RLG00000001383

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0013894
Physical Location & Seq
Forward (+)
14920609 .. 14928552
7944 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001383

Sequence Viewer

Length: 3630 bp
ATGCCGGAGTATGAGCACATATTTACGGACTTCCTATTTAGCCTGGTAGAGGGAAAAAACAAGGGGATATTATTCGTTGCTATTGATCATAACAGGCCTCTTTCGGATCAAGGCCCTTTTGACATTGTCTTGCATAAGCTTTTGGGAAGAGAGTGGCGCCAGATTCTTGAGGACTATAGGAAAACACATCCAGAAGTTACTGTTCTTGATCCTCCTGATGCGATACAACCTTTACATAATCGTCAGTCCATGCTCCAGGCTGTTGCTGATATGGACTTGTCTGATTCCTATGGCAAAGTTGGTGTTCTAAGACAATTGGCTATCAAGAGAGATGCATCATCCATCCCTGATGCTGTTACAGATAGTGGGCTGAAACTACCTCTTGTTGCTAAGCCACTGGTGAATGATGGAAGTGTAAAATCCCATCAACTATCACTTGCCTATGATCGATACTCCCTCCAAAAGCTTGAACCCCCTCTCGTTCTTCAGGAGTTTGTAAACCATGGAGGTGTTCTTTTTGAAGTATATATCGTCGGTGAAGCTATAAAAGTGGTCAGACGTTTCTCTTTACCTGATGTCAGTAAAAGAGAGCTTTCTGAAACCGCGGGTGTCTATCGTTTTCTGAGGGTTTCATGTGCTGCAGCTTCAGCAGATGATGCAGATCTGGACCCTAGCATAGCTGAACTTCCTCCACGGCCATTACTCGAGAGACTTGCTAAGGAACTTCGTCGTCGACTGGGTCTGCGGTTATTCAACTTGGACATGATCCGAGAACATGGAACTAGAGATCGGTTTTATGTCATTGACATTAACTACTTCCCTGGGTATGGGAAAATGCCCGAGTATGAGCACATATTTACGGACTTCCTATTGAGCCTGGTGGAGGCGAAGTACAAAAAGAGATCTATATGTAAAATTTTCACAAGTGAACAAGCCCTTGAGAATCCAATTAGACAAGGTAGGGTTGTCAATCCATCCACAAATGTTCCAGTTTACATGTCAGAATCAAAATTTATTCATGAAATTGTTAAAGAGATTACGGCACATGTACATAAACGTACCCATTTAGATGTTGCAAAATATGCGGTTGGAATAGAGTCGCGTGTACAAGATGTGCTCAAGCTTTTAGATATTGGAGAATGTGATGTCCGCATGGTAGGAATATGGGGAACAGGTGGAATAGGAAAGACAACAATTGCTAAAGCAATATACAATTCAATTGCTCATGAGTTTGAATTTGGTTGCTTTTTGGCAAATGTTAGAGAAGAATCAATACCATATGGAGGTCTAGTAAATTTACAAAATATTCTTCTTTCCAAGATTCCAGGGGGAAAAGATGTCAAGGTAACTAATGTTGATGAAGGGATCACTTTCATAAAAGAATTGCTGAGTCATAAAAGAGTCCTCTTAATTCTTGATGATGTGAATCAATTGAATCAGTTGAACAACTTAGCTGGAGGGCATGATTGGTTCGGTTTGAAAAGTAGAGTTATTATAACATCAAGGGATATACATTTACTCTATGCTCATCACATAATTCTGACATACAAGGTTAAGAAATTATGTTTGAAAGAAGCTTGTGAGCTCTTCCTTAGTTGGAATGGTCAGTTAGCAAATAGAGAATTGGTTGATTATGAGAATTGTTCTGACCAAATTACTCGTGAATTACAGAGTGTAGTGCATTATGCTCAAGGCTTACCATTAGCTCTTAAAGTTTTAGGGTCGCTTTTATGTGGTAGAAGTATAGATGAATGGCAGGATGTATTAGATAGTTACAAAAAAGTTCCTAACAAAGAGATTCAAGAAGTTCTCAAAATCAGTTTTGAGGCTCTAGAAGATCCGGTGAAGGAAATTTTCCTCGACATTGCATGTTTCTTTAAAGGTAAAAGGACAGAGTATGTGATGCAAGCACTAGACGGTTCTGACCTCAACCCCAAGTTTGCCATTAAAGTTCTAGTAGAAAAGGCGCTCATAAACATTGATAAGAAAGAACGTATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGAAATAATTCGTCAAGAGTCACCTGGACAACCTGGTGAGCGTAGCAGATTATGGTTTCATGAAGATGTTTATGATGTTCTAACGGAAAACACTGGAACAAACAAAATTAAAGGCATAATTGTGAAATTTCCGAAGGCAAGTAAGATATCCTTGAGTGCTAAATGCTTTAAAAAGATGAAAAATCTTCAACTTTTTATAAACGTTAATGCTCGCTTCTCAGGGGAGAGTTTCCAACATATGAAATCTATGGACTTGGAAGGTTGTGAATCCTTGACAGAAGTCCCCAACCTCTCGGGATTGTTCCCAAGCTTAGAGACCCTAAGTCTATGCTATTGTACGAGTTTGGTTGAGCTGCACCCTTCTGTTGTATCCCTTGAAAAACTTGATTACATAGATCTTGAAGGATGCACTAGTCTTCAGCATTTTCCGGAAATTGAGGAGAAGGTGGAATCCTTAACAAGATTGAATCTATCCAATAGTGGCATCAAAGAGTTGCCTTCATCAATTAATAATCTCATTAGTCTTGAAGTGTTACGTGTCGAAGGTTGTGAAAGCCTTGCGCAGTTTCCACAGAGCATTTATGACTTGCAGCAGTTAAAAGTGTTCTTCTTATATGATTGTCCAAAGATTGTTATTTCTACTAATAAAATGAACTCTGGAGTGCTTCCGACTAATCCAAATGTTGCAGATGACAAATATGGTTCTATACCAGAAAGTGATGATGAAGCCAGTTTACGGCTTCCAAATCTACGAAGATTCATAGCAGGAGATTCTAATTTATCGGACACTGATTTCCTTGTTAGTCTCGATTGTGTGTCAACACTGGGAATATTAGATCTCTCTGGAAATATTTTTGAAAGTCTTCCTTGCATTACCAAATTTGTCAGTCTGGCTGAGGACGTCGAAAAGATGAAAAGTAGTTTGCTGATCAATGAGGCTACTCTCCTCTCCCTTTGTCTCTCGCCTATACAATCAACATTCCAGGTTGTATTTCCCGCAAGTGATGTTCCACAGTGGTTTAGCTGTCAAATGGATTTCACAGCAGGGTTTCCCTTTTATGAATATTCTATTGACATCCTTCCACATTTGCGATTAGAGAAAACAGGACTGGCCGTCTGTGTTGCTGTTGAAAAGCCACAATCTGATGACCGTTATGAGTGCAGCTTCAAGGTCCATTTTACTTTCAATCATCGTCTTTGGCCTGATATTGTTTTCGGAGAAATTGATACAGCAGTGTCGGCTCATGCGTTGATGTATTATTACCCCTTCACTAACTTGCGGCCACTTCTTGATTTCGTTCCCCTGCCTTTTACATTTCGAGTTACTATCGAATCTCCTGTCGCCAACGACTGGCATTACTGCTCTATCAAAAGCTGCGGGGTACATCTTGTAATGCCACCAAATGAGGACGTCTCCATAAAGATTTCCAAGGCACATATCCTCACTCTTCCACGGTCGCCGCCCAGAGGGAATCCTGACCCTGCAGACTTGGACGACGGACAAGGCTTCACATGGTCTGAAGATGATGAAGATGAGTACATGCTTTCTGAAGATGAAGATGAGTACATGCTTTCTCAAGATGATGAAGATCCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1210

Amino Acids

136.75

Weight (kDa)

5.23

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ins134_P3_kin_N PF17927 18 - 75 2e-24 Inositol 1,3,4-trisphosphate 5/6-kinase pre-ATP-grasp domain
Ins134_P3_kin PF05770 95 - 291 4.2e-89 Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp domain
NB-ARC PF00931 366 - 530 1.8e-16 NB-ARC domain
WHD_ROQ1 PF23282 616 - 684 1.1e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 786 - 883 6.2e-09 Leucine-rich repeat region
LRR_13 PF23286 803 - 970 1.8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013894)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08170 AT4G08170
fragaria_vesca FvH4_5g33450 FvH4_5g33450
malus_domestica MD08G1211500.v1.1
prunus_persica Prupe.1G543400_v2.0.a1 Prupe.1G543400_v2.0.a1
pyrus_communis pycom08g18220
rosa_chinensis RchiOBHm_Chr7g0232171
rosa_laevigata RLG00000001380 RLG00000001383
rosa_roxburghii Rroxscaffold_3G00228700
rosa_rugosa Rorug07G0270100
rosa_samantha Rh7BG398700 Rh7CG445000
rosa_wichuraiana Rw7G035120 Rw7G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1496, 2228
AatII GACGTC 2 cut(s) 2934, 3444
Acc16I TGCGCA 1 cut(s) 2592
AccB1I GGYRCC 1 cut(s) 156
