MD08G1200300.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008785
Physical Location & Seq
Reverse (-)
25925652 .. 25934006
8355 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1200300.v1.1.491

Sequence Viewer

Length: 2976 bp
ATGCCTCACAGAACGACTTACTTCTTCCCGAGGCAATTCCCGGATCGAAGGTTCGATTCGTCGTCCAAACAGCTGTTGCAAGAAGATCATCACGATCACGAGAAAGTAACCAAAGACAGCGACACTGCCACTACCACCACCACCACAACTGCGGCCAGAGATAGAGATGATTACCAACAAAAGGTGCCCAAAACCGCCACCAGCAACTTGGACAAATCCTCAGCCACTACCACTTCTTCTCTGTCTTCCGATGTTCAGTACTTCACGGGCCACATCGATGAAAGGAAGCCACCGCAGTTGGCCGACTTTCTCAACTGGTTCCTTCATGACAAGAAGGGGACCAGTACTCGATCCAAACCATCGAGTAGTGGCCACCGTCCTCGTCGCGTTAGGGTAACAGGGAGGTTGTCCTCTTCGTGCCACCTTGTTGACGAGGATCGCGAGCTGTTGCTGCCTCGTCGTCATCATCATCATGATGATGATCACGATAATCATGTGGCTGAGGCTGGCCCACCGCAGATGGCGCCGGAGTCCACCACGACTGCTATGCTCAAAGACCGGAGTGTTGACCAGAGCTTTGACCGGCAGGTGTCCCTGCCGAGGGTTTCGAGCGGGAGTAGCTACGCAGGGAGCTTGTTCTCAGGAACGACGACGTTGGATGGGAACTTCTCTGGGGATGTTAAGGACTCGTCAGCGACGACGAGGCATTTGGAAGTGGAGGAAGAGGAGGTGGAACAGGAGAGTGCTAAAGTGAGCTTGTCTCAGAGGTCTAAGCAAAGTTATCATTTGCAGCTTCTTTTGGCCAAGAGGCTTTGTTCTCAAGCTACTCTTGGTGCTGAAACAATGCTTTTGCACGAGACTACTACTCAACTTGAGGTTACTGATGCTGAGACGGTGTCGTATCGGCTCTGGGTCAGTGGCTGCTTGTCTTACAATGACAAGATATCAGATGGTTTCTACAACATTTTAGGGATGAATCCATATCTTTGGGTGATGTGCAATGATGTAGAGGAAGGTAAAAAAATACCGTCCTTGATGTCACTTAAAGAAGTTAAACCTGGTGAGACATCAATGGAGGTGCTTCTTGTCGATAAAAATGATGACGTTCGCCTCAAGGAGCTTCAAGATAAAGCACAGCAACTATCTTGCACTACAGAGAGTACTTTAGTGTTGGTGGAGAAACTTGGCAAGCTTGTCGCAATCTATATGGGGGGTAATTATCCCGCGGAGCAAGGAGATCTACACATGCGTTGGAAGGTGGTTAGCAAGCGATTGAGGGATTTTCACAAGTGTATTGTGCTCCCCATAGGCAGCCTATCAATGGGGCTTTGCAGGCATCGTGCCATTCTTTTCAAGAAATTGGCAGACCACATAGGTTTGCCTTGTCGCATAGCTCGTGGTTGCAAGTATTGTGTGGCAGATCACCGGTCCTCTTGTCTTGTTAAAATTGAAGATAACAGGAAGGTTTTGAGGGAATATGTGGTTGACCTAGTTGGGGAACCAGGAAACCTTCATGGTCCAGACTCCTCGATTAACGGAGGAACTCTTTCTTCTATACCTTCACCACTTCAAATTTCTCATCTAAAAGAATCCAAACAACCTTACAGGGATAGTGAATTATATTGCCAAATCACAAACTCAAAGCACACGGGTGCTCCTCCTGAAGATCCCTTTTATGCAGGCGGTGGGGAGGGAGGTCAAATCATCAAGGAAACCAGTTTGCTTCCAGGTGATCAAACTAGATTTGGGTTGGAATCTTCTCTGATGCGATTGGAATTGACAGGAAACGCTCCGCATTGCCTTGTTCAGAGTGACATCCCACCTTGTGTCCAGGGTGATGCTTCGGAAGCTCTTGATGTTACTGCTACTACTGCTGTGGCATCATTAGAGGAATGTGCTAGACTTAGCGAAGAAAATGTTGTTGTACAACAAGCTTACAGAAAGGAGATTGTTGTATCAAGAAATCAGGTTATAAGCAACAGTGTTGAGCAACCTAAAGTAACTTTGTACAGCCAATCAGATCTAGAGGGCGTTCATAGTGAACTTGTGAAACAAGGTAGAATTACTGCTGTGACTACCCCAAGGTACTTGAATCTTGAACCGTCTCTCGCAATGGACTGGCTTGAGATCTCATGGGACGAGTTGAATATCAAAGAGCGTGTTGGTGCTGGCTCATTTGGGACAGTGCATCGTGCTGAATGGAATGGATCGGATGTTGCTGTTAAGGTTCTAACTGTTCAGGATTTCCATGATGATCAATTGAAAGACTTTTTACGAGAGGTTGCAATTATGAAACGTGTTCGTCATCCAAACGTAGTTCTTTTCATGGGTGCGGTTACAAAGCGTCCTCATCTATCTATAGTGACTGAATATCTGCCAAGGGGTAGTCTTTATCGCCTCATTCACAGGCCAGCTTCCGGGGAACTTATGGATCAGAGGAGGCGGTTGCGAATGGCACTAGATGTGGCCAAGGGTATCAATTACCTCCATTGTCTTAACCCTCCTATTGTCCATTGGGATCTTAAGTCTCCAAATTTGTTGGTTGATAAAAATTGGACAGCGAAGGTATGCGATTTTGGGTTGTCCAGATTTAAGGCGAACACTTTCATATCATCCAAGTCCGTTGCTGGAACACCTGAGTGGATGGCTCCAGAATTCCTTCGCGGAGAGCCCTCAAATGAGAAGTCTGATGTTTACAGTTTCGGAGTGATCCTATGGGAACTTGTAACCATGGAACAACCTTGGAACGGTCTTAGCCCCGCCCAGGTAGTTGGAGCCGTTGCTTTCCAGAACAGAAGGCTTGCTATCCCGGCTAATACTCCCCCAGTGTTGGCTTCGCTTATGGAATCCTGCTGGGCTGATGATCCAGCCCAGCGCCCGTCTTTTGCTAGCATAGTTGAGTCGCTAAAGAGGTTGCTAAAGTCTCCTCTGCAGTTGGTACCGGTGGGCGGAACATCCCCCTCTGCCCGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

992

Amino Acids

110.47

Weight (kDa)

6.46

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 296 - 505 1.7e-75 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 716 - 969 4.1e-65 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 716 - 967 9.1e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1973
AarI CACCTGC 1 cut(s) 577
Acc36I ACCTGC 1 cut(s) 577
Acc65I GGTACC 1 cut(s) 2938
AccB1I GGYRCC 3 cut(s) 184, 523, 2938
AccBSI CCGCTC 1 cut(s) 612
AccII CGCG 4 cut(s) 387, 441, 1226, 2664
AclWI GGATC 9 cut(s) 51, 345, 444, 1661, 2215, 2439, 2526, 2704, 2858
AcoI YGGCCR 5 cut(s) 153, 300, 370, 801, 2466
AcsI RAATTY 3 cut(s) 1572, 2533, 2654
AcuI CTGAAG 1 cut(s) 1683
AcyI GRCGYC 1 cut(s) 524
AdeI CACNNNGTG 1 cut(s) 1826
AfaI GTAC 7 cut(s) 260, 346, 1162, 1926, 2009, 2087, 2940
AflII CTTAAG 1 cut(s) 2522
AflIII ACRYGT 1 cut(s) 2296
AgeI ACCGGT 2 cut(s) 1425, 2941
AgsI TTSAA 8 cut(s) 1124, 1354, 1451, 1571, 2092, 2099, 2146, 2263
AjnI CCWGG 5 cut(s) 1057, 1501, 1726, 1830, 2763
AleI CACNNNNGTG 2 cut(s) 1650, 2638
AloI GAACNNNNNNTCC 2 cut(s) 35, 67
Alw21I GWGCWC 2 cut(s) 1302, 1657
Alw26I GTCTC 7 cut(s) 765, 851, 884, 1058, 2109, 2532, 2928
AlwI GGATC 9 cut(s) 51, 345, 444, 1661, 2215, 2439, 2526, 2704, 2858
AlwNI CAGNNNCTG 1 cut(s) 921
Ama87I CYCGRG 1 cut(s) 28
AoxI GGCC 8 cut(s) 153, 268, 300, 370, 508, 801, 2408, 2466
ApeKI GCWGC 4 cut(s) 451, 790, 921, 1311
ApoI RAATTY 3 cut(s) 1572, 2533, 2654
ArsI GACNNNNNNTTYG 6 cut(s) 560, 592, 691, 723, 2669, 2701
AsiGI ACCGGT 2 cut(s) 1425, 2941
