FvH4_5g34560

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008785
Physical Location & Seq
Forward (+)
25223290 .. 25231716
8427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34560.t3

Sequence Viewer

Length: 2922 bp
ATGCCTCACAGAACGACTTACTTCTTCCCGCGGCAATTCCCGGATCGCCGGTTCGATTCGTTGTCATCTTCTTCCTCCTCCTCTTCGAAACCGCTGGTGTCGGAAGACGGCAGCAATCATAATCATCAACATCACGAGAAACAACAGACCAGCAGAAATAATAAGAATGAAAACGACGTCGTCAAAAGTCTCATTGATCGCCACCACCAAAAGTCCAACTCTAACCACCACAACAGCAACAGCAGCGCCAAATTCACAGCTGTGTCCGATCAGTATTTCACGGGCGGACAAAAGACCACCACCACAACTAACCCAACAAATACAAAAAGCGGCGGCGGCAGCACGCAATCGGCCAAGAAGCAACAGCTGGCGGCCATTTCCGACTGGTTCGTCGCCGAGAAGAAGCACAGTTACAAGCAGAGATCCAAATCCGGTCACGTGAGGAGCAAGAGGTCGTCGTTCTCCTGCCACGTGACCGATGAGGATCATGAGCCCCTGCTCCCGCCCGAGGCCGTCGTCGATGTGTCCTCCGCGGTCCCGGACACGACGTCGTTCAAGGTCGACCGGAGCGTTGACCGGGACTTTGACCGGCAGGTGTCGCTGCCGAGGCTGTCGAGCGGGAGCAGCTACGCCGGGAGCTTGTTCTCAGGGACGACGACGCTGGACGGGAACTTCTCCGGCGACGTCAAGGACTCGTCGGCGACGACGGGGCCGGCAGAGGAGGAGGTTGAGGAAGAGGTGAGAGTGGAGGATAGCTTGGCGCAGAGGGCTAAAGAGAGTTACTATTTGCAGCTTCTATTGGCAAAGAGGCTTAGTTATCAAGCTAGTCTTGGTAGTGAGACGTTGCTTTTGCACGACACTGGGCTTGAGGTTACTGATGTCGAGACGGTGTCGTATCGCCTCTGGGTCAGTGGCTGCTTGTCATACAATGAGAAAATATCGGATGGTTTCTACAATATTTTGGGGATGAATCCGTATCTATGGGTGCTGTGCAATGATGTGGAGGAAGGTAAACGTCTACCATCATTGACGTCACTTAGAGCAATGAAACCTGTGGAGACATCAATGGAAGTGGTTCTTGTTGACATACACGGGGACTCTCGCCTCAAGGAACTTCAAGATAAGGCACATGAACTGTATCGTGCTTCAGAGAATACCTTAGTGTTGGTGGAGAAACTTGGGAAGCTTGTCGCCATCTACATGGGGGGTACTTATCCTGTGGAGCAAGGAGATCTACACATGCGCTGGAAAGTGGTTAGCAAGAGATTGAGAGAATTCCAAAAGTGTATTGTGCTCCCCATTGGCAACCTATCAATGGGTCTTTGCAGGCATCGTGCTATTCTCTTCAAGAAATTGGCAGACTACATAGGTTTACCTTGCCGGATAGCTCGTGGTTGCAAGTATTGTGTGTCAGATCATCGGTCTTCTTGTCTTGTTAAAATTGAAGACAGGCAGCTCGTGAGGGAGTATGTGGTTGATCTAGTTGGGGAACCTGGAAATGTCCGTGGCCCAGATTCCTCGATCAATGGATCACTGTCTTCAATACCTTCACCATTTCAAATTTCTCATACCAAAGAGTTCCAACGAATTTATAGCGATAGTGGATTATGTTCCCAAATTATTAACTCAAAGCACACTCGTCCACCTCCTGAAGATCCTCTCTATCCAGGCTACGGGGAGGGAAGTCAGGTTCTGAAACCAAGCAGTTTTGTTACAGTTGATCAAACAATATATGGGCTGGAAGCTCCTCTAATGCCTTTGGAGTTGAAAGCGAATGCTGATCATTGCCTTCTCCAGACACCAATGCCACCTTGTGTACGGGGTGATACTTCTGAAGCTCTTGTTGTGGGAGCTGGTATGGCATCATTAGAGGAATCTGCTAGACTTGGTGAAGAGAATGTTGTTATACAGCAAGCTTACCGGAAGGAGATTGTTGTGTCCAGAAGTCAAGTTAAAAGTAGCGGTGACAAGTCCAGTCAATCAGATCTAGAGGAGGTTGATAGTAAGCTTGAGATTCAAAGTCAATTTTCTGCTGTCACTGTTCCTCAGTACTTGAATCTCGAACCATCTCTTGCTATGGACTGGCTTGAGATAGCATGGGATGAGTTGCATATCAAAGAGCGCGTTGGTGCTGGCTCCTTTGGGACTGTGCATCGTGCTGAATGGCATGGATCGGATGTAGCTGTCAAGGTTTTAACTATTCAAGATTTCCGTGATGATCAGATGAGAGAATTTTTACGCGAGGTTGCAATTATGAAACGTGTCCGCCATCCCAATGTAGTTCTGTTCATGGGTGCAGTTACAAAGCGTCCGCATCTCTCAATCGTCACAGAGTATTTACCAAGGGGTAGTCTTTATCGACTCATACATCGGCCAGCTTCTGGGGAACTTCTGGATCATAGGCGGCGTCTGCGCATGGCATTAGATGTGGCCAAGGGGATCAATTACCTCCATTGTCTTAGCCCTCCTATTGTCCATTGGGATCTTAAATCTCCAAATTTGTTGGTTGATAAAAATTGGACAGCTAAGGTATGCGATTTTGGTTTGTCCAGATTCAAAGCGAACACTTTCATATCATCGAAGTCTGTTGCTGGAACACCCGAGTGGATGGCTCCAGAGTTCCTTCGCGGAGAGCCCTCAAATGAGAAGTCTGATGTTTACAGTTTCGGAGTGATCCTTTGGGAACTTGCGACCATGCAACAACCTTGGAGTGGGCTTAGTCCTGCACAGGTAGTTGGTGCTGTTGCTTTCCAGAATAGAAGGCTTACTATCCCAGCCAATACTCCCCCAATGTTGGCTTCTCTTATGGAAGCCTGCTGGGCCGACGACCCTGCTCAGCGCCCGACATTTAAAAGCATAGTGGAGTCACTGAAGAAGTTGCTGAAATCTCCTCTGCAATGGGCACCGGTGAGTGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

974

Amino Acids

108.54

Weight (kDa)

7.03

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 10 - 207 3.1e-69 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 409 - 663 1.8e-65 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 410 - 661 1.5e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 583
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 4 cut(s) 180, 551, 687, 1034
Acc16I TGCGCA 1 cut(s) 2414
Acc36I ACCTGC 1 cut(s) 583
AccB1I GGYRCC 1 cut(s) 2902
AccB7I CCANNNNNTGG 1 cut(s) 2381
AccBSI CCGCTC 1 cut(s) 618
AccI GTMKAC 2 cut(s) 561, 1018
AccII CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
AcoI YGGCCR 4 cut(s) 351, 372, 2372, 2430
AcsI RAATTY 6 cut(s) 251, 1274, 1560, 1587, 2231, 2497
AcuI CTGAAG 4 cut(s) 1131, 1671, 1854, 2891
AcvI CACGTG 2 cut(s) 439, 472
AcyI GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
AdeI CACNNNGTG 1 cut(s) 1814
AfaI GTAC 3 cut(s) 1210, 1818, 2051
AfiI CCNNNNNNNGG 7 cut(s) 508, 1315, 1673, 2381, 2711, 2794, 2912
AflIII ACRYGT 1 cut(s) 2260
AgeI ACCGGT 1 cut(s) 2905
AhdI GACNNNNNGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 1492, 1666
AjuI GAANNNNNNNTTGG 2 cut(s) 2782, 2814
AleI CACNNNNGTG 2 cut(s) 260, 2602
AloI GAACNNNNNNTCC 4 cut(s) 35, 67, 1674, 1706
Alw21I GWGCWC 1 cut(s) 1296
Alw26I GTCTC 4 cut(s) 194, 833, 878, 1052
AlwNI CAGNNNCTG 3 cut(s) 915, 1693, 2381
Ama87I CYCGRG 2 cut(s) 506, 2600