AccB7I CCANNNNNTGG 1 cut(s) 3381
AccI GTMKAC 1 cut(s) 733
AccII CGCG 2 cut(s) 605, 1102
AccIII TCCGGA 1 cut(s) 2458
AciI CCGC 9 cut(s) 603, 605, 745, 1085, 1150, 3027, 3310, 3408, 3491
AclI AACGTT 1 cut(s) 2232
AclWI GGATC 6 cut(s) 114, 203, 760, 1373, 1832, 3614
AcoI YGGCCR 3 cut(s) 695, 3141, 3311
AcsI RAATTY 7 cut(s) 915, 1010, 1235, 1294, 1851, 2156, 2909
AcuI CTGAAG 5 cut(s) 470, 630, 2432, 3570, 3600
AcyI GRCGYC 3 cut(s) 157, 2931, 3441
AfaI GTAC 8 cut(s) 893, 1050, 1060, 1107, 2368, 3414, 3569, 3596
AfiI CCNNNNNNNGG 7 cut(s) 49, 827, 883, 1283, 2766, 3381, 3497
AflIII ACRYGT 3 cut(s) 996, 1045, 2566
AhdI GACNNNNNGTC 2 cut(s) 2352, 3143
AhlI ACTAGT 1 cut(s) 2441
AjnI CCWGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
Alw21I GWGCWC 4 cut(s) 18, 852, 1119, 1587
Alw26I GTCTC 5 cut(s) 703, 2339, 2840, 2993, 3448
AlwI GGATC 6 cut(s) 114, 203, 760, 1373, 1832, 3614
Ama87I CYCGRG 3 cut(s) 704, 839, 2323
Aor13HI TCCGGA 1 cut(s) 2458
AoxI GGCC 6 cut(s) 95, 112, 695, 3141, 3230, 3311
ApeKI GCWGC 6 cut(s) 638, 641, 2383, 2620, 3192, 3405
ApoI RAATTY 7 cut(s) 915, 1010, 1235, 1294, 1851, 2156, 2909
ArsI GACNNNNNNTTYG 2 cut(s) 3354, 3386
AseI ATTAAT 1 cut(s) 2538
Asp700I GAANNNNTTC 2 cut(s) 2037, 2892
AspLEI GCGC 3 cut(s) 159, 1969, 2593
AspS9I GGNCC 3 cut(s) 113, 667, 3202
AsuHPI GGTGA 5 cut(s) 412, 548, 1855, 2043, 2078
AvaI CYCGRG 3 cut(s) 704, 839, 2323
AvaII GGWCC 2 cut(s) 667, 3202
BaeI ACNNNNGTAYC 2 cut(s) 3249, 3282
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 1587
BarI GAAGNNNNNNTAC 4 cut(s) 1257, 1289, 2748, 2780
BauI CACGAG 1 cut(s) 1659
BbsI GAAGAC 2 cut(s) 2438, 2885
Bbv12I GWGCWC 4 cut(s) 18, 852, 1119, 1587
BbvCI CCTCAGC 1 cut(s) 2925
BbvI GCAGC 6 cut(s) 625, 653, 2370, 2632, 3204, 3392
BccI CCATC 4 cut(s) 350, 401, 432, 982
BceAI ACGGC 4 cut(s) 710, 1056, 2783, 3128
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BciT130I CCWGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
BciVI GTATCC 1 cut(s) 2410
BclI TGATCA 2 cut(s) 85, 2958
BcoDI GTCTC 5 cut(s) 703, 2339, 2840, 2993, 3448
BcuI ACTAGT 1 cut(s) 2441
BfaI CTAG 7 cut(s) 672, 783, 1289, 1832, 1913, 1955, 2442
BfmI CTRYAG 3 cut(s) 175, 639, 3513
BfoI RGCGCY 2 cut(s) 160, 1970
BfuI GTATCC 1 cut(s) 2410
BglII AGATCT 4 cut(s) 661, 902, 2425, 2866
BisI GCNGC 8 cut(s) 639, 642, 2384, 2621, 3193, 3311, 3406, 3491
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 8 cut(s) 640, 643, 2385, 2622, 3194, 3312, 3407, 3492
Bme1390I CCNGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
Bme18I GGWCC 2 cut(s) 667, 3202