Asp700I GAANNNNTTC 3 cut(s) 1546, 2603, 2658
Asp718I GGTACC 1 cut(s) 2938
AspLEI GCGC 2 cut(s) 526, 2877
AspS9I GGNCC 6 cut(s) 268, 339, 509, 1428, 1517, 2970
AsuC2I CCSGG 4 cut(s) 41, 2419, 2810, 2968
AsuHPI GGTGA 6 cut(s) 1003, 1073, 1415, 1554, 1742, 1847
AsuNHI GCTAGC 1 cut(s) 2888
AvaI CYCGRG 1 cut(s) 28
AvaII GGWCC 4 cut(s) 339, 1428, 1517, 2970
BaeGI GKGCMC 1 cut(s) 189
BalI TGGCCA 3 cut(s) 372, 803, 2468
BanI GGYRCC 3 cut(s) 184, 523, 2938
BanII GRGCYC 1 cut(s) 2673
BauI CACGAG 3 cut(s) 98, 854, 1395
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 1302, 1657
BbvCI CCTCAGC 2 cut(s) 220, 501
BbvI GCAGC 4 cut(s) 438, 802, 908, 1323
BccI CCATC 5 cut(s) 367, 514, 653, 944, 2638
BceAI ACGGC 1 cut(s) 2762
BcgI CGANNNNNNTGC 4 cut(s) 529, 563, 1177, 1211
BciT130I CCWGG 5 cut(s) 1059, 1503, 1728, 1832, 2765
BclI TGATCA 3 cut(s) 481, 1732, 2254
BcnI CCSGG 4 cut(s) 41, 2419, 2810, 2968
BcoDI GTCTC 7 cut(s) 765, 851, 884, 1058, 2109, 2532, 2928
BfaI CTAG 6 cut(s) 1490, 1740, 1899, 2024, 2459, 2889
BfmI CTRYAG 3 cut(s) 1152, 2358, 2930
BfoI RGCGCY 2 cut(s) 527, 2878
BfrI CTTAAG 1 cut(s) 2522
BfuAI ACCTGC 1 cut(s) 577
BglII AGATCT 3 cut(s) 1237, 2020, 2127
BisI GCNGC 5 cut(s) 153, 452, 791, 922, 1312
BlsI GCNGC 5 cut(s) 154, 453, 792, 923, 1313
BmcAI AGTACT 3 cut(s) 260, 346, 1162
Bme1390I CCNGG 9 cut(s) 41, 1059, 1503, 1728, 1832, 2419, 2765, 2810, 2968
Bme18I GGWCC 4 cut(s) 339, 1428, 1517, 2970
BmeT110I CYCGRG 1 cut(s) 28
BmgT120I GGNCC 6 cut(s) 268, 339, 509, 1428, 1517, 2970
BmiI GGNNCC 8 cut(s) 186, 320, 340, 525, 1500, 2649, 2776, 2940
BmrFI CCNGG 9 cut(s) 41, 1059, 1503, 1728, 1832, 2419, 2765, 2810, 2968
BmrI ACTGGG 1 cut(s) 2819
BmsI GCATC 6 cut(s) 874, 1345, 1755, 1828, 1889, 2197
BmtI GCTAGC 1 cut(s) 2892
BmuI ACTGGG 1 cut(s) 2819
BpiI GAAGAC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 745, 777
BpmI CTGGAG 1 cut(s) 2634
Bpu10I CCTNAGC 2 cut(s) 220, 501
BpuEI CTTGAG 4 cut(s) 804, 893, 1097, 2144
BpuMI CCSGG 4 cut(s) 41, 2419, 2810, 2968
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 480
BsaHI GRCGYC 1 cut(s) 524
BsaWI WCCGGW 3 cut(s) 558, 1425, 2941
BsaXI ACNNNNNCTCC 2 cut(s) 1639, 1669
Bse118I RCCGGY 3 cut(s) 582, 1425, 2941
Bse1I ACTGG 5 cut(s) 320, 342, 1716, 2123, 2825
Bse3DI GCAATG 3 cut(s) 1006, 1795, 2118
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 5 cut(s) 1059, 1503, 1728, 1832, 2765
