MD08G1172600.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006060
Physical Location & Seq
Reverse (-)
20794500 .. 20797360
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1172600.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGAGTTCCCTCACAATGCTGTTCTCCTCCTTTGTTCCTCTTATAATAAGGGATGTCGCCCATATATGTGTGCCACTGGCCATCGCTTTTCAAACTGTCTTGAACAATACAAGAAAGCGTACACAAAAGTGTGTTCAATTGAAAATTCTCGACCATGGAATGGACCAATGGATAATTTAGGTTTCAGTTCAACTGAAGGGGAGCCCGAGGAGAAGACAGATGTACCAGAGCTTTTATGCCCGCTATGTCGGGGGCAAGTGAAGGGTTGGACAGTGGTGGAACCAGCACGACAATATTTTGATGCCAAGAAGAGAACATGCTTGCAGGAAAATTGCTCATTTGCTGGAACTTATAAACAGCTCAGAAAACATGTTAAGGCAAAGCACCCATTGGCACGACCCCGAGAAGTGGATCCCTTACTTCAAGAAAAATGGAGGAAGCTTGAGTCTGAGAGAGAGCGAAGTGATGTTATCAGCACAATAGTGTCATCTACTCCGGGGGCAGTGATCATGGGGGATTATGTGCTGGAGCCAAACCCTGATGGCATTTACAGTGATTCTGATTCGGAGCTAGATGATGACAGCGACTCAGATGATTTTGGATTTGGTTCATTTGATATTGGTGGTCTCTTCTCACGACGCACGATTCGAAGGAACAATGATTTTTCGCGTGCTCCGCCTTCTAATTCTGCCTTTGCACCTCGACGTGGTTCTGTACGAGGCAGGGGAGTGGTACGAAGGCTTGGCGGGCTTAGAGGTGGGGGTGTGCAGCGTAGAATTAGAGAACCTGGACGATTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

268

Amino Acids

30.08

Weight (kDa)

8.94

Isoelectric Point (pI)