AoxI GGCC 8 cut(s) 351, 372, 510, 710, 1507, 2372, 2430, 2820
ApeKI GCWGC 8 cut(s) 111, 243, 339, 601, 624, 790, 915, 1453
ApoI RAATTY 6 cut(s) 251, 1274, 1560, 1587, 2231, 2497
ArsI GACNNNNNNTTYG 6 cut(s) 566, 598, 832, 864, 2633, 2665
AsiGI ACCGGT 1 cut(s) 2905
Asp700I GAANNNNTTC 2 cut(s) 1074, 2567
AspLEI GCGC 6 cut(s) 248, 763, 1245, 2124, 2415, 2841
AspS9I GGNCC 4 cut(s) 535, 710, 1508, 2820
AsuC2I CCSGG 4 cut(s) 41, 539, 578, 634
AsuHPI GGTGA 6 cut(s) 751, 1542, 1835, 1901, 1976, 2920
AsuII TTCGAA 1 cut(s) 86
AvaI CYCGRG 2 cut(s) 506, 2600
AvaII GGWCC 1 cut(s) 535
BaeGI GKGCMC 1 cut(s) 2905
BalI TGGCCA 1 cut(s) 2432
BanI GGYRCC 1 cut(s) 2902
BanII GRGCYC 2 cut(s) 495, 2637
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 3 cut(s) 134, 1389, 1457
BbrPI CACGTG 2 cut(s) 439, 472
BbsI GAAGAC 4 cut(s) 111, 1416, 1452, 1530
Bbv12I GWGCWC 1 cut(s) 1296
BbvI GCAGC 8 cut(s) 123, 255, 351, 588, 636, 802, 902, 1465
BccI CCATC 6 cut(s) 938, 1030, 1202, 2074, 2277, 2602
BceAI ACGGC 2 cut(s) 124, 497
BcgI CGANNNNNNTGC 2 cut(s) 2813, 2847
BciT130I CCWGG 2 cut(s) 1494, 1668
BclI TGATCA 3 cut(s) 1720, 1780, 2218
BcnI CCSGG 4 cut(s) 41, 539, 578, 634
BcoDI GTCTC 4 cut(s) 194, 833, 878, 1052
BfaI CTAG 4 cut(s) 825, 1481, 1881, 1988
BfoI RGCGCY 2 cut(s) 249, 2842
BfuAI ACCTGC 1 cut(s) 583
BglI GCCNNNNNGGC 1 cut(s) 2819
BglII AGATCT 2 cut(s) 1231, 1984
BlpI GCTNAGC 1 cut(s) 2835
BmcAI AGTACT 1 cut(s) 2051
Bme1390I CCNGG 6 cut(s) 41, 539, 578, 634, 1494, 1668
Bme18I GGWCC 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 547
BmeT110I CYCGRG 2 cut(s) 506, 2600
BmgT120I GGNCC 4 cut(s) 535, 710, 1508, 2820
BmiI GGNNCC 6 cut(s) 537, 711, 1491, 2137, 2613, 2904
BmrFI CCNGG 6 cut(s) 41, 539, 578, 634, 1494, 1668
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 4 cut(s) 1339, 1871, 2161, 2323
BmuI ACTGGG 1 cut(s) 870
BpiI GAAGAC 4 cut(s) 111, 1416, 1452, 1530
BpmI CTGGAG 2 cut(s) 1778, 2598
Bpu10I CCTNAGC 1 cut(s) 2526
Bpu1102I GCTNAGC 1 cut(s) 2835
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 4 cut(s) 887, 1091, 2030, 2108
BpuMI CCSGG 4 cut(s) 41, 539, 578, 634
BsaAI YACGTR 2 cut(s) 439, 472
BsaBI GATNNNNATC 2 cut(s) 427, 483
BsaHI GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
BsaJI CCNNGG 8 cut(s) 29, 507, 531, 605, 1504, 2342, 2433, 2705