BmeRI GACNNNNNGTC 2 cut(s) 2352, 3143
BmeT110I CYCGRG 3 cut(s) 704, 839, 2323
BmgT120I GGNCC 3 cut(s) 113, 667, 3202
BmiI GGNNCC 2 cut(s) 158, 669
BmrFI CCNGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
BmrI ACTGGG 2 cut(s) 746, 2864
BmsI GCATC 9 cut(s) 208, 322, 340, 344, 646, 1893, 1992, 2426, 2523
BmuI ACTGGG 2 cut(s) 746, 2864
BoxI GACNNNNGTC 1 cut(s) 2309
BpiI GAAGAC 2 cut(s) 2438, 2885
BplI GAGNNNNNCTC 2 cut(s) 3428, 3460
BpmI CTGGAG 3 cut(s) 239, 1476, 2709
Bpu10I CCTNAGC 2 cut(s) 717, 2925
Bpu1102I GCTNAGC 1 cut(s) 390
BpuEI CTTGAG 6 cut(s) 188, 959, 1103, 1674, 2203, 3591
Bsa29I ATCGAT 1 cut(s) 448
BsaAI YACGTR 1 cut(s) 2567
BsaBI GATNNNNATC 3 cut(s) 660, 1425, 3618
BsaHI GRCGYC 3 cut(s) 157, 2931, 3441
BsaI GGTCTC 1 cut(s) 2339
BsaJI CCNNGG 8 cut(s) 502, 603, 692, 820, 821, 1325, 3459, 3482
BsaWI WCCGGW 2 cut(s) 1840, 2458
BsaXI ACNNNNNCTCC 4 cut(s) 875, 905, 1275, 1305
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 827, 883, 1283, 2766, 3381, 3497
Bse1I ACTGG 8 cut(s) 402, 741, 989, 2128, 2760, 2859, 3144, 3386
Bse3DI GCAATG 1 cut(s) 1863
Bse8I GATNNNNATC 3 cut(s) 660, 1425, 3618
BseAI TCCGGA 1 cut(s) 2458
BseBI CCWGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
BseCI ATCGAT 1 cut(s) 448
BseDI CCNNGG 8 cut(s) 502, 603, 692, 820, 821, 1325, 3459, 3482
BseGI GGATG 8 cut(s) 187, 338, 342, 974, 1765, 2007, 2441, 3105
BseJI GATNNNNATC 3 cut(s) 660, 1425, 3618
BseLI CCNNNNNNNGG 7 cut(s) 49, 827, 883, 1283, 2766, 3381, 3497
BseMI GCAATG 1 cut(s) 1863
BseMII CTCAG 4 cut(s) 614, 1379, 2262, 2916
BseNI ACTGG 8 cut(s) 402, 741, 989, 2128, 2760, 2859, 3144, 3386
BseRI GAGGAG 2 cut(s) 2483, 2966
BseXI GCAGC 6 cut(s) 625, 653, 2370, 2632, 3204, 3392
BsgI GTGCAG 2 cut(s) 2369, 3211
Bsh1236I CGCG 2 cut(s) 605, 1102
Bsh1285I CGRYCG 1 cut(s) 3488
BshFI GGCC 6 cut(s) 97, 114, 697, 3143, 3232, 3313
BshNI GGYRCC 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 448
BsiEI CGRYCG 1 cut(s) 3488
BsiHKAI GWGCWC 4 cut(s) 18, 852, 1119, 1587
BsiHKCI CYCGRG 3 cut(s) 704, 839, 2323
BsiSI CCGG 3 cut(s) 5, 1841, 2459
BslFI GGGAC 1 cut(s) 2297
BslI CCNNNNNNNGG 7 cut(s) 49, 827, 883, 1283, 2766, 3381, 3497
BsmAI GTCTC 5 cut(s) 703, 2339, 2840, 2993, 3448
BsmBI CGTCTC 1 cut(s) 3448
BsmFI GGGAC 1 cut(s) 2297
BsnI GGCC 6 cut(s) 97, 114, 697, 3143, 3232, 3313
Bso31I GGTCTC 1 cut(s) 2339
BsoBI CYCGRG 3 cut(s) 704, 839, 2323
Bsp1286I GDGCHC 4 cut(s) 18, 852, 1119, 1587
Bsp13I TCCGGA 1 cut(s) 2458
Bsp1407I TGTACA 2 cut(s) 1048, 1105
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 9 cut(s) 603, 605, 745, 1085, 1150, 3027, 3310, 3408, 3491
