MD15G1387500.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008785
Physical Location & Seq
Reverse (-)
47908436 .. 47915921
7486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1387500.v1.1.491

Sequence Viewer

Length: 3000 bp
ATGCCTCACAAAACGACTTACTTCTTCCCGAGGCAATTCCCAGATCTAAGGTTTGATTCGTCGTCCAAACAGCTGTTGCAAGAAGATCATCACGATCACGAGAAAGGAAGCAAAGACAGCGACACTGCCACCACCTCCACCACCACCACCACCACAACCGCGACTAGAGATAGAGATGATCACCAACAAAAGGTGCCCAAAACCGCCACCAGCAACTTGTACAAATCCTCAGCCACTACCACTTCTCTGTCTTCCGATTTTCAGTACTTCACGGGCCACGTCCTCGAAAGGAAGCCACCGCAGTTGGCCACCTTTTGCGACTGGTTCCGTCATGACAAGAAGGGGACTAGTACTCGACCCAAGCCATCGAGTACTACTGGTCACGTGAGGGGCCCAAGGAGGTTGTCATCCTCCTCATCATCCTCATGCCACCTTGTTGATGAGGATAGCGAGCTGTTGCTCCCGTGTCTGCCTCGTCATTATCATGTTGATCACTATCACGATCATCATGTGTCCGAGGCTGGCCCACCGCAGATGGCGCCGGAGTCCACCACGATGGCTATGCTCAAGGACCGGAGTGTTGACCGGAGCTTTGACCGGCAGGTGTCGCTGCCGAGGGTTTCGAGCGGGAGTAGCTATGCGGGGAGCTTGTTTTCAGGAACGACGACGTTGGATGGGAACTTCTCTGGGGATGTTAAGGACTCGTCCGTGACAACGAGGCATTTGGAAGTGGAGGAAGAGGAGGAGGTGGGGGAGGAGAGTGCTAAAGTGAGCTTGTCGCAGAGGTCTAAGGAGAGTTATTATTTGCAGCTTCTTTTGGCCAAGAGGCTTAGTTCTCAGGCTACTCTGGGTGCTGAAACATTGCTCTTGCACGAGACTCGTACTGAACTTGAGGTTACGGATGCTGAGACGGTGTCGTACCGGCTCTGGGTCAGTGGCTGCTTGTCTTACAATGACAAGATATCAGATGGTTTCTACAACATTTTGGGGATGAATCCATATCTTTGGGTGCTGTGCAATGATGTGGAAGAGGGTAAACGTATACCGTCCTTGATGTCACTTAAAGAAGTTAAACCTGGTGAGACATCAATGGAGGTACTTCTTGTCGATAAAAATGGTGACTCTCGCCTGAAGGAGCTTCAAGATAAAGCACATCAACTATCGTGCTCTTCAGAGAATACTTTAATATTGGTGGAGAAACTTGGCAAGCTTGTTGCAGTCTATATGGGGGGTAATTATCCCGCGGAGCAAGGAGATCTACACACGCGTTGGCAAGTGGTTAGCAAGAGATTGAGGGATTTTCATAAGTGTATTGTGCTCCCAATTGGCATCCTATCAATGGGGCTTTGCCGGCATCGTGCCATTCTTTTCAAGAAATTGGCAGACCACATAGGTTTGCCTTGTCGCATAGCTCGTGGTTGTAAGTATTGTGTAGCAGATCATCGGTCCTCTTGTCTTGTCAAAATTGAAGATGACAGGAAGCTTTTGAGGGAATATGTGGTTGATCTAGTTGGGGAACCAGGAAACCTTCACGGTCCAGACTCTTCGATAAATGGAGGAACTCTTTCTTCTATACCTTCACCATTTCAAAATTCTCACCTAAAAGAATCCCAACAACCTTACAAGGATAGTGGATTATTTTGCCAATTCACAAACTCAAAGCACTCATATGCTCCTCCCGAAGATCCCTTTTATGCAGGTTCTTCAATCCTTAAAGATCAATCTCTTACAGTCGGTGGGAAGGGAGGTCACGTCATCAAGGAAACCAATTTGCTTCCGGGTGATCAAACTAGACTTGGGTGGGAATCTTCTCTGATGCCTTTGGGGTTGAAGGGAAACACTCCACATTGCCTTGAGGGCGATTCTTCGGAAGCTCTTGATATTACTGCAACCGGTGCTGTCGCATCATTAGAGGAATGTGCTAGACTTCGCGAAGAAAATGCTGTTATACAACAAGCTTACAGAAAAGATATTGTTGTATCAAGAAGTCAGTTTATAAGTAACAGTGTCGACCAATGTAAAGTAACTTTGTACAACCAATCAGATCTAGAGGGGGTTCATAGTGAACTTGTGAAACAAGGTAGAATTTCTGCTGTGACTATTCCAAGGTACGTGAATCTTGAACCATCTCTCGCAATGGACTGGCTTGAGATCGCGTGGGACGAGTTGAATATCAAAGAGCGTGTTGGTGCTGGCTCATTTGGGACGGTGCATCGTGCAGAATGGAATGGATCGGATGTTGCCGTCAAGGTTCTAACTGTTCAGGATTTCCATGATGATCAATTGAAAGAGTTTTTACGTGAGGTTGCAATTATGAAACGTGTTCGTCATCCAAACGTAGTTCTTTTCATGGGTGCGGTTACAAAGCGTCCTCATCTGTCCATAGTGACTGAATATCTGCCAAGGGGTAGTCTTTATCGCCTCATTCACAGGCCAGCTTCCGGGGAACTTCTGGATCAGAGGAGGCGGTTGCGATTGGCATTGGATGTGGCCAAGGGGATCAATTACCTCCATTGTCTTAACCCTCCTATTGTCCATTGGGATCTTAAGTCTCCAAATTTGTTGGTTGATAAAAATTGGACAGCAAAGGTATGCGATTTTGGGTTGTCCAGATTTAAGGCGAACACTTTCATATCATCCAAGTCCGTTGCTGGAACACCTGAGTGGATGGCTCCAGAATTCCTTCGCGGAGAGCCCTCAAATGAGAAGTCCGATGTTTACAGTTTCGGAGTGATCCTATGGGAACTTGTAACCATGGAACAACCTTGGAATGGTCTTAGCCCCGCCCAGGTAGTCGGAGCAGTCGCTTTCCAGAACAGAAGGCTTGCTATCCCTGTTAATACTCCTCCAATGTTGGCTTCGCTTATGGAATCCTGCTGGGCTGATGATCCGGCCCAGCGCCCTTCTTTTGGTAGCATAGTTGAGTCACTAAAGAGGCTGCTGAAGTCTCCTCTGCAATCGGTTCCGGTGGCTGGAACATCGCCTTCTGCCCGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

1000

Amino Acids

111.26

Weight (kDa)

6.61

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 302 - 511 8e-75 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 724 - 977 4e-64 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 724 - 975 4.2e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1997
AarI CACCTGC 1 cut(s) 592
Acc36I ACCTGC 2 cut(s) 592, 1688
AccB1I GGYRCC 2 cut(s) 193, 538
AccBSI CCGCTC 1 cut(s) 627
AccI GTMKAC 2 cut(s) 1042, 2010
AccII CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
AclWI GGATC 7 cut(s) 1679, 2239, 2463, 2507, 2550, 2728, 2882
AcoI YGGCCR 3 cut(s) 306, 819, 2490
AcsI RAATTY 4 cut(s) 1591, 2085, 2557, 2678
AcuI CTGAAG 3 cut(s) 1151, 1155, 2963
AcvI CACGTG 1 cut(s) 385
AcyI GRCGYC 1 cut(s) 539
AfaI GTAC 9 cut(s) 221, 266, 352, 373, 883, 920, 1098, 2033, 2111
AfiI CCNNNNNNNGG 8 cut(s) 190, 928, 1339, 2441, 2771, 2788, 2909, 2972
AflII CTTAAG 1 cut(s) 2546
AflIII ACRYGT 2 cut(s) 1265, 2320
AgeI ACCGGT 1 cut(s) 1892
AgsI TTSAA 9 cut(s) 1142, 1372, 1469, 1589, 1707, 1831, 2123, 2170, 2287
AhlI ACTAGT 1 cut(s) 347
AjiI CACGTC 2 cut(s) 280, 1753
AjnI CCWGG 3 cut(s) 1075, 1519, 2787
AjuI GAANNNNNNNTTGG 2 cut(s) 2842, 2874
AleI CACNNNNGTG 1 cut(s) 2662
Alw21I GWGCWC 2 cut(s) 1169, 1320
Alw26I GTCTC 5 cut(s) 869, 902, 1076, 2556, 2952
AlwI GGATC 7 cut(s) 1679, 2239, 2463, 2507, 2550, 2728, 2882
AlwNI CAGNNNCTG 1 cut(s) 939
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 8 cut(s) 274, 306, 391, 523, 819, 2432, 2490, 2892
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 4 cut(s) 610, 808, 939, 2938
