FvH4_5g38990

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006060
Physical Location & Seq
Forward (+)
28800905 .. 28804388
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38990.t2

Sequence Viewer

Length: 1221 bp
ATGGTGCCACTAGTTCCTCACTCTGAACCGAACCGTATCCGGATACGAATCAGATCCGTATATATAATCACAAAAGTTGCCCACGCAACGCAACCACCTCCGCCTCACGTGACCCAGCGCCAACATCGAACGCTCTCTCCTTCTTTCTCTCTCTCTCCGTCTCTCCCATTCAATCCCAAACTCTCTCTTTCTCTGCAACCTCCGCGGAATTTGAATCGTCTCTCCGACGCTTCCAGGGTCTATTTGTACATGGCCAAAGTCAACAAGTTGCGCCGCAAATCTGACTCTCAGTCCTTGAGGAGGACACCATATGTGCTAAGGGCTGTTAAAGATGTCTGTGCGAAAAAGAAATCTGCCAAACCATTGAAGAAGAAAGATTGGGAAGATGTGAACTGTTCAGTGTGTATGGAATCTCCTCACAATGCAGTTCTCCTCCTTTGTTCCTCTTATGATAAGGGATGCCGCCCTTATATGTGTGCTACTGACCATCGCTATTCCAACTGTCTTGAACAATACCAAAAAGCATACACAAAGGTATCTTCTATTCAGAATTCGCAAGAGTGGGATTTATCAGTTGGATATTTAGGTTTCAATTCTAGTGAAGGGGAGCCCATTGAGAAAGGGGAGAAACCAGAGCTTCTATGCCCACTTTGTCGTGGGCAGGTTAAGGGTTGCACAGTGGTGGAACGTGCAAGGAAACATCTTAATGCCAAGAAGAGAACTTGCCTGCAGGATAAGTGCTCATTCTCTGGAACTTATAGAGAACTCAAGAAACATGTCAAGTCAAAGCACCCATTGGCACGGCCCCGGGCAGTGGATCCTGTACTGCAAGAAAAATGGAAGAAGCTTGAGGGCGAAAGAGAGAGGAATGATGTAATCAGCACCATAATGTCTTCTACACCAGGGGCAGTGGTCCTTGGGGATTATGTGTTGGAGCCTAACCATGATGGCATTTACAGTGATTCCGATTCTGAACTAGATGACTACATTGATGATTTGGGTTTTGGGCCATTTGTGGGTCTAAGTGGTGCTCTTTTCTCACAGCGGAGGTTTCAAACGGACTATGACTCTTTGGATGATGATGATGATTTTTCACGTGCTGCAGCTTCTATTTCTTCTGTTGCACCTCGACGCAGTTTCCCAAGAAACCGGGTGATATTTGGTAGGCCAAGGCGGCGTCGTAGAAACAGAGAAGAACGTGCACCTGGACGATTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

407

Amino Acids

46.3

Weight (kDa)

9.65

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 130 - 296 2.1e-67 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 652
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 205
AccIII TCCGGA 1 cut(s) 39
AciI CCGC 7 cut(s) 101, 203, 205, 274, 463, 1045, 1174
AclWI GGATC 3 cut(s) 48, 812, 825
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 2 cut(s) 208, 550
AcvI CACGTG 2 cut(s) 109, 1097
AcyI GRCGYC 1 cut(s) 1177
AfaI GTAC 2 cut(s) 248, 825
AfiI CCNNNNNNNGG 3 cut(s) 300, 814, 1016
AflIII ACRYGT 1 cut(s) 775
AgsI TTSAA 6 cut(s) 172, 214, 367, 509, 592, 1055
AhdI GACNNNNNGTC 1 cut(s) 289
AhlI ACTAGT 1 cut(s) 10
AjnI CCWGG 3 cut(s) 233, 901, 1204
AleI CACNNNNGTG 1 cut(s) 680
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 3 cut(s) 637, 847, 1106
AluI AGCT 3 cut(s) 637, 847, 1106
Alw21I GWGCWC 3 cut(s) 743, 1033, 1204
Alw26I GTCTC 2 cut(s) 165, 224
Alw44I GTGCAC 1 cut(s) 1200
AlwI GGATC 3 cut(s) 48, 812, 825
Ama87I CYCGRG 1 cut(s) 807
Aor13HI TCCGGA 1 cut(s) 39
AoxI GGCC 4 cut(s) 252, 803, 1007, 1166
ApaLI GTGCAC 1 cut(s) 1200
ApeKI GCWGC 2 cut(s) 1100, 1103
ApoI RAATTY 2 cut(s) 208, 550
AspLEI GCGC 2 cut(s) 120, 273
AspS9I GGNCC 3 cut(s) 804, 913, 1007
AsuC2I CCSGG 3 cut(s) 808, 809, 1151
AsuHPI GGTGA 1 cut(s) 1165
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 1 cut(s) 913
BaeGI GKGCMC 1 cut(s) 1204
BaeI ACNNNNGTAYC 2 cut(s) 519, 552
BalI TGGCCA 1 cut(s) 254
BamHI GGATCC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 612
BbrPI CACGTG 2 cut(s) 109, 1097
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 3 cut(s) 743, 1033, 1204
BbvI GCAGC 2 cut(s) 1087, 1115
BccI CCATC 2 cut(s) 495, 941
BceAI ACGGC 1 cut(s) 818
BciT130I CCWGG 3 cut(s) 235, 903, 1206
BciVI GTATCC 2 cut(s) 36, 47
BcnI CCSGG 3 cut(s) 808, 809, 1151
BcoDI GTCTC 2 cut(s) 165, 224
BcuI ACTAGT 1 cut(s) 10
BfaI CTAG 3 cut(s) 11, 597, 977
BfmI CTRYAG 2 cut(s) 728, 1101
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 652
BfuI GTATCC 2 cut(s) 36, 47
BglI GCCNNNNNGGC 1 cut(s) 1174
BisI GCNGC 5 cut(s) 274, 463, 1101, 1104, 1175
BlsI GCNGC 5 cut(s) 275, 464, 1102, 1105, 1176
Bme1390I CCNGG 6 cut(s) 235, 808, 809, 903, 1151, 1206
Bme18I GGWCC 1 cut(s) 913
BmeRI GACNNNNNGTC 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 807
BmgT120I GGNCC 3 cut(s) 804, 913, 1007
BmiI GGNNCC 5 cut(s) 6, 609, 806, 819, 936
BmrFI CCNGG 6 cut(s) 235, 808, 809, 903, 1151, 1206
BmsI GCATC 1 cut(s) 449
BpiI GAAGAC 1 cut(s) 885
Bpu10I CCTNAGC 1 cut(s) 317
BpuEI CTTGAG 3 cut(s) 316, 752, 869
BpuMI CCSGG 3 cut(s) 808, 809, 1151
BsaAI YACGTR 2 cut(s) 109, 1097
BsaBI GATNNNNATC 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 1177
BsaJI CCNNGG 7 cut(s) 203, 234, 806, 807, 902, 916, 1169
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 814, 1016
Bse8I GATNNNNATC 1 cut(s) 47
BseAI TCCGGA 1 cut(s) 39
BseBI CCWGG 3 cut(s) 235, 903, 1206