BsaWI WCCGGW 4 cut(s) 431, 564, 1920, 2905
Bsc4I CCNNNNNNNGG 7 cut(s) 508, 1315, 1673, 2381, 2711, 2794, 2912
Bse118I RCCGGY 4 cut(s) 48, 588, 712, 2905
Bse1I ACTGG 4 cut(s) 389, 865, 1974, 2087
Bse3DI GCAATG 4 cut(s) 1000, 1050, 1783, 2903
Bse8I GATNNNNATC 2 cut(s) 427, 483
BseBI CCWGG 2 cut(s) 1494, 1668
BseDI CCNNGG 8 cut(s) 29, 507, 531, 605, 1504, 2342, 2433, 2705
BseGI GGATG 6 cut(s) 949, 972, 2107, 2182, 2269, 2613
BseJI GATNNNNATC 2 cut(s) 427, 483
BseLI CCNNNNNNNGG 7 cut(s) 508, 1315, 1673, 2381, 2711, 2794, 2912
BseMI GCAATG 4 cut(s) 1000, 1050, 1783, 2903
BseMII CTCAG 3 cut(s) 660, 2060, 2849
BseNI ACTGG 4 cut(s) 389, 865, 1974, 2087
BseRI GAGGAG 8 cut(s) 67, 70, 457, 734, 737, 1737, 2006, 2880
BseSI GKGCMC 1 cut(s) 2905
BseXI GCAGC 8 cut(s) 123, 255, 351, 588, 636, 802, 902, 1465
BseYI CCCAGC 2 cut(s) 2773, 2817
BsgI GTGCAG 2 cut(s) 2316, 2709
Bsh1236I CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 8 cut(s) 353, 374, 512, 712, 1509, 2374, 2432, 2822
BshNI GGYRCC 1 cut(s) 2902
BshTI ACCGGT 1 cut(s) 2905
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 1296
BsiHKCI CYCGRG 2 cut(s) 506, 2600
BslFI GGGAC 5 cut(s) 521, 593, 664, 1109, 2158
BslI CCNNNNNNNGG 7 cut(s) 508, 1315, 1673, 2381, 2711, 2794, 2912
BsmAI GTCTC 4 cut(s) 194, 833, 878, 1052
BsmBI CGTCTC 2 cut(s) 833, 878
BsmFI GGGAC 5 cut(s) 521, 593, 664, 1109, 2158
BsmI GAATGC 1 cut(s) 1780
BsnI GGCC 8 cut(s) 353, 374, 512, 712, 1509, 2374, 2432, 2822
BsoBI CYCGRG 2 cut(s) 506, 2600
Bsp119I TTCGAA 1 cut(s) 86
Bsp1286I GDGCHC 4 cut(s) 495, 1296, 2637, 2905
Bsp1720I GCTNAGC 1 cut(s) 2835
BspANI GGCC 8 cut(s) 353, 374, 512, 712, 1509, 2374, 2432, 2822
BspCNI CTCAG 3 cut(s) 659, 2059, 2848
BspFNI CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 6 cut(s) 537, 711, 1491, 2137, 2613, 2904
BspMI ACCTGC 1 cut(s) 583
BspT104I TTCGAA 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 2902
BsrBI CCGCTC 1 cut(s) 618
BsrDI GCAATG 4 cut(s) 1000, 1050, 1783, 2903
BsrFI RCCGGY 4 cut(s) 48, 588, 712, 2905
BsrI ACTGG 4 cut(s) 389, 865, 1974, 2087
BssAI RCCGGY 4 cut(s) 48, 588, 712, 2905
BssECI CCNNGG 8 cut(s) 29, 507, 531, 605, 1504, 2342, 2433, 2705
BssNI GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
BssSI CACGAG 3 cut(s) 134, 1389, 1457
BssT1I CCWWGG 3 cut(s) 2342, 2433, 2705
Bst2BI CACGAG 3 cut(s) 134, 1389, 1457