ApoI RAATTY 4 cut(s) 1591, 2085, 2557, 2678
ArsI GACNNNNNNTTYG 4 cut(s) 575, 607, 2693, 2725
AsiGI ACCGGT 1 cut(s) 1892
Asp700I GAANNNNTTC 3 cut(s) 1564, 2627, 2682
AspLEI GCGC 2 cut(s) 541, 2901
AspS9I GGNCC 9 cut(s) 274, 391, 392, 524, 571, 1446, 1535, 2893, 2994
AsuC2I CCSGG 3 cut(s) 1779, 2443, 2992
AsuHPI GGTGA 6 cut(s) 173, 1091, 1130, 1572, 1589, 1793
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 4 cut(s) 571, 1446, 1535, 2994
BaeGI GKGCMC 2 cut(s) 198, 395
BalI TGGCCA 3 cut(s) 308, 821, 2492
BanI GGYRCC 2 cut(s) 193, 538
BanII GRGCYC 2 cut(s) 395, 2697
BauI CACGAG 3 cut(s) 98, 872, 1413
BbrPI CACGTG 1 cut(s) 385
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 1169, 1320
BbvCI CCTCAGC 1 cut(s) 229
BbvI GCAGC 4 cut(s) 597, 820, 926, 2925
BccI CCATC 7 cut(s) 373, 529, 550, 668, 962, 2134, 2662
BceAI ACGGC 1 cut(s) 2228
BcgI CGANNNNNNTGC 6 cut(s) 544, 578, 860, 894, 1922, 1956
BciT130I CCWGG 3 cut(s) 1077, 1521, 2789
BclI TGATCA 4 cut(s) 178, 490, 1783, 2278
BcnI CCSGG 3 cut(s) 1779, 2443, 2992
BcoDI GTCTC 5 cut(s) 869, 902, 1076, 2556, 2952
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 6 cut(s) 165, 348, 1508, 1791, 1923, 2048
BfoI RGCGCY 2 cut(s) 542, 2902
BfrI CTTAAG 1 cut(s) 2546
BfuAI ACCTGC 2 cut(s) 592, 1688
BglI GCCNNNNNGGC 1 cut(s) 1857
BglII AGATCT 3 cut(s) 43, 1255, 2044
BisI GCNGC 4 cut(s) 611, 809, 940, 2939
BlsI GCNGC 4 cut(s) 612, 810, 941, 2940
BmcAI AGTACT 3 cut(s) 266, 352, 373
Bme1390I CCNGG 6 cut(s) 1077, 1521, 1779, 2443, 2789, 2992
Bme18I GGWCC 4 cut(s) 571, 1446, 1535, 2994
BmeT110I CYCGRG 1 cut(s) 28
BmgBI CACGTC 2 cut(s) 280, 1753
BmgT120I GGNCC 9 cut(s) 274, 391, 392, 524, 571, 1446, 1535, 2893, 2994
BmiI GGNNCC 8 cut(s) 195, 326, 392, 393, 540, 1518, 2673, 2964
BmrFI CCNGG 6 cut(s) 1077, 1521, 1779, 2443, 2789, 2992
BmsI GCATC 6 cut(s) 892, 1338, 1363, 1806, 1913, 2221
BpiI GAAGAC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 2658
Bpu10I CCTNAGC 1 cut(s) 229
BpuEI CTTGAG 4 cut(s) 551, 911, 1874, 2168
BpuMI CCSGG 3 cut(s) 1779, 2443, 2992
BsaAI YACGTR 3 cut(s) 385, 2113, 2300
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 539
BsaWI WCCGGW 4 cut(s) 573, 585, 1892, 2965
BsaXI ACNNNNNCTCC 2 cut(s) 1737, 1767
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 928, 1339, 2441, 2771, 2788, 2909, 2972
Bse118I RCCGGY 4 cut(s) 597, 921, 1350, 1892
Bse1I ACTGG 3 cut(s) 326, 382, 2147