BseDI CCNNGG 7 cut(s) 203, 234, 806, 807, 902, 916, 1169
BseGI GGATG 2 cut(s) 464, 1081
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 3 cut(s) 300, 814, 1016
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 3 cut(s) 313, 405, 422
BseSI GKGCMC 1 cut(s) 1204
BseXI GCAGC 2 cut(s) 1087, 1115
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 205
BshFI GGCC 4 cut(s) 254, 805, 1009, 1168
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 3 cut(s) 743, 1033, 1204
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 3 cut(s) 40, 808, 1150
BslI CCNNNNNNNGG 3 cut(s) 300, 814, 1016
BsmAI GTCTC 2 cut(s) 165, 224
BsmBI CGTCTC 2 cut(s) 165, 224
BsnI GGCC 4 cut(s) 254, 805, 1009, 1168
BsoBI CYCGRG 1 cut(s) 807
Bsp1286I GDGCHC 4 cut(s) 612, 743, 1033, 1204
Bsp13I TCCGGA 1 cut(s) 39
Bsp1407I TGTACA 1 cut(s) 246
Bsp143I GATC 2 cut(s) 53, 817
BspACI CCGC 7 cut(s) 101, 203, 205, 274, 463, 1045, 1174
BspANI GGCC 4 cut(s) 254, 805, 1009, 1168
BspCNI CTCAG 1 cut(s) 301
BspEI TCCGGA 1 cut(s) 39
BspFNI CGCG 1 cut(s) 205
BspLI GGNNCC 5 cut(s) 6, 609, 806, 819, 936
BspMAI CTGCAG 2 cut(s) 732, 1105
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 3 cut(s) 48, 812, 825
BspT107I GGYRCC 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 246
BssECI CCNNGG 7 cut(s) 203, 234, 806, 807, 902, 916, 1169
BssMI GATC 2 cut(s) 53, 817
BssNI GRCGYC 1 cut(s) 1177
BssT1I CCWWGG 2 cut(s) 916, 1169
Bst2UI CCWGG 3 cut(s) 235, 903, 1206
Bst4CI ACNGT 5 cut(s) 35, 395, 503, 679, 959
Bst6I CTCTTC 1 cut(s) 710
BstACI GRCGYC 1 cut(s) 1177
BstAUI TGTACA 1 cut(s) 246
BstBAI YACGTR 2 cut(s) 109, 1097
BstC8I GCNNGC 1 cut(s) 728
BstDEI CTNAG 3 cut(s) 288, 317, 1022
BstDSI CCRYGG 1 cut(s) 203
BstENI CCTNNNNNAGG 1 cut(s) 298
BstF5I GGATG 2 cut(s) 464, 1081
BstFNI CGCG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 273
BstKTI GATC 2 cut(s) 56, 820
BstMAI GTCTC 2 cut(s) 165, 224
BstMBI GATC 2 cut(s) 53, 817
BstMWI GCNNNNNNNGC 2 cut(s) 202, 1174
BstNI CCWGG 3 cut(s) 235, 903, 1206
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 6 cut(s) 233, 806, 807, 901, 1149, 1204
BstSFI CTRYAG 2 cut(s) 728, 1101
BstSLI GKGCMC 1 cut(s) 1204
BstUI CGCG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 1087, 1115
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 2 cut(s) 53, 817
BstYI RGATCY 2 cut(s) 53, 817
BsuI GTATCC 2 cut(s) 36, 47
BsuRI GGCC 4 cut(s) 254, 805, 1009, 1168
BtgI CCRYGG 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 2 cut(s) 464, 1081
BtsI GCAGTG 2 cut(s) 819, 915
BtsIMutI CAGTG 5 cut(s) 405, 684, 819, 915, 964
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 728
CfoI GCGC 2 cut(s) 120, 273
Cfr13I GGNCC 3 cut(s) 804, 913, 1007
Cfr42I CCGCGG 1 cut(s) 206
Cfr9I CCCGGG 1 cut(s) 807
CseI GACGC 3 cut(s) 236, 1140, 1166
Csp6I GTAC 2 cut(s) 247, 824
CviAII CATG 3 cut(s) 250, 776, 944
CviQI GTAC 2 cut(s) 247, 824
DdeI CTNAG 3 cut(s) 288, 317, 1022
DpnI GATC 2 cut(s) 55, 819