Bst2UI CCWGG 2 cut(s) 1494, 1668
Bst4CI ACNGT 8 cut(s) 410, 889, 1137, 1536, 1717, 2041, 2149, 2663
Bst6I CTCTTC 4 cut(s) 88, 729, 1349, 1887
BstACI GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
BstBAI YACGTR 2 cut(s) 439, 472
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 8 cut(s) 344, 369, 714, 1328, 1914, 2134, 2376, 2815
BstDEI CTNAG 9 cut(s) 646, 812, 1037, 1159, 2046, 2459, 2526, 2717, 2835
BstDSI CCRYGG 3 cut(s) 29, 531, 1504
BstF5I GGATG 6 cut(s) 949, 972, 2107, 2182, 2269, 2613
BstFNI CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
BstH2I RGCGCY 2 cut(s) 249, 2842
BstHHI GCGC 6 cut(s) 248, 763, 1245, 2124, 2415, 2841
BstMAI GTCTC 4 cut(s) 194, 833, 878, 1052
BstMCI CGRYCG 1 cut(s) 565
BstNI CCWGG 2 cut(s) 1494, 1668
BstNSI RCATGY 1 cut(s) 1243
BstSCI CCNGG 6 cut(s) 39, 537, 576, 632, 1492, 1666
BstSLI GKGCMC 1 cut(s) 2905
BstUI CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
BstV1I GCAGC 8 cut(s) 123, 255, 351, 588, 636, 802, 902, 1465
BstV2I GAAGAC 4 cut(s) 111, 1416, 1452, 1530
BstX2I RGATCY 5 cut(s) 422, 1231, 1654, 1984, 2482
BstXI CCANNNNNNTGG 1 cut(s) 1201
BstYI RGATCY 5 cut(s) 422, 1231, 1654, 1984, 2482
BsuRI GGCC 8 cut(s) 353, 374, 512, 712, 1509, 2374, 2432, 2822
BtgI CCRYGG 3 cut(s) 29, 531, 1504
BtsCI GGATG 6 cut(s) 949, 972, 2107, 2182, 2269, 2613
BtsIMutI CAGTG 5 cut(s) 858, 916, 1532, 2037, 2867
BveI ACCTGC 1 cut(s) 583
Cac8I GCNNGC 8 cut(s) 344, 369, 714, 1328, 1914, 2134, 2376, 2815
CaiI CAGNNNCTG 3 cut(s) 915, 1693, 2381
CciI TCATGA 1 cut(s) 487
CfoI GCGC 6 cut(s) 248, 763, 1245, 2124, 2415, 2841
Cfr10I RCCGGY 4 cut(s) 48, 588, 712, 2905
Cfr13I GGNCC 4 cut(s) 535, 710, 1508, 2820
Cfr42I CCGCGG 2 cut(s) 32, 534
CseI GACGC 3 cut(s) 667, 2297, 2396
Csp6I GTAC 3 cut(s) 1209, 1817, 2050
CspAI ACCGGT 1 cut(s) 2905
CspCI CAANNNNNGTGG 2 cut(s) 1053, 1088
CviAII CATG 9 cut(s) 488, 1130, 1201, 1240, 2097, 2168, 2290, 2416, 2695
CviQI GTAC 3 cut(s) 1209, 1817, 2050
DdeI CTNAG 9 cut(s) 646, 812, 1037, 1159, 2046, 2459, 2526, 2717, 2835
DraI TTTAAA 1 cut(s) 2851
DraIII CACNNNGTG 1 cut(s) 1814
DrdI GACNNNNNNGTC 1 cut(s) 389
DriI GACNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 4 cut(s) 351, 372, 2372, 2430
Eam1104I CTCTTC 4 cut(s) 88, 729, 1349, 1887
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 4 cut(s) 88, 729, 1349, 1887