Bse3DI GCAATG 4 cut(s) 860, 1024, 1846, 2142
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 3 cut(s) 1077, 1521, 2789
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 8 cut(s) 190, 928, 1339, 2441, 2771, 2788, 2909, 2972
BseMI GCAATG 4 cut(s) 860, 1024, 1846, 2142
BseMII CTCAG 4 cut(s) 243, 851, 897, 2652
BseNI ACTGG 3 cut(s) 326, 382, 2147
BseRI GAGGAG 8 cut(s) 403, 755, 758, 770, 1665, 2476, 2835, 2940
BseSI GKGCMC 2 cut(s) 198, 395
BseXI GCAGC 4 cut(s) 597, 820, 926, 2925
BseYI CCCAGC 2 cut(s) 2877, 2895
BsgI GTGCAG 1 cut(s) 2238
Bsh1236I CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
BshFI GGCC 8 cut(s) 276, 308, 393, 525, 821, 2434, 2492, 2894
BshNI GGYRCC 2 cut(s) 193, 538
BshTI ACCGGT 1 cut(s) 1892
BsiHKAI GWGCWC 2 cut(s) 1169, 1320
BsiHKCI CYCGRG 1 cut(s) 28
BslFI GGGAC 3 cut(s) 358, 2174, 2218
BslI CCNNNNNNNGG 8 cut(s) 190, 928, 1339, 2441, 2771, 2788, 2909, 2972
BsmAI GTCTC 5 cut(s) 869, 902, 1076, 2556, 2952
BsmBI CGTCTC 1 cut(s) 902
BsmFI GGGAC 3 cut(s) 358, 2174, 2218
BsnI GGCC 8 cut(s) 276, 308, 393, 525, 821, 2434, 2492, 2894
BsoBI CYCGRG 1 cut(s) 28
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 5 cut(s) 198, 395, 1169, 1320, 2697
Bsp1407I TGTACA 2 cut(s) 219, 2031
Bsp19I CCATGG 1 cut(s) 2754
Bsp68I TCGCGA 1 cut(s) 1932
BspANI GGCC 8 cut(s) 276, 308, 393, 525, 821, 2434, 2492, 2894
BspCNI CTCAG 4 cut(s) 242, 850, 898, 2653
BspFNI CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 8 cut(s) 195, 326, 392, 393, 540, 1518, 2673, 2964
BspMI ACCTGC 2 cut(s) 592, 1688
BspPI GGATC 7 cut(s) 1679, 2239, 2463, 2507, 2550, 2728, 2882
BspQI GCTCTTC 1 cut(s) 1174
BspT107I GGYRCC 2 cut(s) 193, 538
BspTI CTTAAG 1 cut(s) 2546
BsrBI CCGCTC 1 cut(s) 627
BsrDI GCAATG 4 cut(s) 860, 1024, 1846, 2142
BsrFI RCCGGY 4 cut(s) 597, 921, 1350, 1892
BsrGI TGTACA 2 cut(s) 219, 2031
BsrI ACTGG 3 cut(s) 326, 382, 2147
BssAI RCCGGY 4 cut(s) 597, 921, 1350, 1892
BssNAI GTATAC 1 cut(s) 1043
BssNI GRCGYC 1 cut(s) 539
BssSI CACGAG 3 cut(s) 98, 872, 1413
BssT1I CCWWGG 6 cut(s) 395, 2105, 2402, 2493, 2754, 2765
Bst1107I GTATAC 1 cut(s) 1043
Bst2BI CACGAG 3 cut(s) 98, 872, 1413
Bst2UI CCWGG 3 cut(s) 1077, 1521, 2789
Bst4CI ACNGT 8 cut(s) 913, 1047, 1535, 1732, 2006, 2209, 2260, 2723
Bst6I CTCTTC 4 cut(s) 732, 1023, 1174, 1549
BstACI GRCGYC 1 cut(s) 539
BstAFI CTTAAG 1 cut(s) 2546
BstAPI GCANNNNNTGC 1 cut(s) 1895
BstAUI TGTACA 2 cut(s) 219, 2031