DpnII GATC 2 cut(s) 53, 817
DriI GACNNNNNGTC 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 710
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 710
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 916, 1169
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 1 cut(s) 913
Eco72I CACGTG 2 cut(s) 109, 1097
Eco88I CYCGRG 1 cut(s) 807
EcoNI CCTNNNNNAGG 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 550
EcoRII CCWGG 3 cut(s) 233, 901, 1204
EcoT14I CCWWGG 2 cut(s) 916, 1169
EcoT38I GRGCYC 1 cut(s) 612
ErhI CCWWGG 2 cut(s) 916, 1169
Esp3I CGTCTC 2 cut(s) 165, 224
FaeI CATG 3 cut(s) 253, 779, 947
FatI CATG 3 cut(s) 249, 775, 943
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 5 cut(s) 274, 463, 1101, 1104, 1175
FokI GGATG 2 cut(s) 471, 1088
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 5 cut(s) 274, 463, 1101, 1104, 1175
FspBI CTAG 3 cut(s) 11, 597, 977
GlaI GCGC 2 cut(s) 119, 272
GluI GCNGC 5 cut(s) 274, 463, 1101, 1104, 1175
GsaI CCCAGC 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 4 cut(s) 254, 805, 1009, 1168
HapII CCGG 3 cut(s) 40, 808, 1150
HgaI GACGC 3 cut(s) 236, 1140, 1166
HhaI GCGC 2 cut(s) 120, 273
Hin1I GRCGYC 1 cut(s) 1177
Hin1II CATG 3 cut(s) 253, 779, 947
Hin6I GCGC 2 cut(s) 118, 271
HinP1I GCGC 2 cut(s) 118, 271
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 7 cut(s) 48, 214, 284, 410, 962, 968, 1067
HpaII CCGG 3 cut(s) 40, 808, 1150
HphI GGTGA 1 cut(s) 1165
Hpy166II GTNNAC 3 cut(s) 262, 391, 1202
Hpy188I TCNGA 7 cut(s) 25, 53, 226, 283, 549, 967, 973
Hpy188III TCNNGA 4 cut(s) 40, 506, 750, 769
Hpy8I GTNNAC 3 cut(s) 262, 391, 1202
Hpy99I CGWCG 3 cut(s) 230, 1134, 1182
HpyAV CCTTC 2 cut(s) 150, 596
HpyCH4III ACNGT 5 cut(s) 35, 395, 503, 679, 959
HpyCH4IV ACGT 4 cut(s) 108, 688, 1096, 1198
HpyCH4V TGCA 9 cut(s) 196, 425, 675, 692, 730, 829, 1103, 1124, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 1174
HpyF3I CTNAG 3 cut(s) 288, 317, 1022
HpySE526I ACGT 4 cut(s) 108, 688, 1096, 1198
Hsp92I GRCGYC 1 cut(s) 1177
Hsp92II CATG 3 cut(s) 253, 779, 947
HspAI GCGC 2 cut(s) 118, 271
Kpn2I TCCGGA 1 cut(s) 39
KspI CCGCGG 1 cut(s) 206
Kzo9I GATC 2 cut(s) 53, 817
LmnI GCTCC 2 cut(s) 607, 934
Lsp1109I GCAGC 2 cut(s) 1087, 1115
LweI GCATC 1 cut(s) 449
MaeI CTAG 3 cut(s) 11, 597, 977
MaeII ACGT 4 cut(s) 108, 688, 1096, 1198
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 55, 819
MboI GATC 2 cut(s) 53, 817
MboII GAAGA 9 cut(s) 379, 382, 395, 531, 727, 853, 885, 1107, 1205
MflI RGATCY 2 cut(s) 53, 817
MhlI GDGCHC 4 cut(s) 612, 743, 1033, 1204
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 3 cut(s) 208, 550, 592
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 2 cut(s) 278, 1061
MmeI TCCRAC 4 cut(s) 249, 522, 556, 912
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 39
MscI TGGCCA 1 cut(s) 254
MseI TTAA 3 cut(s) 327, 666, 705
MslI CAYNNNNRTG 3 cut(s) 680, 705, 887