EciI GGCGGA 2 cut(s) 300, 2255
Eco130I CCWWGG 3 cut(s) 2342, 2433, 2705
Eco24I GRGCYC 2 cut(s) 495, 2637
Eco47I GGWCC 1 cut(s) 535
Eco57I CTGAAG 4 cut(s) 1131, 1671, 1854, 2891
Eco72I CACGTG 2 cut(s) 439, 472
Eco88I CYCGRG 2 cut(s) 506, 2600
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 2 cut(s) 1492, 1666
EcoT14I CCWWGG 3 cut(s) 2342, 2433, 2705
EcoT38I GRGCYC 2 cut(s) 495, 2637
ErhI CCWWGG 3 cut(s) 2342, 2433, 2705
Esp3I CGTCTC 2 cut(s) 833, 878
FaeI CATG 9 cut(s) 491, 1133, 1204, 1243, 2100, 2171, 2293, 2419, 2698
FalI AAGNNNNNCTT 4 cut(s) 813, 845, 1062, 1094
FaqI GGGAC 5 cut(s) 521, 593, 664, 1109, 2158
FatI CATG 9 cut(s) 487, 1129, 1200, 1239, 2096, 2167, 2289, 2415, 2694
FauI CCCGC 3 cut(s) 36, 510, 611
FbaI TGATCA 3 cut(s) 1720, 1780, 2218
FblI GTMKAC 2 cut(s) 561, 1018
FokI GGATG 6 cut(s) 956, 979, 2114, 2189, 2256, 2620
FriOI GRGCYC 2 cut(s) 495, 2637
FspBI CTAG 4 cut(s) 825, 1481, 1881, 1988
FspI TGCGCA 1 cut(s) 2414
GlaI GCGC 6 cut(s) 247, 762, 1244, 2123, 2414, 2840
GsaI CCCAGC 2 cut(s) 2777, 2821
GsuI CTGGAG 2 cut(s) 1778, 2598
HaeII RGCGCY 2 cut(s) 249, 2842
HaeIII GGCC 8 cut(s) 353, 374, 512, 712, 1509, 2374, 2432, 2822
HgaI GACGC 3 cut(s) 667, 2297, 2396
HhaI GCGC 6 cut(s) 248, 763, 1245, 2124, 2415, 2841
Hin1I GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
Hin1II CATG 9 cut(s) 491, 1133, 1204, 1243, 2100, 2171, 2293, 2419, 2698
Hin6I GCGC 6 cut(s) 246, 761, 1243, 2122, 2413, 2839
HinP1I GCGC 6 cut(s) 246, 761, 1243, 2122, 2413, 2839
HincII GTYRAC 3 cut(s) 562, 574, 1084
HindII GTYRAC 3 cut(s) 562, 574, 1084
HindIII AAGCTT 3 cut(s) 1184, 1914, 2006
HphI GGTGA 6 cut(s) 751, 1542, 1835, 1901, 1976, 2920
Hpy166II GTNNAC 9 cut(s) 562, 574, 1013, 1019, 1084, 1373, 1643, 1817, 2659
Hpy8I GTNNAC 9 cut(s) 562, 574, 1013, 1019, 1084, 1373, 1643, 1817, 2659
HpyAV CCTTC 6 cut(s) 1001, 1557, 1799, 1918, 2633, 2754
HpyCH4III ACNGT 8 cut(s) 410, 889, 1137, 1536, 1717, 2041, 2149, 2663
HpyCH4IV ACGT 9 cut(s) 177, 438, 471, 548, 684, 842, 1015, 1031, 2260
HpyF3I CTNAG 9 cut(s) 646, 812, 1037, 1159, 2046, 2459, 2526, 2717, 2835
HpySE526I ACGT 9 cut(s) 177, 438, 471, 548, 684, 842, 1015, 1031, 2260
Hsp92I GRCGYC 5 cut(s) 177, 548, 684, 1031, 2407
Hsp92II CATG 9 cut(s) 491, 1133, 1204, 1243, 2100, 2171, 2293, 2419, 2698