BstBAI YACGTR 3 cut(s) 385, 2113, 2300
BstC8I GCNNGC 7 cut(s) 452, 523, 1208, 1352, 2194, 2436, 2826
BstDEI CTNAG 8 cut(s) 47, 229, 789, 830, 837, 906, 2661, 2777
BstDSI CCRYGG 2 cut(s) 1242, 2754
BstFNI CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
BstH2I RGCGCY 2 cut(s) 542, 2902
BstHHI GCGC 2 cut(s) 541, 2901
BstMAI GTCTC 5 cut(s) 869, 902, 1076, 2556, 2952
BstMWI GCNNNNNNNGC 7 cut(s) 117, 538, 607, 633, 1351, 1857, 1895
BstNI CCWGG 3 cut(s) 1077, 1521, 2789
BstSCI CCNGG 6 cut(s) 1075, 1519, 1777, 2441, 2787, 2990
BstSLI GKGCMC 2 cut(s) 198, 395
BstUI CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
BstV1I GCAGC 4 cut(s) 597, 820, 926, 2925
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 5 cut(s) 43, 1255, 1684, 2044, 2542
BstXI CCANNNNNNTGG 2 cut(s) 556, 1005
BstYI RGATCY 5 cut(s) 43, 1255, 1684, 2044, 2542
BstZ17I GTATAC 1 cut(s) 1043
BsuRI GGCC 8 cut(s) 276, 308, 393, 525, 821, 2434, 2492, 2894
BtgI CCRYGG 2 cut(s) 1242, 2754
BtgZI GCGATG 1 cut(s) 2964
BtrI CACGTC 2 cut(s) 280, 1753
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 3 cut(s) 123, 940, 2011
BtuMI TCGCGA 1 cut(s) 1932
BveI ACCTGC 2 cut(s) 592, 1688
Cac8I GCNNGC 7 cut(s) 452, 523, 1208, 1352, 2194, 2436, 2826
CaiI CAGNNNCTG 1 cut(s) 939
CciI TCATGA 1 cut(s) 331
CfoI GCGC 2 cut(s) 541, 2901
Cfr10I RCCGGY 4 cut(s) 597, 921, 1350, 1892
Cfr13I GGNCC 9 cut(s) 274, 391, 392, 524, 571, 1446, 1535, 2893, 2994
Cfr42I CCGCGG 1 cut(s) 1245
CseI GACGC 1 cut(s) 2357
CsiI ACCWGGT 1 cut(s) 1075
Csp6I GTAC 9 cut(s) 220, 265, 351, 372, 882, 919, 1097, 2032, 2110
CspAI ACCGGT 1 cut(s) 1892
CspCI CAANNNNNGTGG 4 cut(s) 285, 320, 1612, 1647
CviAII CATG 7 cut(s) 332, 426, 485, 509, 2273, 2350, 2755
CviQI GTAC 9 cut(s) 220, 265, 351, 372, 882, 919, 1097, 2032, 2110
DdeI CTNAG 8 cut(s) 47, 229, 789, 830, 837, 906, 2661, 2777
DinI GGCGCC 1 cut(s) 540
EaeI YGGCCR 3 cut(s) 306, 819, 2490
Eam1104I CTCTTC 4 cut(s) 732, 1023, 1174, 1549
EarI CTCTTC 4 cut(s) 732, 1023, 1174, 1549
Eco130I CCWWGG 6 cut(s) 395, 2105, 2402, 2493, 2754, 2765
Eco24I GRGCYC 2 cut(s) 395, 2697
Eco32I GATATC 1 cut(s) 963
Eco47I GGWCC 4 cut(s) 571, 1446, 1535, 2994
Eco57I CTGAAG 3 cut(s) 1151, 1155, 2963
Eco72I CACGTG 1 cut(s) 385
Eco88I CYCGRG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 391
EcoRI GAATTC 1 cut(s) 2678
EcoRII CCWGG 3 cut(s) 1075, 1519, 2787
EcoRV GATATC 1 cut(s) 963
EcoT14I CCWWGG 6 cut(s) 395, 2105, 2402, 2493, 2754, 2765