BseCI ATCGAT 1 cut(s) 276
BseGI GGATG 9 cut(s) 664, 682, 978, 1815, 2218, 2305, 2612, 2649, 2954
BseJI GATNNNNATC 1 cut(s) 480
BseMI GCAATG 3 cut(s) 1006, 1795, 2118
BseMII CTCAG 6 cut(s) 234, 492, 654, 776, 879, 2628
BseNI ACTGG 5 cut(s) 320, 342, 1716, 2123, 2825
BseRI GAGGAG 5 cut(s) 740, 1516, 1647, 2452, 2916
BseSI GKGCMC 1 cut(s) 189
BseXI GCAGC 4 cut(s) 438, 802, 908, 1323
BseYI CCCAGC 2 cut(s) 2853, 2871
Bsh1236I CGCG 4 cut(s) 387, 441, 1226, 2664
BshFI GGCC 8 cut(s) 155, 270, 302, 372, 510, 803, 2410, 2468
BshNI GGYRCC 3 cut(s) 184, 523, 2938
BshTI ACCGGT 2 cut(s) 1425, 2941
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 1302, 1657
BsiHKCI CYCGRG 1 cut(s) 28
BsiSI CCGG 9 cut(s) 41, 527, 559, 583, 1426, 2418, 2810, 2942, 2968
BslFI GGGAC 4 cut(s) 352, 577, 2150, 2194
BsmAI GTCTC 7 cut(s) 765, 851, 884, 1058, 2109, 2532, 2928
BsmBI CGTCTC 2 cut(s) 884, 2109
BsmFI GGGAC 4 cut(s) 352, 577, 2150, 2194
BsnI GGCC 8 cut(s) 155, 270, 302, 372, 510, 803, 2410, 2468
BsoBI CYCGRG 1 cut(s) 28
Bsp1286I GDGCHC 4 cut(s) 189, 1302, 1657, 2673
Bsp1407I TGTACA 2 cut(s) 1924, 2007
Bsp19I CCATGG 1 cut(s) 2730
Bsp68I TCGCGA 1 cut(s) 441
BspANI GGCC 8 cut(s) 155, 270, 302, 372, 510, 803, 2410, 2468
BspCNI CTCAG 6 cut(s) 233, 493, 653, 775, 880, 2629
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 4 cut(s) 387, 441, 1226, 2664
BspHI TCATGA 2 cut(s) 325, 472
BspLI GGNNCC 8 cut(s) 186, 320, 340, 525, 1500, 2649, 2776, 2940
BspMAI CTGCAG 1 cut(s) 2934
BspMI ACCTGC 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 2892
BspPI GGATC 9 cut(s) 51, 345, 444, 1661, 2215, 2439, 2526, 2704, 2858
BspT107I GGYRCC 3 cut(s) 184, 523, 2938
BspTI CTTAAG 1 cut(s) 2522
BsrBI CCGCTC 1 cut(s) 612
BsrDI GCAATG 3 cut(s) 1006, 1795, 2118
BsrFI RCCGGY 3 cut(s) 582, 1425, 2941
BsrGI TGTACA 2 cut(s) 1924, 2007
BsrI ACTGG 5 cut(s) 320, 342, 1716, 2123, 2825
BssAI RCCGGY 3 cut(s) 582, 1425, 2941
BssNI GRCGYC 1 cut(s) 524
BssSI CACGAG 3 cut(s) 98, 854, 1395
BssT1I CCWWGG 5 cut(s) 2081, 2378, 2469, 2730, 2741
Bst2BI CACGAG 3 cut(s) 98, 854, 1395
Bst2UI CCWGG 5 cut(s) 1059, 1503, 1728, 1832, 2765
Bst4CI ACNGT 9 cut(s) 377, 895, 1029, 1982, 2103, 2185, 2236, 2699, 2750
Bst6I CTCTTC 2 cut(s) 418, 717
BstACI GRCGYC 1 cut(s) 524
BstAFI CTTAAG 1 cut(s) 2522
BstAUI TGTACA 2 cut(s) 1924, 2007
BstDEI CTNAG 9 cut(s) 220, 501, 640, 762, 771, 888, 1904, 2637, 2753
BstDSI CCRYGG 2 cut(s) 1224, 2730
