FvH4_5g33450

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013894
Physical Location & Seq
Reverse (-)
24177956 .. 24183226
5271 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33450.t1

Sequence Viewer

Length: 1035 bp
ATGAGGGAGGAAATGAGGGGCCAATTGGTTTACGGTGAGAAAAGAGAGGAGGAGGAGGTGGAGGTGGAGCAGCCGCAACGGTGTCGTATTGTTAACAAGAGGTTTGTGGTGGTGGGTTATGCTCTTACGTCTAAGAAGACCAAGAGCTTCTTGCAGCCCAAGCTCGAAGGTTTAGCTAGAAACAAGGGGATATCGTTTGTTGCTATTGATCTTAGCAGGCCTCTTTCCGATCAAGGACCTTTTGACATTGTCTTGCATAAGCTTTCAGGAAGAGAGTGGCGCCAGATTCTTGAGGACTATAGGAAAACACATCCAGAAGTTACTGTTCTTGATCCTCCTGATGCTATACAACCTTTACACAATCGTCAGTCCATGCTCCAGGCTGTTGCTGATATGGACTTGTCTGATTCCTATGGCAAAGTTGGTGTTCCCAGACAATTGGTTATCAAGAGGGATGCATCATCCATCCCTGACGCTGTTAGAGATAGTGGGCTGAAACTTCCTCTTGTTGCTAAGCCATTGGTGAATGATGGAAGCGTAAAATCCCATCAACTATCACTTGCCTATGATCGATACTCCCTCCAAAAGCTTGAACCTCCCCTTGTTCTTCAGGAATTCGTAAACCATGGAGGAGTTCTTTTTAAAGTATATATCGTTGGTGAAGCCATAAAAGTGGTCAGACGTTTTTCTTTACCCGATGTCAGTAAAAGAGAGCTGTCTGAAACTGCGGGTGTATATCGTTTTCCACGGGTTTCATGTGCTGCAGCTTCAGCAGATGATGCAGATCTGGACCCTAGTATTGCTGAACTTCCTCCACGGCCATTACTAGAGAGGCTTGCCAAGGAACTTCGTCGTCGGCTGGGTCTGCGGTTATTCAACTTGGACATGATTCGAGAACATGGAACCAGAGATCGGTTTTATGTCATTGACATTAACTACTTCCCTGGGTATGGGAAAATGCCGGAGTATGAGCACATATTTACGGATTTCCTATTGAGCCTGGCAGAGGCCAAGTACAAAAAGAGATCTGGCTGA

Protein Analysis

345

Amino Acids

39.2

Weight (kDa)