55.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 1 - 159 7.6e-65 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 45, 354
AccB7I CCANNNNNTGG 1 cut(s) 161
AccII CGCG 1 cut(s) 670
AciI CCGC 3 cut(s) 242, 677, 747
AclWI GGATC 2 cut(s) 407, 420
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 4 cut(s) 122, 225, 717, 735
AfiI CCNNNNNNNGG 3 cut(s) 161, 409, 707
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 6 cut(s) 93, 104, 138, 143, 192, 425
AjiI CACGTC 1 cut(s) 707
AjnI CCWGG 1 cut(s) 787
AleI CACNNNNGTG 2 cut(s) 128, 482
AluBI AGCT 4 cut(s) 232, 361, 442, 571
AluI AGCT 4 cut(s) 232, 361, 442, 571
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 1 cut(s) 632
AlwI GGATC 2 cut(s) 407, 420
Ama87I CYCGRG 2 cut(s) 206, 402
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 769
ApoI RAATTY 1 cut(s) 145
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
AspS9I GGNCC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 498
AsuII TTCGAA 1 cut(s) 649
AvaI CYCGRG 2 cut(s) 206, 402
AvaII GGWCC 1 cut(s) 164
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BalI TGGCCA 1 cut(s) 81
BamHI GGATCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 1 cut(s) 781
BccI CCATC 2 cut(s) 90, 536
BciT130I CCWGG 1 cut(s) 789
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 632
BfaI CTAG 1 cut(s) 572
BisI GCNGC 1 cut(s) 770
BlsI GCNGC 1 cut(s) 771
Bme1390I CCNGG 2 cut(s) 498, 789
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 2 cut(s) 206, 402
BmgBI CACGTC 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 4 cut(s) 204, 282, 414, 531
BmrFI CCNGG 2 cut(s) 498, 789
BmsI GCATC 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 548
Bpu14I TTCGAA 1 cut(s) 649
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 632
BsaJI CCNNGG 3 cut(s) 155, 207, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 409, 707
Bse1I ACTGG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 3 cut(s) 155, 207, 497
BseGI GGATG 1 cut(s) 59
BseLI CCNNNNNNNGG 3 cut(s) 161, 409, 707
BseMII CTCAG 3 cut(s) 376, 441, 603
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 2 cut(s) 17, 224
BseXI GCAGC 1 cut(s) 781
BsgI GTGCAG 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 670
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 676
BsiHKCI CYCGRG 2 cut(s) 206, 402
BsiSI CCGG 1 cut(s) 497
BslI CCNNNNNNNGG 3 cut(s) 161, 409, 707
BsmAI GTCTC 1 cut(s) 632
BsnI GGCC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 632
BsoBI CYCGRG 2 cut(s) 206, 402
Bsp119I TTCGAA 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 207, 676
Bsp143I GATC 2 cut(s) 412, 507
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 3 cut(s) 242, 677, 747
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 3 cut(s) 375, 442, 602
BspFNI CGCG 1 cut(s) 670
BspLI GGNNCC 4 cut(s) 204, 282, 414, 531
BspPI GGATC 2 cut(s) 407, 420
BspT104I TTCGAA 1 cut(s) 649
BspTNI GGTCTC 1 cut(s) 632
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 3 cut(s) 155, 207, 497
BssMI GATC 2 cut(s) 412, 507
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 3 cut(s) 98, 274, 554
Bst6I CTCTTC 2 cut(s) 305, 635
BstBI TTCGAA 1 cut(s) 649
BstC8I GCNNGC 4 cut(s) 242, 323, 672, 749
BstDEI CTNAG 4 cut(s) 362, 450, 589, 752
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 59
BstFNI CGCG 1 cut(s) 670
BstKTI GATC 2 cut(s) 415, 510
BstMAI GTCTC 1 cut(s) 632
BstMBI GATC 2 cut(s) 412, 507
BstMWI GCNNNNNNNGC 2 cut(s) 676, 748
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 2 cut(s) 321, 374
BstSCI CCNGG 2 cut(s) 496, 787
BstUI CGCG 1 cut(s) 670
BstV1I GCAGC 1 cut(s) 781
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 155
BtgZI GCGATG 1 cut(s) 68
BtrI CACGTC 1 cut(s) 707
BtsCI GGATG 1 cut(s) 59
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 4 cut(s) 75, 279, 510, 559
Cac8I GCNNGC 4 cut(s) 242, 323, 672, 749
Cfr13I GGNCC 1 cut(s) 164
CseI GACGC 1 cut(s) 648
Csp6I GTAC 4 cut(s) 121, 224, 716, 734
CviAII CATG 4 cut(s) 156, 318, 371, 511
CviJI RGCY 9 cut(s) 81, 205, 232, 361, 442, 532, 571, 742, 751
CviKI_1 RGCY 9 cut(s) 81, 205, 232, 361, 442, 532, 571, 742, 751
CviQI GTAC 4 cut(s) 121, 224, 716, 734
DdeI CTNAG 4 cut(s) 362, 450, 589, 752
DpnI GATC 2 cut(s) 414, 509