BstF5I GGATG 9 cut(s) 664, 682, 978, 1815, 2218, 2305, 2612, 2649, 2954
BstFNI CGCG 4 cut(s) 387, 441, 1226, 2664
BstH2I RGCGCY 2 cut(s) 527, 2878
BstHHI GCGC 2 cut(s) 526, 2877
BstMAI GTCTC 7 cut(s) 765, 851, 884, 1058, 2109, 2532, 2928
BstMWI GCNNNNNNNGC 7 cut(s) 451, 523, 618, 1333, 1847, 1871, 2810
BstNI CCWGG 5 cut(s) 1059, 1503, 1728, 1832, 2765
BstNSI RCATGY 1 cut(s) 1249
BstSCI CCNGG 9 cut(s) 39, 1057, 1501, 1726, 1830, 2417, 2763, 2808, 2966
BstSFI CTRYAG 3 cut(s) 1152, 2358, 2930
BstSLI GKGCMC 1 cut(s) 189
BstUI CGCG 4 cut(s) 387, 441, 1226, 2664
BstV1I GCAGC 4 cut(s) 438, 802, 908, 1323
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 5 cut(s) 1237, 1666, 2020, 2127, 2518
BstXI CCANNNNNNTGG 3 cut(s) 208, 987, 2771
BstYI RGATCY 5 cut(s) 1237, 1666, 2020, 2127, 2518
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 8 cut(s) 155, 270, 302, 372, 510, 803, 2410, 2468
BsuTUI ATCGAT 1 cut(s) 276
BtgI CCRYGG 2 cut(s) 1224, 2730
BtsCI GGATG 9 cut(s) 664, 682, 978, 1815, 2218, 2305, 2612, 2649, 2954
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 5 cut(s) 123, 922, 1987, 2190, 2832
BtuMI TCGCGA 1 cut(s) 441
BveI ACCTGC 1 cut(s) 577
CaiI CAGNNNCTG 1 cut(s) 921
CciI TCATGA 2 cut(s) 325, 472
CfoI GCGC 2 cut(s) 526, 2877
Cfr10I RCCGGY 3 cut(s) 582, 1425, 2941
Cfr13I GGNCC 6 cut(s) 268, 339, 509, 1428, 1517, 2970
Cfr42I CCGCGG 1 cut(s) 1227
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 2333
CsiI ACCWGGT 1 cut(s) 1057
Csp6I GTAC 7 cut(s) 259, 345, 1161, 1925, 2008, 2086, 2939
CspAI ACCGGT 2 cut(s) 1425, 2941
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 9 cut(s) 326, 473, 494, 1246, 1514, 2133, 2249, 2326, 2731
CviQI GTAC 7 cut(s) 259, 345, 1161, 1925, 2008, 2086, 2939
DdeI CTNAG 9 cut(s) 220, 501, 640, 762, 771, 888, 1904, 2637, 2753
DinI GGCGCC 1 cut(s) 525
DraIII CACNNNGTG 1 cut(s) 1826
EaeI YGGCCR 5 cut(s) 153, 300, 370, 801, 2466
Eam1104I CTCTTC 2 cut(s) 418, 717
EarI CTCTTC 2 cut(s) 418, 717
EciI GGCGGA 1 cut(s) 2964
Eco130I CCWWGG 5 cut(s) 2081, 2378, 2469, 2730, 2741
Eco24I GRGCYC 1 cut(s) 2673
Eco32I GATATC 1 cut(s) 945
Eco47I GGWCC 4 cut(s) 339, 1428, 1517, 2970
Eco57I CTGAAG 1 cut(s) 1683
Eco88I CYCGRG 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 2654
EcoRII CCWGG 5 cut(s) 1057, 1501, 1726, 1830, 2763
EcoRV GATATC 1 cut(s) 945
EcoT14I CCWWGG 5 cut(s) 2081, 2378, 2469, 2730, 2741