9.08

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ins134_P3_kin_N PF17927 37 - 116 3.1e-38 Inositol 1,3,4-trisphosphate 5/6-kinase pre-ATP-grasp domain
Ins134_P3_kin PF05770 136 - 332 4.8e-94 Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08170 AT4G08170
fragaria_vesca FvH4_5g33450 FvH4_5g33450
malus_domestica MD08G1211500.v1.1
prunus_persica Prupe.1G543400_v2.0.a1 Prupe.1G543400_v2.0.a1
pyrus_communis pycom08g18220
rosa_chinensis RchiOBHm_Chr7g0232171
rosa_laevigata RLG00000001380 RLG00000001383
rosa_roxburghii Rroxscaffold_3G00228700
rosa_rugosa Rorug07G0270100
rosa_samantha Rh7BG398700 Rh7CG445000
rosa_wichuraiana Rw7G035120 Rw7G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 279
AciI CCGC 3 cut(s) 74, 728, 868
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 2 cut(s) 593, 753
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 1 cut(s) 1016
AfiI CCNNNNNNNGG 3 cut(s) 912, 950, 1006
AgsI TTSAA 2 cut(s) 593, 877
AjnI CCWGG 3 cut(s) 378, 943, 999
AluBI AGCT 7 cut(s) 147, 163, 176, 262, 589, 715, 767
AluI AGCT 7 cut(s) 147, 163, 176, 262, 589, 715, 767
Alw21I GWGCWC 1 cut(s) 975
AlwI GGATC 1 cut(s) 326
AoxI GGCC 4 cut(s) 19, 218, 818, 1008
ApeKI GCWGC 4 cut(s) 70, 154, 761, 764
ApoI RAATTY 1 cut(s) 614
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 3 cut(s) 19, 236, 790
AsuHPI GGTGA 3 cut(s) 47, 535, 671
AvaII GGWCC 2 cut(s) 236, 790
BanI GGYRCC 1 cut(s) 279
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 4 cut(s) 82, 166, 748, 776
BccI CCATC 3 cut(s) 473, 524, 555
BceAI ACGGC 1 cut(s) 833
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 3 cut(s) 380, 945, 1001
BfaI CTAG 3 cut(s) 177, 795, 827
BfmI CTRYAG 2 cut(s) 298, 762
BfoI RGCGCY 1 cut(s) 283
BglII AGATCT 2 cut(s) 784, 1025
BisI GCNGC 5 cut(s) 71, 74, 155, 762, 765
BlpI GCTNAGC 1 cut(s) 513
BlsI GCNGC 5 cut(s) 72, 75, 156, 763, 766
Bme1390I CCNGG 3 cut(s) 380, 945, 1001
Bme18I GGWCC 2 cut(s) 236, 790
BmgT120I GGNCC 3 cut(s) 19, 236, 790
BmiI GGNNCC 4 cut(s) 20, 281, 792, 904
BmrFI CCNGG 3 cut(s) 380, 945, 1001
BmsI GCATC 4 cut(s) 331, 445, 467, 769
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 311
Bsa29I ATCGAT 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 6 cut(s) 625, 746, 815, 840, 943, 944
Bsc4I CCNNNNNNNGG 3 cut(s) 912, 950, 1006
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 3 cut(s) 380, 945, 1001
BseCI ATCGAT 1 cut(s) 571
BseDI CCNNGG 6 cut(s) 625, 746, 815, 840, 943, 944
BseGI GGATG 4 cut(s) 310, 460, 461, 465
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 912, 950, 1006
BseRI GAGGAG 4 cut(s) 62, 65, 68, 645
BseXI GCAGC 4 cut(s) 82, 166, 748, 776
BseYI CCCAGC 1 cut(s) 859
BshFI GGCC 4 cut(s) 21, 220, 820, 1010
BshNI GGYRCC 1 cut(s) 279
BshVI ATCGAT 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 975
BsiSI CCGG 1 cut(s) 962
BslI CCNNNNNNNGG 3 cut(s) 912, 950, 1006
BsnI GGCC 4 cut(s) 21, 220, 820, 1010
Bsp1286I GDGCHC 1 cut(s) 975
Bsp143I GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
Bsp1720I GCTNAGC 1 cut(s) 513
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 3 cut(s) 74, 728, 868
BspANI GGCC 4 cut(s) 21, 220, 820, 1010
BspDI ATCGAT 1 cut(s) 571
BspLI GGNNCC 4 cut(s) 20, 281, 792, 904
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 279