EcoT38I GRGCYC 2 cut(s) 395, 2697
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
ErhI CCWWGG 6 cut(s) 395, 2105, 2402, 2493, 2754, 2765
Esp3I CGTCTC 1 cut(s) 902
FaeI CATG 7 cut(s) 335, 429, 488, 512, 2276, 2353, 2758
FaqI GGGAC 3 cut(s) 358, 2174, 2218
FatI CATG 7 cut(s) 331, 425, 484, 508, 2272, 2349, 2754
FauI CCCGC 4 cut(s) 620, 634, 1249, 2791
FauNDI CATATG 1 cut(s) 1669
FbaI TGATCA 4 cut(s) 178, 490, 1783, 2278
FblI GTMKAC 2 cut(s) 1042, 2010
Fnu4HI GCNGC 4 cut(s) 611, 809, 940, 2939
FriOI GRGCYC 2 cut(s) 395, 2697
Fsp4HI GCNGC 4 cut(s) 611, 809, 940, 2939
FspBI CTAG 6 cut(s) 165, 348, 1508, 1791, 1923, 2048
GlaI GCGC 2 cut(s) 540, 2900
GluI GCNGC 4 cut(s) 611, 809, 940, 2939
GsaI CCCAGC 2 cut(s) 2881, 2899
GsuI CTGGAG 1 cut(s) 2658
HaeII RGCGCY 2 cut(s) 542, 2902
HaeIII GGCC 8 cut(s) 276, 308, 393, 525, 821, 2434, 2492, 2894
HgaI GACGC 1 cut(s) 2357
HhaI GCGC 2 cut(s) 541, 2901
Hin1I GRCGYC 1 cut(s) 539
Hin1II CATG 7 cut(s) 335, 429, 488, 512, 2276, 2353, 2758
Hin6I GCGC 2 cut(s) 539, 2899
HinP1I GCGC 2 cut(s) 539, 2899
HincII GTYRAC 2 cut(s) 583, 2011
HindII GTYRAC 2 cut(s) 583, 2011
HindIII AAGCTT 3 cut(s) 1208, 1481, 1956
HphI GGTGA 6 cut(s) 173, 1091, 1130, 1572, 1589, 1793
Hpy166II GTNNAC 7 cut(s) 549, 583, 1037, 1043, 2011, 2066, 2719
Hpy8I GTNNAC 7 cut(s) 549, 583, 1037, 1043, 2011, 2066, 2719
Hpy99I CGWCG 3 cut(s) 64, 667, 670
HpyCH4III ACNGT 8 cut(s) 913, 1047, 1535, 1732, 2006, 2209, 2260, 2723
HpyCH4IV ACGT 9 cut(s) 279, 384, 668, 1039, 1752, 2112, 2299, 2320, 2337
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 538, 607, 633, 1351, 1857, 1895
HpyF3I CTNAG 8 cut(s) 47, 229, 789, 830, 837, 906, 2661, 2777
HpySE526I ACGT 9 cut(s) 279, 384, 668, 1039, 1752, 2112, 2299, 2320, 2337
Hsp92I GRCGYC 1 cut(s) 539
Hsp92II CATG 7 cut(s) 335, 429, 488, 512, 2276, 2353, 2758
HspAI GCGC 2 cut(s) 539, 2899
KasI GGCGCC 1 cut(s) 538
KroI GCCGGC 1 cut(s) 1350
KroNI GCCGGC 1 cut(s) 1352
Ksp22I TGATCA 4 cut(s) 178, 490, 1783, 2278
KspI CCGCGG 1 cut(s) 1245
LguI GCTCTTC 1 cut(s) 1174
LmnI GCTCC 9 cut(s) 465, 588, 645, 1135, 1246, 1323, 1678, 2677, 2798
Lsp1109I GCAGC 4 cut(s) 597, 820, 926, 2925
LweI GCATC 6 cut(s) 892, 1338, 1363, 1806, 1913, 2221
MabI ACCWGGT 1 cut(s) 1075
MaeI CTAG 6 cut(s) 165, 348, 1508, 1791, 1923, 2048
MaeII ACGT 9 cut(s) 279, 384, 668, 1039, 1752, 2112, 2299, 2320, 2337
MbiI CCGCTC 1 cut(s) 627
MfeI CAATTG 2 cut(s) 1323, 2282
