pycom08g14740

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006060
Physical Location & Seq
Reverse (-)
14138051 .. 14138881
831 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14740.1

Sequence Viewer

Length: 831 bp
ATGGAGTTCCCTCACAATGCTGTTCTCCTCCTTTGTTCCTCTTATAATAAGGGATGTCGCCCATATATGTGTGCCACTGGCCGTCGCTTTTCAAACTGTCTCGAACAATACAAGAAAGCGTACACAAAAGTGTGTTCAATTGAAAATTCTCGACCATGGAATGGAGTCAATGGACCAATGTATAATTTAGGTTTCAGTTCAACTCAAGGGGAGCCCGAGGAGAAGACAGATGTACCAGAGCTTTTATGCCCGCTATGTCGGGGGCAAGTGAAGGGTTGGACAGTGGTGGAACCAGCACGACAATATTTTGATGCCAAGAAGAGAACATGCTTGCAGGAAAATTGCTCATTTGCTGGAACTTATAAACAGCTCAGAAAACATGTTAAGGCAAAGCACCCATTGGCACGACCCCGAGAAGTGGATCCCTTACTTCAAGAAAAATGGAGGAAGCTTGAGTCTGAGAGAGAGCGAAGTGATGTTATCAGCACAATAGTGTCATCTACTCCGGGGGCAGCGATCATGGGGGATTATGTGCTGGAGCCAAACCCTGACGGCATTTACAGTGATTCTGATTCGGAGCTAGATGATGACAGCGACTCAGATGATTTTGGATTTCGTTCGTTTGATATTGGTGGTCTCTTCTCACGACGCATGATTCGAAGGAACAATGATTTTTTGGATGACAATGATTTTTCCCGTGCTCCGCCTTCTAATTCTACCTTTGCATCTCGACGTGGTTCTGTTCGAGGCAGGGGAGTGGTACGAAGGCTTGGCGGGCTTAGAGGTGGGGGTGTGCAGCGTAGAATTAGAGAACCTGGACGATTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

277

Amino Acids

31.25

Weight (kDa)

9.04

Isoelectric Point (pI)