EcoT38I GRGCYC 1 cut(s) 2673
EgeI GGCGCC 1 cut(s) 525
EheI GGCGCC 1 cut(s) 525
ErhI CCWWGG 5 cut(s) 2081, 2378, 2469, 2730, 2741
Esp3I CGTCTC 2 cut(s) 884, 2109
FaeI CATG 9 cut(s) 329, 476, 497, 1249, 1517, 2136, 2252, 2329, 2734
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 4 cut(s) 352, 577, 2150, 2194
FatI CATG 9 cut(s) 325, 472, 493, 1245, 1513, 2132, 2248, 2325, 2730
FauI CCCGC 3 cut(s) 605, 1231, 2767
FbaI TGATCA 3 cut(s) 481, 1732, 2254
Fnu4HI GCNGC 5 cut(s) 153, 452, 791, 922, 1312
FokI GGATG 9 cut(s) 671, 689, 985, 1802, 2225, 2292, 2599, 2656, 2941
FriOI GRGCYC 1 cut(s) 2673
Fsp4HI GCNGC 5 cut(s) 153, 452, 791, 922, 1312
FspBI CTAG 6 cut(s) 1490, 1740, 1899, 2024, 2459, 2889
GlaI GCGC 2 cut(s) 525, 2876
GluI GCNGC 5 cut(s) 153, 452, 791, 922, 1312
GsaI CCCAGC 2 cut(s) 2857, 2875
GsuI CTGGAG 1 cut(s) 2634
HaeII RGCGCY 2 cut(s) 527, 2878
HaeIII GGCC 8 cut(s) 155, 270, 302, 372, 510, 803, 2410, 2468
HapII CCGG 9 cut(s) 41, 527, 559, 583, 1426, 2418, 2810, 2942, 2968
HgaI GACGC 1 cut(s) 2333
HhaI GCGC 2 cut(s) 526, 2877
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 9 cut(s) 329, 476, 497, 1249, 1517, 2136, 2252, 2329, 2734
Hin6I GCGC 2 cut(s) 524, 2875
HinP1I GCGC 2 cut(s) 524, 2875
HincII GTYRAC 3 cut(s) 430, 568, 1486
HindII GTYRAC 3 cut(s) 430, 568, 1486
HindIII AAGCTT 2 cut(s) 1190, 1932
HpaII CCGG 9 cut(s) 41, 527, 559, 583, 1426, 2418, 2810, 2942, 2968
HphI GGTGA 6 cut(s) 1003, 1073, 1415, 1554, 1742, 1847
Hpy166II GTNNAC 6 cut(s) 430, 534, 568, 1486, 2042, 2695
Hpy8I GTNNAC 6 cut(s) 430, 534, 568, 1486, 2042, 2695
Hpy99I CGWCG 7 cut(s) 64, 387, 462, 652, 655, 700, 703
HpyCH4III ACNGT 9 cut(s) 377, 895, 1029, 1982, 2103, 2185, 2236, 2699, 2750
HpyCH4IV ACGT 4 cut(s) 653, 1104, 2296, 2313
HpyF10VI GCNNNNNNNGC 7 cut(s) 451, 523, 618, 1333, 1847, 1871, 2810
HpyF3I CTNAG 9 cut(s) 220, 501, 640, 762, 771, 888, 1904, 2637, 2753
HpySE526I ACGT 4 cut(s) 653, 1104, 2296, 2313
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 9 cut(s) 329, 476, 497, 1249, 1517, 2136, 2252, 2329, 2734
HspAI GCGC 2 cut(s) 524, 2875
KasI GGCGCC 1 cut(s) 523
KpnI GGTACC 1 cut(s) 2942
Ksp22I TGATCA 3 cut(s) 481, 1732, 2254
KspI CCGCGG 1 cut(s) 1227
LmnI GCTCC 8 cut(s) 630, 1117, 1228, 1305, 1660, 1795, 2653, 2774
Lsp1109I GCAGC 4 cut(s) 438, 802, 908, 1323
LweI GCATC 6 cut(s) 874, 1345, 1755, 1828, 1889, 2197
MabI ACCWGGT 1 cut(s) 1057