HspAI GCGC 6 cut(s) 246, 761, 1243, 2122, 2413, 2839
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
Ksp22I TGATCA 3 cut(s) 1720, 1780, 2218
KspI CCGCGG 2 cut(s) 32, 534
Lsp1109I GCAGC 8 cut(s) 123, 255, 351, 588, 636, 802, 902, 1465
LweI GCATC 4 cut(s) 1339, 1871, 2161, 2323
MaeI CTAG 4 cut(s) 825, 1481, 1881, 1988
MaeII ACGT 9 cut(s) 177, 438, 471, 548, 684, 842, 1015, 1031, 2260
MbiI CCGCTC 1 cut(s) 618
MflI RGATCY 5 cut(s) 422, 1231, 1654, 1984, 2482
MhlI GDGCHC 4 cut(s) 495, 1296, 2637, 2905
MlsI TGGCCA 1 cut(s) 2432
MluNI TGGCCA 1 cut(s) 2432
MlyI GAGTC 4 cut(s) 686, 1091, 2355, 2873
MmeI TCCRAC 4 cut(s) 81, 240, 405, 1606
Mox20I TGGCCA 1 cut(s) 2432
MroNI GCCGGC 1 cut(s) 712
MroXI GAANNNNTTC 2 cut(s) 1074, 2567
MscI TGGCCA 1 cut(s) 2432
MseI TTAA 6 cut(s) 1437, 1623, 1953, 2195, 2487, 2850
MslI CAYNNNNRTG 4 cut(s) 260, 1199, 2274, 2602
Msp20I TGGCCA 1 cut(s) 2432
MspA1I CMGCKG 5 cut(s) 31, 94, 260, 367, 533
MspR9I CCNGG 6 cut(s) 41, 539, 578, 634, 1494, 1668
Mva1269I GAATGC 1 cut(s) 1780
MvaI CCWGG 2 cut(s) 1494, 1668
MvnI CGCG 5 cut(s) 31, 533, 2124, 2241, 2628
NaeI GCCGGC 1 cut(s) 714
NciI CCSGG 4 cut(s) 41, 539, 578, 634
NgoMIV GCCGGC 1 cut(s) 712
NlaIII CATG 9 cut(s) 491, 1133, 1204, 1243, 2100, 2171, 2293, 2419, 2698
NlaIV GGNNCC 6 cut(s) 537, 711, 1491, 2137, 2613, 2904
NmeAIII GCCGAG 2 cut(s) 421, 630
NmuCI GTSAC 7 cut(s) 434, 472, 1032, 1964, 2035, 2326, 2865
NsbI TGCGCA 1 cut(s) 2414
NspI RCATGY 1 cut(s) 1243
NspV TTCGAA 1 cut(s) 86
OliI CACNNNNGTG 2 cut(s) 260, 2602
PagI TCATGA 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 583
PcsI WCGNNNNNNNCGW 2 cut(s) 567, 701
PctI GAATGC 1 cut(s) 1780
PdiI GCCGGC 1 cut(s) 714
PdmI GAANNNNTTC 2 cut(s) 1074, 2567
PfeI GAWTC 7 cut(s) 56, 970, 1514, 1874, 2014, 2056, 2553
PflFI GACNNNGTC 2 cut(s) 179, 889
PflMI CCANNNNNTGG 1 cut(s) 2381
PfoI TCCNGGA 2 cut(s) 39, 537
PinAI ACCGGT 1 cut(s) 2905
PleI GAGTC 4 cut(s) 686, 1091, 2355, 2872
PmaCI CACGTG 2 cut(s) 439, 472
PmlI CACGTG 2 cut(s) 439, 472
PpsI GAGTC 4 cut(s) 686, 1091, 2355, 2872
Ppu21I YACGTR 2 cut(s) 439, 472
Psp6I CCWGG 2 cut(s) 1492, 1666
PspCI CACGTG 2 cut(s) 439, 472
PspFI CCCAGC 2 cut(s) 2773, 2817
PspGI CCWGG 2 cut(s) 1492, 1666
PspN4I GGNNCC 6 cut(s) 537, 711, 1491, 2137, 2613, 2904
PspPI GGNCC 4 cut(s) 535, 710, 1508, 2820
