAAGACCCGGCCACGCAAAACGGCTGCGTGGGACGTGAAACGCAAGCCCACTGTGTACGCTCCTCTGCCGCCTCTCCCCGCCGATTGGGTTTTCCTCGCTTCTTCTGACGATGCTTCAGCTGCTGACTCTTCTTCTCCGCAAGCACCGCCCACTGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF5323

Protein Analysis

115

Amino Acids

11.98

Weight (kDa)

9.92

Isoelectric Point (pI)

69.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF5323 PF17257 41 - 100 2.1e-37 Family of unknown function (DUF5323)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
AcoI YGGCCR 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 3 cut(s) 113, 129, 270
AjiI CACGTC 1 cut(s) 220
AluBI AGCT 1 cut(s) 305
AluI AGCT 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 13
AlwNI CAGNNNCTG 1 cut(s) 308
AoxI GGCC 3 cut(s) 26, 155, 194
ApeKI GCWGC 2 cut(s) 209, 305
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 82, 148
BbvI GCAGC 2 cut(s) 196, 292
BccI CCATC 1 cut(s) 91
BceAI ACGGC 3 cut(s) 90, 189, 222
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 13
BisI GCNGC 4 cut(s) 158, 210, 254, 306
BlsI GCNGC 4 cut(s) 159, 211, 255, 307
Bme1390I CCNGG 1 cut(s) 193
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 27, 102, 121
BmrFI CCNGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 129, 270
BseLI CCNNNNNNNGG 3 cut(s) 113, 129, 270
BseRI GAGGAG 2 cut(s) 26, 237
BseXI GCAGC 2 cut(s) 196, 292
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 28, 157, 196
BsiSI CCGG 2 cut(s) 123, 193
BslFI GGGAC 2 cut(s) 35, 230
BslI CCNNNNNNNGG 3 cut(s) 113, 129, 270
BsmAI GTCTC 1 cut(s) 13
BsmFI GGGAC 2 cut(s) 35, 230
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 28, 157, 196
BspACI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
BspANI GGCC 3 cut(s) 28, 157, 196
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 27, 102, 121
BssNI GRCGYC 1 cut(s) 77
Bst4CI ACNGT 2 cut(s) 238, 340
Bst6I CTCTTC 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 230, 327
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 178
BstMAI GTCTC 1 cut(s) 13
BstMWI GCNNNNNNNGC 2 cut(s) 305, 331
BstSCI CCNGG 1 cut(s) 191
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 196, 292
BsuRI GGCC 3 cut(s) 28, 157, 196
BtrI CACGTC 1 cut(s) 220
BtsIMutI CAGTG 2 cut(s) 234, 336
Cac8I GCNNGC 2 cut(s) 230, 327
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 26
CseI GACGC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 241
CviJI RGCY 7 cut(s) 28, 101, 157, 196, 209, 232, 305
CviKI_1 RGCY 7 cut(s) 28, 101, 157, 196, 209, 232, 305
CviQI GTAC 1 cut(s) 241
EaeI YGGCCR 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 26
FaqI GGGAC 2 cut(s) 35, 230
FauI CCCGC 1 cut(s) 271
Fnu4HI GCNGC 4 cut(s) 158, 210, 254, 306
Fsp4HI GCNGC 4 cut(s) 158, 210, 254, 306
GlaI GCGC 1 cut(s) 177
GluI GCNGC 4 cut(s) 158, 210, 254, 306
HaeIII GGCC 3 cut(s) 28, 157, 196
HapII CCGG 2 cut(s) 123, 193
HgaI GACGC 1 cut(s) 66
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 77
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 2 cut(s) 87, 311
HpaII CCGG 2 cut(s) 123, 193
HphI GGTGA 2 cut(s) 82, 148
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 59, 86, 292
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 4 cut(s) 76, 82, 154, 175
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 2 cut(s) 238, 340
HpyCH4IV ACGT 1 cut(s) 219
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 331
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 77
HspAI GCGC 1 cut(s) 176
LmnI GCTCC 2 cut(s) 106, 250
LpnPI CCDG 3 cut(s) 107, 136, 206
Lsp1109I GCAGC 2 cut(s) 196, 292
LweI GCATC 1 cut(s) 286
MaeII ACGT 1 cut(s) 219
MboII GAAGA 4 cut(s) 175, 279, 306, 309
MlyI GAGTC 2 cut(s) 81, 305
MnlI CCTC 8 cut(s) 39, 47, 63, 76, 147, 258, 267, 290
MslI CAYNNNNRTG 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 14, 305
MspI CCGG 2 cut(s) 123, 193
MspR9I CCNGG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 305, 331
NciI CCSGG 1 cut(s) 193
NlaIV GGNNCC 3 cut(s) 27, 102, 121
PctI GAATGC 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 76
PkrI GCNGC 4 cut(s) 159, 211, 255, 307
PleI GAGTC 2 cut(s) 81, 305
PpsI GAGTC 2 cut(s) 81, 305
PspN4I GGNNCC 3 cut(s) 27, 102, 121
PspPI GGNCC 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 308
PsyI GACNNNGTC 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 95
SatI GCNGC 4 cut(s) 158, 210, 254, 306
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 2 cut(s) 81, 305
ScrFI CCNGG 1 cut(s) 193
SetI ASST 2 cut(s) 222, 307
SfaNI GCATC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 95
SsiI CCGC 9 cut(s) 12, 69, 114, 158, 184, 254, 264, 323, 332
StyD4I CCNGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 238, 340
TaiI ACGT 1 cut(s) 222
TaqI TCGA 1 cut(s) 152
TauI GCSGC 2 cut(s) 160, 256
TscAI CASTG 2 cut(s) 241, 343
TseI GCWGC 2 cut(s) 209, 305
TspRI CASTG 2 cut(s) 241, 343
Tth111I GACNNNGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.