BssECI CCNNGG 6 cut(s) 625, 746, 815, 840, 943, 944
BssMI GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
BssNI GRCGYC 1 cut(s) 280
BssT1I CCWWGG 2 cut(s) 625, 840
Bst2UI CCWGG 3 cut(s) 380, 945, 1001
Bst4CI ACNGT 3 cut(s) 35, 81, 325
Bst6I CTCTTC 1 cut(s) 265
BstACI GRCGYC 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 779
BstC8I GCNNGC 2 cut(s) 218, 837
BstDEI CTNAG 3 cut(s) 132, 212, 513
BstDSI CCRYGG 3 cut(s) 625, 746, 815
BstENI CCTNNNNNAGG 1 cut(s) 1004
BstF5I GGATG 4 cut(s) 310, 460, 461, 465
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 1 cut(s) 282
BstKTI GATC 7 cut(s) 211, 232, 334, 571, 787, 913, 1028
BstMBI GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
BstMWI GCNNNNNNNGC 4 cut(s) 160, 770, 779, 865
BstNI CCWGG 3 cut(s) 380, 945, 1001
BstSCI CCNGG 3 cut(s) 378, 943, 999
BstSFI CTRYAG 2 cut(s) 298, 762
BstV1I GCAGC 4 cut(s) 82, 166, 748, 776
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 784, 1025
BstXI CCANNNNNNTGG 2 cut(s) 439, 673
BstYI RGATCY 2 cut(s) 784, 1025
Bsu15I ATCGAT 1 cut(s) 571
BsuRI GGCC 4 cut(s) 21, 220, 820, 1010
BsuTUI ATCGAT 1 cut(s) 571
BtgI CCRYGG 3 cut(s) 625, 746, 815
BtsCI GGATG 4 cut(s) 310, 460, 461, 465
Cac8I GCNNGC 2 cut(s) 218, 837
CfoI GCGC 1 cut(s) 282
Cfr13I GGNCC 3 cut(s) 19, 236, 790
ClaI ATCGAT 1 cut(s) 571
CseI GACGC 1 cut(s) 482
Csp6I GTAC 1 cut(s) 1015
CviAII CATG 5 cut(s) 373, 626, 756, 886, 899
CviQI GTAC 1 cut(s) 1015
DdeI CTNAG 3 cut(s) 132, 212, 513
DinI GGCGCC 1 cut(s) 281
DpnI GATC 7 cut(s) 210, 231, 333, 570, 786, 912, 1027
DpnII GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
DraI TTTAAA 1 cut(s) 643
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 625, 840
Eco147I AGGCCT 1 cut(s) 220
Eco32I GATATC 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 236, 790
Eco57I CTGAAG 2 cut(s) 593, 753
EcoNI CCTNNNNNAGG 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 614
EcoRII CCWGG 3 cut(s) 378, 943, 999
EcoRV GATATC 1 cut(s) 192
EcoT14I CCWWGG 2 cut(s) 625, 840
EcoT22I ATGCAT 1 cut(s) 460
EgeI GGCGCC 1 cut(s) 281
EheI GGCGCC 1 cut(s) 281
ErhI CCWWGG 2 cut(s) 625, 840
FaeI CATG 5 cut(s) 376, 629, 759, 889, 902
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FatI CATG 5 cut(s) 372, 625, 755, 885, 898
FauI CCCGC 1 cut(s) 721
Fnu4HI GCNGC 5 cut(s) 71, 74, 155, 762, 765
FokI GGATG 4 cut(s) 297, 448, 452, 467
Fsp4HI GCNGC 5 cut(s) 71, 74, 155, 762, 765
FspBI CTAG 3 cut(s) 177, 795, 827
GlaI GCGC 1 cut(s) 281
GluI GCNGC 5 cut(s) 71, 74, 155, 762, 765
GsaI CCCAGC 1 cut(s) 863
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 4 cut(s) 21, 220, 820, 1010
HapII CCGG 1 cut(s) 962
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 282
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 5 cut(s) 376, 629, 759, 889, 902
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 2 cut(s) 260, 587
HinfI GANTC 3 cut(s) 286, 407, 889
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 1 cut(s) 962
HphI GGTGA 3 cut(s) 47, 535, 671
Hpy166II GTNNAC 3 cut(s) 31, 94, 622
Hpy188I TCNGA 4 cut(s) 229, 406, 680, 721
Hpy188III TCNNGA 9 cut(s) 267, 290, 314, 329, 338, 448, 611, 788, 893
Hpy8I GTNNAC 3 cut(s) 31, 94, 622
Hpy99I CGWCG 2 cut(s) 855, 858
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 3 cut(s) 35, 81, 325