BspANI GGCC 6 cut(s) 97, 114, 697, 3143, 3232, 3313
BspCNI CTCAG 4 cut(s) 615, 1380, 2261, 2917
BspDI ATCGAT 1 cut(s) 448
BspEI TCCGGA 1 cut(s) 2458
BspFNI CGCG 2 cut(s) 605, 1102
BspHI TCATGA 3 cut(s) 1018, 1225, 2089
BspLI GGNNCC 2 cut(s) 158, 669
BspMAI CTGCAG 2 cut(s) 643, 3517
BspPI GGATC 6 cut(s) 114, 203, 760, 1373, 1832, 3614
BspQI GCTCTTC 1 cut(s) 1592
BspT107I GGYRCC 1 cut(s) 156
BspTNI GGTCTC 1 cut(s) 2339
BsrDI GCAATG 1 cut(s) 1863
BsrGI TGTACA 2 cut(s) 1048, 1105
BsrI ACTGG 8 cut(s) 402, 741, 989, 2128, 2760, 2859, 3144, 3386
BssECI CCNNGG 8 cut(s) 502, 603, 692, 820, 821, 1325, 3459, 3482
BssNI GRCGYC 3 cut(s) 157, 2931, 3441
BssSI CACGAG 1 cut(s) 1659
BssT1I CCWWGG 2 cut(s) 502, 3459
Bst2BI CACGAG 1 cut(s) 1659
Bst2UI CCWGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
Bst4CI ACNGT 5 cut(s) 202, 1919, 3045, 3182, 3486
Bst6I CTCTTC 3 cut(s) 142, 1592, 3483
BstACI GRCGYC 3 cut(s) 157, 2931, 3441
BstAPI GCANNNNNTGC 2 cut(s) 656, 1082
BstAUI TGTACA 2 cut(s) 1048, 1105
BstBAI YACGTR 1 cut(s) 2567
BstC8I GCNNGC 2 cut(s) 1908, 2242
BstDSI CCRYGG 4 cut(s) 502, 603, 692, 3482
BstENI CCTNNNNNAGG 2 cut(s) 47, 881
BstF5I GGATG 8 cut(s) 187, 338, 342, 974, 1765, 2007, 2441, 3105
BstFNI CGCG 2 cut(s) 605, 1102
BstH2I RGCGCY 2 cut(s) 160, 1970
BstHHI GCGC 3 cut(s) 159, 1969, 2593
BstMAI GTCTC 5 cut(s) 703, 2339, 2840, 2993, 3448
BstMCI CGRYCG 1 cut(s) 3488
BstMWI GCNNNNNNNGC 4 cut(s) 647, 656, 1082, 3269
BstNI CCWGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
BstNSI RCATGY 5 cut(s) 1000, 1049, 1872, 3574, 3601
BstPAI GACNNNNGTC 1 cut(s) 2309
BstSCI CCNGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
BstSFI CTRYAG 3 cut(s) 175, 639, 3513
BstUI CGCG 2 cut(s) 605, 1102
BstV1I GCAGC 6 cut(s) 625, 653, 2370, 2632, 3204, 3392
BstV2I GAAGAC 2 cut(s) 2438, 2885
BstX2I RGATCY 6 cut(s) 661, 902, 1837, 2425, 2866, 3619
BstYI RGATCY 6 cut(s) 661, 902, 1837, 2425, 2866, 3619
Bsu15I ATCGAT 1 cut(s) 448
BsuI GTATCC 1 cut(s) 2410
BsuRI GGCC 6 cut(s) 97, 114, 697, 3143, 3232, 3313
BsuTUI ATCGAT 1 cut(s) 448
BtgI CCRYGG 4 cut(s) 502, 603, 692, 3482
BtsCI GGATG 8 cut(s) 187, 338, 342, 974, 1765, 2007, 2441, 3105
BtsI GCAGTG 1 cut(s) 3270
BtsIMutI CAGTG 6 cut(s) 395, 2121, 2817, 2852, 3050, 3270
Cac8I GCNNGC 2 cut(s) 1908, 2242
CciI TCATGA 3 cut(s) 1018, 1225, 2089
CfoI GCGC 3 cut(s) 159, 1969, 2593
Cfr13I GGNCC 3 cut(s) 113, 667, 3202
Cfr42I CCGCGG 1 cut(s) 606
ClaI ATCGAT 1 cut(s) 448
CsiI ACCWGGT 1 cut(s) 2062
Csp6I GTAC 8 cut(s) 892, 1049, 1059, 1106, 2367, 3413, 3568, 3595