MaeI CTAG 6 cut(s) 1490, 1740, 1899, 2024, 2459, 2889
MaeII ACGT 4 cut(s) 653, 1104, 2296, 2313
MbiI CCGCTC 1 cut(s) 612
MfeI CAATTG 1 cut(s) 2258
MflI RGATCY 5 cut(s) 1237, 1666, 2020, 2127, 2518
MhlI GDGCHC 4 cut(s) 189, 1302, 1657, 2673
MlsI TGGCCA 3 cut(s) 372, 803, 2468
MluNI TGGCCA 3 cut(s) 372, 803, 2468
Mly113I GGCGCC 1 cut(s) 524
MlyI GAGTC 4 cut(s) 539, 680, 1517, 2909
MmeI TCCRAC 4 cut(s) 636, 1232, 1731, 2752
Mox20I TGGCCA 3 cut(s) 372, 803, 2468
MroXI GAANNNNTTC 3 cut(s) 1546, 2603, 2658
MscI TGGCCA 3 cut(s) 372, 803, 2468
MseI TTAA 9 cut(s) 681, 1044, 1053, 1443, 1533, 2223, 2496, 2523, 2592
MslI CAYNNNNRTG 6 cut(s) 276, 471, 474, 477, 1650, 2638
Msp20I TGGCCA 3 cut(s) 372, 803, 2468
MspA1I CMGCKG 2 cut(s) 73, 1226
MspCI CTTAAG 1 cut(s) 2522
MspI CCGG 9 cut(s) 41, 527, 559, 583, 1426, 2418, 2810, 2942, 2968
MspR9I CCNGG 9 cut(s) 41, 1059, 1503, 1728, 1832, 2419, 2765, 2810, 2968
MunI CAATTG 1 cut(s) 2258
MvaI CCWGG 5 cut(s) 1059, 1503, 1728, 1832, 2765
MvnI CGCG 4 cut(s) 387, 441, 1226, 2664
MwoI GCNNNNNNNGC 7 cut(s) 451, 523, 618, 1333, 1847, 1871, 2810
NarI GGCGCC 1 cut(s) 524
NciI CCSGG 4 cut(s) 41, 2419, 2810, 2968
NcoI CCATGG 1 cut(s) 2730
NheI GCTAGC 1 cut(s) 2888
NlaIII CATG 9 cut(s) 329, 476, 497, 1249, 1517, 2136, 2252, 2329, 2734
NlaIV GGNNCC 8 cut(s) 186, 320, 340, 525, 1500, 2649, 2776, 2940
NmeAIII GCCGAG 1 cut(s) 624
NmuCI GTSAC 4 cut(s) 1038, 1811, 2071, 2362
NruI TCGCGA 1 cut(s) 441
NspI RCATGY 1 cut(s) 1249
OliI CACNNNNGTG 2 cut(s) 1650, 2638
PagI TCATGA 2 cut(s) 325, 472
PaqCI CACCTGC 1 cut(s) 577
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 695, 1393
PdmI GAANNNNTTC 3 cut(s) 1546, 2603, 2658
PfeI GAWTC 6 cut(s) 56, 976, 1589, 1754, 2092, 2846
PflFI GACNNNGTC 1 cut(s) 895
PfoI TCCNGGA 1 cut(s) 39
PinAI ACCGGT 2 cut(s) 1425, 2941
PkrI GCNGC 5 cut(s) 154, 453, 792, 923, 1313
PleI GAGTC 4 cut(s) 538, 680, 1517, 2908
PluTI GGCGCC 1 cut(s) 527
PpsI GAGTC 4 cut(s) 538, 680, 1517, 2908
PsiI TTATAA 1 cut(s) 1973
Psp6I CCWGG 5 cut(s) 1057, 1501, 1726, 1830, 2763
PspFI CCCAGC 2 cut(s) 2853, 2871
PspGI CCWGG 5 cut(s) 1057, 1501, 1726, 1830, 2763
PspN4I GGNNCC 8 cut(s) 186, 320, 340, 525, 1500, 2649, 2776, 2940
PspPI GGNCC 6 cut(s) 268, 339, 509, 1428, 1517, 2970
PstI CTGCAG 1 cut(s) 2934
PstNI CAGNNNCTG 1 cut(s) 921
PsuI RGATCY 5 cut(s) 1237, 1666, 2020, 2127, 2518
PsyI GACNNNGTC 1 cut(s) 895
PvuII CAGCTG 1 cut(s) 73
RruI TCGCGA 1 cut(s) 441