PstNI CAGNNNCTG 3 cut(s) 915, 1693, 2381
PsuI RGATCY 5 cut(s) 422, 1231, 1654, 1984, 2482
PsyI GACNNNGTC 2 cut(s) 179, 889
PvuII CAGCTG 2 cut(s) 260, 367
RsaI GTAC 3 cut(s) 1210, 1818, 2051
RsaNI GTAC 3 cut(s) 1209, 1817, 2050
RseI CAYNNNNRTG 4 cut(s) 260, 1199, 2274, 2602
SacII CCGCGG 2 cut(s) 32, 534
SalI GTCGAC 1 cut(s) 560
SaqAI TTAA 6 cut(s) 1437, 1623, 1953, 2195, 2487, 2850
Sau96I GGNCC 4 cut(s) 535, 710, 1508, 2820
ScaI AGTACT 1 cut(s) 2051
SchI GAGTC 4 cut(s) 686, 1091, 2355, 2873
ScrFI CCNGG 6 cut(s) 41, 539, 578, 634, 1494, 1668
SduI GDGCHC 4 cut(s) 495, 1296, 2637, 2905
SfaNI GCATC 4 cut(s) 1339, 1871, 2161, 2323
Sfr303I CCGCGG 2 cut(s) 32, 534
SfuI TTCGAA 1 cut(s) 86
SgrAI CRCCGGYG 1 cut(s) 2905
SgrBI CCGCGG 2 cut(s) 32, 534
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 4 cut(s) 260, 1199, 2274, 2602
SmlI CTYRAG 4 cut(s) 866, 1106, 2009, 2087
SmoI CTYRAG 4 cut(s) 866, 1106, 2009, 2087
SspI AATATT 1 cut(s) 958
SspMI CTAG 4 cut(s) 825, 1481, 1881, 1988
StyD4I CCNGG 6 cut(s) 39, 537, 576, 632, 1492, 1666
StyI CCWWGG 3 cut(s) 2342, 2433, 2705
TaaI ACNGT 8 cut(s) 410, 889, 1137, 1536, 1717, 2041, 2149, 2663
TaiI ACGT 9 cut(s) 180, 441, 474, 551, 687, 845, 1018, 1034, 2263
TaqII GACCGA 2 cut(s) 491, 1410
TatI WGTACW 1 cut(s) 2049
TauI GCSGC 6 cut(s) 34, 333, 336, 339, 374, 2407
TfiI GAWTC 7 cut(s) 56, 970, 1514, 1874, 2014, 2056, 2553
Tru1I TTAA 6 cut(s) 1437, 1623, 1953, 2195, 2487, 2850
Tru9I TTAA 6 cut(s) 1437, 1623, 1953, 2195, 2487, 2850
TscAI CASTG 5 cut(s) 865, 916, 1539, 2044, 2874
TseFI GTSAC 7 cut(s) 434, 472, 1032, 1964, 2035, 2326, 2865
TseI GCWGC 8 cut(s) 111, 243, 339, 601, 624, 790, 915, 1453
Tsp45I GTSAC 7 cut(s) 434, 472, 1032, 1964, 2035, 2326, 2865
TspDTI ATGAA 7 cut(s) 183, 983, 1061, 1146, 2270, 2278, 2560
TspGWI ACGGA 3 cut(s) 963, 1493, 2201
TspRI CASTG 5 cut(s) 865, 916, 1539, 2044, 2874
Tth111I GACNNNGTC 2 cut(s) 179, 889
Van91I CCANNNNNTGG 1 cut(s) 2381
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 6 cut(s) 251, 1274, 1560, 1587, 2231, 2497
XbaI TCTAGA 1 cut(s) 1987
XceI RCATGY 1 cut(s) 1243
XmiI GTMKAC 2 cut(s) 561, 1018
XmnI GAANNNNTTC 2 cut(s) 1074, 2567
XspI CTAG 4 cut(s) 825, 1481, 1881, 1988
ZraI GACGTC 4 cut(s) 178, 549, 685, 1032
ZrmI AGTACT 1 cut(s) 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.