CviQI GTAC 8 cut(s) 892, 1049, 1059, 1106, 2367, 3413, 3568, 3595
DinI GGCGCC 1 cut(s) 158
DraI TTTAAA 2 cut(s) 1879, 2200
DriI GACNNNNNGTC 2 cut(s) 2352, 3143
EaeI YGGCCR 3 cut(s) 695, 3141, 3311
Eam1104I CTCTTC 3 cut(s) 142, 1592, 3483
Eam1105I GACNNNNNGTC 2 cut(s) 2352, 3143
EarI CTCTTC 3 cut(s) 142, 1592, 3483
Ecl136II GAGCTC 1 cut(s) 1585
Eco130I CCWWGG 2 cut(s) 502, 3459
Eco147I AGGCCT 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 1587
Eco31I GGTCTC 1 cut(s) 2339
Eco32I GATATC 1 cut(s) 2178
Eco47I GGWCC 2 cut(s) 667, 3202
Eco53kI GAGCTC 1 cut(s) 1585
Eco57I CTGAAG 5 cut(s) 470, 630, 2432, 3570, 3600
Eco88I CYCGRG 3 cut(s) 704, 839, 2323
EcoICRI GAGCTC 1 cut(s) 1585
EcoNI CCTNNNNNAGG 2 cut(s) 47, 881
EcoO109I RGGNCCY 1 cut(s) 113
EcoRII CCWGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
EcoRV GATATC 1 cut(s) 2178
EcoT14I CCWWGG 2 cut(s) 502, 3459
EcoT22I ATGCAT 2 cut(s) 337, 2007
EcoT38I GRGCYC 1 cut(s) 1587
EgeI GGCGCC 1 cut(s) 158
EheI GGCGCC 1 cut(s) 158
ErhI CCWWGG 2 cut(s) 502, 3459
Esp3I CGTCTC 1 cut(s) 3448
FalI AAGNNNNNCTT 5 cut(s) 2165, 2197, 2881, 2913, 3609
FaqI GGGAC 1 cut(s) 2297
FauI CCCGC 3 cut(s) 598, 3034, 3401
FauNDI CATATG 2 cut(s) 1279, 2268
FbaI TGATCA 2 cut(s) 85, 2958
FblI GTMKAC 1 cut(s) 733
Fnu4HI GCNGC 8 cut(s) 639, 642, 2384, 2621, 3193, 3311, 3406, 3491
FokI GGATG 8 cut(s) 174, 325, 329, 961, 1772, 2014, 2448, 3092
FriOI GRGCYC 1 cut(s) 1587
Fsp4HI GCNGC 8 cut(s) 639, 642, 2384, 2621, 3193, 3311, 3406, 3491
FspBI CTAG 7 cut(s) 672, 783, 1289, 1832, 1913, 1955, 2442
FspI TGCGCA 1 cut(s) 2592
GlaI GCGC 3 cut(s) 158, 1968, 2592
GluI GCNGC 8 cut(s) 639, 642, 2384, 2621, 3193, 3311, 3406, 3491
GsuI CTGGAG 3 cut(s) 239, 1476, 2709
HaeII RGCGCY 2 cut(s) 160, 1970
HaeIII GGCC 6 cut(s) 97, 114, 697, 3143, 3232, 3313
HapII CCGG 3 cut(s) 5, 1841, 2459
HhaI GCGC 3 cut(s) 159, 1969, 2593
Hin1I GRCGYC 3 cut(s) 157, 2931, 3441
Hin6I GCGC 3 cut(s) 157, 1967, 2591
HinP1I GCGC 3 cut(s) 157, 1967, 2591
HincII GTYRAC 2 cut(s) 734, 2850
HindII GTYRAC 2 cut(s) 734, 2850
HindIII AAGCTT 5 cut(s) 137, 464, 1121, 1575, 2338
HpaII CCGG 3 cut(s) 5, 1841, 2459
HphI GGTGA 5 cut(s) 412, 548, 1855, 2043, 2078
Hpy166II GTNNAC 7 cut(s) 499, 734, 929, 994, 1106, 2765, 2850
Hpy8I GTNNAC 7 cut(s) 499, 734, 929, 994, 1106, 2765, 2850
Hpy99I CGWCG 5 cut(s) 536, 732, 735, 2936, 3530
HpyCH4III ACNGT 5 cut(s) 202, 1919, 3045, 3182, 3486
HpyCH4IV ACGT 7 cut(s) 559, 1057, 1993, 2232, 2566, 2931, 3441
HpyF10VI GCNNNNNNNGC 4 cut(s) 647, 656, 1082, 3269
HpySE526I ACGT 7 cut(s) 559, 1057, 1993, 2232, 2566, 2931, 3441