HpyCH4IV ACGT 2 cut(s) 128, 682
HpyCH4V TGCA 5 cut(s) 154, 256, 458, 764, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 770, 779, 865
HpyF3I CTNAG 3 cut(s) 132, 212, 513
HpySE526I ACGT 2 cut(s) 128, 682
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 5 cut(s) 376, 629, 759, 889, 902
HspAI GCGC 1 cut(s) 280
KasI GGCGCC 1 cut(s) 279
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
LmnI GCTCC 2 cut(s) 67, 381
Lsp1109I GCAGC 4 cut(s) 82, 166, 748, 776
LweI GCATC 4 cut(s) 331, 445, 467, 769
MaeI CTAG 3 cut(s) 177, 795, 827
MaeII ACGT 2 cut(s) 128, 682
MaeIII GTNAC 1 cut(s) 319
MalI GATC 7 cut(s) 210, 231, 333, 570, 786, 912, 1027
MboI GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
MboII GAAGA 3 cut(s) 148, 282, 599
MfeI CAATTG 2 cut(s) 23, 437
MflI RGATCY 2 cut(s) 784, 1025
MhlI GDGCHC 1 cut(s) 975
MluCI AATT 3 cut(s) 23, 437, 614
Mly113I GGCGCC 1 cut(s) 280
Mph1103I ATGCAT 1 cut(s) 460
MseI TTAA 3 cut(s) 93, 642, 933
MslI CAYNNNNRTG 1 cut(s) 671
MspI CCGG 1 cut(s) 962
MspR9I CCNGG 3 cut(s) 380, 945, 1001
MunI CAATTG 2 cut(s) 23, 437
MvaI CCWGG 3 cut(s) 380, 945, 1001
MwoI GCNNNNNNNGC 4 cut(s) 160, 770, 779, 865
NarI GGCGCC 1 cut(s) 280
NcoI CCATGG 1 cut(s) 625
NdeII GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
NlaIII CATG 5 cut(s) 376, 629, 759, 889, 902
NlaIV GGNNCC 4 cut(s) 20, 281, 792, 904
NsiI ATGCAT 1 cut(s) 460
PasI CCCWGGG 1 cut(s) 944
PceI AGGCCT 1 cut(s) 220
PfeI GAWTC 3 cut(s) 286, 407, 889
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 5 cut(s) 72, 75, 156, 763, 766
PluTI GGCGCC 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 3 cut(s) 378, 943, 999
PspFI CCCAGC 1 cut(s) 859
PspGI CCWGG 3 cut(s) 378, 943, 999
PspN4I GGNNCC 4 cut(s) 20, 281, 792, 904
PspPI GGNCC 3 cut(s) 19, 236, 790
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 766
PsuI RGATCY 2 cut(s) 784, 1025
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 1 cut(s) 1016
RsaNI GTAC 1 cut(s) 1015
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 3 cut(s) 93, 642, 933
SatI GCNGC 5 cut(s) 71, 74, 155, 762, 765
Sau3AI GATC 7 cut(s) 208, 229, 331, 568, 784, 910, 1025
Sau96I GGNCC 3 cut(s) 19, 236, 790
ScrFI CCNGG 3 cut(s) 380, 945, 1001
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 4 cut(s) 331, 445, 467, 769
SfcI CTRYAG 2 cut(s) 298, 762
SfoI GGCGCC 1 cut(s) 281
SinI GGWCC 2 cut(s) 236, 790
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 3 cut(s) 23, 437, 614
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 3 cut(s) 74, 728, 868
SspDI GGCGCC 1 cut(s) 279
SspMI CTAG 3 cut(s) 177, 795, 827
StuI AGGCCT 1 cut(s) 220
StyD4I CCNGG 3 cut(s) 378, 943, 999
StyI CCWWGG 2 cut(s) 625, 840
TaaI ACNGT 3 cut(s) 35, 81, 325
TaiI ACGT 2 cut(s) 131, 685
TaqI TCGA 3 cut(s) 165, 571, 892
TasI AATT 3 cut(s) 23, 437, 614
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 3 cut(s) 286, 407, 889
Tru1I TTAA 3 cut(s) 93, 642, 933
Tru9I TTAA 3 cut(s) 93, 642, 933
TseI GCWGC 4 cut(s) 70, 154, 761, 764
TspDTI ATGAA 1 cut(s) 744
TspGWI ACGGA 1 cut(s) 998
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 236, 790
XagI CCTNNNNNAGG 1 cut(s) 1004
XapI RAATTY 1 cut(s) 614
XspI CTAG 3 cut(s) 177, 795, 827
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.