57.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 1 - 162 9e-64 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 45, 363
AccB7I CCANNNNNTGG 1 cut(s) 161
AciI CCGC 3 cut(s) 251, 704, 774
AclWI GGATC 2 cut(s) 416, 429
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 145
AfaI GTAC 3 cut(s) 122, 234, 762
AfiI CCNNNNNNNGG 2 cut(s) 161, 418
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 5 cut(s) 93, 138, 143, 201, 434
AjiI CACGTC 1 cut(s) 734
AjnI CCWGG 1 cut(s) 814
AleI CACNNNNGTG 2 cut(s) 128, 491
AluBI AGCT 4 cut(s) 241, 370, 451, 580
AluI AGCT 4 cut(s) 241, 370, 451, 580
Alw21I GWGCWC 1 cut(s) 703
Alw26I GTCTC 2 cut(s) 104, 641
AlwI GGATC 2 cut(s) 416, 429
Ama87I CYCGRG 2 cut(s) 215, 411
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 512, 796
ApoI RAATTY 1 cut(s) 145
ArsI GACNNNNNNTTYG 2 cut(s) 639, 671
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 507
AsuII TTCGAA 1 cut(s) 658
AvaI CYCGRG 2 cut(s) 215, 411
AvaII GGWCC 1 cut(s) 173
BaeI ACNNNNGTAYC 2 cut(s) 216, 249
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 703
BbvI GCAGC 2 cut(s) 524, 808
BceAI ACGGC 2 cut(s) 66, 568
BciT130I CCWGG 1 cut(s) 816
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 2 cut(s) 104, 641
BfaI CTAG 1 cut(s) 581
BisI GCNGC 2 cut(s) 513, 797
BlsI GCNGC 2 cut(s) 514, 798
Bme1390I CCNGG 2 cut(s) 507, 816
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 2 cut(s) 215, 411
BmgBI CACGTC 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 4 cut(s) 213, 291, 423, 540
BmrFI CCNGG 2 cut(s) 507, 816
BmsI GCATC 2 cut(s) 301, 734
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 557
Bpu14I TTCGAA 1 cut(s) 658
BpuEI CTTGAG 2 cut(s) 189, 473
BpuMI CCSGG 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 3 cut(s) 155, 216, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 418
Bse1I ACTGG 1 cut(s) 82
BseBI CCWGG 1 cut(s) 816
BseDI CCNNGG 3 cut(s) 155, 216, 506
BseGI GGATG 2 cut(s) 59, 685
BseLI CCNNNNNNNGG 2 cut(s) 161, 418
BseMII CTCAG 3 cut(s) 385, 450, 612
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 2 cut(s) 17, 233
BseXI GCAGC 2 cut(s) 524, 808
BsgI GTGCAG 1 cut(s) 815
BshFI GGCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 703
BsiHKCI CYCGRG 2 cut(s) 215, 411
BsiSI CCGG 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 161, 418
BsmAI GTCTC 2 cut(s) 104, 641
BsnI GGCC 1 cut(s) 81
Bso31I GGTCTC 1 cut(s) 641
BsoBI CYCGRG 2 cut(s) 215, 411
Bsp119I TTCGAA 1 cut(s) 658
Bsp1286I GDGCHC 2 cut(s) 216, 703
Bsp143I GATC 2 cut(s) 421, 516
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 3 cut(s) 251, 704, 774
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 3 cut(s) 384, 451, 611
BspLI GGNNCC 4 cut(s) 213, 291, 423, 540
BspPI GGATC 2 cut(s) 416, 429
BspT104I TTCGAA 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 641
BsrI ACTGG 1 cut(s) 82
BssECI CCNNGG 3 cut(s) 155, 216, 506
BssMI GATC 2 cut(s) 421, 516
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 816
Bst4CI ACNGT 3 cut(s) 98, 283, 563
Bst6I CTCTTC 2 cut(s) 314, 644
BstBI TTCGAA 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 251, 332, 776
BstDEI CTNAG 4 cut(s) 371, 459, 598, 779
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 59, 685
BstKTI GATC 2 cut(s) 424, 519
BstMAI GTCTC 2 cut(s) 104, 641
BstMBI GATC 2 cut(s) 421, 516
BstMWI GCNNNNNNNGC 1 cut(s) 775
BstNI CCWGG 1 cut(s) 816
BstNSI RCATGY 2 cut(s) 330, 383
BstSCI CCNGG 2 cut(s) 505, 814
BstV1I GCAGC 2 cut(s) 524, 808
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 155
BtrI CACGTC 1 cut(s) 734
BtsCI GGATG 2 cut(s) 59, 685
BtsIMutI CAGTG 3 cut(s) 75, 288, 568
Cac8I GCNNGC 3 cut(s) 251, 332, 776
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 1 cut(s) 657
Csp6I GTAC 3 cut(s) 121, 233, 761
CviAII CATG 5 cut(s) 156, 327, 380, 520, 652
CviJI RGCY 9 cut(s) 81, 214, 241, 370, 451, 541, 580, 769, 778
CviKI_1 RGCY 9 cut(s) 81, 214, 241, 370, 451, 541, 580, 769, 778
CviQI GTAC 3 cut(s) 121, 233, 761
DdeI CTNAG 4 cut(s) 371, 459, 598, 779
DpnI GATC 2 cut(s) 423, 518
DpnII GATC 2 cut(s) 421, 516
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 2 cut(s) 314, 644