DpnII GATC 2 cut(s) 412, 507
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 305, 635
EarI CTCTTC 2 cut(s) 305, 635
EciI GGCGGA 1 cut(s) 666
Eco130I CCWWGG 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 216
Eco88I CYCGRG 2 cut(s) 206, 402
EcoRII CCWGG 1 cut(s) 787
EcoT14I CCWWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 4 cut(s) 159, 321, 374, 514
FatI CATG 4 cut(s) 155, 317, 370, 510
FauI CCCGC 2 cut(s) 249, 740
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 1 cut(s) 770
FokI GGATG 1 cut(s) 66
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 770
FspBI CTAG 1 cut(s) 572
GluI GCNGC 1 cut(s) 770
GsuI CTGGAG 1 cut(s) 548
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 497
HgaI GACGC 1 cut(s) 648
Hin1II CATG 4 cut(s) 159, 321, 374, 514
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 6 cut(s) 446, 557, 563, 587, 646, 795
HpaII CCGG 1 cut(s) 497
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 5 cut(s) 365, 451, 562, 568, 592
Hpy188III TCNNGA 4 cut(s) 101, 150, 425, 636
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 2 cut(s) 642, 708
HpyAV CCTTC 5 cut(s) 191, 256, 645, 690, 732
HpyCH4III ACNGT 3 cut(s) 98, 274, 554
HpyCH4IV ACGT 1 cut(s) 706
HpyCH4V TGCA 3 cut(s) 325, 698, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 676, 748
HpyF3I CTNAG 4 cut(s) 362, 450, 589, 752
HpySE526I ACGT 1 cut(s) 706
Hsp92II CATG 4 cut(s) 159, 321, 374, 514
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 2 cut(s) 412, 507
LmnI GCTCC 4 cut(s) 202, 529, 568, 679
Lsp1109I GCAGC 1 cut(s) 781
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 706
MalI GATC 2 cut(s) 414, 509
MboI GATC 2 cut(s) 412, 507
MboII GAAGA 3 cut(s) 226, 322, 622
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 207, 676
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 6 cut(s) 138, 145, 175, 331, 685, 777
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 2 cut(s) 455, 581
MmeI TCCRAC 1 cut(s) 248
MnlI CCTC 8 cut(s) 21, 38, 49, 202, 429, 711, 713, 749
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 3 cut(s) 67, 128, 482
Msp20I TGGCCA 1 cut(s) 81
MspI CCGG 1 cut(s) 497
MspR9I CCNGG 2 cut(s) 498, 789
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 1 cut(s) 670
MwoI GCNNNNNNNGC 2 cut(s) 676, 748
NciI CCSGG 1 cut(s) 498
NcoI CCATGG 1 cut(s) 155
NdeII GATC 2 cut(s) 412, 507
NlaIII CATG 4 cut(s) 159, 321, 374, 514
NlaIV GGNNCC 4 cut(s) 204, 282, 414, 531
NspI RCATGY 2 cut(s) 321, 374
NspV TTCGAA 1 cut(s) 649
OliI CACNNNNGTG 2 cut(s) 128, 482
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 646
PfeI GAWTC 4 cut(s) 557, 563, 646, 795
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 1 cut(s) 771
PleI GAGTC 2 cut(s) 454, 581
PpsI GAGTC 2 cut(s) 454, 581
PscI ACATGT 1 cut(s) 370
PsiI TTATAA 2 cut(s) 45, 354
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 4 cut(s) 204, 282, 414, 531
PspPI GGNCC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 4 cut(s) 122, 225, 717, 735
RsaNI GTAC 4 cut(s) 121, 224, 716, 734
RseI CAYNNNNRTG 3 cut(s) 67, 128, 482
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 770
Sau3AI GATC 2 cut(s) 412, 507
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 2 cut(s) 455, 581
ScrFI CCNGG 2 cut(s) 498, 789
SduI GDGCHC 2 cut(s) 207, 676
SetI ASST 9 cut(s) 184, 234, 363, 444, 573, 703, 709, 760, 790
SfaNI GCATC 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 649
SinI GGWCC 1 cut(s) 164
SmiMI CAYNNNNRTG 3 cut(s) 67, 128, 482
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 6 cut(s) 138, 145, 175, 331, 685, 777
SsiI CCGC 3 cut(s) 242, 677, 747
SspI AATATT 1 cut(s) 296
SspMI CTAG 1 cut(s) 572
StyD4I CCNGG 2 cut(s) 496, 787
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 3 cut(s) 98, 274, 554
TaiI ACGT 1 cut(s) 709
TaqI TCGA 3 cut(s) 151, 649, 703
TasI AATT 6 cut(s) 138, 145, 175, 331, 685, 777
TfiI GAWTC 4 cut(s) 557, 563, 646, 795
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 4 cut(s) 82, 279, 510, 559
TseI GCWGC 1 cut(s) 769
TspDTI ATGAA 1 cut(s) 600
TspRI CASTG 4 cut(s) 82, 279, 510, 559
Van91I CCANNNNNTGG 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 1 cut(s) 145
XceI RCATGY 2 cut(s) 321, 374
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.