Hsp92I GRCGYC 3 cut(s) 157, 2931, 3441
HspAI GCGC 3 cut(s) 157, 1967, 2591
KasI GGCGCC 1 cut(s) 156
Kpn2I TCCGGA 1 cut(s) 2458
Ksp22I TGATCA 2 cut(s) 85, 2958
KspI CCGCGG 1 cut(s) 606
LguI GCTCTTC 1 cut(s) 1592
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 6 cut(s) 625, 653, 2370, 2632, 3204, 3392
LweI GCATC 9 cut(s) 208, 322, 340, 344, 646, 1893, 1992, 2426, 2523
MabI ACCWGGT 1 cut(s) 2062
MaeI CTAG 7 cut(s) 672, 783, 1289, 1832, 1913, 1955, 2442
MaeII ACGT 7 cut(s) 559, 1057, 1993, 2232, 2566, 2931, 3441
MaeIII GTNAC 7 cut(s) 196, 355, 1345, 1772, 2049, 2562, 3352
MfeI CAATTG 4 cut(s) 314, 1194, 1218, 1430
MflI RGATCY 6 cut(s) 661, 902, 1837, 2425, 2866, 3619
MhlI GDGCHC 4 cut(s) 18, 852, 1119, 1587
Mly113I GGCGCC 1 cut(s) 157
MlyI GAGTC 4 cut(s) 1106, 1399, 1410, 2057
MmeI TCCRAC 4 cut(s) 1069, 1577, 2287, 2723
Mph1103I ATGCAT 2 cut(s) 337, 2007
MroI TCCGGA 1 cut(s) 2458
MroXI GAANNNNTTC 2 cut(s) 2037, 2892
MslI CAYNNNNRTG 4 cut(s) 507, 1068, 2094, 2150
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 3 cut(s) 5, 1841, 2459
MspR9I CCNGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
MunI CAATTG 4 cut(s) 314, 1194, 1218, 1430
MvaI CCWGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
MvnI CGCG 2 cut(s) 605, 1102
MwoI GCNNNNNNNGC 4 cut(s) 647, 656, 1082, 3269
NarI GGCGCC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 502
NdeI CATATG 2 cut(s) 1279, 2268
NlaIV GGNNCC 2 cut(s) 158, 669
NmuCI GTSAC 1 cut(s) 2049
NsbI TGCGCA 1 cut(s) 2592
NsiI ATGCAT 2 cut(s) 337, 2007
NspI RCATGY 5 cut(s) 1000, 1049, 1872, 3574, 3601
PaeR7I CTCGAG 1 cut(s) 704
PagI TCATGA 3 cut(s) 1018, 1225, 2089
PasI CCCWGGG 1 cut(s) 821
PceI AGGCCT 1 cut(s) 97
PciI ACATGT 2 cut(s) 996, 1045
PciSI GCTCTTC 1 cut(s) 1592
PcsI WCGNNNNNNNCGW 1 cut(s) 3275
PdmI GAANNNNTTC 2 cut(s) 2037, 2892
PflFI GACNNNGTC 2 cut(s) 125, 738
PflMI CCANNNNNTGG 1 cut(s) 3381
PkrI GCNGC 8 cut(s) 640, 643, 2385, 2622, 3194, 3312, 3407, 3492
PleI GAGTC 4 cut(s) 1105, 1398, 1409, 2056
PluTI GGCGCC 1 cut(s) 160
PpsI GAGTC 4 cut(s) 1105, 1398, 1409, 2056
Ppu21I YACGTR 1 cut(s) 2567
PscI ACATGT 2 cut(s) 996, 1045
PshAI GACNNNNGTC 1 cut(s) 2309
PshBI ATTAAT 1 cut(s) 2538
PsiI TTATAA 2 cut(s) 1496, 2228
Psp124BI GAGCTC 1 cut(s) 1587
Psp1406I AACGTT 1 cut(s) 2232
Psp6I CCWGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
PspGI CCWGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
PspN4I GGNNCC 2 cut(s) 158, 669
PspPI GGNCC 3 cut(s) 113, 667, 3202
PstI CTGCAG 2 cut(s) 643, 3517
PsuI RGATCY 6 cut(s) 661, 902, 1837, 2425, 2866, 3619