EarI CTCTTC 2 cut(s) 314, 644
EciI GGCGGA 1 cut(s) 693
Eco130I CCWWGG 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 641
Eco47I GGWCC 1 cut(s) 173
Eco88I CYCGRG 2 cut(s) 215, 411
EcoRII CCWGG 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 155
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 5 cut(s) 159, 330, 383, 523, 655
FatI CATG 5 cut(s) 155, 326, 379, 519, 651
FauI CCCGC 2 cut(s) 258, 767
Fnu4HI GCNGC 2 cut(s) 513, 797
FokI GGATG 2 cut(s) 66, 692
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 2 cut(s) 513, 797
FspBI CTAG 1 cut(s) 581
GluI GCNGC 2 cut(s) 513, 797
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 506
HgaI GACGC 1 cut(s) 657
Hin1II CATG 5 cut(s) 159, 330, 383, 523, 655
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 7 cut(s) 165, 455, 566, 572, 596, 655, 822
HpaII CCGG 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 123
Hpy188I TCNGA 5 cut(s) 374, 460, 571, 577, 601
Hpy188III TCNNGA 5 cut(s) 101, 150, 434, 645, 729
Hpy8I GTNNAC 1 cut(s) 123
Hpy99I CGWCG 3 cut(s) 87, 651, 735
HpyAV CCTTC 4 cut(s) 265, 654, 717, 759
HpyCH4III ACNGT 3 cut(s) 98, 283, 563
HpyCH4IV ACGT 1 cut(s) 733
HpyCH4V TGCA 3 cut(s) 334, 725, 796
HpyF10VI GCNNNNNNNGC 1 cut(s) 775
HpyF3I CTNAG 4 cut(s) 371, 459, 598, 779
HpySE526I ACGT 1 cut(s) 733
Hsp92II CATG 5 cut(s) 159, 330, 383, 523, 655
Kzo9I GATC 2 cut(s) 421, 516
LmnI GCTCC 4 cut(s) 211, 538, 577, 706
Lsp1109I GCAGC 2 cut(s) 524, 808
LweI GCATC 2 cut(s) 301, 734
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 733
MalI GATC 2 cut(s) 423, 518
MboI GATC 2 cut(s) 421, 516
MboII GAAGA 3 cut(s) 235, 331, 631
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 216, 703
MluCI AATT 6 cut(s) 138, 145, 184, 340, 712, 804
MlyI GAGTC 3 cut(s) 174, 464, 590
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 7 cut(s) 21, 38, 49, 211, 438, 740, 776
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 3 cut(s) 67, 128, 491
MspI CCGG 1 cut(s) 506
MspR9I CCNGG 2 cut(s) 507, 816
MunI CAATTG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 816
MwoI GCNNNNNNNGC 1 cut(s) 775
NciI CCSGG 1 cut(s) 507
NcoI CCATGG 1 cut(s) 155
NdeII GATC 2 cut(s) 421, 516
NlaIII CATG 5 cut(s) 159, 330, 383, 523, 655
NlaIV GGNNCC 4 cut(s) 213, 291, 423, 540
NspI RCATGY 2 cut(s) 330, 383
NspV TTCGAA 1 cut(s) 658
OliI CACNNNNGTG 2 cut(s) 128, 491
PciI ACATGT 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PfeI GAWTC 4 cut(s) 566, 572, 655, 822
PflMI CCANNNNNTGG 1 cut(s) 161
PkrI GCNGC 2 cut(s) 514, 798
PleI GAGTC 3 cut(s) 173, 463, 590
PpsI GAGTC 3 cut(s) 173, 463, 590
PscI ACATGT 1 cut(s) 379
PsiI TTATAA 2 cut(s) 45, 363
Psp6I CCWGG 1 cut(s) 814
PspGI CCWGG 1 cut(s) 814
PspN4I GGNNCC 4 cut(s) 213, 291, 423, 540
PspPI GGNCC 1 cut(s) 173
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 3 cut(s) 122, 234, 762
RsaNI GTAC 3 cut(s) 121, 233, 761
RseI CAYNNNNRTG 3 cut(s) 67, 128, 491
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 2 cut(s) 513, 797
Sau3AI GATC 2 cut(s) 421, 516
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 3 cut(s) 174, 464, 590
ScrFI CCNGG 2 cut(s) 507, 816
SduI GDGCHC 2 cut(s) 216, 703
SetI ASST 9 cut(s) 193, 243, 372, 453, 582, 722, 736, 787, 817
SfaNI GCATC 2 cut(s) 301, 734
SfuI TTCGAA 1 cut(s) 658
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 3 cut(s) 67, 128, 491
SmlI CTYRAG 2 cut(s) 204, 452
SmoI CTYRAG 2 cut(s) 204, 452
Sse9I AATT 6 cut(s) 138, 145, 184, 340, 712, 804
SsiI CCGC 3 cut(s) 251, 704, 774
SspI AATATT 1 cut(s) 305
SspMI CTAG 1 cut(s) 581
StyD4I CCNGG 2 cut(s) 505, 814
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 3 cut(s) 98, 283, 563
TaiI ACGT 1 cut(s) 736
TaqI TCGA 5 cut(s) 102, 151, 658, 730, 745
TasI AATT 6 cut(s) 138, 145, 184, 340, 712, 804
TfiI GAWTC 4 cut(s) 566, 572, 655, 822
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 3 cut(s) 82, 288, 568
TseI GCWGC 2 cut(s) 512, 796
TspRI CASTG 3 cut(s) 82, 288, 568
Van91I CCANNNNNTGG 1 cut(s) 161
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 1 cut(s) 145
XceI RCATGY 2 cut(s) 330, 383
XspI CTAG 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.