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 2 cut(s) 205, 1045
MspI CCGG 3 cut(s) 40, 808, 1150
MspR9I CCNGG 6 cut(s) 235, 808, 809, 903, 1151, 1206
MvaI CCWGG 3 cut(s) 235, 903, 1206
MvnI CGCG 1 cut(s) 205
MwoI GCNNNNNNNGC 2 cut(s) 202, 1174
NciI CCSGG 3 cut(s) 808, 809, 1151
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 53, 817
NlaIII CATG 3 cut(s) 253, 779, 947
NlaIV GGNNCC 5 cut(s) 6, 609, 806, 819, 936
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 779
OliI CACNNNNGTG 1 cut(s) 680
PciI ACATGT 1 cut(s) 775
PfeI GAWTC 5 cut(s) 48, 214, 410, 962, 968
PkrI GCNGC 5 cut(s) 275, 464, 1102, 1105, 1176
PleI GAGTC 2 cut(s) 278, 1061
PmaCI CACGTG 2 cut(s) 109, 1097
PmlI CACGTG 2 cut(s) 109, 1097
PpsI GAGTC 2 cut(s) 278, 1061
Ppu21I YACGTR 2 cut(s) 109, 1097
PscI ACATGT 1 cut(s) 775
Psp6I CCWGG 3 cut(s) 233, 901, 1204
PspCI CACGTG 2 cut(s) 109, 1097
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 3 cut(s) 233, 901, 1204
PspN4I GGNNCC 5 cut(s) 6, 609, 806, 819, 936
PspPI GGNCC 3 cut(s) 804, 913, 1007
PstI CTGCAG 2 cut(s) 732, 1105
PsuI RGATCY 2 cut(s) 53, 817
RsaI GTAC 2 cut(s) 248, 825
RsaNI GTAC 2 cut(s) 247, 824
RseI CAYNNNNRTG 3 cut(s) 680, 705, 887
SacII CCGCGG 1 cut(s) 206
SaqAI TTAA 3 cut(s) 327, 666, 705
SatI GCNGC 5 cut(s) 274, 463, 1101, 1104, 1175
Sau3AI GATC 2 cut(s) 53, 817
Sau96I GGNCC 3 cut(s) 804, 913, 1007
SbfI CCTGCAGG 1 cut(s) 732
SchI GAGTC 2 cut(s) 278, 1061
ScrFI CCNGG 6 cut(s) 235, 808, 809, 903, 1151, 1206
SdaI CCTGCAGG 1 cut(s) 732
SduI GDGCHC 4 cut(s) 612, 743, 1033, 1204
SfaNI GCATC 1 cut(s) 449
SfcI CTRYAG 2 cut(s) 728, 1101
Sfr303I CCGCGG 1 cut(s) 206
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 1 cut(s) 913
SmaI CCCGGG 1 cut(s) 809
SmiMI CAYNNNNRTG 3 cut(s) 680, 705, 887
SmlI CTYRAG 3 cut(s) 295, 767, 848
SmoI CTYRAG 3 cut(s) 295, 767, 848
SpeI ACTAGT 1 cut(s) 10
Sse8387I CCTGCAGG 1 cut(s) 732
Sse9I AATT 3 cut(s) 208, 550, 592
SsiI CCGC 7 cut(s) 101, 203, 205, 274, 463, 1045, 1174
SspMI CTAG 3 cut(s) 11, 597, 977
StyD4I CCNGG 6 cut(s) 233, 806, 807, 901, 1149, 1204
StyI CCWWGG 2 cut(s) 916, 1169
TaaI ACNGT 5 cut(s) 35, 395, 503, 679, 959
TaiI ACGT 4 cut(s) 111, 691, 1099, 1201
TaqI TCGA 2 cut(s) 127, 1129
TasI AATT 3 cut(s) 208, 550, 592
TatI WGTACW 2 cut(s) 246, 823
TauI GCSGC 3 cut(s) 276, 465, 1177
TfiI GAWTC 5 cut(s) 48, 214, 410, 962, 968
Tru1I TTAA 3 cut(s) 327, 666, 705
Tru9I TTAA 3 cut(s) 327, 666, 705
TscAI CASTG 5 cut(s) 405, 684, 819, 915, 964
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 2 cut(s) 1100, 1103
Tsp45I GTSAC 1 cut(s) 109
TspGWI ACGGA 3 cut(s) 46, 147, 1073
TspMI CCCGGG 1 cut(s) 807
TspRI CASTG 5 cut(s) 405, 684, 819, 915, 964
VneI GTGCAC 1 cut(s) 1200
VpaK11BI GGWCC 1 cut(s) 913
XagI CCTNNNNNAGG 1 cut(s) 298
XapI RAATTY 2 cut(s) 208, 550
XceI RCATGY 1 cut(s) 779
XmaI CCCGGG 1 cut(s) 807
XspI CTAG 3 cut(s) 11, 597, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.