PsyI GACNNNGTC 2 cut(s) 125, 738
RsaI GTAC 8 cut(s) 893, 1050, 1060, 1107, 2368, 3414, 3569, 3596
RsaNI GTAC 8 cut(s) 892, 1049, 1059, 1106, 2367, 3413, 3568, 3595
RseI CAYNNNNRTG 4 cut(s) 507, 1068, 2094, 2150
SacI GAGCTC 1 cut(s) 1587
SacII CCGCGG 1 cut(s) 606
SalI GTCGAC 1 cut(s) 732
SapI GCTCTTC 1 cut(s) 1592
SatI GCNGC 8 cut(s) 639, 642, 2384, 2621, 3193, 3311, 3406, 3491
Sau96I GGNCC 3 cut(s) 113, 667, 3202
SchI GAGTC 4 cut(s) 1106, 1399, 1410, 2057
ScrFI CCNGG 8 cut(s) 44, 257, 822, 878, 1326, 2055, 2064, 3014
SduI GDGCHC 4 cut(s) 18, 852, 1119, 1587
SexAI ACCWGGT 1 cut(s) 2062
SfaNI GCATC 9 cut(s) 208, 322, 340, 344, 646, 1893, 1992, 2426, 2523
SfcI CTRYAG 3 cut(s) 175, 639, 3513
SfoI GGCGCC 1 cut(s) 158
Sfr274I CTCGAG 1 cut(s) 704
Sfr303I CCGCGG 1 cut(s) 606
SgrBI CCGCGG 1 cut(s) 606
SinI GGWCC 2 cut(s) 667, 3202
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 4 cut(s) 507, 1068, 2094, 2150
SmlI CTYRAG 7 cut(s) 167, 704, 938, 1118, 1689, 2182, 3606
SmoI CTYRAG 7 cut(s) 167, 704, 938, 1118, 1689, 2182, 3606
SpeI ACTAGT 1 cut(s) 2441
SseBI AGGCCT 1 cut(s) 97
SsiI CCGC 9 cut(s) 603, 605, 745, 1085, 1150, 3027, 3310, 3408, 3491
SspDI GGCGCC 1 cut(s) 156
SspI AATATT 4 cut(s) 1306, 2862, 2881, 3095
SspMI CTAG 7 cut(s) 672, 783, 1289, 1832, 1913, 1955, 2442
SstI GAGCTC 1 cut(s) 1587
StuI AGGCCT 1 cut(s) 97
StyD4I CCNGG 8 cut(s) 42, 255, 820, 876, 1324, 2053, 2062, 3012
StyI CCWWGG 2 cut(s) 502, 3459
TaaI ACNGT 5 cut(s) 202, 1919, 3045, 3182, 3486
TaiI ACGT 7 cut(s) 562, 1060, 1996, 2235, 2569, 2934, 3444
TaqI TCGA 9 cut(s) 448, 705, 733, 1860, 2571, 2838, 2934, 3349, 3360
TatI WGTACW 5 cut(s) 891, 1048, 1105, 3567, 3594
TauI GCSGC 2 cut(s) 3313, 3493
TscAI CASTG 6 cut(s) 402, 2128, 2824, 2859, 3050, 3270
TseFI GTSAC 1 cut(s) 2049
TseI GCWGC 6 cut(s) 638, 641, 2383, 2620, 3192, 3405
Tsp45I GTSAC 1 cut(s) 2049
TspGWI ACGGA 4 cut(s) 41, 875, 2129, 3543
TspRI CASTG 6 cut(s) 402, 2128, 2824, 2859, 3050, 3270
Tth111I GACNNNGTC 2 cut(s) 125, 738
Van91I CCANNNNNTGG 1 cut(s) 3381
VpaK11BI GGWCC 2 cut(s) 667, 3202
VspI ATTAAT 1 cut(s) 2538
XagI CCTNNNNNAGG 2 cut(s) 47, 881
XapI RAATTY 7 cut(s) 915, 1010, 1235, 1294, 1851, 2156, 2909
XbaI TCTAGA 1 cut(s) 1831
XceI RCATGY 5 cut(s) 1000, 1049, 1872, 3574, 3601
XhoI CTCGAG 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 733
XmnI GAANNNNTTC 2 cut(s) 2037, 2892
XspI CTAG 7 cut(s) 672, 783, 1289, 1832, 1913, 1955, 2442
ZraI GACGTC 2 cut(s) 2932, 3442
Zsp2I ATGCAT 2 cut(s) 337, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.