RsaI GTAC 7 cut(s) 260, 346, 1162, 1926, 2009, 2087, 2940
RsaNI GTAC 7 cut(s) 259, 345, 1161, 1925, 2008, 2086, 2939
RseI CAYNNNNRTG 6 cut(s) 276, 471, 474, 477, 1650, 2638
SacII CCGCGG 1 cut(s) 1227
SaqAI TTAA 9 cut(s) 681, 1044, 1053, 1443, 1533, 2223, 2496, 2523, 2592
SatI GCNGC 5 cut(s) 153, 452, 791, 922, 1312
Sau96I GGNCC 6 cut(s) 268, 339, 509, 1428, 1517, 2970
ScaI AGTACT 3 cut(s) 260, 346, 1162
SchI GAGTC 4 cut(s) 539, 680, 1517, 2909
ScrFI CCNGG 9 cut(s) 41, 1059, 1503, 1728, 1832, 2419, 2765, 2810, 2968
SduI GDGCHC 4 cut(s) 189, 1302, 1657, 2673
SexAI ACCWGGT 1 cut(s) 1057
SfaNI GCATC 6 cut(s) 874, 1345, 1755, 1828, 1889, 2197
SfcI CTRYAG 3 cut(s) 1152, 2358, 2930
SfoI GGCGCC 1 cut(s) 525
Sfr303I CCGCGG 1 cut(s) 1227
SgrBI CCGCGG 1 cut(s) 1227
SinI GGWCC 4 cut(s) 339, 1428, 1517, 2970
SmiMI CAYNNNNRTG 6 cut(s) 276, 471, 474, 477, 1650, 2638
SmlI CTYRAG 5 cut(s) 819, 872, 1112, 2123, 2522
SmoI CTYRAG 5 cut(s) 819, 872, 1112, 2123, 2522
SspDI GGCGCC 1 cut(s) 523
SspMI CTAG 6 cut(s) 1490, 1740, 1899, 2024, 2459, 2889
StyD4I CCNGG 9 cut(s) 39, 1057, 1501, 1726, 1830, 2417, 2763, 2808, 2966
StyI CCWWGG 5 cut(s) 2081, 2378, 2469, 2730, 2741
TaaI ACNGT 9 cut(s) 377, 895, 1029, 1982, 2103, 2185, 2236, 2699, 2750
TaiI ACGT 4 cut(s) 656, 1107, 2299, 2316
TaqI TCGA 8 cut(s) 46, 54, 276, 349, 362, 608, 1089, 1529
TatI WGTACW 5 cut(s) 258, 344, 1160, 1924, 2007
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 6 cut(s) 56, 976, 1589, 1754, 2092, 2846
Tru1I TTAA 9 cut(s) 681, 1044, 1053, 1443, 1533, 2223, 2496, 2523, 2592
Tru9I TTAA 9 cut(s) 681, 1044, 1053, 1443, 1533, 2223, 2496, 2523, 2592
TscAI CASTG 5 cut(s) 130, 922, 1987, 2190, 2832
TseFI GTSAC 4 cut(s) 1038, 1811, 2071, 2362
TseI GCWGC 4 cut(s) 451, 790, 921, 1311
Tsp45I GTSAC 4 cut(s) 1038, 1811, 2071, 2362
TspDTI ATGAA 8 cut(s) 294, 314, 989, 1502, 2024, 2306, 2314, 2596
TspGWI ACGGA 2 cut(s) 1551, 2611
TspRI CASTG 5 cut(s) 130, 922, 1987, 2190, 2832
Tth111I GACNNNGTC 1 cut(s) 895
Vha464I CTTAAG 1 cut(s) 2522
VpaK11BI GGWCC 4 cut(s) 339, 1428, 1517, 2970
XapI RAATTY 3 cut(s) 1572, 2533, 2654
XbaI TCTAGA 1 cut(s) 2023
XceI RCATGY 1 cut(s) 1249
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmnI GAANNNNTTC 3 cut(s) 1546, 2603, 2658
XspI CTAG 6 cut(s) 1490, 1740, 1899, 2024, 2459, 2889
ZrmI AGTACT 3 cut(s) 260, 346, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.