MflI RGATCY 5 cut(s) 43, 1255, 1684, 2044, 2542
MhlI GDGCHC 5 cut(s) 198, 395, 1169, 1320, 2697
MlsI TGGCCA 3 cut(s) 308, 821, 2492
MluI ACGCGT 1 cut(s) 1265
MluNI TGGCCA 3 cut(s) 308, 821, 2492
Mly113I GGCGCC 1 cut(s) 539
MlyI GAGTC 6 cut(s) 554, 695, 871, 1115, 1535, 2933
MmeI TCCRAC 2 cut(s) 651, 2776
Mox20I TGGCCA 3 cut(s) 308, 821, 2492
MroNI GCCGGC 1 cut(s) 1350
MroXI GAANNNNTTC 3 cut(s) 1564, 2627, 2682
MscI TGGCCA 3 cut(s) 308, 821, 2492
MseI TTAA 9 cut(s) 696, 1062, 1071, 1184, 1713, 2520, 2547, 2616, 2838
MslI CAYNNNNRTG 5 cut(s) 424, 483, 554, 1668, 2662
Msp20I TGGCCA 3 cut(s) 308, 821, 2492
MspA1I CMGCKG 2 cut(s) 73, 1244
MspCI CTTAAG 1 cut(s) 2546
MspR9I CCNGG 6 cut(s) 1077, 1521, 1779, 2443, 2789, 2992
MunI CAATTG 2 cut(s) 1323, 2282
MvaI CCWGG 3 cut(s) 1077, 1521, 2789
MvnI CGCG 6 cut(s) 161, 1244, 1267, 1932, 2156, 2688
MwoI GCNNNNNNNGC 7 cut(s) 117, 538, 607, 633, 1351, 1857, 1895
NaeI GCCGGC 1 cut(s) 1352
NarI GGCGCC 1 cut(s) 539
NciI CCSGG 3 cut(s) 1779, 2443, 2992
NcoI CCATGG 1 cut(s) 2754
NdeI CATATG 1 cut(s) 1669
NgoMIV GCCGGC 1 cut(s) 1350
NlaIII CATG 7 cut(s) 335, 429, 488, 512, 2276, 2353, 2758
NlaIV GGNNCC 8 cut(s) 195, 326, 392, 393, 540, 1518, 2673, 2964
NmeAIII GCCGAG 1 cut(s) 639
NmuCI GTSAC 8 cut(s) 380, 709, 1056, 1118, 1748, 2095, 2386, 2925
NruI TCGCGA 1 cut(s) 1932
OliI CACNNNNGTG 1 cut(s) 2662
PagI TCATGA 1 cut(s) 331
PaqCI CACCTGC 1 cut(s) 592
PciSI GCTCTTC 1 cut(s) 1174
PcsI WCGNNNNNNNCGW 2 cut(s) 1411, 2160
PdiI GCCGGC 1 cut(s) 1352
PdmI GAANNNNTTC 3 cut(s) 1564, 2627, 2682
PfeI GAWTC 7 cut(s) 56, 994, 1607, 1805, 1862, 2116, 2870
PflFI GACNNNGTC 1 cut(s) 913
PinAI ACCGGT 1 cut(s) 1892
PkrI GCNGC 4 cut(s) 612, 810, 941, 2940
PleI GAGTC 6 cut(s) 553, 695, 871, 1115, 1535, 2932
PluTI GGCGCC 1 cut(s) 542
PmaCI CACGTG 1 cut(s) 385
PmlI CACGTG 1 cut(s) 385
PpsI GAGTC 6 cut(s) 553, 695, 871, 1115, 1535, 2932
Ppu21I YACGTR 3 cut(s) 385, 2113, 2300
PsiI TTATAA 1 cut(s) 1997
Psp6I CCWGG 3 cut(s) 1075, 1519, 2787
PspCI CACGTG 1 cut(s) 385
PspFI CCCAGC 2 cut(s) 2877, 2895
PspGI CCWGG 3 cut(s) 1075, 1519, 2787
PspN4I GGNNCC 8 cut(s) 195, 326, 392, 393, 540, 1518, 2673, 2964
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 9 cut(s) 274, 391, 392, 524, 571, 1446, 1535, 2893, 2994
PstNI CAGNNNCTG 1 cut(s) 939
PsuI RGATCY 5 cut(s) 43, 1255, 1684, 2044, 2542
PsyI GACNNNGTC 1 cut(s) 913
PvuII CAGCTG 1 cut(s) 73
RruI TCGCGA 1 cut(s) 1932
RsaI GTAC 9 cut(s) 221, 266, 352, 373, 883, 920, 1098, 2033, 2111
RsaNI GTAC 9 cut(s) 220, 265, 351, 372, 882, 919, 1097, 2032, 2110
RseI CAYNNNNRTG 5 cut(s) 424, 483, 554, 1668, 2662
SacII CCGCGG 1 cut(s) 1245
SalI GTCGAC 1 cut(s) 2009
SapI GCTCTTC 1 cut(s) 1174
SaqAI TTAA 9 cut(s) 696, 1062, 1071, 1184, 1713, 2520, 2547, 2616, 2838
SatI GCNGC 4 cut(s) 611, 809, 940, 2939
Sau96I GGNCC 9 cut(s) 274, 391, 392, 524, 571, 1446, 1535, 2893, 2994
ScaI AGTACT 3 cut(s) 266, 352, 373
SchI GAGTC 6 cut(s) 554, 695, 871, 1115, 1535, 2933
ScrFI CCNGG 6 cut(s) 1077, 1521, 1779, 2443, 2789, 2992
SduI GDGCHC 5 cut(s) 198, 395, 1169, 1320, 2697
SexAI ACCWGGT 1 cut(s) 1075
SfaNI GCATC 6 cut(s) 892, 1338, 1363, 1806, 1913, 2221
SfoI GGCGCC 1 cut(s) 540
Sfr303I CCGCGG 1 cut(s) 1245
SgrBI CCGCGG 1 cut(s) 1245
SinI GGWCC 4 cut(s) 571, 1446, 1535, 2994
SmiMI CAYNNNNRTG 5 cut(s) 424, 483, 554, 1668, 2662
SmlI CTYRAG 5 cut(s) 566, 890, 1853, 2147, 2546
SmoI CTYRAG 5 cut(s) 566, 890, 1853, 2147, 2546
SpeI ACTAGT 1 cut(s) 347
SspDI GGCGCC 1 cut(s) 538
SspI AATATT 1 cut(s) 1188
SspMI CTAG 6 cut(s) 165, 348, 1508, 1791, 1923, 2048
StyD4I CCNGG 6 cut(s) 1075, 1519, 1777, 2441, 2787, 2990
StyI CCWWGG 6 cut(s) 395, 2105, 2402, 2493, 2754, 2765
TaaI ACNGT 8 cut(s) 913, 1047, 1535, 1732, 2006, 2209, 2260, 2723
TaiI ACGT 9 cut(s) 282, 387, 671, 1042, 1755, 2115, 2302, 2323, 2340
TaqI TCGA 7 cut(s) 285, 355, 368, 623, 1107, 1547, 2010
TaqII GACCGA 1 cut(s) 1434
TatI WGTACW 5 cut(s) 219, 264, 350, 371, 2031
TfiI GAWTC 7 cut(s) 56, 994, 1607, 1805, 1862, 2116, 2870
Tru1I TTAA 9 cut(s) 696, 1062, 1071, 1184, 1713, 2520, 2547, 2616, 2838
Tru9I TTAA 9 cut(s) 696, 1062, 1071, 1184, 1713, 2520, 2547, 2616, 2838
TscAI CASTG 3 cut(s) 130, 940, 2011
TseFI GTSAC 8 cut(s) 380, 709, 1056, 1118, 1748, 2095, 2386, 2925
TseI GCWGC 4 cut(s) 610, 808, 939, 2938
Tsp45I GTSAC 8 cut(s) 380, 709, 1056, 1118, 1748, 2095, 2386, 2925
TspDTI ATGAA 6 cut(s) 1007, 1292, 2048, 2330, 2338, 2620
TspGWI ACGGA 4 cut(s) 317, 697, 914, 2635
TspRI CASTG 3 cut(s) 130, 940, 2011
Tth111I GACNNNGTC 1 cut(s) 913
Vha464I CTTAAG 1 cut(s) 2546
VpaK11BI GGWCC 4 cut(s) 571, 1446, 1535, 2994
XapI RAATTY 4 cut(s) 1591, 2085, 2557, 2678
XbaI TCTAGA 1 cut(s) 2047
XmiI GTMKAC 2 cut(s) 1042, 2010
XmnI GAANNNNTTC 3 cut(s) 1564, 2627, 2682
XspI CTAG 6 cut(s) 165, 348, 1508, 1791, 1923, 2048
ZrmI AGTACT 3 cut(s) 266, 352, 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.